Effects of Hydro and Hormonal Seed Priming on Seed Germination of Milk Thistle under Saline Stress Condition

Salinity is an abiotic stress which has harmful effects on germination of many plants. Therefore, high germination rate and vigorous early growth under salty soils is preferred. Seed priming is a way to increase salt tolerance of plants. An experiment was conducted to investigate the effect of seed priming on germination of milk thistle under salinity condition. The treatments were 4 levels of seed priming (no priming, distilled water as hydro priming and 0.5 and 1.0 mM salicylic acid) and 5 levels of salinity (0, 40 and 80 mM NaCl and 40 and 80 mM CaCl2). The experiment arranged as a factorial in a completely randomized design (CRD) with three replications. Results showed that salinity decreased germination percentage and germination rate to about 16 and 32% in 80 mM CaCl2 level compared to control, respectively. The highest mean germination time (5.7 day) were belonged to 80 mM CaCl2. Radicle and plumule length significantly decreased by 80 mM NaCl and 40 and 80 mM CaCl2. The lowest seedling weight and seed stamina observed in 80 mM CaCl2. 0.5 mM salicylic acid improved all traits except mean germination time as compared to control. Salicylic acid (0.5 mM) improved radicle length under 0, 40 and 80 mM NaCl salinity levels as well as increased plumule length at the 0 and 40 mM NaCl salinity conditions.

Download Full-text

Morphometry and Germination of Passovia pyrifolia, Struthanthus marginatus and Phoradendron mucronatum Diaspores

Planta Daninha ◽

10.1590/s0100-83582018360100010 ◽

2018 ◽

Vol 36 (0) ◽

Author(s):

J.M.L. AZEVEDO ◽

C.G.G. SANTOS ◽

C.S. CAIRES ◽

J.C. ARAÚJO NETO ◽

R.C. SOUZA

Keyword(s):

Moisture Content ◽

Germination Rate ◽

Germination Percentage ◽

Percentage Germination ◽

Essential Characteristic ◽

Germination Time ◽

Randomized Design ◽

Different Temperatures ◽

Mean Germination Time ◽

Completely Randomized Design

ABSTRACT: The goal of this work was to characterize morphometrically diaspores from three species of mistletoes (Passovia pyrifolia, Struthanthus marginatus e Phoradendron mucronatum), as well as to study its germination behavior according to temperature and luminosity. In germination tests, the completely randomized design was used, in factor scheme 4 x 2, with 4 replications of 15 diaspores. The factors for the first test were different temperatures and two diaspore conditions (intact and without epicarp). In the second test, factors were different light qualities and two diaspore conditions. The analyzed variables were: germination percentage, germination rate index (GRI) and mean germination time (MGT). Mistletoe’s diaspores present an elliptic shape with viscin layer, an essential characteristic for the dispersion of the species. In addition to this, they present a distinction as for the moisture content when compared to the Loranthaceae and Viscaceae, where the latter presents the highest content. Germination is greater at the alternated temperature of 20-30 oC for P. pyrifolia and P. mucronatum diaspores and 20 oC for S. marginatus. Luminosity interfered in the germination of P. mucronatum and S. marginatus but did not influence P. pyrifolia. The diaspore condition influenced the germination of the three studied species, observing a significant increase in their germination when the epicarp was removed. Temperature and luminosity interfere directly in the GRI and MGT of the three mistletoes species.

Download Full-text

Seed Quality of Sorghum [Sorghum Bicolor (L.) Moesch] as Affected By Seed Priming and Storage

NASS Journal of Agricultural Sciences ◽

10.36956/njas.v3i1.310 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Awale Degewione Shirdon ◽

Khalid Ibrahim Omer ◽

R.P.S Tommer ◽

Ketema Belete

Keyword(s):

Seed Quality ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Quality Parameters ◽

