Sensitivity of Seed Germination to Salt Stress in Teff [Eragrostis tef (Zucc.) Trotter]

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.

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Sensitivity of Seed Germination to Salt Stress in Black Cumin (Nigella sativa L.)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha46110861 ◽

2018 ◽

Vol 46 (1) ◽

pp. 202-205 ◽

Cited By ~ 4

Author(s):

Panayiota PAPASTYLIANOU ◽

Nikolia-Nonika BAKOGIANNI ◽

Ilias TRAVLOS ◽

Ioannis ROUSSIS

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Nigella Sativa ◽

Germination Percentage ◽

Oilseed Crop ◽

Black Cumin ◽

Germination Time ◽

Randomized Design ◽

Mean Germination Time ◽

Major Factors

Black cumin is an annual oilseed crop, native to the Mediterranean region and widely used for nutritional and medicinal purposes. Reduced seed germination and early seedling growth under saline conditions are considered as major factors limiting the establishment of crops. The objective of this study was to investigate the effect of salinity due to NaCl on germination of black cumin seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 20 °C) in the dark for 16 days. In order to create salt stress conditions during the germination process, five solutions were used (0 as control, 80, 160, 240 and 320 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The effect of salinity on seed germination percentage and mean germination time was significant at p<0.01. Germination of the controls was 96% and 95% at 15 °C and 20 °C, respectively. The presence of NaCl, regardless of temperature, reduced germination. This reduction was more severe at 15 °C (15%, and 19% germination in cases of 80 and 160 mM concentration respectively) than at 20 °C (65% at 80 mM salinity level) and no germination was observed from 240 mM onwards. The mean germination time increased with increased NaCl levels, especially at the lower temperature of 15 °C. The overall results of this experiment showed the inhibitory effects of salt stress on seed germination parameters of black cumin.

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Sensitivity of Seed Germination to Temperature and Light in Helichrysum amorginum

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2020.0007 ◽

2020 ◽

Vol 77 (1) ◽

pp. 7

Author(s):

Sofia ARGYRIOU ◽

Panayiota PAPASTYLIANOU ◽

George STAVROPOULOS ◽

Angeliki KOUSTA ◽

Ioanna KAKABOUKI ◽

...

Keyword(s):

Seed Germination ◽

Seedling Survival ◽

Germination Percentage ◽

Effect Of Temperature ◽

Germination Time ◽

Randomized Design ◽

Perennial Shrub ◽

Mean Germination Time ◽

Lower Temperature ◽

Seed Propagation

Helichrysum amorginum is a perennial shrub, endemic on the Greek island of Amorgos and used for medicinal, cosmetic and ornamental purposes. Information concerning germination behavior and seedling survival is considered essential for the establishment and cultivation of wild species. The objective of this study was to investigate the effect of temperature and light on germination of Helichrysum amorginum seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at three different suboptimal germination temperatures (15, 20 and 25°C) in light and dark conditions for 10 days. Seed material was collected from two different fields of the island (Aegiali and Katapola). The germination performance was evaluated by final germination percentage and mean germination time. The effect of temperature on seed germination percentage and mean germination time was significant in all cases. The presence of light enhanced germination, especially at the optimum temperature of 20°C. In most cases the mean germination time increased at the lower temperature of 15°C. Seeds from Aegiali exhibited higher germination ability than the seed lot from Katapola. Knowledge of seed germination behaviour is of major importance in order to establish effective protocols for seed propagation in Helichrysum amorginum.

