The Effect of Efficacy Local Isolates of Paenibacillus Bacteria on Available Soil Phosphorus and Growth of Cultivars Wheat (Triticum Aestivum L.)

A field experiment was carried out to study the effect of bio-inoculation with three local isolates of P. polymyxa and three Varieties of wheat on the growth and yield of the wheat plant (Triticum aestivum L). In the second agricultural research station affiliated to the College of Agriculture - University of Al-Muthanna, a field experiment was conducted in Al-Muthanna Governorate for the year 2020 in silty clay Loam soil texture. The experiment was designed in a randomized complete block method (RCBD) and with three replications. The experiment included two factors, where the first factor represents the local isolates of four levels, symbolized by P0-P1-P2-P3, and the second factor represents the three wheat varieties, Buhouth class 22, Iraq class, and Ibaa 99, which symbolized C1-C2-C3 respectively. The results showed the following: First: The P3 bio-pollination treatment achieved a significant increase in soil phosphorus, potassium, and nitrogen in the available part at the flowering stage, as the highest average was recorded for them (11.39 mg kg −1 soil - 1.4300%-1.4,000%), and the P2 treatment was superior for both soil potassium and plant height. And the dry weight, the highest average for them was recorded (157.8 mg kg −1 - 92.6 cm - 10.61 g plant −1). Second: As for the cultivars, they differed in most of the characteristics of the study. The Iraq cultivar excelled in soil phosphorous concentration with an average of (11.06 mg kg −1 soil), while Buhouth cultivar 22 was superior in soil potassium with an average of (158.02) mg kg −1 soil. Ibaa 99 had been recorded concentration nitrogen and potassium in plants with averages of (1.4208,1.3474%). Third: When the isolates overlapped with the cultivars, a discrepancy was observed. It gave Samawa isolate with Bohouth class 22 by recording the highest average in soil potassium concentration and plant height amounting to (166.7 mg kg −1 soil, 99.8 cm) respectively, while Al-Khidr isolate with Ibaa 99 cultivar in nitrogen and potassium concentration plant, where it reached (1.4567-1.4400)%, while the isolate without addition outperformed with the cultivar Iba 99 for dry weight, as it reached its highest average (11.40 gm plant −1). The isolate of vegetables also outperformed Iraq cultivar for phosphorous concentration in soil, as it recorded the highest average of (12.30) mg kg −1 soil.

Hydrogen Peroxide Can Be a Plausible Biomarker in Cyanobacterial Bloom Treatment

The effect of combined stresses, photoinhibition and nutrient depletion, on the oxidative stress of cyanobacteria was measured in laboratory experiments, to develop the biomass prediction model. Phormidium ambiguum was exposed to various photosynthetically active radiation (PAR) intensities and phosphorous concentrations with fixed nitrogen concentration. The samples were subjected to stress assays by detecting hydrogen peroxide (H2O2) concentration and antioxidant activities of catalase (CAT) and superoxide dismutase (SOD). H2O2 concentration decreased to 30 µmolm-2s-1 of PAR, then increased further with higher PAR intensity. Regarding phosphorus concentration, H2O2 concentration generally decreased with increasing phosphorus concentration. SOD and CAT activities were proportionate to the H2O2 protein-1. No H2O2 concentration detected outside of cells indicated the biological production of H2O2, and the accumulated H2O2 concentration inside cells was parameterized with H2O2 concentration protein-1. Over 30 µmolm-2s-1 of PAR, H2O2 concentration protein-1 had a similar increasing trend with PAR intensity, independently of phosphorous concentration. Meanwhile, with increasing phosphorous concentration, H2O2 protein-1 decreased in a similar pattern regardless of PAR intensity. Protein content decreased with increasing H2O2 gradually up to 4nmol H2O2 mg-1protein, which provides a threshold to restrict the growth of cyanobacteria. With these results. an empirical formula was developed to obtain the cyanobacteria biomass.

A New Approach to Quantifying Bioaccumulation of Elements in Biological Processes

Bioaccumulation, expressed as the bioaccumulation factor (BAF), is a phenomenon widely investigated in the natural environment and at laboratory scale. However, the BAF is more suitable for ecological studies, while in small-scale experiments it has limitations, which are discussed in this article. We propose a new indicator, the bioaccumulation index (BAI). The BAI takes into account the initial load of test elements, which are added to the experimental system together with the biomass of the organism. This offers the opportunity to explore the phenomena related to the bioaccumulation and, contrary to the BAF, can also reveal the dilution of element concentration in the organism. The BAF can overestimate bioaccumulation, and in an extremal situation, when the dilution of element concentration during organism growth occurs, the BAF may produce completely opposite results to the BAI. In one of the examples presented in this work (Tschirner and Simon, 2015), the concentration of phosphorous in fly larvae was lower after the experiment than in the younger larvae before the experiment. Because the phosphorous concentration in the feed was low, the BAF indicated a high bioaccumulation of this element (BAF = 14.85). In contrast, the BAI showed element dilution, which is a more realistic situation (BAI = −0.32). By taking more data into account, the BAI seems to be more valid in determining bioaccumulation, especially in the context of entomoremediation research.

