Diversity and time-series analyses of Caribbean deep-sea coral and sponge assemblages on the tropical island slope of Isla de Roatán, Honduras

Shore-based submersible operations, from 2006 to 2020, have allowed us to examine megabenthic assemblages along the island margin of Isla de Roatán from depths of about 150 to 750 m, including repeated observations of the same organisms. These dives were used to photo-document a diverse benthic assemblage and observe the health and condition of the sessile fauna in a well-explored but relatively undocumented area of the Mesoamerican Reef. Samples were collected by dip net, and some dives profiled the water column chemistry in the year 2011. The deep-sea coral assemblage observed off Roatan exhibits high abundance and diversity. The sessile habitat-forming taxa consist primarily of at least 20 different octocorals (e.g., Plexauridae, Primnoidae, Coralliidae, Isididae, and Ellisellidae) and 20 different sponges each (Demospongiae and Hexactinellida), with several known and unknown taxa of Zoantharia, Antipatharia (Bathypathes spp), and Scleractinia (e.g., Desmophyllum pertusum, Dendrophyllia alternata, Madracis myriaster, and solitary taxa). Crinoidea were also abundant and diverse, represented by at least nine species. Epifaunal assemblages associated with corals include at least 24 macroinvertebrate species dominated by Asteroschema laeve (Ophiuroidea) and Chirostylus spp. (Decapoda: Anomura). Repeated observations of a few large octocoral colonies over many years illustrate patterns of predation, recolonization, and epibiont host fidelity, including a 14-year record of decline in a plexaurid octocoral (putatively Paramuricea sp.) and loss of its resident ophiuroids. The shore-based submersible provides a practical and relatively inexpensive platform from which to study coral and sponge assemblages on a deep tropical island slope. The deep-sea coral gardens are likely to harbor new species and new discoveries if more samples can be acquired and made available for taxonomic research.

Experimental Study on 3D Measurement Accuracy Detection of Low Altitude UAV for Repeated Observation of an Invariant Surface

Low-altitude unmanned aerial vehicle (UAV) photogrammetry combined with structure-from-motion (SFM) algorithms is the latest technological approach to imaging 3D stereo constructions. At present, derivative products have been widely used in landslide monitoring, landscape evolution, glacier movement, volume measurement, and landscape change detection. However, there is still a lack of research into the accuracy of 3D data positioning based on the structure-from-motion of unmanned aerial vehicle (UAV-SFM) technology, itself, which can affect the measurable effectiveness of the results in further applications of this technological approach. In this paper, validation work was carried out for the DJI Phantom 4 RTK UAV, for earth observation data related to 3D positioning accuracy. First, a test plot with a relatively stable surface was selected for repeated flight imaging observations. Specifically, three repeated flights were performed on the test plot to obtain three sorties of images; the structure from motion and multi-view stereo (SFM-MVS) key technology was used to process and construct a 3D scene model, and based on this model the digital surface model (DSM) and digital orthophoto map (DOM) data of the same plot with repeated observations were obtained. In order to check the level of 3D measurement accuracy of the UAV technology itself, a window selection-based method was used to sample the point cloud set data from the three-sortie repeat observation 3D model. The DSM and DOM data obtained from three repeated flights over the surface invariant test plots were used to calculate the repeat observation 3D point errors, taking into account the general methodology of redundant observation error analysis for topographic surveys. At the same time, to further analyze the limits of the UAV measurement technique, possible under equivalent observation conditions with the same processing environment, a difference model (DOD) was constructed for the DSM data from three sorties, to deepen the overall characterization of the differences between the DSMs obtained from repeated observations. The results of the experimental study concluded that both the analysis of the 3D point set measurements based on window sampling and the accuracy evaluation using the difference model were generally able to achieve a centimeter level of planimetric accuracy and vertical accuracy. In addition, the accuracy of the surface-stabilized hardened ground was better, overall, than the accuracy of the non-hardened ground. The results of this paper not only probe the measurement limits of this type of UAV, but also provide a quantitative reference for the accurate control and setting of an acquisition scheme of the UAV-based SfM-MVS method for geomorphological data acquisition and 3D reconstruction.

