Differences in Genotype and Antimicrobial Resistance between Campylobacter spp. Isolated from Organic and Conventionally Produced Chickens in Sweden

Antibiotic resistance is a major challenge worldwide and increased resistance to quinolones in Campylobacter is being reported. Analysis of antibiotic resistance was performed on 157 Campylobacter strains (123 C. jejuni and 34 C. coli) from conventional and organic chickens produced in Sweden. Susceptibility for tetracycline, ciprofloxacin, erythromycin, nalidixic acid, streptomycin, and gentamycin was determined by microdilution. All 77 isolates from organic chickens were sensitive to all antibiotics, except two C. jejuni that were resistant to tetracycline. Of the 80 isolates from conventional chickens, 22.5% of C. jejuni and 11.1% of C. coli were resistant to quinolones and 5.6% of C. jejuni were resistant to tetracycline. Whole-genome sequencing resulted in 50 different sequence types of C. jejuni and six of C. coli. Nine sequence types were found in both organic and conventional chickens. Two of these (ST-19 and ST-257) included isolates from conventional broilers with different resistance phenotypes to the remaining isolates from conventional and organic broilers. There are management differences between the production systems, such as feed, breed, use of coccidiostats, and access to outdoor area. It is unlikely that quinolone resistance has arisen due to use of antimicrobials, since fluoroquinolones are not permitted in Swedish broiler production.

Longitudinal Sampling Reveals Persistence of and Genetic Diversity in Extended-Spectrum Cephalosporin-Resistant Escherichia coli From Norwegian Broiler Production

There are knowledge gaps concerning dynamics of extended-spectrum cephalosporin (ESC)-resistant Escherichia coli and their plasmids in broiler production and the persistence of strains on broiler farms. Thus, we aimed at characterising ESC-resistant Escherichia coli collected from all flocks reared on 10 different farms during a six-months sampling period. All isolates (n = 43) were subjected to whole-genome sequencing, and a subset of isolates (n = 7) were also sequenced using oxford nanopore technology and subsequent hybrid assembly in order to do in-depth characterisation of the ESC resistance plasmids. The 43 isolates belonged to 11 different sequence types, and three different ESC resistance gene/plasmid combinations were present, namely, IncK2/blaCMY-2 (n = 29), IncI1/blaCMY-2 (n = 6) and IncI1/blaCTX-M-1 (n = 8). ESC-resistant E. coli of different STs and with different ESC resistance gene/plasmid combinations could be present on the same farm, while a single ST and ESC resistance gene/plasmid displaying zero or few SNP differences were present on other farms. In-depth characterisation of IncK2/blaCMY-2 plasmids revealed that at least two distinct variants circulate in the broiler production. These plasmids showed close homology to previously published plasmids from other countries. Our longitudinal study show that ESC-resistant E. coli belong to a multitude of different STs and that different ESC resistance genes and plasmids occur. However, there is also indication of persistence of both ESC-resistant E. coli strains and IncK2/blaCMY-2 plasmids on farms. Further studies are warranted to determine the dynamics of strains, plasmids and ESC resistance genes within single broiler flocks.

Effect of Broiler Prices on Logistics and Supply Chains In Developing The Poultry Industry In Egypt

This study aimed to study the effect of changes of broiler prices on the profitability of broiler logistics under Egyptian conditions. This study was undertaken during the period 2016 – 2020 on random cycles of both broiler and layer farms in three different provinces which were Menofia, Kaliobia and Giza. The data were collected from a cross-sectional survey on the broiler and during the data collection the researcher was contact with the poultry holders and managers. The data were collected from the accurate records which available in the poultry farms of the study areas and from the structured questionnaires methods which established by the researcher and admitted to the farmers during the time of interview, also, the data collected from the Agricultural Directorates of governorates, Livestock development sector and Economic Affairs Sector. The data includes data about logistics costs, returns and net prpfits of broiler production, the data were analyized statistically and economically. The price of broilers considered as the main variable affecting the profitability of broiler production farms. The price of poultry affected by the costs of production costs that includes variable and fixed costs The results, concluded that, the higher prices of broiler prices observed during the years 2018, 2019 and 2020 than that of years 2016 and 2017 and in winter seasons than the summer seasons. The increasing of broiler prices causes increasing returns level for broiler sales, the summer seasons achieved a higher net profit than the winter seasons due to increasing the level of production costs in winter seasons than the summer seasons.

