Effect of forage Legetan (Synedrella nodiflora) fresh and hay on the physical quality of forage pellets

This study aimed to determine the effect of raw materials Legetan in the form of fresh and hay on the physical quality of pellets. Legetan (Synedrella nodiflora) was used as main ingredient, in the form of fresh and hay. The research method used a Randomized Completely Block Design (RCBD). Then, if there was a significant difference, it will be analyzed further using Duncan multiple range test (DMRT). The treatments consisted of P1 = 10% rice bran + 90% fresh Legetan; P2 = 20% rice bran + 80% fresh Legetan; P3 = 10% rice bran + 90% hay Legetan; P4 = 20% rice bran + 80% hay Legetan. The variables measured were rendement, color, texture, and price. The results showed that the utilization of Legetan forage as raw material, both hay and fresh with the proportions of rice bran either 10:90 or 20:80, had no significant effect on the physical quality of Legetan forage pellets. However, the higher the portions of Legetan forage in the forage pellets, the more affordable the pellet price will be.

Studi Kunjungan Harian Arthropoda Pada Tanaman Ageratum Conyzoides dan Synedrella Nodiflora di Area Pertanian Desa Wringinpitu Kecamatan Mojowarno Sebagai Bahan Pengembangan E-Katalog

Refugia merupakan tanaman yang dapat menarik dan mengundang sejumlah musuh alami seperti hama, pretador dan parasitoid. Selain itu, refugia juga membantu para petani untuk melindungi sawahnya seperti tanaman padi, jagung dan lain-lain. Dengan adanya tumbuhan liar atau tanaman refugia sebagai bentuk pelestarian Artrhopoda agar ekosistem tetap terjaga dan seimbang. Penelitian ini bertujuan untuk mengetahui frekuensi dan distribusi temporal kunjungan harian Arthropoda pada tanaman Ageratum Conyzoides dan Synedrella Nodiflora hasilnya dari penelitian tersebut akan digunakan sebagai bahan pengembangan e-katalog. Penelitian dilakukan di area pertanian Desa Wringinpitu Kecamatan Mojowarno Kabupaten Jombang. Metode yang digunakan dalam penelitian ini adalah metode visual control dengan pengamatan dilakukan secara 3x ulangan. Hasil penelitian ini menunjukkan adanya perbedaan frekuensi dan ditribusi temporal kunjungan Arthropoda terhadapan tanaman Ageratum Conyzoides dan Synedrella Nodiflora. Pada tumbuhan liar Ageratum Conyzoides dikunjungi oleh 8 family Arthropoda sedangan tumbuhan liar Synedrella Nodiflora hanya dikunjungi 6 family Arthropoda. Hasil dari kegiatan pengamatan ini akan dibuat sebagai pengembangan bahan ajar e-katalog untuk memudahkan peserta didik agar dapat mengenal dan mempelajari tentang hewan Artrhopoda yang ada di area pertanian. Agar mendapatkan kelayakan dalam buku sampingan peserta didik ini, perlu divalidasi oleh ahli materi dan peserta didik dengan aspek-aspek yang dinilai mengguunakan skala likert kemudian dianalisis dan diperoleh tingkat pencapaian sebesar 80 % yang artinya layak digunakan.

Uji Efektivitas Campuran Herbisida Berbahan Aktif Atrazin dan Topramezon terhadap Beberapa Jenis Gulma

The purpose of this study was to determine the effectiveness of mixing herbicides with the active ingredients atrazine and topramezone in controlling weeds and to determine the nature of the mixture of the two active ingredients. This research was conducted in a plastic house in Natar District, South Lampung Regency from October 2020 - January 2021. The study was arranged in a Completely Randomized Design (CRD). The treatments consisted of three types of herbicides with six dosage levels of the active ingredients, namely the single herbicide Atrazine 300 g/l (0, 37.5, 75, 150, 300, and 600 g ai ha-1), Topramezon 10 g/l (0. 1.25 , 2.5, 5, 10, and 20 g ai ha-1), and the herbicide mixture of Atrazine 300 g/l + Topramezone 10 g/l (0. 38.75, 77.50, 155, 310, and 620 g ai ha-1) , and repeated 6 times. The target weeds included broadleaf weeds (Ageratum conyzoides and Synedrella nodiflora), grass groups (Digitaria ciliaris, Echinochloa colonum, and Eleusine indica), and the puzzle group (Cyperus iria). The herbicides atrazine and topramezone have different ways of working so that the analytical method used is the Multiplicative Survival Model (MSM) method. The results showed that mixing the herbicide Atrazine 300 g/l + Topramezon 10 g/l had an expected LD50 value of 46.28 g ai ha-1 and a treatment LD50 of 27.22 g ai ha-1 with a co-toxicity value of 1.7 (Co-toxicity > 1) so that it is synergistic.Key words: Atrazin, Topramezon, mixing herbicide, Multiplicative Survival Model, weed, LD50

Screening insectary refugia plants that increase the performance of Diadegma semiclausum Hellen (Hymenoptera: Ichneumonidae) against diamondback moth larvae

Asmoro PP, Dadang, Pudjianto, Winasa IW. 2021. Screening insectary refugia plants that increase the performance of Diadegma semiclausum Hellen (Hymenoptera: Ichneumonidae) against diamondback moth larvae. Biodiversitas 22: 4254-4260. Habitat manipulation through the planting of insectary refugia plants is one way of controlling pests. These refugia plants serve as the source of nutrients that improve the fitness of parasitoid against insect pests. In the cabbage agroecosystem, the presence of refugia plants is expected to benefit the parasitoid D. semiclausum. This study aimed to select insectary refugia plants beneficial for the D. semiclausum in term of attracting and increasing their longevity, and parasitization ability against P. xylostella larvae. Ten species of refugia plants were used in this study, i.e., Ageratum conyzoides, Galinsoga parviflora, Sphagneticola trilobata, Synedrella nodiflora, Portulaca oleracea, Rorippa indica, Brassica rapa, Apium graveolens, Ocimum basilicum, and Arachis pintoi. The methods used in this study included testing the response of the imago olfactory to refugia plants and its effect on the longevity and parasitization level. The results showed that of the ten species of investigated, R. indica and A. graveolens were the most attractive for the presence of parasitoids. The two refugia plants could also increase the longevity and parasitization rate compared to the water treatment. R. indica and A. graveolens are recommended as refuge plants in the cabbage agroecosystems to control P. xylostella.

