scholarly journals Potasssium chloride as a nutrient seed primer to enhance salt‑tolerance in maize

2012 ◽  
Vol 47 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Badar‑uz‑Zaman ◽  
Arshad Ali ◽  
Syed Ishtiaq Hyder ◽  
Muhammad Arshadullah ◽  
Saqib Umar Bhatti
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Matter ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Maize Crop ◽  
Mean Germination Time ◽  
Muriate Of Potash

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.

scholarly journals The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Tomato Seeds ◽  
Germination Characteristics ◽  
Salinity Levels ◽  
Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

scholarly journals Germination of Primed Seed under NaCl Stress in Wheat

ISRN Botany ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Michael P. Fuller ◽  
Jalal H. Hamza ◽  
Hail Z. Rihan ◽  
Mohammad Al-Issawi
Keyword(s):  
Salt Stress ◽  
Germination Rate ◽  
Arable Land ◽  
Nacl Stress ◽  
Germination Percentage ◽  
Randomized Design ◽  
Nacl Concentration ◽  
Mean Germination Time ◽  
Coefficient Of Velocity ◽  
Germination Indices

Soil salinity affects a large and increasing amount of arable land worldwide, and genetic and agronomic solutions to increasing salt tolerance are urgently needed. Experiments were conducted to improve wheat seed performance under salinity stress conditions after priming. An experiment was conducted using a completely randomized design of four replications for germination indices in wheat (Triticum aestivum L. cv. Caxton). Normal and primed seed with PEG6000 at  MPa and five concentrations of NaCl (0, 50, 100, 150, and 200 mM) were tested. Results indicate that priming seed significantly () increased germination percentage at first count and final count, coefficient of velocity of germination, germination rate index, and mean germination time, while increasing of NaCl concentration significantly reduced it. Priming seed improved germination attributes at all NaCl concentration levels. The priming appeared to be able to overcome the effect of salt stress at 50 to 100 mM and reduce the effect of NaCl at higher concentrations up to 200 mM. The primed seed gave both faster germination and led to higher germination when under salt stress. We conclude that using priming techniques can effectively enhance the germination seed under saline condition.

scholarly journals Effects of Hydro and Hormonal Seed Priming on Seed Germination of Milk Thistle under Saline Stress Condition

2014 ◽  
Vol 6 (3) ◽  
pp. 374-380
Author(s):  
Yousef NASIRI ◽  
Parisa FEYZI ◽  
Abdollah JAVANMARD
Keyword(s):  
Salicylic Acid ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Milk Thistle ◽  
Saline Stress ◽  
Nacl Salinity ◽  
Germination Time ◽  
Randomized Design ◽  
Mean Germination Time

Salinity is an abiotic stress which has harmful effects on germination of many plants. Therefore, high germination rate and vigorous early growth under salty soils is preferred. Seed priming is a way to increase salt tolerance of plants. An experiment was conducted to investigate the effect of seed priming on germination of milk thistle under salinity condition. The treatments were 4 levels of seed priming (no priming, distilled water as hydro priming and 0.5 and 1.0 mM salicylic acid) and 5 levels of salinity (0, 40 and 80 mM NaCl and 40 and 80 mM CaCl2). The experiment arranged as a factorial in a completely randomized design (CRD) with three replications. Results showed that salinity decreased germination percentage and germination rate to about 16 and 32% in 80 mM CaCl2 level compared to control, respectively. The highest mean germination time (5.7 day) were belonged to 80 mM CaCl2. Radicle and plumule length significantly decreased by 80 mM NaCl and 40 and 80 mM CaCl2. The lowest seedling weight and seed stamina observed in 80 mM CaCl2. 0.5 mM salicylic acid improved all traits except mean germination time as compared to control.  Salicylic acid (0.5 mM) improved radicle length under 0, 40 and 80 mM NaCl salinity levels as well as increased plumule length at the 0 and 40 mM NaCl salinity conditions.

scholarly journals Effect of Different Priming Methods on Germination and Seedling Establishment of Two Medicinal Plants under Salt Stress Conditions

