raw wastewater
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Water ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 218
Author(s):  
Tiow Ping Wong ◽  
Roger W. Babcock ◽  
Theodore Uekawa ◽  
Joachim Schneider ◽  
Bing Hu

Extracellular polymeric substances (EPS) reportedly make up approximately half of the organic matter in activated sludge (AS), and therefore strongly influence AS properties. This study evaluated the component fractions of EPS normalized to volatile suspended solids (VSS) in waste activated sludge (WAS) from a trickling-filter-solids contact process (TF/SC) and its ability to biosorb organic matter from raw wastewater with 30 min of contact time. Biosorption is the process in which organic matter (carbohydrates, proteins, humic acids, DNA, uronic acids, and lipids) in a sorbate, such as raw wastewater, sorbs onto a sorbent such as WAS. A statistically significant correlation was found between both the total concentration of EPS and the proteins component of the EPS and the biosorption removal of soluble chemical oxygen demand (sCOD) and truly soluble COD (ffCOD). Thus, the biosorption of soluble forms of COD can accurately be predicted by quantifying just the amount of proteins in WAS-associated EPS. No significant correlations were found for the biosorption of colloidal COD (cCOD). WAS biosorbed 45–75 mg L−1 of COD in 30 min. WAS absorbed or stored the proteins fraction of the soluble microbial products (SMP) during the biosorption process. Higher concentrations of humic acids were found in the biosorption process effluent than in the untreated wastewater, which warrants further study. Longer cation exchange resin (CER) extraction times yielded more total EPS from the sludge: 90 ± 9, 158 ± 3, and 316 ± 44 mg g−1 VSS, for 45-min, 4-h, and 24-h extraction times, respectively. Thus, EPS extracted represented only 9%, 15.8%, and 31.6% of the VSS, respectively, raising questions about whether the accurate characterization of EPS can be performed using the typical extraction time of 45 min due to different extraction rates for different components. It was found that the humic acids fraction was extracted much more slowly than the other fractions.


Author(s):  
Silvia Monteiro ◽  
Daniela Rente ◽  
Mónica V. Cunha ◽  
Tiago A. Marques ◽  
Eugénia Cardoso ◽  
...  
Keyword(s):  

2021 ◽  
Vol 10 (47) ◽  
Author(s):  
K. T. Ash ◽  
I. Alamilla ◽  
Y. Li ◽  
D. C. Joyner ◽  
D. E. Williams ◽  
...  

Reported here is a coding-complete genome sequence of a SARS CoV-2 variant obtained from raw wastewater samples at the University of Tennessee—Knoxville campus. This sequence provides insight into SARS CoV-2 variants that circulate on large college campuses but remain mostly undetected.


2021 ◽  
Vol 11 (5) ◽  
pp. 619-626
Author(s):  
Fateh Guemmaz ◽  
Mohamed Tewfik Bouziane ◽  
Ismail Daoud ◽  
Fethi Benbelaïd ◽  
Redouane Rebai

Algeria, like other arid and semi-arid African countries, is confronted by problems related to rapid population growth and water stress (water short-age). This stress fosters raw wastewater reuse in market gardening and hor-ticulture. The advantages of this wastewater consist of their nutrients whose organic materials contribute to soils enrichment with humus, as well as their availability in large quantities. However, raw wastewater reuse entails a po-tential risk of environmental and water resources pollution. The purpose of this work was to evaluate the parasite load of raw wastewater from the city of Biskra’s discharge outfalls (Biskra valley and Z'mor valley), while consider-ing the demographic and socio-economic profile of populations connected to these outfalls. Our results revealed a variety of parasites in the form of hel-minths eggs at the following average concentrations: Ascaris sp. (33.66 eggs/L,), Trichuris sp. (22 eggs/L), Enterobius vermicularis (19.58 eggs/L), Ancy-lostoma sp. (17.41 eggs/L), Nematodirus sp. 17.83 (eggs/L), Hymenolepis nana (21.66 eggs/L), Moniezia expansa (16.5 eggs/L), Taenia sp. (18.5 eggs/L), Fasciola hepatica (0.58 eggs/L) and Strongyloides spp (17.11 eggs/L). The parasitic helminths eggs distribution varies according to the size of urban areas and the socio-economic level of populations connected to each outfall. This study also highlighted the qualitative and quantitative seasonal varia-tions of helminths eggs in wastewater from Biskra’s two outfalls.