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Morphometry and Germination of Passovia pyrifolia, Struthanthus marginatus and Phoradendron mucronatum Diaspores

Planta Daninha ◽

10.1590/s0100-83582018360100010 ◽

2018 ◽

Vol 36 (0) ◽

Author(s):

J.M.L. AZEVEDO ◽

C.G.G. SANTOS ◽

C.S. CAIRES ◽

J.C. ARAÚJO NETO ◽

R.C. SOUZA

Keyword(s):

Moisture Content ◽

Germination Rate ◽

Germination Percentage ◽

Percentage Germination ◽

Essential Characteristic ◽

Germination Time ◽

Randomized Design ◽

Different Temperatures ◽

Mean Germination Time ◽

Completely Randomized Design

ABSTRACT: The goal of this work was to characterize morphometrically diaspores from three species of mistletoes (Passovia pyrifolia, Struthanthus marginatus e Phoradendron mucronatum), as well as to study its germination behavior according to temperature and luminosity. In germination tests, the completely randomized design was used, in factor scheme 4 x 2, with 4 replications of 15 diaspores. The factors for the first test were different temperatures and two diaspore conditions (intact and without epicarp). In the second test, factors were different light qualities and two diaspore conditions. The analyzed variables were: germination percentage, germination rate index (GRI) and mean germination time (MGT). Mistletoe’s diaspores present an elliptic shape with viscin layer, an essential characteristic for the dispersion of the species. In addition to this, they present a distinction as for the moisture content when compared to the Loranthaceae and Viscaceae, where the latter presents the highest content. Germination is greater at the alternated temperature of 20-30 oC for P. pyrifolia and P. mucronatum diaspores and 20 oC for S. marginatus. Luminosity interfered in the germination of P. mucronatum and S. marginatus but did not influence P. pyrifolia. The diaspore condition influenced the germination of the three studied species, observing a significant increase in their germination when the epicarp was removed. Temperature and luminosity interfere directly in the GRI and MGT of the three mistletoes species.

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Salinity tolerance at germination of some main cultivated cowpea (Vigna unguiculata) genotypes from Western Cameroon

Annals of Plant Sciences ◽

10.21746/aps.2017.06.002 ◽

2017 ◽

Vol 6 (06) ◽

pp. 1634

Author(s):

Ebeny Leonny Tsague ◽

Eric Bertrand Kouam* ◽

Christopher Mubeteneh Tankou

Keyword(s):

Salt Stress ◽

Vigna Unguiculata ◽

Germination Percentage ◽

Randomized Design ◽

Nacl Concentration ◽

Mean Germination Time ◽

The Mean ◽

Coefficient Of Velocity ◽

Positive Correlations ◽

Osmotic Potentials

This study investigates the effect of salt (NaCl) on the germination of seeds of 10 genotypes of cowpea (Vigna unguiculata). The experiment carried out on petri dishes was done using a completely randomized design comprising three replications. The germination of the cultivars was studied using distilled water as control and under osmotic potentials of 50, 100, 150 and 200mM NaCl. The results indicated that the Mean Germination Time (MGT), Time to 50% Germination (T50), Germination Index (GI), Coefficient of Velocity of Germination (CVG), and Germination Percentage (GP), varied between 2.33 and 5.51 days; 2.83 and 14.87 days; 16.66 and 1.63; 0.53 and 0.11; 87.11 and 27.98% respectively. The variables GI, CVG and GP tended to decrease with increasing NaCl concentration while MGT and T50 tended to increase with increasing salt concentrations. For all the germination variables, significant differences at 0.001 probability levels were found among salinity treatments, cowpea genotypes and their interaction. Four genotypes (KEB-CP118, OU59A, OU100 and ICV12) were tolerant to salt stress and performed well under salinity conditions. Four other genotypes were found to be moderately tolerant (ET11, NO1036, MTA22 and KEB-CP009) and two genotypes (KEB-CP098 and NO193) were susceptible to salt stress and were most affected by salinity. At 0.001 probability level, positive correlations were observed between GP and GI (r = 0.792); GP and CVG (r = 0.774); GI and CVG (r = 0.955) and negative correlations were observed between GP and T50 (r = - 0.845); GI and MGT (r = - 0.894); CVG and MGT (r = - 0.895).

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Germination of Primed Seed under NaCl Stress in Wheat

ISRN Botany ◽

10.5402/2012/167804 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Michael P. Fuller ◽

Jalal H. Hamza ◽

Hail Z. Rihan ◽

Mohammad Al-Issawi

Keyword(s):

Salt Stress ◽

Germination Rate ◽

Arable Land ◽

Nacl Stress ◽

Germination Percentage ◽

Randomized Design ◽

Nacl Concentration ◽

Mean Germination Time ◽

Coefficient Of Velocity ◽

Germination Indices

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.

