Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

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Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

Jurnal Kimia VALENSI ◽

10.15408/jkv.v1i1.19866 ◽

2021 ◽

Vol 1 (1) ◽

pp. 10-21

Author(s):

Ismiyarto Ismiyarto ◽

Niken Windi Saputri ◽

Liswinda Zafirah Rahmatia ◽

Purbowatiningrum Ria Sarjono ◽

Ngadiwiyana Ngadiwiyana ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Schiff Base ◽

Carboxymethyl Chitosan ◽

Diffusion Method ◽

Clear Zone ◽

Disc Diffusion Method ◽

Zone Diameter ◽

Chitosan Schiff Base

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ANTIBACTERIAL ACTIVITY Streptomyces spp. ENDHOPHYTIC TO Staphylococcus aureus AND Eschericia coli

JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER ◽

10.53864/jifakfar.v4i1.50 ◽

2021 ◽

Vol 4 (1) ◽

pp. 19-25

Author(s):

Siti Nur Azizah

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Diffusion Method ◽

Clear Zone ◽

Zone Diameter ◽

Streptomyces Spp ◽

Agar Plug ◽

Streptomyces Griseoruber

The objective of this research was to known antibacterial activity of 4 endhophytic Streptomyces namely Streptomyces griseorubiginosus, Streptomyces vellosus, Streptomyces diastaticus and Streptomyces griseoruber against Staphylococcus aureus and Eschericia coli and measure the biggest antibacterial activity which is indicate by clear zone diameter. Antibacterial activity by using agar plug diffusion method showed that Streptomyces vellosus has capable to inhibits growth of Staphylococcus aureus but not with Eschericia coli and Streptomyces diastaticus has capable to inhibits growth of Eschericia coli but not with Staphylococcus aureus. The clear zone of Streptomyces vellosus and Streptomyces diastaticus are 5,18 mm and 7 mm respectively. Streptomyces griseoruber and Streptomyces griseorubiginosus can’t grow during isolation. Keyword: Streptomyces vellosus, Streptomyces diastaticus, agar plug diffusion method

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Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

Prosiding Seminar Nasional Kesehatan ◽

10.48144/prosiding.v1i.711 ◽

2021 ◽

Vol 1 ◽

pp. 547-553

Author(s):

Hida Ilyana ◽

Dwi Bagus Pambudi

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Extraction Method ◽

Research Method ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Clear Zone ◽

Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

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AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli

Jurnal Sains Natural ◽

10.31938/jsn.v6i1.253 ◽

2019 ◽

Vol 6 (1) ◽

pp. 33

Author(s):

Yuni Widyastuti ◽

Nia Yuliani ◽

I.G.A. Manik Widhyastini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Aloe Vera ◽

Bacterial Count ◽

Inhibition Zone ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Escherichia Coli Bacteria

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan Escherichia coli, Infusa.

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What about the Antibacterial Activity of Fresh Ginger Juice (Zingiber officinale)?

Journal of Advances in Medicine and Medical Research ◽

10.9734/jammr/2021/v33i1931092 ◽

2021 ◽

pp. 163-172

Author(s):

E. Günaydın ◽

Gülşen Goncagül ◽

Çiğdem Güceyü ◽

Ö. Kardoğan

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Intensive Care ◽

Antibacterial Effect ◽

Zingiber Officinale ◽

Diffusion Method ◽

Disc Diffusion ◽

Antibacterial Effects ◽

Disc Diffusion Method

Background: The aim of this study was to determine the antibacterial activity of the juice obtained by squeezing fresh ginger roots (Zingiber officinale) against the standard strains Staphylococcus aureus (ATCC® 25923) and Escherichia coli (ATCC® 25922), as well as Acinetobacter baumannii and Klebsiella pneumoniae isolated from patients hospitalized in intensive care units. Methods: For this purpose, the antibacterial effect of the fresh ginger juice was tested against antibiotics such as ampicillin, cefazolin, cefuroxime, meropenem, colistin, ofloxacin, sulfamethoxazole/trimethoprim, tetracycline, and gentamicin. The antibacterial effects against the tested bacteria were determined by the disc diffusion method using 5 µL, 10 µL, 15 µL and 20 µL amounts of ginger juice impregnated on sterile discs. Results: As a result of the study, it was detected that the antibacterial effect increased in parallel with the increasing amounts of fresh ginger juice in all tested bacteria. Conclusion: Consequently, fresh Zingiber officinale can provide protection to a certain extent against bacterial pathogens due to its antibacterial action.