The impact of COVID-19 restrictions on older adults' loneliness: Evidence from high-frequency panel data

BACKGROUND: It is unclear how strong and long lasting the effects of recurring COVID-19 restrictions on older adults' loneliness are. METHODS: 469 retired older adults (60+) provided 8,814 repeated observations of loneliness (27 waves) in the Austrian Corona Panel Project between March 2020 and December 2021. Ordinal mixed regression models were used to estimate the effect of the stringency of COVID-19 restrictions (SI) on loneliness. RESULTS: The proportion of older adults who reported to be often lonely correlated closely (r=0.63) with the SI over time: both peaked during lockdowns (SI=82, often lonely=10-12%) and were lowest during the summer of 2020 (SI=36, often lonely=5- 6%). Results from regression models indicate, that when the SI increased above 60 (=strict lockdown), an increase in loneliness followed. Older adults who lived alone were more affected than those living with others. CONCLUSIONS: Stringent COVID-19 restrictions lead to situational loneliness, par- ticularly among those who lived alone. Efforts should be made to enable older adults who live alone to have save in-person contact during lockdown periods.

Binary fraction of O and B-type stars from LAMOST data

Binary stars play an important role in the evolution of stellar populations . The intrinsic binary fraction (f bin) of O and B-type (OB) stars in LAMOST DR5 was investigated in this work. We employed a cross-correlation approach to estimate relative radial velocities for each of the stellar spectra. The algorithm described by Sana et al. (2013) was implemented and several simulations were made to assess the performance of the approach. The binary fraction of the OB stars is estimated through comparing the uni-distribution between observations and simulations with the Kolmogorov-Smirnov tests. Simulations show that it is reliable for stars most of whom have six, seven and eight repeated observations. The uncertainty of orbital parameters of binarity becomes larger when observational frequencies decrease. By adopting the fixed power exponents of π = −0.45 and κ = −1 for period and mass ratio distributions, respectively, we obtain that f bin = 0.4 − 0.06 + 0.05 for the samples with more than three observations. When we consider the full samples with at least two observations, the binary fraction turns out to be 0.37 − 0.03 + 0.03 . These two results are consistent with each other in 1σ.

Protocol for the study of hepatic bilirubin uptake in the isolated perfused rat liver

We present a protocol for the study of bilirubin uptake in the isolated and perfused rat liver. The liver is perfused with an albumin-free saline buffered solution supplemented with glucose, pyruvate and lactate, in the absence of oxygen, at a physiologically low flow rate. Fractions of the venous effluent are collected and analyzed for bilirubin, bilirubin glucuronide and biomarkers of liver integrity. The liver preparation is viable and intact for 1 h after isolation from the general circulation, with constant levels of both bilirubin and bilirubin glucuronide (< 2 nM) in the effluent. Up to 12 boli of 10 nmol bilirubin can be sequentially injected into the portal vein without and with molecules that target sinusoidal membrane transporters of organic anions. Selective inhibition of bilirubin or bilirubin glucuronide uptake is detected as transient peaks in the effluent (Cmax up 6 to 60 nM). This protocol allows collecting repeated observations in the same liver, thus reducing the animal number by a factor of 10.

New Horizons Detection of the Local Galactic Lyman-α Background

Since 2007 the Alice spectrograph on the New Horizons (NH) spacecraft has been used to periodically observe the Lyman-α (Lyα) emissions of the interplanetary medium (IPM), which mostly result from resonant scattering of solar Lyα emissions by interstellar hydrogen atoms passing through the solar system. Three observations of IPM Lyα along a single great circle were made during the NH cruise to Pluto, and these have been supplemented by observations along six great circles (spread over the sky at 30° intervals), acquired one month before and one day after the NH flyby of Pluto, and on a further five occasions since then, out to just over 47 au from the Sun. These data indicate a distant Lyα background of 43 ± 3 Rayleigh brightness (equivalent to 56 ± 4 nW m−2 sr−1), which is present in all directions (i.e., not only in the upstream direction, as previously reported). This result is found independently by: (1) the falloff with distance from the Sun of the IPM Lyα brightness observed by NH–Alice in several directions on the sky, and (2) the residual between the observed brightness and a model brightness accounting for the resonantly scattered solar Lyα component alone. The repeated observations show that this distant Lyα background is constant and uniform over the sky, and represents the local Galactic Lyα background. The observations show no strong correlation with the cloud structure of the local IPM. The observed brightness constrains the absorption coefficient of interstellar dust at Lyα to 0.2 ± 0.01 kpc−1.