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Effects of Hydro and Hormonal Seed Priming on Seed Germination of Milk Thistle under Saline Stress Condition

Notulae Scientia Biologicae ◽

10.15835/nsb639372 ◽

2014 ◽

Vol 6 (3) ◽

pp. 374-380

Author(s):

Yousef NASIRI ◽

Parisa FEYZI ◽

Abdollah JAVANMARD

Keyword(s):

Salicylic Acid ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

Milk Thistle ◽

Saline Stress ◽

Nacl Salinity ◽

Germination Time ◽

Randomized Design ◽

Mean Germination Time

Salinity is an abiotic stress which has harmful effects on germination of many plants. Therefore, high germination rate and vigorous early growth under salty soils is preferred. Seed priming is a way to increase salt tolerance of plants. An experiment was conducted to investigate the effect of seed priming on germination of milk thistle under salinity condition. The treatments were 4 levels of seed priming (no priming, distilled water as hydro priming and 0.5 and 1.0 mM salicylic acid) and 5 levels of salinity (0, 40 and 80 mM NaCl and 40 and 80 mM CaCl2). The experiment arranged as a factorial in a completely randomized design (CRD) with three replications. Results showed that salinity decreased germination percentage and germination rate to about 16 and 32% in 80 mM CaCl2 level compared to control, respectively. The highest mean germination time (5.7 day) were belonged to 80 mM CaCl2. Radicle and plumule length significantly decreased by 80 mM NaCl and 40 and 80 mM CaCl2. The lowest seedling weight and seed stamina observed in 80 mM CaCl2. 0.5 mM salicylic acid improved all traits except mean germination time as compared to control. Salicylic acid (0.5 mM) improved radicle length under 0, 40 and 80 mM NaCl salinity levels as well as increased plumule length at the 0 and 40 mM NaCl salinity conditions.

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Seed Quality of Sorghum [Sorghum Bicolor (L.) Moesch] as Affected By Seed Priming and Storage

NASS Journal of Agricultural Sciences ◽

10.36956/njas.v3i1.310 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Awale Degewione Shirdon ◽

Khalid Ibrahim Omer ◽

R.P.S Tommer ◽

Ketema Belete

Keyword(s):

Seed Quality ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Quality Parameters ◽

Storage Duration ◽

Germination Time ◽

Randomized Design ◽

Mean Germination Time ◽

Almost All

Sorghum is being produced primarily as a food grain throughout the world. It is one of the major traditional food crops in Ethiopia. However, the productivity of the crop is very low and poor seed quality is one of the major determinants. An experiment study was conducted in laboratory and glasshouse to evaluate the effect of seed priming on seed quality parameters of three sorghum varieties:(Muyra-I, Muyra-II and Fendisha) with different storage of one year and two years using different priming agents with different soaking time for each priming agent (1.0mM KNO3, 1mM NaCl and distilled water for 72, 48 and 12 hours respectively) in 2012 at Haramaya University Seed laboratory. Completely randomized design in 2 x 3 x 4 factorial arrangement with three replications was used to carry out the germination and vigor tests. The priming concentrations and durations were based on previous work, which was effective in hydro and osmo-conditioning of sorghum seed. In the experiment, seed priming increased germination percentage (GP), Mean Germination Time (MGT) and improved seedling shoot (SSL) and root length (SRL), seedling dry weight (SDW) and seedling vigor indices in all varieties of both storage durations compared to their respective control. Priming with NaCl and KNO3 showed positive response followed by hydropriming for all varieties of different storage duration. When treated with sodium chloride, almost all the varieties of each storage year demonstrated an increase in germination and the degree of increment was higher for Muyra-I(__) and Fendisha (___), the lowest being for Muyra-II which was significantly lower for both storage year. In case of mean germination time Fendisha exhibited better with hydropriming for older seeds (___) and with KNO3 for newer seeds(___) while Muyra-II and Muyra-I showed better with KNO3 (____) for older seeds and with hydropriming for newer seeds. Responses of varieties for the remaining parameters were intermediate. Therefore, sorghum seed priming with osmo-media and water can compensate storage deterioration of sorghum seeds in addition to significantly improving the seed germination and vigor quality parameters

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EFFECT OF STORAGE TEMPERATURE AND CULTIVARS ON SEED GERMINATION OF Lilium×formolongi HORT.

