The influence of different doses n-3 fatty acids (n-3 FA) with and without supplementation of vitamin E (vit E) on the levels of reactive oxygen species (ROS) in circulating human leukocytes

Penyakit jantung koroner (PJK) terutama disebabkan oleh aterosklerosis karena hiperkolesterolimia. Oksidasi low density lipoprotein (LDL) oleh reactive oxygen species (ROS) sebagai penyebab utama proses aterogenik dapat dicegah dengan kehadiran antioksidan seperti vitamin E. Buah dan sayuran banyak mengandung vitamin. Salah satu buah yang mengandung vitamin E adalah buah merah (Pandanus conoideus Lam.). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak buah merah terhadap kadar LDL darah pada tikus putih (Rattus norvegicus L.). Jenis penelitian ini adalah penelitian eksperimental laboratorium yang didesain mengikuti Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 4 kali ulangan dengan parameter penelitian adalah kadar LDL darah tikus putih. Data dianalisis menggunakan one way anova dilanjutkan dengan uji Duncan 95%. Berdasarkan hasil penelitian, minyak buah merah memiliki kemampuan untuk menurunkan kadar LDL darah tikus putih.

Download Full-text

EFFECTS OF FATTY ACIDS ON PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) BY SKELETAL MUSCLE CELLS

The FASEB Journal ◽

10.1096/fasebj.24.1_supplement.849.6 ◽

2010 ◽

Vol 24 (S1) ◽

Author(s):

Renato Tadeu Nachbar ◽

Augusto Ducati Luchessi ◽

Tavane David Cambiaghi ◽

Rafael Herling Lambertucci ◽

Sandro Massao Hirabara ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Skeletal Muscle ◽

Fatty Acids ◽

Reactive Oxygen ◽

Muscle Cells ◽

Skeletal Muscle Cells ◽

Oxygen Species

Download Full-text

Reactive Oxygen Species (ROS)-Responsive Nanoprobe For Bioimaging And Targeted Therapy of Osteoarthritis

10.21203/rs.3.rs-607946/v1 ◽

2021 ◽

Author(s):

Chong Shen ◽

Ming Gao ◽

Haimin Chen ◽

Yanting Zhan ◽

Qiumei Lan ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cartilage Damage ◽

Reactive Oxygen ◽

Disease Classification ◽

Oxygen Species ◽

Stimulus Responsive ◽

Targeting Peptide ◽

Physiological Markers ◽

Different Doses ◽

Selection Of

Abstract Stimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is one of the leading causes of OA, we selected ROS as markers and designed a cartilage-targeting and ROS-responsive theranostic nanoprobe that is highly specific for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKC, which was then encapsulated with Dexamethasone (DEX) to form TKC@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKC@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics.

Download Full-text