Exploring divalent conjugates of 5-N-acetyl-neuraminic acid as inhibitors of coxsackievirus A24 variant (CVA24v) transduction

Coxsackievirus A24 variant (CVA24v) is responsible for several outbreaks and two pandemics of the highly contagious eye infection acute hemorrhagic conjunctivitis (AHC).

Microbial Pattern and Antibiotic Sensitivity Test Among Eye Infection Patients at Bali Mandara Eye Hospital Bali

Infection disease of the eye usually found in health care facilities and can caused by viral,bacterial, parasite and fungi. Bali Mandara Eye Hospital was special hospital for eye disease in Bali. In this hospital infection disease of the eye dominated by bacterial conjunctivitis, viral conjunctivitis, corneal ulver, and endophtalmitis. This study was a restropective descriptive study using patient who are take eye secret culture in Bali Mandara Eye Hospital and sample were take from 2019 till 2020 It can be seen that was found seven microba and dominated disease was corneal ulcer 26 sample (86%) followed by endophtalmitis 4 sample (14%). The most microba were Pseudomonas aeruginosa. Pseudomonas aeruginosa found resistant in almost antibiotics and sensitive tolevofloxacin, gentamycin dan ceflazidime Keywords: infection, eye, culture, antibiotics

Contact lenses contamination by Acanthamoeba spp. in Upper Egypt

Background Acanthamoeba spp. are one of the free-living amoeba that spread worldwide causing keratitis. Owing to the increase in the use of lenses, whether for medical or cosmetic purposes, the incidence of disease increases every year. Contamination of the lenses with the Acanthamoeba trophozoites or cysts may lead to eye infection and cause sight-threatening keratitis in human. We isolated Acanthamoeba spp. from new lenses, used lenses, and contact lens disinfecting solutions and identified them based on morphological characteristics and molecular test. Methods New and used lenses and contact lens disinfecting solutions were cultured on monogenic media. Light and scanning electron microscope was used to identify Acanthamoeba spp. morphological features. Genotype identification was also evaluated using PCR sequencing of 18S rRNA gene specific primer pair JDP1 and JDP2. Results A hundred samples were examined, 29 (29%) were infected with Acanthamoeba spp. That belonged to two strains of Acanthamoeba (Acanthamoeba 41 and Acanthamoeba 68). 18S rRNA of the Acanthamoeba 41 had 99.69% sequence identity to Acanthamoeba castellanii clone HDU-JUMS-2, whereas Acanthamoeba 68 had 99.74% similar pattern to that of Acanthamoeba sp. isolate T4 clone ac2t4 that are morphologically identified as Acanthamoeba polyphaga. The obtained data revealed that the isolated strains belong to T4 genotype that was evolutionarily similar to strains isolated in Iran. Conclusions Cosmetic lenses and disinfectant solutions are a major transmissible mode for infection. This genotype is common as the cause of Acanthamoeba keratitis. To avoid infection, care must be taken to clean the lenses and their preservative solutions and prevent contamination with the parasite.

PSVIII-15 Effect of Endovac-Beef vaccination on performance, dietary energetics, and carcass characteristics of growing-finishing calf-fed Holstein steers

Objectives were to evaluate the influence of ENDOVAC-Beef® (Endovac Animal Health, Columbia, MO) vaccination on calf-fed Holstein steer growth performance, dietary energetics, and carcass characteristics. Fifty Holstein steer calves were utilized in a 322 d trial. Calves were blocked by initial shrunk body weight (134 ±5 kg) and randomly assigned to treatments within weight groups to 10 pens (5 steers per pen, 5 pens per treatment). Five pen groups received the ENDOVAC-Beef vaccination on d 28 and d 56, and the remaining 5 pens did not receive the ENDOVAC-Beef vaccination. All steers were fed the same steam-flaked corn-based growing-finishing diet. Vaccination did not affect (P ≥ 0.10) live weight, average daily gain, and dry matter intake during the 322 d feeding period. However, vaccination increased (P ≤ 0.04) gain efficiency and estimated dietary net energy values (NE) during the first 112 d on feed. Vaccination did not affect (P ≥ 0.14) gain efficiency or estimated dietary NE during the final 112 to 322 d, or over the entire feeding period (1 to 322 d). Vaccination did not affect (P ≥ 0.31) hot carcass weight, dressing percentage, and marbling score. However, vaccinating calf-fed Holstein steers with ENDOVAC-Beef increased (P ≤ 0.01) longissimus muscle area (LMA; 86.5 vs 76.8 cm2) and retail yield (51.3 vs 50.4); but, tended to increase (P = 0.07) carcass fat thickness. Morbidity and pink eye infection were low and not affect by treatment (P ≥ 0.18). We conclude that while vaccinating calf-fed Holstein steers with ENDOVAC-Beef did not have an overall effect on growth-performance, it increased (13%) LMA and retail carcass yield (1.8%).

