scholarly journals ANTIBACTERIAL ACTIVITY Streptomyces spp. ENDHOPHYTIC TO Staphylococcus aureus AND Eschericia coli

2021 ◽  
Vol 4 (1) ◽  
pp. 19-25
Author(s):  
Siti Nur Azizah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Clear Zone ◽  
Zone Diameter ◽  
Streptomyces Spp ◽  
Agar Plug ◽  
Streptomyces Griseoruber

The objective of this research was to known antibacterial activity of 4 endhophytic Streptomyces namely Streptomyces griseorubiginosus,   Streptomyces vellosus, Streptomyces diastaticus and Streptomyces griseoruber against Staphylococcus aureus and Eschericia coli and measure the biggest antibacterial activity which is indicate by clear zone diameter. Antibacterial activity by using agar plug diffusion method showed that Streptomyces vellosus has capable to inhibits growth of Staphylococcus aureus but not with Eschericia coli and Streptomyces diastaticus has capable to inhibits growth of Eschericia coli but not with Staphylococcus aureus. The clear zone of  Streptomyces vellosus and          Streptomyces diastaticus are 5,18 mm and 7 mm respectively.                Streptomyces griseoruber and Streptomyces griseorubiginosus can’t grow during isolation.  Keyword: Streptomyces vellosus, Streptomyces diastaticus, agar plug diffusion method

scholarly journals Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

2021 ◽  
Vol 7 (1) ◽  
pp. 10-21
Author(s):  
Ismiyarto Ismiyarto ◽  
Niken Windi Saputri ◽  
Liswinda Zafirah Rahmatia ◽  
Purbowatiningrum Ria Sarjono ◽  
Ngadiwiyana Ngadiwiyana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Carboxymethyl Chitosan ◽  
Diffusion Method ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Chitosan Schiff Base

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain  Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

scholarly journals Synthesis of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base Salicylaldehyde and Antibacterial Activity

2021 ◽  
Vol 1 (1) ◽  
pp. 10-21
Author(s):  
Ismiyarto Ismiyarto ◽  
Niken Windi Saputri ◽  
Liswinda Zafirah Rahmatia ◽  
Purbowatiningrum Ria Sarjono ◽  
Ngadiwiyana Ngadiwiyana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Carboxymethyl Chitosan ◽  
Diffusion Method ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Zone Diameter ◽  
Chitosan Schiff Base

The development of compounds with a better antibacterial activity is highly needed. One way to achieve this is by modifying the structure of the compound using chitosan as a starting material, because of its abundant natural source in Indonesia, its biodegradable properties, and its structure where free amines are present. This study aims to obtain  Mn(II) -Carboxymethyl Chitosan Schiff Base-Salicylaldehyde complex to increase its antibacterial activity against Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative). Schiff Base carboxymethyl chitosan-salicylaldehyde was synthesized from carboxymethyl chitosan with salicylaldehyde. Next, the Schiff Base Carboxymethyl Chitosan-Salicylaldehyde was complexed with MnCl2.4H2O and then characterized by FTIR, UV-Vis Spectrophotometer, and AAS and tested for antibacterial activity with the disc diffusion method against Staphylococcus aureus and Escherichia coli. The product of Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a brownish yellow solid with a yield of 64% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 11 mm) and Escherichia coli (clear zone diameter 13 mm). The product of Mn(II) Complexes-Carboxymethyl Chitosan Schiff Base-salicylaldehyde is a black solid with a yield of 59% (w/w) and has antibacterial activity against Staphylococcus aureus (clear zone diameter 13 mm) and Escherichia coli (clear zone diameter 17 mm).

scholarly journals PENGARUH PEMBENTUK FILM PVA PADA SEDIAAN MASKER GEL PEEL-OFF EKSTRAK ETANOL BAWANG DAYAK (Eleutherina palmifolia (L.) Merr) TERHADAP AKTIVITAS ANTIBAKTERI Staphylococcus aureus PENYEBAB JERAWAT

