Effect of Estrus Induction on Blood Biochemical and Mineral Profiles and Fertility Rate in Retained Fetal Membranes Affected and Normally Calved Riverine Buffaloes

Author(s):  
M. Selvaraju ◽  
K. Ganesh

Background: Calving to conception interval is abnormally extended by the occurrence of retained fetal membranes (RFM) by altering the blood biochemical and mineral milieu in cows. Hence this experiment was conducted in RFM affected and normally calved (NC) buffaloes by inducing estrus with CIDR plus PGF2α protocol to study the blood biochemical and mineral profiles and to correlate them with fertility rate. Methods: Buffaloes (n=64) at 45-60 days postpartum including 32 treated for RFM and 32 NC from field were equally divided into groups I and II and groups III and IV, respectively. Buffaloes of groups I and III were initially dewormed and administered 35-50 g mineral mixture daily orally for 15 days in the concentrate feed. Then, buffaloes of all the groups were treated with CIDR plus PGF2α. After CIDR removal, all the buffaloes were artificially inseminated twice at 48 and 72 hrs. Blood was collected during different stages of treatment from all the buffaloes to assess the blood biochemical and mineral status. The animals returned to estrus following FTAI were again inseminated during subsequent estrus. Pregnancy diagnosis was done at 60 days post-AI and conception rates for induced estrus and overall of two cycles were calculated.Result: In all the groups, blood glucose, total protein, triglycerides, cholesterol and phosphorus levels increased from the time of selection to 10 days post-AI. There was an altered calcium phosphorus ratio in RFM affected buffaloes (1:1) at the time of selection. CIDR plus PGF2α protocol influenced the blood biochemical constituents and brought the calcium and phosphorus ratio as 2:1 and improved the fertility in riverine buffaloes. The overall conception rate was 62.50, 37.50, 87.50 and 75.00 per cent, in group I, II, III and IV buffaloes, respectively.

1983 ◽  
Vol 63 (3) ◽  
pp. 699-705 ◽  
Author(s):  
J. O. ATTEH ◽  
S. LEESON

The influence of supplementing pig diets with 0, 5 or 10% fat, increasing dietary calcium and available phosphorus levels from 0.8 to 1.2% and from 0.53 to 0.8%, respectively, was investigated with 3-wk-old weaner pigs for a period of 6 wk. Average daily gain (P < 0.05) and feed intake (P < 0.01) were reduced with dietary fat supplementation. However, 10% supplemental fat in the diet caused an improvement (P < 0.05) in feed:gain ratio. Dietary calcium and phosphorus levels had no significant effect on daily weight gain, feed intake or feed:gain ratio. There was no significant effect of dietary fat or calcium and phosphorus treatment on digestibility of calcium, phosphorus or magnesium. There was also no significant difference in the pH of stomach and duodenal contents due to the dietary treatments. The rectal contents of pigs fed supplemental fat was found to be more acidic than those from the control group (P < 0.05). There was no significant effect of dietary fat, calcium and phosphorus treatments on bone or serum calcium, phosphorus or magnesium content. It is concluded that weaner pigs will tolerate high levels of fat without any detrimental effect on mineral digestibility and subsequent bone mineralization. Key words: Weaner pigs, fat, calcium, performance, bone and serum minerals


2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Dede Sukandar ◽  
Anna Muawanah ◽  
Eka Rizki Amelia ◽  
Widad Basalamah