Targeting Inflammation Driven by HMGB1 in Bacterial Keratitis—A Review

Pseudomonas (P.) aeruginosa is a Gram-negative bacteria that causes human infectionsinfections. It can cause keratitis, a severe eye infection, that develops quickly and is a major cause of ulceration of the cornea and ocular complications globally. Contact lens wear is the greatest causative reason in developed countries, but in other countries, trauma and predominates. Use of non-human models of the disease are critical and may provide promising alternative argets for therapy to bolster a lack of new antibiotics and increasing antibiotic resistance. In this regard, we have shown promising data after inhibiting high mobility group box 1 (HMGB1), using small interfering RNA (siRNA). Success has also been obtained after other means to inhinit HMGB1 and include: use of HMGB1 Box A (one of three HMGB1 domains), anti-HMGB1 antibody blockage of HMGB1 and/or its receptors, Toll like receptor (TLR) 4, treatment with thrombomodulin (TM) or vasoactive intestinal peptide (VIP) and glycyrrhizin (GLY, a triterpenoid saponin) that directly binds to HMGB1. ReducingHMGB1 levels in P. aeruginosa keratitis appears a viable treatment alternative.

Exploring the Effect of Structure-Based Scaffold Hopping on the Inhibition of Coxsackievirus A24v Transduction by Pentavalent N-Acetylneuraminic Acid Conjugates

Coxsackievirus A24 variant (CVA24v) is the primary causative agent of the highly contagious eye infection designated acute hemorrhagic conjunctivitis (AHC). It is solely responsible for two pandemics and several recurring outbreaks of the disease over the last decades, thus affecting millions of individuals throughout the world. To date, no antiviral agents or vaccines are available for combating this disease, and treatment is mainly supportive. CVA24v utilizes Neu5Ac-containing glycans as attachment receptors facilitating entry into host cells. We have previously reported that pentavalent Neu5Ac conjugates based on a glucose-scaffold inhibit CVA24v infection of human corneal epithelial cells. In this study, we report on the design and synthesis of scaffold-replaced pentavalent Neu5Ac conjugates and their effect on CVA24v cell transduction and the use of cryogenic electron microscopy (cryo-EM) to study the binding of these multivalent conjugates to CVA24v. The results presented here provide insights into the development of Neu5Ac-based inhibitors of CVA24v and, most significantly, the first application of cryo-EM to study the binding of a multivalent ligand to a lectin.

Buffaloes (Bubalus bubalis) mycotic eye infection: reported herd cases in Thi-Qar province, Iraq

Buffaloes are one of the important farm animals in the south of Iraq and play an essential economical role mainly acting as dairy, meat, and draft animals. This study intended to diagnose buffalo mycotic eye infections in Thi-Qar province/Iraq. Some 250 buffaloes in the herd of 3,700 animals suffered from eye infections from December 2017 to November 2018. Eye swabs were collected from each infected eye of the affected buffaloes of both sexes before treatment. The animals were in different age groups. All samples were transferred to the laboratory in transfer media, and cultured on Sabouraud dextrose (SDA) agar with and without 0.05 g/mL and 0.4 g/mL chloramphenicol and cycloheximide, respectively. Later, the agars were incubated at 25o C and 37o C. The total percentage of eye infection was (6.75%), constituting (49.2%) mycotic infections. The predominant clinical manifestations that appeared on the infected buffaloes were eye inflammation represented by congestion, lacrimation, the opacity of cornea and edema, and reduced productivity of the infected animals. Different fungal isolates were identified from the samples including Aspergillus fumigates, Aspergillus flavus, Aspergillus niger, Penicillium spp., Alternaria spp., Fusarium spp., Candida spp., Cladosporium spp., Rhodotorula spp., Mucor spp. and Rhizopus spp. Calves buffaloes below one-year-old were more prone to mycotic infection than one-year-old or more. Additionally, male buffaloes were more susceptible to infection than females. In conclusion, this study isolated various types of fungus from the inflamed eyes of buffaloes. Fungal eye infection and the potential risk factors for fungal keratitis in buffaloes were also investigated. The study also approved the rapid diagnosis of fungi by direct microscopic detection and culture. The author recommends future studies including large numbers of the buffalo herd in Iraq to determine the epidemiology of this condition in the country.

A Review on Mucormycosis with recent pharmacological treatment

Mucormycosis is antifungal eye infection difficult to manage this infection with limited use of diagnostic tool and therapeutic option, but previous literature studied diagnostic strategies and evaluate the potency of antifungal agent as a treatment option. Mucormycosis was difficult to study on imaging studies. Surgery plus antifungal therapy of high dose yields greater survival rates. Mucorales are most widely resisted to the used as an antifungal agent. Amphotericin -B were kept for de-escalation refractory therapy whereas, patient’s intolerant to Amphotericin B. Keywords: Mucormycosis, amphotericin, antifungal agents

An association between water, sanitation, and hygiene (WASH) and prevalence of trachoma in Monze district of Southern Province, Zambia

This study aims to determine the association between water, sanitation, and hygiene, and the prevalence of trachoma in Monze district, Zambia. The overall prevalence of trachoma among residents of Monze district is 2.0% disaggregated as 3.4% for 1–9 age group and 1.1% for ≥10 age group. The findings reveal an association between trachoma eye infection and drinking water source from protected well/spring, and piped water. After adjusting for other variables, there was an association of drinking water from a protected well/spring (AOR 8.343, CI 1.126–61.803), piped water (AOR 4.127, CI 1.088–15.648), and piped water for washing (AOR 0.172, 95% CI 0.031–0.944.439). The presence of a hand wash facility was very low at 2.9% while hand washing agents were even lower at 0.41%. The study concludes that children are at a higher risk of trachoma prevalence. Other WASH aspects, such as adequacy of water, might be more important than the presence of potable water. The prevalence of trachoma in Monze is WASH focused.