bovine immunoglobulin
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2021 ◽  
pp. 3211-3215
Author(s):  
Marian Hampe ◽  
Stefanie Söllner-Donat ◽  
Klaus Failing ◽  
Axel Wehrend

Background and Aim: Rapid tests are routinely used to estimate serum immunoglobulin G (IgG) concentrations in diagnosing a failure of passive transfer (FPT) in calves. The study aimed to compare the Fassisi® Bovine IgG (FB-IgG) immunoassay and an enzyme-linked immunosorbent assay for quantifying bovine IgG in neonatal calf serum. Materials and Methods: A total of 277 calves of 1-10 days of age were used in this study. Blood samples were obtained, and serum was extracted by centrifuging the samples at 2740× g for 5 min at 20°C. The serum was analyzed using the FB-IgG according to the manufacturer's specifications. Serum IgG concentrations were also determined by enzyme-linked immunosorbent assay (ELISA-IgG). FPT was defined as a serum IgG concentration <10 mg/mL. Results: The mean ELISA-IgG serum concentration was 8.40 mg/mL (SD=7.02, range=0.10-47.50 mg/mL). FPT prevalence based on the ELISA measurements was 66.8%. The prevalence of partial and full FPT based on the FB-IgG was 54.5%. The ELISA-IgG and FB-IgG results were subjected to correlation and regression analysis. Overall sensitivity and specificity of the FB-IgG were 61.1% and 58.7%, respectively. A statistically significant dependence on age was identified in the results. Conclusion: Our findings suggest that the FB-IgG rapid method is less accurate and provides no other advantages over established methods.


2021 ◽  
Author(s):  
Netanya S. Utay ◽  
David M. Asmuth ◽  
Shahin Gharakhanian ◽  
Moises Contreras ◽  
Christopher D. Warner ◽  
...  

Cureus ◽  
2021 ◽  
Author(s):  
Matthew J Stotts ◽  
Amanda Cheung ◽  
Muhammad B Hammami ◽  
David J Westrich ◽  
Eric Anderson ◽  
...  

2020 ◽  
Vol 21 (23) ◽  
pp. 9077
Author(s):  
Anneke H. Hellinga ◽  
Theodoros Tsallis ◽  
Talitha Eshuis ◽  
Vassilis Triantis ◽  
Laurien H. Ulfman ◽  
...  

Bovine immunoglobulin G (bIgG) was previously shown to enhance innate immune responses to toll-like receptor (TLR) stimulation, via induction of trained immunity. In this study, we investigated whether minimally processed dairy streams with high levels of whey proteins as potential infant nutrition ingredients could also induce trained immunity, and to what extent this can be explained by the presence of bIgG. The minimally processed whey ingredients serum protein concentrate (SPC) and whey protein concentrate (WPC) were tested for their ability to induce trained immunity in human peripheral blood monocytes. Both ingredients induced trained immunity as evidenced by an increased production of TNF-α and, to a lesser extent, of IL-6 upon stimulation with TLR ligands. This was comparable to isolated bovine immunoglobulin G (bIgG) that served as positive control. Depletion of bIgG from both whey protein-containing ingredients did not significantly inhibit the induction of trained immunity, suggesting that the streams contain other components in addition to bIgG that are able to induce trained immunity. These results indicate that minimally processed whey ingredients may contribute to protection against infections through enhancing innate immune responsiveness to pathogens.


2020 ◽  
Author(s):  
Caoimhe Robinson ◽  
Niamh Creedon ◽  
Riona sayers ◽  
emer kennedy ◽  
Alan O'Riordan

Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus,<i> in utero</i>. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate


2020 ◽  
Author(s):  
Caoimhe Robinson ◽  
Niamh Creedon ◽  
Riona sayers ◽  
emer kennedy ◽  
Alan O'Riordan

Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus,<i> in utero</i>. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate


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