2018 ◽  
Vol 15 (2) ◽  
pp. 24
Author(s):  
Husnani Husnani ◽  
Fitri Sri Rizki
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Polyvinyl Alcohol ◽  
Ethanol Extract ◽  
Clear Zone ◽  
Onion Extract ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Inhibitory Power

ABSTRACTDayak Onion (Eleutherine palmifolia) is one of the plants that can inhibit Staphylococcus aureus bacteria that causes acne. This study aims to determine the effect of polyvinyl alcohol on peel-off gel mask preparations from onion dayak ethanol extract on the antibacterial activity of Staphylococcus aureus that causes acne. Extraction using maceration method with 96% ethanol. The concentration of dayak onion extract was made at 5%, 7.5%, and 10% and the peel-off gel mask formula from dayak onion extract with variations in extract concentration were 5%, 7.5%, and 10% based on Polyvinyl Alcohol (PVA). Antibacterial activity is carried out using the disk disc method. In testing the inhibition of dayak onion extract at a concentration of 5% resulted in a clear zone of 15.5 mm, a concentration of 7.5% and 10% producing the same clear zone diameter of 17.1 mm. The peel-off gel mask in formulation I obtained a clear zone diameter of 8.7 mm, formulation II the diameter of the clear zone was 12.2 mm, and formula III the diameter of the clear zone was 15.3 mm. The results of this study indicate that on dayak onion extract has a greater inhibitory power compared to the dayak onion extract peel-off gel mask preparation. It can be concluded that the PVA in the preparation affects the amount of inhibitory power on the bacteria forming acne, namely Staphylococcus aureus.Keywords: Dayak onion extract, Staphylococcus aureus, disk discs, antibacterial inhibitory test, acne-causing bacteria, peel-off gel mask.

scholarly journals Antibacterial Activity of Honey on Staphylococcus aureus and Streptococcus pyogenes Isolated from Wound Pus

2020 ◽  
Vol 2 (1) ◽  
pp. 38
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Honey Sample ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Test Organisms ◽  
Treatment Of Wounds ◽  
Agar Well Diffusion Method

Antibacterial activity of honey obtained from an apiculture farm was tested on Staphylococcus aureus and Streptococcus pyogens isolated from wound pus was observed. Agar well diffusion method was used to determine the antibacterial activity of honey on the test organisms. Moreover, the result was that the honey sample had heavy antibacterial activities against the test organisms, which was revealed by the zone of inhibition obtained. The antibacterial activity increased with the increase in concentrations of honey and showed a clear zone for both organisms. Therefore honey can be accepted as an antibacterial agent for the treatment of wounds, burns, bedsores, and ulcers.

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals POTENSI BATANG TANAMAN YODIUM (Jatropha multifida Linn) SEBAGAI SENYAWA ANTIBAKTERI Staphylococcus aureus ATCC 25923 SECARA IN VITRO

2019 ◽  
Vol 3 (1) ◽  
pp. 1-6
Author(s):  
Rokhana Rokhana ◽  
Ainiyah Ainiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Experimental Research ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Clear Zone ◽  
Plant Stem ◽  
Jatropha Multifida

The search for bioactive compounds sources it continue to do because of the emergence of pathogenic bacteria that are resistant to antibiotics. Iodine plants(Jatropha multifida Linn) has flavonoids, alkaloids, tannins, saponins and jatropins. The flavanoid content in iodine plants can inhibit bacterial growth. Staphylococcus aureus is a pathogenic bacterium that can cause infections characterized by tissue damage accompanied by purulent abscesses. The purpose of this study was to determine the antibacterial activity of methanol extract of iodine plant stem against Staphylococcus aureus in vitro. Types of laboratory experimental research. The sample used in the study was iodine plant (Jatropha multifida Linn) extrac concentration used 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. Using the Kirby bauer diffusion method. The results showed that there was antibacterial activity on iodine stems which showed a clear zone around the cactam disk at concentrations of 40% (10mm) 60% (12 mm), 80% (14mm), 100% (17 mm).