2015 ◽  
Vol 48 (3) ◽  
pp. 43-51 ◽  
Author(s):  
O. Younesi ◽  
A. Moradi
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Medicinal Plants ◽  
Salinity Stress ◽  
Seedling Establishment ◽  
Growth Traits ◽  
Germination Rate ◽  
Stress Conditions ◽  
Metabolic Reaction ◽  
Lemon Balm

Abstract Priming is one of the seed enhancement methods that might be resulted in increasing seed performance (germination and emergence) under stress conditions, such as salinity. Salinity is a major environmental stress which adversely affects germination and seedling establishment in a wide variety of crops. The experiment was arranged as a factorial in completely randomized design (CRD) at Seed Research Laboratory of College of Agriculture, University of Tehran, Iran. The objective of this research was to evaluate the effect of different priming methods on seed germination of two medicinal plants including lemon balm (Melissa officinalis L.) and cumin (Cuminum cyminum L.) under salinity stress. Treatments were combinations of two levels of salinity stress (0 and 10 dsm−1) and five levels of priming (control = non-priming), GA3, manitol, NaCl and distilled water) with three replications. Seeds of lemon balm and cumin were primed for 24 h at 25°C. Results revealed that different growth traits (including germination percentage, germination rate, seedling dry weight, plumule and radical length) significantly (p=0.05) decreased with applying salinity. However, priming of seeds with different materials particularly GA3 was useful for alleviating salt stress effects and improving germination and seedling establishment under salt stress. Under salinity condition, primed seeds possessed more germination and emergence than control. The result of this experiment is consistent with the hypothesis that under salinity stress, priming can prepare a suitable metabolic reaction in seeds and can improve seed germination performance and seedling establishment.

scholarly journals Seed Priming to Overcome Salinity Stress in Persian Cultivars of Alfalfa (Medicago sativa L.)

2015 ◽  
Vol 7 (1) ◽  
pp. 96-101 ◽  
Author(s):  
Ali SEPEHRI ◽  
Saeed NAJARI ◽  
Hossein Reza ROUHI
Keyword(s):  
Salinity Stress ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Stress Index ◽  
Randomized Design ◽  
Vigor Index ◽  
Salinity Levels ◽  
Seedling Germination ◽  
Alfalfa Varieties

In order to investigate the effect of hydro-priming on seed germination with distilled water on germination of five Alfalfa cultivars under salinity stress, an experiment was conducted as a factorial experiment based on a completely randomized design with three replications. Seven levels of hydro-priming and salinity of NaCl including prime and non-salinity, prime and 50 mM salinity, prime and 100 mM salinity, prime and 150 mM salinity, prime and 200 mM salinity, prime and 250 mM salinity and without prime and salinity and five alfalfa varieties, including ‘Hamedani’, ‘Isfahani’, ‘Bami’, ‘Yazdi’ and ‘Ghareh Yonjeh’ were used. The results showed that the main effect of prime, salinity and cultivars and their interaction in all studied traits were significantly affected at the 5% probability level. Priming treatments in non-salinity of all cultivars were the highest. In all cultivars, final germination percentage, length and weight of radicle, plumule and seedling, germination rate and time, relative radicle elongation, vigor index and stress index, were significantly improved in response to priming in salinity levels of 50-200 mM, compared to control. Radicle produced higher length and weight than the plumule in hydro- priming and salinity treatments. ‘Hamedani’ cultivar in most of studied characteristics had a better response than others. The lowest response to salinity stress and priming was observed in ‘Yazdi’ cultivar.

Nutrient Priming in Different Maize Cultivars and Evaluation of Vigour Improvement Under Controlled Conditions

2017 ◽  
Vol 60 (2) ◽  
pp. 65-69
Author(s):  
Adnan Umair ◽  
Muhammad Abid ◽  
Ijaz Ali ◽  
Kashif Bashir ◽  
Waqas Naseem ◽  
...  
Keyword(s):  
Dry Matter ◽  
Seed Priming ◽  
Germination Percentage ◽  
Plant Vigour ◽  
Maize Cultivars ◽  
Matter Production ◽  
Germination Characteristics ◽  
Vigour Index ◽  
Mean Germination Time ◽  
Crop Stand