Local adaptation of invasive plant, Synedrella nodiflora, in urban tropical lowland landscape Universitas Indonesia

Synedrella nodiflora is an invasive species originated from tropical America and now has spread throughout Indonesia. We analysed the ability of Synedrella nodiflora from the level of HSP70 gene expression at different heat stress in urban tropical lowland landscape Universitas Indonesia. We used the qPCR to quantify the level of HSP70 gene expression and analysed using Pfaffl model. We found the level of HSP70 gene expression got higher related to elevated temperature from 29oC to 39oC with a range of fold from 123.1 to 1676.9. This ability reflects the adaptive plasticity of Synedrella nodiflora in the course of the invasion process.

In Vivo Antimalarial Activity and Toxicity Study of Extracts of Tagetes erecta L. and Synedrella nodiflora (L.) Gaertn. from the Asteraceae Family

Objective. To investigate the antimalarial effects and toxicity of the extracts of the flowers of Tagetes erecta L. and the leaves of Synedrella nodiflora (L.) Gaertn. in a mouse model. Methods. To determine the in vivo antimalarial activity of the extracts, mice were intraperitoneally injected with the Plasmodium berghei ANKA strain and then administered T. erecta or S. nodiflora extract daily for 4 days. Parasitemia was observed by light microscopy. For the detection of acute toxicity, the mice received a single dose of T. erecta or S. nodiflora extract and were observed for 14 days. Biochemical parameters of liver and kidney function and the histopathology of liver and kidney tissues of the acute toxicity group were then examined. Results. T. erecta and S. nodiflora crude extracts at a dose of 600 mg/kg body weight significantly suppressed parasitemia in malaria-infected mice by 65.65% and 62.65%, respectively. Mice treated with 400 mg/kg T. erecta and S. nodiflora crude extracts showed 50.82% and 57.67% suppression, and mice treated with 200 mg/kg displayed 26.33% and 38.57% suppression, respectively. Additionally, no symptoms of acute toxicity were observed in the T. erecta- and S. nodiflora-treated groups. Moreover, no significant alterations in the biochemical parameters of liver and kidney function and no histological changes in the liver or kidney tissues were observed. Conclusions. This study revealed that both T. erecta and S. nodiflora extracts have antimalarial properties in vivo with less toxic effects. Further studies are needed to elucidate the mechanisms of the active compounds from both plants.

Synedrella nodiflora Extract Depresses Excitatory Synaptic Transmission and Chemically-Induced In Vitro Seizures in the Rat Hippocampus

Extracts of the tropical Cinderella plant Synedrella nodiflora are used traditionally to manage convulsive conditions in the West African sub-region. This study sought to determine the neuronal basis of the effectiveness of these plant extracts to suppress seizure activity. Using the hippocampal slice preparation from rats, the ability of the extract to depress excitatory synaptic transmission and in vitro seizure activity were investigated. Bath perfusion of the hydro-ethanolic extract of Synedrella nodiflora (SNE) caused a concentration-dependent depression of evoked field excitatory postsynaptic potentials (fEPSPs) recorded extracellularly in the CA1 region of the hippocampus with maximal depression of about 80% and an estimated IC50 of 0.06 mg/ml. The SNE-induced fEPSP depression was accompanied by an increase in paired pulse facilitation. The fEPSP depression only recovered partially after 20 min washing out. The effect of SNE was not stimulus dependent as it was present even in the absence of synaptic stimulation. Furthermore, it did not show desensitization as repeat application after 10 min washout produced the same level of fEPSP depression as the first application. The SNE effect on fEPSPs was not via adenosine release as it was neither blocked nor reversed by 8-CPT, an adenosine A1 receptor antagonist. In addition, SNE depressed in vitro seizures induced by zero Mg2+ and high K+ -containing artificial cerebrospinal fluid (aCSF) in a concentration-dependent manner. The results show that SNE depresses fEPSPs and spontaneous bursting activity in hippocampal neurons that may underlie its ability to abort convulsive activity in persons with epilepsy.

GLANDULAR TRICHOME IN THE ASTERACEAE FAMILY

The Asteraceae is a diverse plant species and widely distributed, especially in the tropics and subtropics, consisting of 1,600 - 1,700 genera which include 24,000 - 30,000 species. Asteraceae has characteristics of cup flowers and brackets that are not owned by other plants. The objective of this study was to determine the morphological structure of plants and escpecially the leaf glandular trichome in several species of Asteraceae. The research method used was a descriptive method, to describe and interpret the shape, structure and distribution of leaf trichome in the Asteraceae family. This study used eight species of the Asteraceae family, namely Elephantopus mollis, Bidens pilosa, Tithonia deversifolia, Tridax procumbens, Synedrella nodiflora, Eclipta prostrate, Sphagneticola trilobata and Ageratum conyzoides. The observation results of trichomes at 8 species by Scanning Electrone Microscope (SEM) was obtained varied forms of trichomes, both in shape and size. From the research, it was obtained that the forms of multicellular glandular trichome with various shapes, ranging in size from 50.6 µm - 831.9 µm.