2021 ◽  
Author(s):  
Sooyeol Kim ◽  
Lauren Kennedy ◽  
Marlene Wolfe ◽  
Craig Criddle ◽  
Dorothea Duong ◽  
...  

Wastewater-based epidemiology has gained attention throughout the world for detection of SARS-CoV-2 RNA in wastewater to supplement clinical testing. Methods have been developed using both the liquid and the solid fraction of wastewater, with some studies reporting higher concentrations in solids. To investigate this relationship further, we collaborated with six other laboratories to conduct a study across five publicly owned treatment works (POTWs) where both primary solids and raw wastewater influent samples were collected and quantified for SARS-CoV-2 RNA. Solids and influent samples were processed by participating laboratories using their respective methods and retrospectively paired based on date of collection. SARS-CoV-2 RNA concentrations by mass (gene copies per gram) were higher in solids than in influent by approximately three orders of magnitude. Concentrations in matched solids and influent were positively and significantly correlated at all five POTWs. RNA concentrations in both solids and influent were correlated to COVID-19 incidence rates in the sewershed and thus representative of disease burden; the solids methods appeared to produce a comparable relationship between SARS-CoV-2 RNA concentration measurements and incidence rates across all POTWs. Solids and influent methods showed comparable sensitivity, N gene detection frequency, and calculated empirical incidence rate lower limits. Analysis of solids has the advantage of using less sample volume to achieve similar sensitivity to influent methods.


2021 ◽  
Author(s):  
Peng Liu ◽  
Zihan Li ◽  
Zifan Che ◽  
Hu Xinran ◽  
Ming Ying ◽  
...  

Abstract Enteric viruses are known to be prevalent in municipal wastewater, but information on the health risks arising from wastewater reuse is limited. This study quantified six common enteric viruses in raw wastewater and determined the effectiveness of different secondary and tertiary treatment processes at reducing their abundances in three full-scale wastewater treatment plants in China. In the raw wastewater, polyomavirus BK and norovirus GII (Nov GII) exhibited the highest abundance among the detected DNA and RNA viruses, respectively, with concentrations >5 log10 copies/L. Viruses in the raw wastewater were mainly removed by the secondary treatment processes, with log reduction values ranging from 1 to 2. The tertiary treatment processes of both chlorination and ultraviolet irradiation facilitated the additional reduction of viruses. The quantitative microbial risk assessment was applied to estimate the health risks of adenovirus (Adv) and Nov GII when reusing the treated wastewater for irrigation of public green spaces and crops. Estimated disabled-adjusted life-years of Adv and Nov GII for both reuses were higher than the risk threshold (10−6) required by the WHO in the actual scenarios. More effective treatment technologies should be implemented to remove viruses for safe reuse of the treated wastewater.


2021 ◽  
Author(s):  
Kulyash Meiramkulova ◽  
Timoth Mkilima ◽  
Aliya Темirbekova ◽  
Elmira Bukenova ◽  
Abdilda Meirbekov ◽  
...  

Direct utilization of treated effluent from natural treatment systems for irrigation can be challenging on sensitive plants due to high levels of salinity. Post-treatment of such an effluent prior to its applicability in irrigation can be of significant importance. In this study, the wastewater from a natural treatment plant was treated using a lab-scale filtration system with zeolite as a filter material. Three different column depths (0.5 m, 0.75 m, and 1 m) were used to investigate the effect of column depth on the treatment efficiency of the media. The suitability of the raw wastewater and the treated effluent from each column for irrigation purposes was investigated. The water quality parameters investigated were; electrical conductivity (EC), total dissolved solids (TDS), sodium (Na+), calcium (Ca2+), and magnesium (Mg2+). From the analysis results, it was observed that the column depth had a significant influence on the removal efficiency of the pollutants. Where the removal efficiency was observed to be increasing with the increase in the column depth. The highest removal efficiency (94.58%) was achieved from the combination of electrical conductivity and 1 m column depth, while the lowest removal efficiency (10.05%) was observed from the combination of calcium and 0.5 m column depth. The raw wastewater fell mostly into a “very high” hazard, which is class four (C4) based on electrical conductivity and class four (S4) based sodium adsorption ratio; making it unsuitable for irrigation purposes. However, the status improved after the treatment using different column depths.