Laboratory and pot experiments were conducted to study the effect of seed priming on vigourand germination characteristics of different maize cultivars during the year 2011. The seeds of four maizecultivars viz. EV 7004Q, Islamabad Gold, Rakaposhi and Sohan-3 were primed using 0% P (dry seededcontrol), 0.6% P and 1.2% P solutions of KH2PO4 for 16 h. The experiment was laid out in CompleteRandomized Design (CRD) having three replicates. All the seed priming treatments significantly improvedthe plant vigour in terms of enhanced germination percentage, reduced mean germination time (MGT),improved root, shoot lengths and dry matter production. Among the four cultivars of maize the IslamabadGold performed best followed by Sohan-3. Highest germination (94.57%) and vigour index (VI) of 431.66was observed in Islamabad Gold where 1.2% P applied compared to dry seeded control. Nutrient seedpriming may be used to improve germination vigour and crop stand establishment under field conditionsleading to good yield targets in maize under rainfed conditions.

Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress

2007 ◽  
Vol 58 (8) ◽  
pp. 811 ◽  
Author(s):  
S. Zhang ◽  
J. Hu ◽  
Y. Zhang ◽  
X. J. Xie ◽  
Allen Knapp
Keyword(s):  
Salt Stress ◽  
Medicago Sativa ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Dry Weight ◽  
Medicago Sativa L ◽  
High Level

Salt stress is an important constraint to lucerne (Medicago sativa L.) production in many parts of the world. Seeds of 3 lucerne varieties, cvv. Victoria, Golden Empress, and Victor, were used to investigate the effects of seed priming with 5 µm/L brassinolide on germination and seedling growth under a high level of salt stress (13.6 dS/m NaCl solution). The results showed that germination percentage, germination index, and vigour index of lucerne seeds primed with brassinolide were significantly higher than those of the non-primed seeds under salinity stress in each variety. Seed priming with brassinolide significantly increased the shoot fresh weight, shoot dry weight, and root dry weight in 2 varieties, and significantly increased the root length and root vigour in each variety. It also significantly increased the activities of antioxidant enzymes, peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), in Victoria and Victor seedlings. During seedling growth, the primed seeds significantly reduced the malondialdehyde (MDA) accumulation. This suggests that priming lucerne seed with brassinolide at a suitable concentration can improve germination and seedling growth under high-saline soils.

Effect of exogenous nitric oxide (NO) supply on germination and seedling growth of mungbean (cv. Nm-54) under salinity stress

2017 ◽  
Author(s):  
Muhammad Salahuddin ◽  
Fahim Nawaz ◽  
Muhammad Shahbaz ◽  
Muhammad Naeem ◽  
Bilal Zulfiqar ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Seedling Growth ◽  
Dry Matter ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Tolerance Index ◽  
Fresh Weight ◽  
Chlorophyll Contents ◽  
Exogenous Nitric Oxide

The present study evaluated the effects of exogenous NO supply, using sodium nitroprusside (SNP) as a source of NO, on germination and seedling growth of mungbean (Vigna radiata) under salt stress conditions. The results showed that the seeds treated with NO solution (0.2 mM SNP) exhibited 80% and 109% higher germination percentage and germination stress tolerance index (GSI) than untreated seeds (control) under salt stress conditions. Similarly, the seedlings fertigated with NO maintained the highest values of 77.8%, 84.3%, 77.2%, 60.5% and 100.3% for plant height stress tolerance index (PHSI), root length stress tolerance index (RLSI), shoot fresh weight stress tolerance index, root fresh weight stress tolerance index (RFSI) and dry matter stress tolerance index (DMSI), respectively. Moreover, the NO fertigated seedlings maintained 57% higher chlorophyll contents than control seedlings. It is concluded that exogenous NO supply is an effective approach to ensure uniform stand establishment in saline regions of the world.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

2020 ◽  
pp. 1-9
Author(s):  
Keum-Ah Lee ◽  
Youngnam Kim ◽  
Hossein Alizadeh ◽  
David W.M. Leung
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Salinity Stress ◽  
Lolium Multiflorum ◽  
Casein Hydrolysate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Italian Ryegrass ◽  
Significant Difference ◽  
Protein Amino Acids

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Sign in / Sign up

Export Citation Format

Share Document