Transcriptomics Reveals Host-Dependent Differences of Polysaccharides Biosynthesis in Cynomorium songaricum

Cynomorium songaricum is a root holoparasitic herb that is mainly hosted in the roots of Nitraria roborowskii and Nitraria sibirica distributed in the arid desert and saline-alkaline regions. The stem of C. songaricum is widely used as a traditional Chinese medicine and applied in anti-viral, anti-obesity and anti-diabetes, which largely rely on the bioactive components including: polysaccharides, flavonoids and triterpenes. Although the differences in growth characteristics of C. songaricum between N. roborowskii and N. sibirica have been reported, the difference of the two hosts on growth and polysaccharides biosynthesis in C. songaricum as well as regulation mechanism are not limited. Here, the physiological characteristics and transcriptome of C. songaricum host in N. roborowskii (CR) and N. sibirica (CS) were conducted. The results showed that the fresh weight, soluble sugar content and antioxidant capacity on a per stem basis exhibited a 3.3-, 3.0- and 2.1-fold increase in CR compared to CS. A total of 16,921 differentially expressed genes (DEGs) were observed in CR versus CS, with 2573 characterized genes, 1725 up-regulated and 848 down-regulated. Based on biological functions, 50 DEGs were associated with polysaccharides and starch metabolism as well as their transport. The expression levels of the selected 37 genes were validated by qRT-PCR and almost consistent with their Reads Per kb per Million values. These findings would provide useful references for improving the yield and quality of C. songaricum.

Wind Tunnel Test on Windblown Sand Two-Phase Flow Characteristics in Arid Desert Regions

Windblown sand two-phase flow characteristics become an essential factor in evaluating the windblown sand load on infrastructures and civil structures. Based on the measured wind characteristics in arid desert regions, windblown sand flow fields with three kinds of sand beds are simulated in the wind tunnel, respectively. The results indicate that the characteristic saltation height of sand particles increases with the wind speed and particle size in the windblown sand flow field. As the sand concentration increases, the wind speed decreases, and the turbulence intensity increases. The concentration, energy, and impact pressure of sand particles increase with increasing wind speed and decrease exponentially with increasing height. At the same wind speed, the concentration, energy, and impact pressure of the coarse sand, fine sand, and mixed sand increases, in turn. Moreover, the variation of kinetic energy with height is similar to that of total energy with height and the proportion of potential energy to total energy is quite small.

Antarctolichenia onofrii gen. nov. sp. nov. from Antarctic Endolithic Communities Untangles the Evolution of Rock-Inhabiting and Lichenized Fungi in Arthoniomycetes

Microbial endolithic communities are the main and most widespread life forms in the coldest and hyper-arid desert of the McMurdo Dry Valleys and other ice-free areas across Victoria Land, Antarctica. There, the lichen-dominated communities are complex and self-supporting assemblages of phototrophic and heterotrophic microorganisms, including bacteria, chlorophytes, and both free-living and lichen-forming fungi living at the edge of their physiological adaptability. In particular, among the free-living fungi, microcolonial, melanized, and anamorphic species are highly recurrent, while a few species were sometimes found to be associated with algae. One of these fungi is of paramount importance for its peculiar traits, i.e., a yeast-like habitus, co-growing with algae and being difficult to propagate in pure culture. In the present study, this taxon is herein described as the new genus Antarctolichenia and its type species is A. onofrii, which represents a transitional group between the free-living and symbiotic lifestyle in Arthoniomycetes. The phylogenetic placement of Antarctolichenia was studied using three rDNA molecular markers and morphological characters were described. In this study, we also reappraise the evolution and the connections linking the lichen-forming and rock-inhabiting lifestyles in the basal lineages of Arthoniomycetes (i.e., Lichenostigmatales) and Dothideomycetes.

Systematical Evaluation of Three Gridded Daily Precipitation Products Against Rain Gauge Observations Over Central Asia

Understanding the precipitation variability and extreme precipitation over arid Central Asia (CA) has largely been hampered by the lack of daily precipitation observations. The gridded precipitation datasets over CA are large discrepancies. Here, three gauge-based gridded daily precipitation products from Asian Precipitation Highly-Resolved Observational Data Integration Towards Evaluation (APHRODITE), Global Precipitation Climatology Center (GPCC), and Climate Prediction Center Based Analysis of Global Daily Precipitation (CPC_global) were assessed and compared with 49 rain gauge daily observations precipitation (OBS) from January 1985 to December 2015 using different time-scales over CA and different climate regimes, specifically Northern CA with temperate continental climate (NCA), Southwestern CA with dry arid desert climate (SWCA), and Southeastern CA with Mediterranean continental climate (SECA). Four accuracy indices [correlation coefficient (R), Bias, root mean square error (RMSE), and relative bias (RBias)] were employed to evaluate the performance of the three products in depicting the spatiotemporal features of precipitation variation over CA at multiple time scales (including daily, monthly, seasonal, and yearly). The mean annual and daily precipitation of OBS and three gridded products exhibit the trend of a gradual precipitation decreased from SECA to NCA and SWCA. The best overall performance was obtained for APHRODITE and GPCC for daily and annual time-scale, whereas CPC shows noticeable underestimation precipitation in SECA. The monthly precipitation depicted distinct features with a bimodal pattern with a peak in March and another in December, include the SECA and SWCA regions. In contrast, precipitation was concentrated in summer with the peak in July over the NCA region. At monthly scale terms, APHRODITE was more accurate in the wet seasons (winter and spring months) in SWCA and SECA. Additionally, GPCC has fairly better capability in summer months in NCA. Considering the spatial distribution, the bias variability was largerly in mountainous areas than in the plains. Temporally, the bias largerly in the dry seasons than in the wet seasons. At the interannual variability scale, GPCC was capable of qualitatively increasing the CA (NCA and SECA) precipitation during the last 21 years, while APHRODITE underestimated the trends. The CPC overestimated the precipitation trends over all regions. This study can serve as a reference for selecting daily precipitation products with low densities of stations, complex topographies, and similar climatic regions.