The er2 gene resistance against powdery mildew infection is associated with enhanced antioxidative protection and defense gene expression

2019 ◽  
Vol 106 ◽  
pp. 253-262 ◽  
Author(s):  
Sheetal M. Bhosle ◽  
Nitinkumar Marathe ◽  
Ragiba Makandar
Keyword(s):  
Gene Expression ◽  
Powdery Mildew ◽  
Defense Gene Expression ◽  
Defense Gene ◽  
Antioxidative Protection ◽  
Powdery Mildew Infection

scholarly journals Heat Stress Pre-Exposure May Differentially Modulate Plant Defense to Powdery Mildew in a Resistant and Susceptible Barley Genotype

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 776
Author(s):  
Ildikó Schwarczinger ◽  
Judit Kolozsváriné Nagy ◽  
Lóránt Király ◽  
Klára Mészáros ◽  
Judit Bányai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Powdery Mildew ◽  
Plant Defense ◽  
High Temperatures ◽  
Defense Gene Expression ◽  
Defense Gene ◽  
Barley Line ◽  
Prolonged Heat

Heat stress negatively affects barley production and under elevated temperatures defense responses to powdery mildew (Blumeria graminis f. sp. hordei, Bgh) are altered. Previous research has analyzed the effects of short-term (30 s to 2 h) heat stress, however, few data are available on the influence of long-term exposure to heat on powdery mildew infections. We simultaneously assessed the effects of short and long term heat pre-exposure on resistance/susceptibility of barley to Bgh, evaluating powdery mildew infection by analyzing symptoms and Bgh biomass with RT-qPCR in barley plants pre-exposed to high temperatures (28 and 35 °C from 30 s to 5 days). Plant defense gene expression after heat stress pre-exposure and inoculation was also monitored. Our results show that prolonged heat stress (24, 48 and 120 h) further enhanced Bgh susceptibility in a susceptible barley line (MvHV118-17), while a resistant line (MvHV07-17) retained its pathogen resistance. Furthermore, prolonged heat stress significantly repressed the expression of several defense-related genes (BAX inhibitor-1, Pathogenesis related-1b and Respiratory burst oxidase homologue F2) in both resistant and susceptible barley lines. Remarkably, heat-suppressed defense gene expression returned to normal levels only in MvHV07-17, a possible reason why this barley line retains Bgh resistance even at high temperatures.

scholarly journals HOS15 is a transcriptional corepressor of NPR1-mediated gene activation of plant immunity

2020 ◽  
Vol 117 (48) ◽  
pp. 30805-30815
Author(s):  
Mingzhe Shen ◽  
Chae Jin Lim ◽  
Junghoon Park ◽  
Jeong Eun Kim ◽  
Dongwon Baek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ubiquitin Ligase ◽  
Transcriptional Activation ◽  
Plant Immunity ◽  
Gene Activation ◽  
Transcriptional Corepressor ◽  
Defense Gene Expression ◽  
Dynamic Regulation ◽  
Defense Gene ◽  
Transcriptional Corepressors

Transcriptional regulation is a complex and pivotal process in living cells. HOS15 is a transcriptional corepressor. Although transcriptional repressors generally have been associated with inactive genes, increasing evidence indicates that, through poorly understood mechanisms, transcriptional corepressors also associate with actively transcribed genes. Here, we show that HOS15 is the substrate receptor for an SCF/CUL1 E3 ubiquitin ligase complex (SCFHOS15) that negatively regulates plant immunity by destabilizing transcriptional activation complexes containing NPR1 and associated transcriptional activators. In unchallenged conditions, HOS15 continuously eliminates NPR1 to prevent inappropriate defense gene expression. Upon defense activation, HOS15 preferentially associates with phosphorylated NPR1 to stimulate rapid degradation of transcriptionally active NPR1 and thus limit the extent of defense gene expression. Our findings indicate that HOS15-mediated ubiquitination and elimination of NPR1 produce effects contrary to those of CUL3-containing ubiquitin ligase that coactivate defense gene expression. Thus, HOS15 plays a key role in the dynamic regulation of pre- and postactivation host defense.

scholarly journals Characterization of a Diffusible Signal Capable of Inducing Defense Gene Expression in Tobacco

1997 ◽  
Vol 113 (2) ◽  
pp. 621-629 ◽  
Author(s):  
J. Chappell ◽  
A. Levine ◽  
R. Tenhaken ◽  
M. Lusso ◽  
C. Lamb
Keyword(s):  
Gene Expression ◽  
Defense Gene Expression ◽  
Defense Gene
Sign in / Sign up

Export Citation Format

Share Document