Storage Duration ◽

Germination Time ◽

Randomized Design ◽

Mean Germination Time ◽

Almost All

Sorghum is being produced primarily as a food grain throughout the world. It is one of the major traditional food crops in Ethiopia. However, the productivity of the crop is very low and poor seed quality is one of the major determinants. An experiment study was conducted in laboratory and glasshouse to evaluate the effect of seed priming on seed quality parameters of three sorghum varieties:(Muyra-I, Muyra-II and Fendisha) with different storage of one year and two years using different priming agents with different soaking time for each priming agent (1.0mM KNO3, 1mM NaCl and distilled water for 72, 48 and 12 hours respectively) in 2012 at Haramaya University Seed laboratory. Completely randomized design in 2 x 3 x 4 factorial arrangement with three replications was used to carry out the germination and vigor tests. The priming concentrations and durations were based on previous work, which was effective in hydro and osmo-conditioning of sorghum seed. In the experiment, seed priming increased germination percentage (GP), Mean Germination Time (MGT) and improved seedling shoot (SSL) and root length (SRL), seedling dry weight (SDW) and seedling vigor indices in all varieties of both storage durations compared to their respective control. Priming with NaCl and KNO3 showed positive response followed by hydropriming for all varieties of different storage duration. When treated with sodium chloride, almost all the varieties of each storage year demonstrated an increase in germination and the degree of increment was higher for Muyra-I(__) and Fendisha (___), the lowest being for Muyra-II which was significantly lower for both storage year. In case of mean germination time Fendisha exhibited better with hydropriming for older seeds (___) and with KNO3 for newer seeds(___) while Muyra-II and Muyra-I showed better with KNO3 (____) for older seeds and with hydropriming for newer seeds. Responses of varieties for the remaining parameters were intermediate. Therefore, sorghum seed priming with osmo-media and water can compensate storage deterioration of sorghum seeds in addition to significantly improving the seed germination and vigor quality parameters

Download Full-text

Phytotoxicity of Chitosan and SiO2 Nanoparticles to Seed Germination of Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) Plants

Notulae Scientia Biologicae ◽

10.15835/nsb9210075 ◽

2017 ◽

Vol 9 (2) ◽

pp. 242-249 ◽

Cited By ~ 5

Author(s):

Faride BEHBOUDI ◽

Zeinalabedin TAHMASEBI SARVESTANI ◽

Mohamad Zaman KASSAEE ◽

Seyed Ali Mohamad MODARES SANAVI ◽

Ali SOROOSHZADEH

Keyword(s):

Germination Rate ◽

Dry Weight ◽

Sio2 Nanoparticles ◽

Triticum Aestivum L ◽

Germination Percentage ◽

Hordeum Vulgare L ◽

Randomized Design ◽

Direct Exposure ◽

Contrast Exposure ◽

Mean Germination Time

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.

Download Full-text

Sensitivity of Seed Germination to Salt Stress in Teff [Eragrostis tef (Zucc.) Trotter]

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2019.0004 ◽

2019 ◽

Vol 76 (1) ◽

pp. 91

Author(s):

Panayiota PAPASTYLIANOU ◽

Ilias TRAVLOS ◽

Ioannis ROUSSIS ◽

Dimitrios BILALIS

Keyword(s):

Salt Stress ◽

Agricultural Development ◽

Animal Feed ◽

Moisture Stress ◽

Germination Percentage ◽

Limiting Factors ◽

Eragrostis Tef ◽

Germination Time ◽

Randomized Design ◽

Mean Germination Time

Teff is an annual grass originated in Ethiopia, grown for both human food and animal feed. Salinity and moisture stress are the main limiting factors of agricultural development on arid and semi-arid regions. The aim of this study was to investigate the effect of salinity due to NaCl on germination of teff. A set of experiments was conducted under a completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 25 °C) in the dark for 10 days. In order to create salt stress conditions during the germination process, seven solutions were used (0 as control, 80, 160, 240 and 320, 400 and 480 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The presence of NaCl reduced germination, especially above 240 mM for 15 °C and 400 mM for 25 °C. The mean germination time increased with higher NaCl levels.

Download Full-text

Sensitivity of Seed Germination to Temperature and Light in Helichrysum amorginum

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2020.0007 ◽

2020 ◽

Vol 77 (1) ◽

pp. 7

Author(s):

Sofia ARGYRIOU ◽

Panayiota PAPASTYLIANOU ◽

George STAVROPOULOS ◽

Angeliki KOUSTA ◽

Ioanna KAKABOUKI ◽

...

