Reactive Oxygen Species (ROS)-Responsive Nanoprobe For Bioimaging And Targeted Therapy of Osteoarthritis

2021 ◽  
Author(s):  
Chong Shen ◽  
Ming Gao ◽  
Haimin Chen ◽  
Yanting Zhan ◽  
Qiumei Lan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cartilage Damage ◽  
Reactive Oxygen ◽  
Disease Classification ◽  
Oxygen Species ◽  
Stimulus Responsive ◽  
Targeting Peptide ◽  
Physiological Markers ◽  
Different Doses ◽  
Selection Of

Abstract Stimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is one of the leading causes of OA, we selected ROS as markers and designed a cartilage-targeting and ROS-responsive theranostic nanoprobe that is highly specific for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKC, which was then encapsulated with Dexamethasone (DEX) to form TKC@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKC@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics.

scholarly journals Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Chong Shen ◽  
Ming Gao ◽  
Haimin Chen ◽  
Yanting Zhan ◽  
Qiumei Lan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cartilage Damage ◽  
Reactive Oxygen ◽  
Disease Classification ◽  
Oxygen Species ◽  
Targeting Therapy ◽  
Targeting Peptide ◽  
Physiological Markers ◽  
Different Doses ◽  
Selection Of

AbstractStimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is associated with the progression in OA, we selected ROS as markers and designed a cartilage targeting and ROS-responsive theranostic nanoprobe that can be used for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKCP, which was then encapsulated with Dexamethasone (DEX) to form TKCP@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKCP@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics. Graphical Abstract

scholarly journals Selection of Functional Human Sperm with Higher DNA Integrity and Fewer Reactive Oxygen Species

2014 ◽  
Vol 3 (10) ◽  
pp. 1671-1679 ◽  
Author(s):  
Waseem Asghar ◽  
Vanessa Velasco ◽  
James L. Kingsley ◽  
Muhammad S. Shoukat ◽  
Hadi Shafiee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Dna Integrity ◽  
Oxygen Species ◽  
Selection Of

scholarly journals GENERATION OF REACTIVE OXYGEN SPECIES IN RAT EPIDIDYMAL SPERMATOZOA AFTER CYCLOPHOSPHAMIDE TREATMENT

2018 ◽  
Vol 11 (4) ◽  
pp. 437
Author(s):  
Kashmiri Zn ◽  
Sastry Ms
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Morphology ◽  
Reactive Oxygen ◽  
Wistar Rat ◽  
Sperm Function ◽  
Oxygen Species ◽  
Cyclophosphamide Treatment ◽  
Epididymal Spermatozoa ◽  
Vehicle Treated Control ◽  
Different Doses

 Objective: The aim of this study was to examine the effect of an anticancer drug cyclophosphamide (CPA) on generation of reactive oxygen species (ROS) in epididymal spermatozoa of Wistar rat, Rattus norvegicus. Methods: For this purpose, the rats were injected intraperitoneally with different doses of CPA (5 mg, 15 mg, and 20 mg/Kg BW for 2 weeks).Results: Treatment resulted into significantly increased level of ROS in CPA-treated groups when compared to the vehicle-treated control group.Conclusion: The results revealed that CPA has deleterious effect on the sperm morphology and physiology, which is dose and duration dependent and at certain doses cause the production of a number of reactive molecules and free radicals derived from molecular oxygen consequently resulting into adverse effect on the sperm function and hence on reproduction.

ROS responsive EPO nanoparticle ameliorates ionizing radiation-induced hematopoietic injury

2021 ◽  
Author(s):  
Huiyang Li ◽  
Xiaoyu Liang ◽  
Jianwei Duan ◽  
Youlu Chen ◽  
Xinxin Tian ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Ionizing Radiation ◽  
Active Site ◽  
Drug Carrier ◽  
Reactive Oxygen ◽  
Polymer Materials ◽  
Oxygen Species ◽  
Stimulus Responsive ◽  
Responsive Polymer ◽  
Radiation Induced

Stimulus-responsive polymer materials have attracted much attention as drug carrier because of the ability to deliver drug to the active site. Reactive oxygen species (ROS) plays crucial roles in cellular...

Mechanisms associated with mitochondrial-generated reactive oxygen species in cancerThis article is one of a selection of papers published in a Special Issue on Oxidative Stress in Health and Disease.