Journal of Experimental Biology and Agricultural Sciences ◽

10.18006/2020.8(5).621.627 ◽

2020 ◽

Vol 8 (5) ◽

pp. 621-627

Author(s):

Rameshwar Rai ◽

◽

Jong Hwa Kim ◽

◽

...

Keyword(s):

Storage Temperature ◽

Great Influence ◽

Seed Storage ◽

Germination Percentage ◽

Temperature Treatment ◽

Germination Index ◽

Germination Time ◽

Long Term Storage ◽

Randomized Design ◽

Mean Germination Time

An experiment was carried out to evaluate the influences of seed storage temperature and variation due to cultivars on the germination of the Lilium×formolongi hort. seeds. The seeds of four cultivars of Lilium×formolongi Hort. viz. Augusta, Raizan Herald (RH), Eorayeon 1ho, and Eorayeon 2ho was treated with four temperature ranges 5°C, -2°C, -20°C and 20°C (Room temperature, Control) up to 6 months to execute the experiment. In total 500 seeds of each cultivar including four temperature regimes and four replications for each temperature treatment and each replication represented by 30 seeds have been provided 10 days chilling treatment before to test the germination to get the uniformity in germination. The laboratory experiment was carried out in a completely randomized design (CRD) to assess the different parameters of germination viz. germination percentage(GP), mean germination time (MGT), germination index (GI), time taken to 50% germination (T50), and coefficient of the velocity of germination (CVG) at four temperature regime of four cultivars. The ANOVA analysis revealed significant variability among the four cultivars for all germination parameters in all temperature treatment levels. The results of the experiment demonstrated that the storage temperature has a great influence on germination percentage (GP), mean germination time (MGT), germination index (GI), time taken to 50% germination (T50), and coefficient of the velocity of germination (CVG) with comparisons to the normal temperature stored seeds (20°C). The significant variation among these parameters for each cultivar demonstrated the significance of varied requirements like stored temperature for the long term storage of that particular cultivar.

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Phytotoxicity of Chitosan and SiO2 Nanoparticles to Seed Germination of Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) Plants

Notulae Scientia Biologicae ◽

10.15835/nsb9210075 ◽

2017 ◽

Vol 9 (2) ◽

pp. 242-249 ◽

Cited By ~ 5

Author(s):

Faride BEHBOUDI ◽

Zeinalabedin TAHMASEBI SARVESTANI ◽

Mohamad Zaman KASSAEE ◽

Seyed Ali Mohamad MODARES SANAVI ◽

Ali SOROOSHZADEH

Keyword(s):

Germination Rate ◽

Dry Weight ◽

Sio2 Nanoparticles ◽

Triticum Aestivum L ◽

Germination Percentage ◽

Hordeum Vulgare L ◽

Randomized Design ◽

Direct Exposure ◽

Contrast Exposure ◽

Mean Germination Time

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.

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Can Bulk and Nanosized Titanium Dioxide Particles Improve Seed Germination Features of Wheatgrass (Agropyron desertorum)

Notulae Scientia Biologicae ◽

10.15835/nsb539072 ◽

2013 ◽

Vol 5 (3) ◽

pp. 325-331 ◽

Cited By ~ 13

Author(s):

Reyhaneh AZIMI ◽

Hassan FEIZI ◽

Mohammad KHAJEH HOSSEINI

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Germination Time ◽

Positive Effects ◽

Tio2 Particles ◽

Nanosized Titanium Dioxide ◽

Mean Germination Time ◽

Germination Value ◽

Improve Seed Germination ◽

Nanosized Tio2

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.

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