PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (1) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

A TECHNIQUE OF PREPARATIVE PAPER CHROMATOGRAPHY

1966 ◽  
Vol 44 (2) ◽  
pp. 149-153 ◽  
Author(s):  
A. E. Pasieka ◽  
J. E. Logan
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Biological Materials ◽  
High Salt ◽  
Salt Medium ◽  
Preparative Scale ◽  
High Concentrations ◽  
Total Amino Acids

The use of a solvent redevelopment technique enables the separation of amino acids from complex biological materials in the presence of high concentrations of salts. By conventional chromatography, 1.17 mg of total amino acids have been separated from the high salt medium M 150. The preparative technique as described here has separated amounts as great as 1.17 g and the patterns are essentially the same as for the analytical types. The separations are effected by four or more successive 15- to 20-hour solvent developments with drying between each solvent stage before the staining of chromatograms or isolation of particular bands. The results of these solvent developments on the preparative scale are illustrated with photographs of actual chromatograms. This technique requires thick filter paper sheets up to 4 ft in length for analytical, and particularly for preparative, chromatograms.

Buffered Filter Paper Chromatography of Amino Acids

1951 ◽  
Vol 23 (1) ◽  
pp. 168-174 ◽  
Author(s):  
E. F. McFarren
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper

"One-" and '"Two-dimensional" Partition Chromatographic Separations of Organic Acids on an Inert Sheet Support

1948 ◽  
Vol 1 (1) ◽  
pp. 98 ◽  
Author(s):  
JWH Lugg ◽  
BT Overell
Keyword(s):  
Organic Acids ◽  
Filter Paper ◽  
Volatile Acid ◽  
Two Dimensional ◽  
High Concentration ◽  
Chromatographic Separations ◽  
One Dimensional ◽  
Volatile Organic ◽  
Rf Values ◽  
Volatile Organic Acids

Procedures have been described for "one-" and "two-dimensional" partition-chromatographic separations, on an inert sheet (paper) support, of mixtures of relatively non-volatile organic acids. Ionization, and adsorption of the acids by the paper, have been suppressed by swamping both the stationary and mobile solvent phases with a volatile acid (acetic or formic). Formic acid is superior in that, at high concentration, it permits welldefined bands of the test acids to travel down the sheets and confers upon the bands highly characteristic RF values. RF values for some acids (malic, citric, tartaric, succinic, fumaric, malonic, pyruvic, lactic, ketosuccinic (oxalacetic), α-ketoglutaric, monomethyl succinic, glutaric, and adipic) at 20°C., when Whatman No. 1 filter paper and various pairs of solvent phases are used. have been provided. Some of the acids present in carrot tissue have been separated in "one-dimensional" work and identified by their RF values.

Two-Dimensional Thin-Layer Chromatography on Two-Layer Plates of Amino Acids

1970 ◽  
Vol 16 (8) ◽  
pp. 662-666 ◽  
Author(s):  
F Kraffczyk ◽  
R Helger ◽  
H Lang
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Two Dimensions ◽  
Two Dimensional ◽  
One Dimensional ◽  
Acid Cation ◽  
Solvent Systems ◽  
Strong Acids ◽  
Layer Chromatography

Abstract Separation of the amino acids in urine by use of thin-layer chromatography (TLC) has hitherto required that the specimen be first desalted and then chromatographed in two dimensions with at least two pairs of developing solvent systems. We wished to simplify both steps. The customary method of desalting on a column is replaced by desalting on a plate that supports a strongly acid cation-exchanger and a cellulose layer. This method, originally developed for one-dimensional TLC, is used here for two-dimensional TLC. Urine is applied to the ion-exchange layer and strong acids and neutral substances are removed with water. The amino acids are then chromatographed into the cellulose layer, and are separated there two dimensionally with a newly devised pair of developing solutions. This pair of solvents separates nearly all of the amino acids in urine.

ESSENTIAL AMINO ACIDS IN MICROORGANISMS

1957 ◽  
Vol 3 (5) ◽  
pp. 721-728 ◽  
Author(s):  
F. Reusser ◽  
J. F. T. Spencer ◽  
H. R. Sallans
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Method ◽  
Essential Amino Acids ◽  
Cellular Protein ◽  
One Dimensional ◽  
Low Concentrations ◽  
Amino Acid Determination ◽  
High Concentrations ◽  
Acid Determination

The cells of 19 species of bacteria, actinomyces, and yeasts were analyzed for protein and essential amino acids. A rapid quantitative method for amino acid determination using one-dimensional paper chromatography was developed. The cellular protein from all species contained relatively high concentrations of lysine, somewhat lower concentrations of tryptophan and threonine, and very low concentrations of methionine. All of the 10 essential amino acids were found in each species tested, although individual differences in the relative and absolute amounts were observed.

Destruction of Amino Acids during Filter Paper Chromatography

Science ◽  
1951 ◽  
Vol 113 (2923) ◽  
pp. 4-6 ◽  
Author(s):  
M. K. Brush ◽  
R. K. Boutwell ◽  
A. D. Barton ◽  
C. Heidelberger
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper
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