FEMS Microbes ◽  
2021 ◽  
Author(s):  
Haya Al-Duroobi ◽  
Sina V Moghadam ◽  
Duc C Phan ◽  
Arash Jafarzadeh ◽  
Akanksha Matta ◽  
...  

Abstract The purpose of this study was to conduct a preliminary assessment of the levels of SARS-CoV-2 RNA in wastewater at the Salitrillo Wastewater Treatment Plant in Texas during the initial peak of COVID-19 outbreak. Raw wastewater influent (24 h composite, time-based 1 L samples, n = 13) was collected weekly during June-August 2020. We measured SARS-CoV-2 RNA in wastewater by reverse transcription droplet digital PCR (RT-ddPCR) using the same N1 and N2 primer sets employed in COVID-19 clinical testing. Virus RNA copies for positive samples (77%) ranged from 1.4×102 to 4.1×104 copies per liter of wastewater, and exhibited both increasing and decreasing trends, which corresponded well with the COVID-19 weekly infection rate (N1: ρ = 0.558, P = 0.048; and N2: ρ = 0.487, P = 0.092). A sharp increase in virus RNA concentrations was observed during July sampling dates, consistent with highest number of COVID-19 cases reported. This could be attributed to an increase in the spread of COVID-19 infection due to the July 4 holiday week gatherings (outdoor gatherings were limited to 100 people during that time). Our data show that wastewater surveillance is an effective tool to determine trends in infectious disease prevalence, and provide complimentary information to clinical testing.


2021 ◽  
Author(s):  
Kevin Calci ◽  
Kevin Calci

This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method describes the collection and transport of raw wastewater samples from a wastewater treatment plant.


2021 ◽  
Vol 11 (18) ◽  
pp. 8638
Author(s):  
Makoto Shigei ◽  
Almoayied Assayed ◽  
Ayat Hazaymeh ◽  
Sahar S. Dalahmeh

Assamra wastewater treatment plant (WWTP) is the largest treatment facility in Jordan. Treated wastewater is discharged into the Zarqa River (ZR) and used to irrigate fodder and vegetables. ZR also includes surface runoff, stormwater, and raw wastewater illegally discharged into the river. This study examined pharmaceutically active compounds (PhAC) in water resources in the ZR basin. Samples of WWTP influent and effluent and river water from four sites along ZR were collected. Concentrations of 18 target antibiotics, one stimulant, and 15 other PhACs were determined in the samples. Five antibiotics were detected in WWTP influent (510–860 ng L−1 for ∑Antibiotics) and six in the effluent (2300–2600 ng L−1 for ∑Antibiotics). Concentrations in the effluent of all antibiotics except clarithromycin increased by 2- to 5-fold compared with those in influent, while clarithromycin concentration decreased by around 4- fold (from 308 to 82 ng L−1). WWTP influent and effluent samples contained 14 non-antibiotic PhACs, one simulant, and six antibiotics at detectable concentrations. The dominant PhACs were paracetamol (74% of ∑PhACs) in the influent and carbamazepine (78% of ∑PhACs) in the effluent. At ZR sampling sites, carbamazepine was the dominant PhAC in all cases (800–2700 ng L−1). The antibiotics detected in WWTP effluent were also detected at the ZR sites. In summary, water in ZR is contaminated with PhACs, including antibiotics, and wastewater discharge seems to be the main pathway for this contamination. The occurrence of antibiotics and other PhACs in the irrigated soil requires investigation to assess their fate.


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