A Mini Review of the Effects of NSP and Exogenous Enzymes in Broiler Diets on Digestibility and Some Intestinal Functions

Summary NSP (non-starch polysaccharides) are a large group of compounds, which vary greatly in their chemical structure and properties. They are linked with the use of some of the alternative proteins or energy feed ingredients in broiler nutrition. Exogenous enzymes which act on NSP are sometimes in broiler production with the purpose of increasing digestibility of nutrients and consequently increasing broiler performance in production. This paper will attempt to review changes occurring in the digestive tract as a result of the use of feed with NSP soluble and insoluble in water, and the effect of NSPase on them, in terms of how an animal organism is burdened or helped to overcome the problems.

Modeling the Growth of Four Commercial Broiler Genotypes Reared in the Tropics

Genetic improvement in commercial broilers worldwide is heavily focused on selection for higher final body weight at a given age. Although commercial broilers are mostly sold by their final body weight, it is important to pay attention to how this weight is attained and at what cost. The cost of feeding broilers, which constitutes about 70% of the total cost of broiler production, varies considerably at different stages of the bird. It is, therefore, important to pay attention to the growth curve of broilers and the parameters of the growth curve to maximise profitability of commercial broiler production. The objective of this study was to model the variations of the growth curves of 4 commercial broiler genotypes reared in Ghana using the Gompertz and polynomial growth functions. Data on body weights at 1, 7, 14, 21, 28, 35 and 42 days for 4 unsexed commercial broiler genotypes were used to model both the Gompertz and polynomial growth functions. The 4 genotypes ranked differently for Gompertz predicted early (1 - 28 days), late growth (28 – 42 days) and body weight at 42 days. Gompertz function predicted growth better for broiler chicken than the polynomial as the parameters of the Gompertz function are biologically meaningful and heritable. Selection of broiler genotypes for production based on their growth curve (slower early growth and faster late growth) could minimize cost of production and thereby increase the profitability of commercial broiler production in the tropics.

Genomic Characterisation of Campylobacter jejuni Isolates Recovered During Commercial Broiler Production

Background:Campylobacter is commonly transmitted to humans from chickens. Campylobacter jejuni is the species most frequently associated with human illness, and the most prevalent species recovered from poultry.Objective: The objective of this study was to analyse a sub-population of C. jejuni from two broiler flocks on the farm and at slaughter using whole-genome sequencing to gain insights into the changes in the Campylobacter population during broiler production, including changes in virulence and antimicrobial resistance profiles.Methods: In this study, ten composite faecal samples (n=10), obtained by pooling ten fresh faecal samples (n=10), were collected in the broiler house on two farms on days 14, 21, 28, and 34 (n=80) and ten composite (n=10) caecal samples were collected at the time of slaughter for each flock (n=20). These were tested for C. jejuni using the ISO 10272-2:2016 method. Seven isolates were randomly selected from each of the nine Campylobacter-positive sampling points (n=63) and were subjected to antimicrobial susceptibility tests. Their genomes were sequenced and the data obtained was used to characterise the population structure, virulence, antimicrobial resistance determinants and inter-strain variation.Results: The Farm 1 isolates had three MLST types (ST257-257, ST814-661 and ST48-48) while those on Farm 2 were ST6209-464 and ST9401. Interestingly, only the MLST types positive for most of the virulence genes tested in this study persisted throughout the production cycle, and the detection of antimicrobial resistance determinants (gyrA T86I and tetO) increased after thinning and at slaughter, with the detection of new strains.Conclusion: The persistence of the most virulent strains detected in this study throughout the production cycle has important implications for the risk to consumers and requires further investigation. The detection of new strains within the population corresponding with the time of thinning and transportation reflects previous reports and provides further evidence that these activities pose a risk of introducing new Campylobacter strains to broiler batches.