scholarly journals Aktivitas Antibakteri Kain Poliester dengan Penambahan Heksadesiltrimetoksisilan (HDTMS) terhadap Staphylococcus aureus ATCC 25924

2017 ◽  
pp. 95-100
Author(s):  
Eli Rohaeti ◽  
Nur Ihda Zulaikha
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Incubation Time ◽  
Polyester Fabric ◽  
Diffusion Method ◽  
Test Results ◽  
Clear Zone ◽  
Polyester Fabrics ◽  
Activity Test ◽  
Fabric Sample

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kain poliester yang terdeposit senyawa HDTMS (P-HDTMS) terhadap bakteri Staphylococcus aureus ATCC 25924. Larutan HDTMS dibuat dengan melarutkan senyawa HDTMS dalam etanol 4%. Larutan HDTMS kemudian didepositkan pada sampel kain Poliester. Uji aktivitas antibakteri P-HDTMS dilakukan melalui metode difusi dengan mengukur diameter zona bening di sekitar sampel. Hasil penelitian menunjukkan bahwa senyawa HDTMS telah terdeposit pada kain poliester. Hasil uji zona bening menunjukkan bahwa P-HDTMS memiliki diameter zona bening sebesar 9.25 mm pada waktu inkubasi selama 60 jam. Dengan demikian P-HDTMS mempunyai aktivitas antibakteri dalam menghambat pertumbuhan bakteri Staphylococcus aureus ATCC 25924.This research aims to determine the antibacterial activity of polyesterwhich was deposited by hexadecyltrimethoxysilane (HDTMS) against bacteria Staphylococcus aureus ATCC 25924.The HDTMS solution was made by dissolving the HDTMS compound in 4% ethanol. The HDTMS solution was then deposited on a polyester fabric sample. The antibacterial activity test of P-HDTMS was conducted with diffusion method by measuring the diameter of clear zone around the sample.The results of this researchshowed that HDTMS compound has been deposited on polyester fabrics. The clear zone test results showed that P-HDTMS has diameter of clear zone of 9.25 mm at incubation time of 60 hours. Thus, P-HDTMS has an antibacterial activity against Staphylococcus aureusATCC 25924.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals In VitroAntibacterial Potential of Extracts ofSterculia africana,Acacia sieberiana, andCassia abbreviatassp.abbreviataUsed by Yellow Baboons(Papio cynocephalus)for Possible Self-Medication in Mikumi National Park, Tanzania

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Irene Kirabo ◽  
Faith P. Mabiki ◽  
Robinson H. Mdegela ◽  
Christopher J. D. Obbo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Plant Extracts ◽  
National Park ◽  
Nonhuman Primates ◽  
Diffusion Method ◽  
Root Bark ◽  
Self Medication ◽  
Gram Positive ◽  
Acacia Sieberiana

In the animals in general and nonhuman primates in particular self-medication has been widely reported; however, little is still known about the pharmacological activity of the extracts present in their daily diet. Thein vitroantibacterial activity of the stem, root bark, and leaf extracts of three selected plants on which yellow baboons feed in an unusual manner in Mikumi National Park, Tanzania, was evaluated. Crude plant extracts were tested against Gram positive and Gram negative bacteria of medical and veterinary importance employing a modified agar well diffusion method and Minimum Inhibitory Concentration (MIC) technique. The lowest MIC value for Gram positive strains was 0.31 mg/ml demonstrated byCassia abbreviatassp.abbreviataagainstStaphylococcus aureus(ATCC 25923). The highest susceptibility to the ethanol plant extracts was exhibited byPseudomonas aeruginosa,Escherichia coli,andStaphylococcus aureus,examples of microbes that affect both human and nonhuman primates. These findings demonstrate that the plant extracts fromSterculia africana,Acacia sieberiana,andCassia abbreviatassp.abbreviatahave antibacterial activity and may be used as feed for their prophylactic benefits. Remarkably, the lowest MIC of 0.16 mg/ml was only 16-fold weaker than Gentamicin, a standard drug.

Sign in / Sign up

Export Citation Format

Share Document