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Aktivitas Antibakteri dan Antioksidan Asam Galat dari Kulit Buah Lokal yang Diproduksi dengan Tanase

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.1.11300.148-159 ◽

2018 ◽

Vol 14 (1) ◽

pp. 148

Author(s):

Eka Junaidi ◽

Yunita Arian Sani Anwar

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Gallic Acid ◽

Diffusion Method ◽

Syzygium Cumini ◽

Garcinia Mangostana ◽

Dpph Method ◽

Fruit Waste

Penelitian ini merupakan lanjutan dari penelitian sebelumnya tentang produksi asam galat dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (Baccaurea racemosa Muell.Arg), kulit buah juwet (Syzygium cumini), dan kulit buah manggis (Garcinia mangostana). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC50 masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri Escherichia coli dan Staphylococcus aureus yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.Antibacterial and antioxidant activity of gallic acid from peel local fruit which produced by tannase. This research is a continuation of the previous research about the gallic acid production from peel fruit local in Lombok. The local fruit waste used are kepundung rind (Baccaurea racemosa Muell.Arg), juwet rind (Syzygium cumini) and mangosteen rind (Garcinia mangostana). In this research, the produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method to bacteria Escherichia coli and Staphylococcus aureus. The results show that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC50 values of 5,95; 5,96; and 5,92, respectively. Gallic acid not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result show that gallic acid from local fruit waste did not provide an antibacterial activity.

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EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

10.36106/paripex/2805137 ◽

2021 ◽

pp. 77-79

Author(s):

Janardhan Namdeo Nehul

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Methanol Extract ◽

Axenic Culture ◽

Soil Samples ◽

Diffusion Method ◽

Disc Diffusion ◽

Zone Of Inhibition ◽

Disc Diffusion Method ◽

Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

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AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽

10.35799/pha.8.2019.29326 ◽

2019 ◽

Vol 8 (3) ◽

pp. 525

Author(s):

Ayu Natasya Paputungan ◽

Widya Astuty Lolo ◽

Imam Jayanto

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Fraction ◽

Diffusion Method ◽

Garcinia Mangostana ◽

Methanol Fraction ◽

Tlc Bioautography ◽

Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography. ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

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Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus

Journal of Medical Microbiology ◽

10.1099/jmm.0.46965-0 ◽

2007 ◽

Vol 56 (7) ◽

pp. 937-939 ◽

Cited By ~ 24

Author(s):

Naira Elane Moreira de Oliveira ◽

Ana Paula Couto Marques Cardozo ◽

Elizabeth de Andrade Marques ◽

Kátia Regina Netto dos Santos ◽

Marcia Giambiagi deMarval

Keyword(s):

Staphylococcus Aureus ◽

High Resistance ◽

The Other ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Zone Diameter ◽

Resistant Strains ◽

Mupirocin Resistance ◽

High Level

Meticillin-resistant Staphylococcus aureus isolates were classified into three mupirocin susceptibility groups by the disc diffusion method using 5 and 200 μg mupirocin discs. The zone diameter observed for a 5 μg disc distinguished MupS from the resistant strains (either MupRL or MupRH). On the other hand, a 200 μg disc distinguished the high-resistance MupRH strains from the other two (MupS or MupRL). Thus, the concomitant use of 5 and 200 μg mupirocin discs allowed the clear distinction among the three mupirocin susceptibility groups, MupS, MupRL or MupRH.

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Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

Materials Science Forum ◽

10.4028/www.scientific.net/msf.901.124 ◽

2017 ◽

Vol 901 ◽

pp. 124-132

Author(s):

Artania Adnin Tri Suma ◽

Tutik Dwi Wahyuningsih ◽

Deni Pranowo

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Bacillus Cereus ◽

Shigella Flexneri ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Diffusion Method ◽

Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

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