2010 ◽  
Vol 88 (3) ◽  
pp. 204-219 ◽  
Author(s):  
Meghan L. Verschoor ◽  
Leigh A. Wilson ◽  
Gurmit Singh
Keyword(s):  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Transport Chain ◽  
Mitochondrial Signaling ◽  
Genes Encoding ◽  
Mapk Phosphatases ◽  
Health And Disease ◽  
Selection Of

The mitochondria are unique cellular organelles that contain their own genome and, in conjunction with the nucleus, are able to transcribe and translate genes encoding components of the electron transport chain (ETC). To do so, the mitochondria must communicate with the nucleus via the production of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2), which are produced as a byproduct of aerobic respiration within the mitochondria. Mitochondrial signaling is proposed to be altered in cancer cells, where the mitochondria are frequently found to harbor mutations within their genome and display altered functional characteristics leading to increased glycolysis. As signaling molecules, ROS oxidize and inhibit MAPK phosphatases resulting in enhanced proliferation and survival, an effect particularly advantageous to cancer cells. In terms of transcriptional regulation, ROS affect the phosphorylation, activation, oxidation, and DNA binding of transcription factors such as AP-1, NF-κB, p53, and HIF-1α, leading to changes in target gene expression. Increased ROS production by defective cancer cell mitochondria also results in the upregulation of the transcription factor Ets-1, a factor that has been increasingly associated with aggressive cancers.

scholarly journals EFEKTIVITAS EKSTRAK PROPOLIS DALAM PENYEMBUHAN LUKA BAKAR DERAJAT II PADA HEWAN UJI

2021 ◽  
Vol 1 (2) ◽  
pp. 80
Author(s):  
Komang Sherly Ulandari ◽  
Putu Cahyani Paramita Yoga ◽  
Made Widya Lestari ◽  
Ni Nyoman Mestri Agustini
Keyword(s):  
Reactive Oxygen Species ◽  
Ferulic Acid ◽  
Tissue Repair ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Literature Study ◽  
Propolis Extract ◽  
Average Value ◽  
Different Doses ◽  
Second Degree

Luka bakar merupakan salah satu insiden yang sering terjadi dalam kehidupan sehari-hari. Tujuan dari penelitian berdasarkan studi pustaka ini adalah untuk mengetahui efektivitas ekstrak propolis dalam penyembuhan luka bakar derajat II pada hewan uji. Propolis kaya akan manfaat seperti menjadi antimikroba, antibakteri, antimikotik, astringent, spasmolitik, anti-inflamasi, anestesi, antioksidan, antiulcer, antikanker, serta memiliki efek imunomodulator yang dapat mendukung penyembuhan luka bakar derajat II. Senyawa flavonoid, fenol, arginine, asam ferulat, dan albumin berperan baik dalam menurunkan pertumbuhan mikroorganisme, mempercepat perbaikan jaringan dan menghambat munculnya ROS atau Reactive Oxygen Species pada luka bakar.Ekstrak propolis dengan konsentrasi 100% telah terbukti lebih efektif dalam mempercepat penyembuhan luka bakar derajat II pada hewan uji. Pemberian propolis dengan dosis pemberian yang berbeda menunjukkan perbedaan lamanya waktu penyembuhan luka bakar yang ditandai oleh perbedaan nilai rata-rata luas luka bakar dalam setiapwaktu pengukuran. Berdasarkan analisis hipotesis peneliti, efektivitas penyembuhan luka bakar dipengaruhi oleh dosis pemberian ekstrak propolis pada luka bakar tersebut. Dimana keduanya memiliki hubungan yang sebanding, peningkatan dosis pemberian propolis dapat mempercepat penyembuhan luka bakar derajat II.Burns are one of the incidents that often occur in life. The purpose of this research based on literature study was to determine the effectiveness of propolis extract in healing second degree burns in tested animals. Propolis is rich in benefits such as being antimicrobial, antibacterial, antimycotic, astringent, spasmolytic, anti- inflammatory, anesthetic, antioxidant, antiulcer, anticancer, and has an immunomodulatory effect that can support healing of second degree burns. Flavonoids, phenols, arginine, ferulic acid, and albumin have a good role in reducing the growth of microorganisms, accelerating tissue repair and inhibiting the appearance of ROS or Reactive Oxygen Species in burns.Propolis extract with a concentration of 100% has been proven to be more effective in accelerating the healing of second degree burns in test animals. Giving propolis with different doses showed a difference in length of time to heal burns, indicated by differences in the average value of burns in each measurement time. Based on the analysis of the researchers' hypothesis, the effectiveness of healing burns was influenced by the dose of propolis extract on the burns. Both have an equal relation, increasing thedose of giving propolis can accelerate the healing of second degree burns Keywords: second degree burns, propolis, effectiveness of propolisextrac

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species

Clinical aspects of reactive oxygen and nitrogen species

2004 ◽  
Vol 71 ◽  
pp. 121-133 ◽  
Author(s):  
Ascan Warnholtz ◽  
Maria Wendt ◽  
Michael August ◽  
Thomas Münzel
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Endothelial Dysfunction ◽  
Vascular Tissue ◽  
Rapid Development ◽  
Reactive Oxygen ◽  
Clinical Aspects ◽  
No Production ◽  
Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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