Comparison of Gradient-Based Edge Detectors Applied on Mammograms

Breast cancer is one of the most common types of cancer amongst women, but it is also one of the most frequently cured cancers. Because of this, early detection is crucial, and this can be done through mammography screening. With the increasing need of an automated interpretation system, a lot of methods have been proposed so far and, regardless of the algorithms, they all share a step: pre-processing. That is, identifying the image orientation, detecting the breast and eliminating irrelevant parts. This paper aims to describe, analyze, compare and evaluate six of the most commonly used edge detection operators: Sobel, Roberts Cross, Prewitt, Farid and Simoncelli, Scharr and Canny. We detail the algorithms, their implementations and the metrics used for evaluation and continue by comparing the operators both visually and numerically, finally concluding that Canny best suit our needs.

Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants

The COVID-19 pandemic highlights the need for diagnostics that can be rapidly adapted and deployed in a variety of settings. Several SARS-CoV-2 variants have shown worrisome effects on vaccine and treatment efficacy, but no current point-of-care (POC) testing modality allows their specific identification. We have developed miSHERLOCK, a low-cost, CRISPR-based POC diagnostic platform that takes unprocessed patient saliva; extracts, purifies, and concentrates viral RNA; performs amplification and detection reactions; and provides fluorescent visual output with only three user actions and 1 hour from sample input to answer out. miSHERLOCK achieves highly sensitive multiplexed detection of SARS-CoV-2 and mutations associated with variants B.1.1.7, B.1.351, and P.1. Our modular system enables easy exchange of assays to address diverse user needs and can be rapidly reconfigured to detect different viruses and variants of concern. An adjunctive smartphone application enables output quantification, automated interpretation, and the possibility of remote, distributed result reporting.

Human–Machine Perception of Complex Signal Data

Electrocardiograms (ECGs), which capture the electrical activity of the human heart, are widely used in clinical practice, and notoriously difficult to interpret. Whilst there have been attempts to automate their interpretation for several decades, human reading of the data presented visually remains the ‘gold standard’. We demonstrate how a visualisation technique that significantly improves human interpretation of ECG data can be used as a basis for an automated interpretation algorithm that is more accurate than current signal processing techniques, and has the benefit of the human and machine sharing the same representation of the data. We discuss the potential of the approach, in terms of its accuracy and acceptability in clinical practice.

Adaptable Automated Interpretation of Rapid Diagnostic Tests Using Few-Shot Learning

Point-of-care lateral flow assays (LFAs) are becomingly increasingly prevalent for diagnosing individual patient disease status and surveying population disease prevalence in a timely, scalable, and cost-effective manner, but a central challenge is to assure correct assay operation and results interpretation as the assays are manually performed in decentralized settings. A smartphone-based software can automate interpretation of an LFA kit, but such algorithms typically require a very large number of images of assays tested with validated specimens, which is challenging to collect for different assay kits, especially for those released during a pandemic. Here, we present an approach - AutoAdapt LFA - that uses few-shot learning, an approach used in other applications such as computer vision and robotics, for accurate and automated interpretation of LFA kits that requires a small number of validated images for training. The approach consists of three components: extraction of membrane and zone areas from an image of the LFA kit, a self-supervised encoder that employs a feature extractor trained with edge-filtered patterns, and few-shot adaptation that enables generalization to new kits using limited validated images. From a base model pre-trained on a commercial LFA kit, we demonstrated the ability of adapted models to interpret results from five new COVID-19 LFA kits (three detecting antigens for diagnosing active infection, and two detecting antibodies for diagnosing past infection). Specifically, using just 10 to 20 images of each new kit, we achieved accuracies of 99% to 100% for each kit. The server-hosted algorithm has an execution time of approximately 4 seconds, which can potentially enable quality assurance and linkage to care for users operating new LFAs in decentralized settings.