Pemberdayaan kelompok tani kedelai melalui penerapan teknologi pengolahan kedelai untuk meningkatkan pendapatan petani di Desa Marga Mulya Kecamatan Rantau Rasau

The aim of the community service activiy is to increase farmers’ knowledge and income in diversiying soybean processed products. This activity was carried out in Marga Mulya village, Rantai Rasau district, Tanjung Jabung Timur regecy which held from June until December 2019. The method used in this activity is giving education to society through the process of learning and implementation participatory (participatory learning and action), stages taken are; counseling on post-harvest technology for soybeans, practicing and implementing of making flour and soybean powder alongside with its processed products and enhancing packaging. Evaluation had done in the beginning of activity, the evaluation criteria are the number of attendance and the percentage of how active the member of farmers group in each stage. The result of this community service activity shows that most (80%) of farmer group partner undestand and able to use soy processing tool and also to create variety of soybean processed product. It shows the increment of PKM business in achieveing diversiying processed soybean products, increaseing of processed production and product resilicence, as well as rising added value of processed soybean products.

RESEARCH ON THE HYDROLYSIS PROCESS OF SOYBEAN POWDER BY ALCALASE TO PRODUCE COMMERCIAL FERTILIZER

Mục đích của nghiên cứu là khảo sát đơn yếu tố tỉ lệ phối trộn thích hợp giữa enzyme (E) và chế phẩm vi sinh vật có ích (EM), tỉ lệ nước bổ sung, nhiệt độ ủ, thời gian thủy phân ảnh hưởng tới quá trình thủy phân khô đậu tương bằng chế phẩm enzyme alcalase. Hàm lượng protein hòa tan trong dịch thủy phân được xác định bằng phương pháp Lowry. Kết quả khảo sát tương ứng là: EM 2% + E 1.5%; 4 (ml/g); 45ºC; 8 h. Trên cơ sở khảo sát các yếu tố ảnh hưởng đến điều kiện thủy phân, chúng tôi nhận thấy tỷ lệ phối trộn giữa enzyme và EM, tỷ lệ nước bổ sung, nhiệt độ ủ, thời gian thủy phân là những yếu tố ảnh hưởng mạnh đến quá trình thủy phân. Bằng phương pháp quy hoạch thực nghiệm Box- Behnken đã tìm được điều kiện tối ưu quá trình thủy phân khô đậu tương là tỷ lệ phối trộn giữa enzyme và EM là EM 2% + E 1.5%, tỉ lệ nước bổ sung là: khô đậu tương: 4.2, nhiệt độ ủ 45ºC, thời gian thủy phân 8.53 giờ. Kết quả thực nghiệm cho kết quả có độ tương thích cao với mô hình.

Purification and characteration of xylanase from Aspegillus oryzae VTCC F187

Xylanase is produced by many bacteria and fungi, among which Aspergillus oryzae is considered as a potential source. In this study, a xylanase was isolated and purified from the crude culture filtrate of Aspergillus oryzae VTCC F187 after 7 days of growth on the optimal culture containing 7% corncob and 5% soybean powder under liquid-state fermentation. After two steps purification process including gel filtration chromatography (Sephadex G-75) incorporating with anion-exchange chromatography (DEAE-sephadex), obtained xylanase was purified with the yield and purity of 24.9% and 3.91 fold, respectively. The molecular mass of the purified xylanase determined by SDS–PAGEwas 32 kDa with a specific activity of 1268.0 U/mg towards 1% (w/v) of birch wood xylan. The xylanase displayed its optimum activity at 60°C, pH 6.5, and the enzyme remained active effectively within pH 3.0–5.0 and at the temperature below 37°C. Some substances were tested at concentration of 2% (v/v) such as β-mercaptoethanol, DMSO, Tween 80 and 10 mM NaN3 slightly decreased xylanase activity and reached over 85%. While EDTA 10 mM and SDS at concentration of 2% inhibited more strongly, xylanase activity was only 77.6% and 56.6% comparing with control one, respectively. The biochemical characteristics suggested that the xylanase has a potential application, including use as a feed enzyme or using hydrolysis to produce environmentally friendly Bio-products.

Intestinal microbiota and functional characteristics of black soldier fly larvae (Hermetia illucens)

Purpose Black soldier fly transforms organic waste into insect protein and fat, which makes it valuable for ecological utilization. This process is associated with the intestinal microbiota. This research was developed to determine the type and functional characteristics of intestinal microbiota present in black soldier fly larvae. Methods In this research, metagenomics has been used to study black soldier fly larvae gut bacteria, which involves the high abundance of the gut microbe advantage bacterium group, the impact, and the physiological functions of the microbiota. Furthermore, intestinal bacteria and their related functions were investigated by bioinformatics analysis to evaluate potential microbial strains that may be used to improve feed utilization efficiency in factory farming. Result The results showed that black soldier fly larvae’s intestine contains more than 11,000 bacteria. The high relative abundance of group W (larvae fed with 75% wheat bran and 25% soybean powder) may promote feed utilization efficiency, whereas high relative abundance of group T microbiota (larvae fed with 75% wheat bran and 25% soybean powder supplemented with 1% tetracycline) may play an important role in black soldier fly larvae survival. Conclusion The gut bacteria in black soldier fly larvae were involved in polysaccharide biosynthesis and metabolism, translation, membrane transport, energy metabolism, cytoskeleton, extracellular structures, inorganic ion transport and metabolism, nucleotide metabolism, and coenzyme transport physiological processes. The 35 significant differential microbes in group W may have a positive impact on feed utilization and physiological process.

Intestinal Microbiota and Functional Characteristics of Black Soldier Fly Larvae (Hermetia Illucens)

Purpose: Black soldier fly transforms organic waste into insect protein and fat, which makes it valuable to ecological utilization. This process is associated with intestinal microbiota. This research was developed to determine the type and functional characteristics of intestinal microbiota present in black soldier fly larvae.Methods: In this research, metagenomics has been used to study black soldier fly larvae gut bacteria, which involves the high abundance of the gut microbes advantage bacterium group, the impact, and physiological functions of the microbiota. Furthermore, intestinal bacteria and their related functions were investigated by bioinformatics analysis to evaluate potential the microbial strains that may be used to improve feed utilization efficiency in factory farming.Result: The results showed that black soldier fly larvae´ intestine contains more than 11,000 bacteria. High relative abundance of group W (larvae fed with 75% wheat bran and 25% soybean powder) may promote feed utilization efficiency, whereas high relative abundance of group T microbiota (larvae fed with 75% wheat bran and 25% soybean powder supplemented with 1% tetracycline) may play an important role in black soldier fly larvae survival.Conclusion: The gut bacteria in black soldier fly larvae were involved in polysaccharide biosynthesis and metabolism, translation, membrane transport, energy metabolism, cytoskeleton, extracellular structures, inorganic ion transport and metabolism, nucleotide metabolism, and coenzyme transport physiological processes. The 35 significant differential microbes in group W may have a positive impact in feed utilization and physiological process.