Biospeckle Activity of Highbush Blueberry Fruits Infested by Spotted Wing Drosophila (Drosophila suzukii Matsumura)

In this study, the potential of the biospeckle phenomenon for detecting fruit infestation by Drosophila suzukii was examined. We tested both graphical and analytical approaches to evaluate biospeckle activity of healthy and infested fruits. As a result of testing the qualitative approach, a generalized difference method proved to be better at identifying infested areas than Fujii’s method. Biospeckle activity of healthy fruits was low and increased with infestation development. It was found that the biospeckle activity index calculated from spatial-temporal speckle correlation of THSP was the best discriminant of healthy fruits and fruits in two different stages of infestation development irrespective of window size and pixel selection strategy adopted to create the THSP. Other numerical indicators of biospeckle activity (inertia moment, absolute value of differences, average differences) distinguished only fruits in later stage of infestation. Regular values of differences turned out to be of no use in detecting infested fruits. We found that to provide a good representation of activity it was necessary to use a strategy aimed at random selection of pixels gathered around the global maximum of biospeckle activity localized on the graphical outcome. The potential of biospeckle analysis for identification of highbush blueberry fruits infested by D. suzukii was confirmed.

Numerical model for evaluating the speckle activity and characteristics of bone tissue under the biospeckle laser system

This paper discusses and studies the composition and characteristics of biospeckle on the surface of bone tissues. We used a laser speckle device to capture biospeckle patterns from fresh pig bone tissue. Traditional speckle activity metrics were used to measure the speckle activity of ex vivo bone tissue over time. Both Gaussian and Lorentzian correlation functions were used to characterize the ordered and disordered motion of the bone surface, together with volume scattering, to construct the model. Using the established mathematical model of the spatio-temporal evolution of the biospeckle pattern, it is possible to account for the presence of volume scattering from the biospeckle of bones, quantify the ordered or disordered motions in the biological speckle activity at the current time, and assess the ability of laser speckle correlation technique to determine biological activity.