porcine kobuvirus
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PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0260161
Author(s):  
Lisandru Capai ◽  
Géraldine Piorkowski ◽  
Oscar Maestrini ◽  
François Casabianca ◽  
Shirley Masse ◽  
...  

Many enteric viruses are found in pig farms around the world and can cause death of animals or important production losses for breeders. Among the wide spectrum of enteric viral species, porcine Sapelovirus (PSV), porcine Kobuvirus (PKoV) and porcine Astrovirus (PAstV) are frequently found in pig feces. In this study we investigated sixteen pig farms in Corsica, France, to evaluate the circulation of three enteric viruses (PKoV, PAstV-1 and PSV). In addition to the three viruses studied by RT–qPCR (908 pig feces samples), 26 stool samples were tested using the Next Generation Sequencing method (NGS). Our results showed viral RNA detection rates (i) of 62.0% [58.7–65.1] (n = 563/908) for PSV, (ii) of 44.8% [41.5–48.1] (n = 407/908) for PKoV and (iii) of 8.6% [6.8–10.6] (n = 78/908) for PAstV-1. Significant differences were observed for all three viruses according to age (P-value = 2.4e–13 for PAstV-1; 2.4e–12 for PKoV and 0.005 for PSV). The type of breeding was significantly associated with RNA detection only for PAstV-1 (P-value = 9.6e–6). Among the 26 samples tested with NGS method, consensus sequences corresponding to 10 different species of virus were detected. This study provides first insight on the presence of three common porcine enteric viruses in France. We also showed that they are frequently encountered in pigs born and bred in Corsica, which demonstrates endemic local circulation.


Author(s):  
Shailesh Kumar Patel ◽  
Mamta Pathak ◽  
Alok Singh ◽  
G. Saikumar

Background: The porcine kobuvirus (PKV) is a single-stranded, positive-sense, RNA virus of the family Picornaviridae which composed of small, non-enveloped viruses. The PKV is suspected to cause large outbreaks of diarrhoea and dehydration along with vomition in suckling piglets with a high morbidity rate. Despite of its economical importance very few studies are available on the pathology of PKV. The present study was conducted with the aim to investigate PKV in naturally infected Indian pigs. Methods: A total of 41 intestinal content and tissue samples from dead pigs were collected from post-mortem facility of, ICAR-IVRI during April, 2019 to July 2020. All the carcasses were subjected to detailed necropsy examination and appropriate tissue samples were collected for histopathological examination and molecular investigation. The amplification of 3D region of PKV was carried out via RT-PCR using the specific primers. The Genetic characterization of Indian isolate of the PKV was done on the basis of viral 3D gene of PKV. Result: A total of six out of 41 necropsied piglets were found positive for the PKV. The diarrhoea and dehydration were observed as the main clinical signs. The thickening and clouding of brain meninges, congestion of brain, pneumonia along with catarrhal and haemorrhagic enteritis was observed as common gross lesions. Microscopic lesions observed in brain were mild to moderate perivascular cuffing, congestion of meningeal and cerebral vessels, focal gliosis and neuronophagia. Mild to severe interstitial pneumonia and emphysema was noticed in lungs. Intestinal mucosa revealed severe inflammatory changes along with sloughing of villi. The genetic characterization revealed maximum identity of 91-93% with Indian isolate of PKV MH394282 and Chinese strain of PKV FJ459905. This study reported the first pathological investigation of PKV from Indian pigs and described the molecular findings in detail. Further explorative surveillance along with epidemiological studies are highly warranted to find out the real impact of the PKV on Indian pigs.


2021 ◽  
Author(s):  
Lisandru Capai ◽  
Géraldine Piorkowski ◽  
Oscar Maestrini ◽  
François Casabianca ◽  
Shirley Masse ◽  
...  

AbstractMany enteric viruses are found in pig farms around the world and can cause death of animals or important production losses for breeders. Among the wide spectrum of enteric viral species, porcine Sapelovirus (PSV), porcine Kobuvirus (PKoV) and porcine Astrovirus (PAstV) are frequently found in pig feces. In this study we investigated sixteen pig farms in Corsica, France, to evaluate the circulation of three enteric viruses (PKoV, PAstV-1 and PSV). In addition to the three viruses studied by RT–qPCR (908 pig feces samples), 26 stool samples were tested using the Next Generation Sequencing method (NGS). Our results showed viral RNA detection rates (i) of 62.0% [58.7–65.1] (n = 563/908) for PSV, (ii) of 44.8% [41.5–48.1] (n = 407/908) for PKoV and (iii) of 8.6% [6.8–10.6] (n = 78/908) for PAstV-1. Significant differences were observed for all three viruses according to age (P-value = 2.4e–13 for PAstV-1; 2.4e–12 for PKoV and 0.005 for PSV). The type of breeding was significantly associated with RNA detection only for PAstV-1 (P-value = 9.6e–6). Among the 26 samples tested with NGS method, consensus sequences corresponding to 10 different species of virus were obtained This study provides first insight on the presence of three common porcine enteric viruses in France. We also showed that they are frequently encountered in pigs born and bred in Corsica, which demonstrates endemic local circulation.ImportanceThis study provides important information in the comprehension of the epidemiology of different viruses circulating in swine farms. We have shown the great diversity of viruses that could be present in extensive farms. Moreover, to our knowledge, this is the first detection of these different viruses in France. So far, this study has to be considered as a first step in the study of enteric viruses in Corsican pig farms.


Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 738
Author(s):  
Montserrat-Elemi García-Hernández ◽  
María-Elena Trujillo-Ortega ◽  
Sofía-Lizbeth Alcaraz-Estrada ◽  
Luis Lozano-Aguirre-Beltrán ◽  
Carlos Sandoval-Jaime ◽  
...  

Swine enteric viral infections are responsible for substantial economic losses in the pork industry worldwide. Porcine epidemic diarrhea (PEDV) is one of the main causative agents of diarrhea in lactating pigs, and reports of PEDV coinfection with other enteric viruses highlight the importance of viral interactions for disease presentation and outcomes. Using next-generation sequencing (NGS) and sequence analyses from samples taken from piglets with acute diarrhea, we explored the possible interactions between PEDV and other less reported pathogens. PEDV coinfection with porcine kobuvirus (PKV) was detected in 36.4% (27/74) of samples. Full genomes from porcine coronavirus and kobuvirus were obtained, as was a partial porcine sapovirus genome (PSaV). The phylogenetic results show the clustering of these strains corresponding to the geographical relationship. To our knowledge, this is the first full genome and isolation report for porcine kobuvirus in México, as well as the first phylogenetic analysis for porcine sapovirus in the country. The NGS approach provides a better perspective of circulating viruses and other pathogens in affected production units.


2021 ◽  
Author(s):  
Jihye Shin ◽  
SeEun Choe ◽  
Bang-Hun Hyun ◽  
Dong-Jun An

Abstract The prevalence of porcine kobuvirus (PKoV) and porcine astrovirus (PAstV) in 845 Korean wild boars (KWB) during 2016-2018 were 28.0% and 10.6%, respectively, and co-infection of two viruses showed 5.1%. Phylogenetic tree analysis also revealed that 236 PKoVs from KWB were divided to diverse lineages within Aichivirus C group but the one strain (WKoV16CN-8627) was included the same cluster with bovine kobuvirus (Achivirus B). Eighty-nine PAstVs from KWB was belonged predominantly to lineage PAstV4 and only one strain (WAst17JN-10931) included novel to lineage PAstV2. Two viruses are epidemic more in young (≤ 12 months) than in old pigs (> 12 months).


2020 ◽  
Vol 132 ◽  
pp. 404-406
Author(s):  
Vesna Milićević ◽  
Branislav Kureljušić ◽  
Jelena Maksimović-Zorić ◽  
Božidar Savić ◽  
Ljiljana Spalević ◽  
...  

2017 ◽  
Vol 61 (2) ◽  
pp. 50-55
Author(s):  
H. McFall ◽  
Š. Vilček

AbstractThe objective of this study was to show if porcine kobuvirus 1 (PKV-1) participates in the development of diarrhoea in piglets. The experiments were focused on comparing the occurrence of PKV-1 with the occurrence of rotavirus A (RVA) infection in suckling pigs on Slovak pig farms. A total of 91 rectal swabs of piglets (age < 28 days) were collected from 8 pig farms. RT-PCR was employed to detect PKV-1 through amplification of the 495 bp fragment of the 3D gene using primers KoVF/ KoVR, and RVA was detected through amplification of the 309 bp fragment of the VP6 gene using primers rot3 and rot5. As expected, the detection of RVA in diarrhoeic piglets was 56.8 % (P < 0.01), while only 14.8 % in healthy animals. These results confirm that RVA is one of the main causes of diarrhoea in young piglets. Comparatively, PKV-1 was detected in approximately equal numbers in the same group of both healthy and diarrhoeic pigs, with 74.1 % in healthy animals and 81.1 % in diarrhoeic animals, which was not statistically significant (P < 0.05). The level of co-infection of both viruses was 11.1 % in healthy animals. A portion of 48.6 % (P < 0.01) of diarrhoeic animals were found with RVA and PKV-1 coinfections. The results of this study indicate that while RVA is an enteric virus, PKV-1 cannot confidently be confirmed as an enteric pathogen.


2017 ◽  
Vol 64 (4) ◽  
pp. 1030-1036 ◽  
Author(s):  
S.-L. Zhai ◽  
H. Zhang ◽  
T. Lin ◽  
S.-N. Chen ◽  
X. Zhou ◽  
...  

2017 ◽  
Vol 49 (5) ◽  
pp. 1077-1080 ◽  
Author(s):  
Watchaporn Chuchaona ◽  
Pattara Khamrin ◽  
Arpaporn Yodmeeklin ◽  
Aphisek Kongkaew ◽  
Ratchaya Vachirachewin ◽  
...  

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