Keyword(s):

Seed Germination ◽

Seedling Survival ◽

Germination Percentage ◽

Effect Of Temperature ◽

Germination Time ◽

Randomized Design ◽

Perennial Shrub ◽

Mean Germination Time ◽

Lower Temperature ◽

Seed Propagation

Helichrysum amorginum is a perennial shrub, endemic on the Greek island of Amorgos and used for medicinal, cosmetic and ornamental purposes. Information concerning germination behavior and seedling survival is considered essential for the establishment and cultivation of wild species. The objective of this study was to investigate the effect of temperature and light on germination of Helichrysum amorginum seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at three different suboptimal germination temperatures (15, 20 and 25°C) in light and dark conditions for 10 days. Seed material was collected from two different fields of the island (Aegiali and Katapola). The germination performance was evaluated by final germination percentage and mean germination time. The effect of temperature on seed germination percentage and mean germination time was significant in all cases. The presence of light enhanced germination, especially at the optimum temperature of 20°C. In most cases the mean germination time increased at the lower temperature of 15°C. Seeds from Aegiali exhibited higher germination ability than the seed lot from Katapola. Knowledge of seed germination behaviour is of major importance in order to establish effective protocols for seed propagation in Helichrysum amorginum.

Download Full-text

Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

International Journal of Life Sciences ◽

10.3126/ijls.v9i1.11919 ◽

2015 ◽

Vol 9 (1) ◽

pp. 6-11

Author(s):

Abolfazl Masoumi Zavariyan ◽

Mojtaba Yousefi Rad ◽

Mohsen Asghari

Keyword(s):

Drought Stress ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Silybum Marianum ◽

Randomized Design ◽

Seedling Length ◽

Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

Download Full-text

Potasssium chloride as a nutrient seed primer to enhance salt‑tolerance in maize

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2012000800020 ◽

2012 ◽

Vol 47 (8) ◽

pp. 1181-1184 ◽

Cited By ~ 4

Author(s):

Badar‑uz‑Zaman ◽

Arshad Ali ◽

Syed Ishtiaq Hyder ◽

Muhammad Arshadullah ◽

Saqib Umar Bhatti

Keyword(s):

Salt Stress ◽

Salinity Stress ◽

Dry Matter ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

Stress Conditions ◽

Maize Crop ◽

Mean Germination Time ◽

Muriate Of Potash

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.

Download Full-text

Germination Response of MR 219 Rice Variety to Different Exposure Times and Periods of 2450 MHz Microwave Frequency

The Scientific World JOURNAL ◽

10.1155/2013/408026 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

Daryush Talei ◽

Alireza Valdiani ◽

Mahmood Maziah ◽

Mohammad Mohsenkhah

Keyword(s):

Seed Germination ◽

Root Length ◽

Germination Rate ◽

Rice Variety ◽

Microwave Frequency ◽

Germination Percentage ◽

Rice Seed ◽

Germination Time ◽

Primary Shoot ◽

Mean Germination Time

Germination is a key process in plants' phenological cycles. Accelerating this process could lead to improvment of the seedling growth as well as the cultivation efficiency. To achieve this, the effect of microwave frequency on the germination of rice seeds was examined. The physiological feedbacks of the MR 219 rice variety in terms of seed germination rate (GR), germination percentage (GP), and mean germination time (MGT) were analyzed by exposing its seeds to 2450 MHz of microwave frequency for one, four, seven, and ten hours. It was revealed that exposing the seeds to the microwave frequency for 10 hours resulted in the highest GP. This treatment led to 100% of germination after three days with a mean germination time of 2.1 days. Although the other exposure times of microwave frequency caused the moderate effects on germination with aGPa3ranged from 93% to 98%, they failed to reduce theMGTa3. The results showed that ten-hour exposure times of microwave frequency for six days significantly facilitated and improved the germination indices (primary shoot and root length). Therefore, the technique is expected to benefit the improvement of rice seed germination considering its simplicity and efficacy in increasing the germination percentage and rate as well as the primary shoot and root length without causing any environmental toxicity.

Download Full-text

Germination of Primed Seed under NaCl Stress in Wheat

ISRN Botany ◽

10.5402/2012/167804 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Michael P. Fuller ◽

Jalal H. Hamza ◽

Hail Z. Rihan ◽

Mohammad Al-Issawi

Keyword(s):

Salt Stress ◽

Germination Rate ◽

Arable Land ◽

Nacl Stress ◽

Germination Percentage ◽

Randomized Design ◽

Nacl Concentration ◽

Mean Germination Time ◽

Coefficient Of Velocity ◽

Germination Indices

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.

Download Full-text

Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

Agronomía Colombiana ◽

10.15446/agron.colomb.v33n3.53074 ◽

2016 ◽

Vol 34 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Fabio Ernesto Martínez M. ◽

Diego Miranda L. ◽

Stanislav Magnitskiy

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Positive Response ◽

Germination Rate ◽

Germination Percentage ◽

Annona Squamosa ◽

Germination Time ◽

Mean Germination Time ◽

Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

Download Full-text