Fixed-bed Column Dynamics of Ultrasound and Na-functionalized Diatomite to Remove Phosphate from Water

A continuous fixed-bed column study has been used to evaluate phosphate adsorption performance of U-D-Na which was functionalized by the cheap NaCl reagent after simple ultrasonic purification of diatomite. Experimentally, various effect factors, the flow rate, the initial phosphate concentration, and the bed height on breakthrough time of fixed column were studied. Experimental results showed that the breakthrough time declined with the increase of inlet phosphorous concentration and feed rate, whereas the increase of bed height turned out to significantly prolong the breakthrough time. The dynamic adsorption data could better be fitted by the Thomas model, with the correlation coefficients obtained, R2 > 0.9000 at the majority of operating conditions (5/7). At least thrice loop of adsorption and desorption was achieved with 0.1 M hydrochloric acid eluent and deionized water. The results proved that U-D-Na could be used as a better alternative phosphate adsorbent from wastewater in a continuous column process.

Determination of phosphorus in different types of waste using the ICP-MS technique

The research aimed to provide an optimized method for the determination of phosphorous concentration in different types of solid waste using inductive coupled plasma mass spectrometry (ICP-MS) technique. The analyzed waste matrices are: a) vegetable waste (P1), b) ash from the incineration of medical waste (P2) c) sewage sludge (P3) and d) sludge from the meat processing industry (P4). The results obtained by the ICP-MS method were compared with the UV-VIS spectrophotometric method for the determination of total phosphorus. In the case of using the colorimetric method by UV-VIS technique, lower results were obtained compared to the ICP-MS method due to the interferences given by the reagents used to determine the total phosphorus. The interferences given by other elements (metals) were also investigated and the performance parameters were determined such as detection limit, quantification limit, recovery and expandend incertainty using ICP-MS technique.

Adjustment of phosphorus concentration to increase growth and yield of cherry tomato using hydroponic drip system

The phosphorus element plays an essential role in plant growth both at the vegetative and generative phases, so its concentration modification in the nutrient solution is necessary to stimulate vegetative growth and crop yield. The research aimed to know the influence of different phosphorus concentrations on growth and yield of cherry tomato using a hydroponic drip irrigation system, conducted from February to June 2017 at Green House Research Station of Universitas Padjajaran Jatinangor using Completely Randomized Design with five treatments and five replications. The treatments were: phosphorous concentration of 100 ppm (P/N ratio 0.4), 125 ppm (P/N ratio 0.5), 150 ppm (P/N ratio 0.6), 175 ppm (P/N ratio 0.7), and 200 ppm (P/N ratio 0.8). The results showed that the increasing concentration of phosphorus improved crops height at the end of the vegetative phase, increased the number of flowers from the beginning to the end of the generative period, was able to prevent the flower fall, enhanced harvest index and weight of tomato fruit significantly at harvest time. Application of 200 ppm phosphorus concentration can increase growth and yield of cherry tomato. Unsur fosfor berperan penting dalam pertumbuhan tanaman pada fase vegetatif maupun fase generatif. Konsentrasi unsur P pada nutrisi tanaman sangat penting untuk merangsang pertumbuhan vegetatif dan hasil panen. Penelitian ini bertujuan untuk mengetahui pengaruh ragam kosentrasi unsur posfor terhadap pertumbuhan dan hasil tanaman tomat cherry pada sistem hidroponik irigasi tetes. Penelitian telah dilaksanakan pada bulan Februari sampai Juni 2017 di Green House Universitas Padjadjaran Jatinangor menggunakan rancangan acak lengkap terdiri dari lima perlakuan dan lima ulangan. Perlakuan konsentrasi posfor, yaitu 100 ppm (rasio P/N 0,4), 125 ppm (rasio P/N 0,5), 150 ppm (rasio P/N 0,6); 175 ppm (rasio P/N 0,7), dan 200 ppm (rasio P/N 0,8). Hasil penelitian menunjukkan bahwa peningkatan konsentrasi posfor meningkatkan tinggi tanaman pada akhir fase vegetatif, meningkatkan jumlah bunga dari awal sampai akhir fase generatif, mengurangi jumlah bunga gugur, meningkatkan indeks panen dan berat buah. Aplikasi konsentrasi posfor 200 ppm dapat meningkatkan pertumbuhan dan hasil tanaman tomat cherry.

