scholarly journals The Phenomenon of Medical Waste Recycling in Indonesia: Contact Time and Chlorine Dose as a Disinfectant with The Bio-Indicator Bacillus subtilis and Bacillus stearothermophilus

2021 ◽  
Vol 22 (4) ◽  
pp. 47-58
Author(s):  
Elanda Fikri ◽  
Iis Kurniati ◽  
Wartiniyati Wartiniyati ◽  
Teguh Prijanto ◽  
Pujiono Pujiono ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Contact Time ◽  
Bacillus Stearothermophilus ◽  
Waste Recycling ◽  
Medical Waste

Further Evidence for the Structure of the Teichoic Acids from Bacillus stearothermophilus B65 and Bacillus subtilis var. niger WM

1978 ◽  
Vol 85 (2) ◽  
pp. 433-436 ◽  
Author(s):  
Willem R. BOER ◽  
Jan T. M. WOUTERS ◽  
Alistair J. ANDERSON ◽  
A. Ronald ARCHIBALD
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Stearothermophilus ◽  
Teichoic Acids

scholarly journals In Vivo and In Vitro evaluation of the efficacy of a peracetic acid-based disinfectant for decontamination of acrylic resins

2006 ◽  
Vol 17 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Ana Lúcia Campani Chassot ◽  
Maria Inês Pereira Poisl ◽  
Susana Maria Werner Samuel
Keyword(s):  
Bacillus Subtilis ◽  
Peracetic Acid ◽  
Bacillus Stearothermophilus ◽  
Acrylic Resin ◽  
Human Saliva ◽  
Acrylic Resins ◽  
The Media ◽  
High Level

The purpose of this study was to assess the antimicrobial efficacy of a peracetic acid-based disinfectant for decontamination of heat-polymerized, chemically activated and microwave-polymerized acrylic resins. Resin plates were contaminated in vivo upon intraoral use by 10 volunteers for 7 nights and slabs were contaminated in vitro by contact with Bacillus subtilis and Bacillus stearothermophilus. The contaminated acrylic resin specimens were immersed in a 0.2% peracetic acid-based disinfectant (Sterilife®; Lifemed) for 5 min or 10 min and placed in a BHI culture medium. After incubation at 37°C for 48 h, bacterial growth was assessed by analyzing turbidity of the medium. For all types of acrylic resin, no turbidity of the medium was observed for any of the resin specimens immersed in the peracetic acid-based disinfectant for either 5 or 10 min. On the other hand, the media with specimens that were not immersed in the disinfectant (control) showed turbidity in 100% of the cases, indicating the presence of microorganisms in both tested conditions. In conclusion, immersion for at least 5 min in a 0.2% peracetic acid-based disinfectant promoted high-level disinfection of heat-polymerized, chemically activated and microwave-polymerized acrylic resins contaminated with either human saliva or Bacillus subtilis or Bacillus stearothermophilus.

Sustainable Solid Waste Management in Bangladesh

2020 ◽  
pp. 35-55
Author(s):  
Md. Ashikuzzaman ◽  
Md. Hasan Howlader
Keyword(s):  
Waste Management ◽  
Solid Waste ◽  
Management Practices ◽  
Solid Waste Management ◽  
Waste Recycling ◽  
Medical Waste ◽  
Community Based ◽  
Emerging Issues ◽  
Per Capita ◽  
Waste Management Practices

Solid waste management is a grave concern for Bangladesh as by 2025 waste generation per capita will be 0.75 kg/capita/day and total amount of waste will reach 21.07 million tons per year. This chapter attempts to uncover the facts regarding waste management along with the policies and regulations existing in Bangladesh by reviewing published secondary documents. The chapter also contains emerging issues of agricultural, industrial, hazardous, construction, and electronic generation and 3R practices in Bangladesh. Legal instruments for waste management in Bangladesh are also examined. It has been found that enforcing authorities lack the capacity to implement their strategies regarding 3R practices for waste management. Case studies about community-based approach, waste recycling sector, and medical waste management have been exemplified in this chapter. Finally, the authors apprised the issues and challenges of sustainable solid waste management practices and proposed the way forward for Bangladesh to have sustainable solid waste management.

Evaluation of Methods Used to Detect Antibiotic Residues in Milk1

1981 ◽  
Vol 44 (9) ◽  
pp. 696-698 ◽  
Author(s):  
D. M. MACAULAY ◽  
V. S. PACKARD
Keyword(s):  
Bacillus Subtilis ◽  
False Positive ◽  
Bacillus Stearothermophilus ◽  
Assay Method ◽  
Antibiotic Residues ◽  
Positive Results ◽  
Evaluation Of Methods ◽  
Disc Assay

Over nine replicate trials, five methods for detecting antibiotic residues were evaluated for sensitivity to various levels of penicillin, erythromycin and chloramphenicol in milk. The methods included (a) the Charm test, (b) Delvotest P, (c) disc assay with Bacillus subtilis on whey agar, (d) B. subtilis on Antibiotic Medium #1 and (e) Difco disc assay method using Bacillus stearothermophilus var. calidolactis on PMI agar. Specific to penicillin only, the Charm test detected the antibiotic 100% of the time down to and including 0.01 unit/ml, dropping to 56% detectability at 0.0025 unit/ml. Delvotest P detected penicillin 100% of the time at 0.025 unit/ml, but at varying degrees of sensitivity through 0.0025 unit/ml, and with evidence of showing false-positive results. Methods (c), (d) and (e) detected penicillin at 0.05 unit/ml 78, 89 and 100% of the time, respectively. Methods (d) and (e) were generally more sensitive to erythromycin and chloramphenicol than either (b) or (c).

Assessing the Disinfecting Power of Chlorite in Drinking Water

2006 ◽  
Vol 41 (4) ◽  
pp. 375-382 ◽  
Author(s):  
Françoise Bichai ◽  
Benoit Barbeau
Keyword(s):  
Drinking Water ◽  
Bacillus Subtilis ◽  
Contact Time ◽  
Bacteriostatic Effect ◽  
Bacterial Regrowth

Abstract The goal of this study was to review and confirm experimentally chlorite effectiveness as a drinking water disinfectant. The steps in the experiment were a series of laboratory assays performed on three types of microorganisms, Bacillus subtilis spores, MS2 coliphages, as well as heterotrophic (HPC) bacteria, to verify chlorite action in water. The tests showed that chlorites have no disinfectant effect on B. subtilis spores (CT > 106 mg min/L), that they slightly decrease HPC bacterial regrowth and, finally, that a dose of 1 and 10 mg ClO2-/L can inactivate almost 2 log and 4.5 log of MS2 phages, respectively, after 9 days of contact time. It would therefore appear that chlorites are not a good primary disinfectant, but do exhibit a bacteriostatic effect.

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