Effectiveness prediction of Evogliptin treatment in type 2 diabetes mellitus in russian-korean population

Background: Individualized treatment has already become a part of a routine clinical care. Many data on the effectiveness prediction of commercially available DPP-4 inhibitors had been published, but not on the effectiveness prediction of evogliptin. Aim: To reveal the clinical characteristics and metabolic predictors of better hypoglycemic response to evogliptin. Matherials and methods: We have conducted a retrospective study, based on the data of a randomized clinical trial comparing effectiveness and safety of evogliptin and sitagliptin in Russian and Korean subpopulations. We provide univariate linear regression models for separate subpopulations and a multivariate stepwise regression model for the combined subpopulation. HbA1c change after 24 weeks of evogliptin treatment was a primary endpoint and a dependent variable in the analysis. Results: The decrease of HbA1c after 24 weeks of treatment with evogliptin in Russian subpopulation negatively correlates with triglycerides/HDL level (p = 0,046). In South Korean subpopulation it correlates positively with HbA1c level at baseline (p 0,0001). In order to increase the statistical power of the analysis the data of both populations were combined. According to the combined data, the decrease of HbA1c after 24 weeks of treatment with evogliptin positively correlates with HbA1c level at baseline (p0.0001) and log(HOMA-B) (p=0.0042), and it negatively correlates with log(triglycerides/HDL) (p=0.0057), blood phosphorous concentration (p=0.014) and statin treatment (p=0.044). No correlation of HbA1c change at week 24 was observed with body mass index, diabetes duration and blood C-peptide concentration. Patients able to achieve HbA1c7,0 % had higher HOMA-B (53.22 36.95 и 39.67 24.74, respectively, р=0.033) and were tend to have higher HDL concentration (1.36 0.28 и 1.26 0.26 mmol/l, respectively, р=0.076) and lower triglycerides to HDL ratio (0.87 0.70 и 1.48 0.95, respectively, р=0.079). Conclusion: A patient, who benefits more when treated with evogliptin, has higher HOMA-B, lower triglycerides to HDL ratio and phosphorous concentration in the 1-2 quartiles of the normal range. Triglycerides to HDL ratio is, probably, a specific effectiveness predictor for Russian, but not for Korean subpopulation. These data prove the difference in effectiveness prediction for different drugs of DPP-4 inhibitors group and reveal the need of further investigation.

Fire hazard of phosphorus-containing hard casting polyurethane foams

Introduction. Fire-safe rigid filling polyurethane foams (PUF), meant for low-temperature thermal insulation of pipelines and technological equipment were developed. The effect of concentration of oxyethylated tetraalkylphosphonate pentaerythritol (phostetrol-1) on technological, physico-mechanical and thermal properties, contents of pyrolysis products and main fire hazard indicators of PUFs was explored. The effect of chemical nature and metal compounds concentration of variable valence on fume-generation ability of phosphorous-containing PUFs was examined. Main technological and physico-mechanical properties and fire hazard indicators of developed styrofoams are provided. Materials and methods. Rigid filling PUFs were obtained on the basis of simple oxyethylated polyols and polyisocyanate. Phostetrol-1 was used as a reactive phosphorous-containing compound. As a foaming and hardening catalyst of developed PUF’s a nitrogen-containing polyol (mark Lapromol 294) and dimethylethanolamine was used, and as a foaming agent - mixture of freon - 11 and water. Different metal compounds of variable valence were used to reduce fume-generation ability and toxicity of pyrolysis products of rigid phosphorous-containing PUFs. Thermal properties of examined PUFs were studied with the help of thermoanalytical complex Du PONT 9000. Main technological and physico-mechanical properties and fire hazard indicators of styrofoams were determined under existing GOSTs. Results. The effect of phostetrol-1 concentration in polyester compound on main technological and physico-mechanical properties, contents of pyrolysis products and main fire hazard indicators of rigid filling PUFs was established. It is shown that to obtain moderately flammable PUFs the phosphorous concentration in styrofoam must exceed 2.5 mass. %. The correlation between low-scale evaluation methods of flammability of rigid PUFs was found. The effect of phosphorous concentration on fume-generation ability and contents of pyrolysis products of rigid PUFs was found. The effect of chemical nature and metal compounds concentration of variable valence on fume-generation ability of phosphorous-containing PUFs was examined. It is shown that effective decrease of fume-generation ability and toxicity of pyrolysis products of moderately flammable PUFs occurs when Cu2O or chrome spinels are introduced to the polyester compound. Conclusions. As a result of conducted research it was established that the combined use of phostetrol-1, Cu2O and chrome spinels makes it possible to obtain rigid fire-safe PUFs with high physico-mechanical properties. Fire-safe rigid filling PUFs, developed with the use of raw native materials, are recommended to be used for low-temperature thermal insulation of pipelines and technological equipment.