Abstrak Tepung sukun (Artocarpus communis) merupakan tepung yang bebas gluten sehingga baik digunakan sebagai alternatif dalam pembuatan cookies untuk anak penderita autis. Penelitian ini bertujuan mengetahui tingkat kesukaan panelis terhadap cookies sukun dan berbagai formulasinya dibandingkan dengan cookies berbahan dasar tepung lain yang meliputi pengaruh penambahan bahan tambahan terhadap sifat kimia, fisika, daya terima cookies sukun, kadar kalsium dan fosforus cookies sukun, dan mengetahui cookies sukun tersukai memenuhi standar mutu cookies menurut SNI 01-2973-1992 atau tidak. Uji organoleptik dilakukan untuk mengetahui tingkat kesukaan dan penerimaan panelis terhadap cookies sukun dibandingkan dengan cookies berbahan dasar tepung lain (terigu, beras, sagu) dan cookies sukun dalam berbagai formulasi. Parameter yang digunakan meliputi warna, aroma, tekstur, rasa dan penerimaan keseluruhan. Uji kadar kalsium dilakukan menggunakan spektroskopi serapan atom pada λ 422.7 nm dan kadar fosforus menggunakan spektroskopi UV-Vis pada λ 880 nm. Data yang diperoleh dianalisis menggunakan analisis sidik ragam anova dan analisis Duncan. Cookies sukun memiliki penerimaan yang rendah dibandingkan cookies tepung lain berdasarkan penerimaan rasa dan penerimaan umum. Formulasi cookies sukun memperbaiki sifat fisik (aroma, rasa, warna, dan tekstur) dan daya terima cookies sukun oleh panelis. Cookies sukun tersukai adalah formulasi 718 dengan bahan tambahan susu kedelai.  Mutu cookies sukun tersukai formulasi 718 sesuai dengan SNI 01-2973-1992 kecuali kadar protein yang masih rendah 8.05% dan terdapat kandungan tembaga dengan sebesar 1.56 ppm. Cookies sukun tersukai memiliki kadar kalsium dan fosforus tertinggi dibandingkan dengan tepung sukun dan cookies komersil untuk anak penderita autis sehingga cookies sukun tersukai sangat baik dikonsumsi oleh anak penderita autis. Kata kunci : Artocarpus communis, autis, cookies sukun, organoleptik, kalsium, fosforus Abstract Breadfruit (Artocarpus communis) has been reported as a functional food ingredient for autistic children. This study aims to determine the level of A panelist on cookies breadfruit and various formulations compared to other flour-based cookies which include the effect of additives on the properties of chemistry, physics, power accept cookies breadfruit, calcium and phosphorus levels cookies breadfruit, breadfruit cookies and find out the most preferred if it meets quality standards according to SNI 01-2973-1992 cookies or not. Organoleptic test was conducted to determine the level of preference and acceptance panelist on cookies breadfruit compared with other flour-based cookies     (wheat, rice, sago) and breadfruit cookies in a variety of formulation. The parameters used include, colour, frageance, texture, flavor and overall acceptance. Calcium level test performed using atomic absorption spectroscopy method at λ 422.7 nm and phosphorus levels using UV-Vis spectroscopy at λ 880 nm. The data were analyzed using analysis of variance anova and Duncan analysis. The test results showed Cookies breadfruit organoleptif have low enrollment compared to other flour cookies views of the average revenue flavor and general acceptance of cookies breadfruit, breadfruit cookies formulations can improve the physical properties (fragrance, flavor, color, and texture) and acceptance of cookies breadfruit by panelists. An increase in the average value of A formulation compared breadfruit cookies before formulation. Cookies are breadfruit tersukai 718 formulations with soy milk additives. Quality breadfruit cookies tersukai 718 formulations in general accordance with SNI 01-2973-1992 except protein content and contained 8.05% Cu content with a small concentration (1.56 ppm). Cookies breadfruit tersukai have the highest levels of calcium and phosphorus compared with breadfruit flour and commercial cookies for autistic children so that the most preferred breadfruit cookies very well taken by autistic children. Keywords : Artocarpus communis, autism, breadfruit cookies, organoleptic, calcium, phosphorus


bionature ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Amiruddin Ahmad ◽  
Muh. Jurhadi Kadir

Abstract. This study aims to determine the use of pandan leaf extracts to evaluate the levels of calcium and phosphorus of salted duck eggs. The results showed that the effect of the addition of leaf extracts on the evaluation of calcium levels and phosphorus levels in salted duck eggs with different concentrations, did not have a significant effect or had no significant effect (P> 0.05) on calcium content, T0 treatment was not significantly different from T1, T2 and T3 and vice versa. even so but it still has an increase in calcium levels. Treatment of calcium levels from lowest to highest are T1 (0.15%), T0 (0.16%), T3 (0.17%) and T2 (0.17%). Phosphorus levels in salted duck eggs given pandan leaf extract with different concentrations have a very significant effect (P <0.05) on the phosphorus content, T0 treatment is significantly different from T1, T2, and T3, T1 is significantly different from T0 and T3 but not significantly different from T2, T2 is significantly different from T0 but not significantly different from T1 and T3, T3 is significantly different from T0 and T1 but not significantly different from T2. even so but it still has an increase in calcium levels. Calcium levels from lowest to highest are T0 (0.19%), T3 (0.23%), T2 (0.23%) and T1 (0.26%). The conclusion is that the treatment of the addition of fragrant pandan leaf extract can not affect calcium levels but affect the levels of phosphorus salted duck eggs. The best treatment is T3 treatment (30% pandanus fragrant leaf extract) with a value of 0.16 calcium levels and a value of phosphorous content 0.22. Keywords: Salted eggs, pandan leaves, calcium, phosphorus.


2014 ◽  
Vol 68 (1-2) ◽  
pp. 31-42
Author(s):  
Milan Adamovic ◽  
Horea Samanc ◽  
Danijela Kirovski ◽  
Ivan Vujanac ◽  
Olivera Valcic

The objective of the work was to investigate the influence of partial substitution of magnesium oxide with natural bentonite in feed mixtures used in feeding of cows during their exposure to heat stress. The investigation lasted 30 days and was carried out during last ten days of may and first twenty days of June when average air temperature in stables was 36.6?2.5oC. In the experiment there were included 30 cows of Holstein breed in first phase of lactation, which were divided into two groups of 15 cows: control (C) and experimental (E). Group C was fed with experimental mineral mixture that contained 60% of magnesium oxide during the whole investigation period. Group E was fed with experimental mineral mixture that contained 40% of magnesium oxide as well as 20% of natural bentonite. Remaining ingredients in both control and experimental mineral mixtures were the same and also contained 20% of sodium bicarbonate and 20% of zeolite in the same quantities. The control and experimental mineral mixtures were mixed into complete feed mixture (18% UP) in the amount of 1%. At tne end of the investigation period, on the 30th day, there were taken samples of rumens contents for determining pH, and after that blood samples, in which, after the separation of blood serum, were determined glucose concentration, total proteins, albumin, globulin, urea, HDL cholesterol, LDL cholesterol, total bilirubin, calcium and phosphorus, as well as the activity of ALT and AST. By computation there was calculated the ratio between albumin and globulin, ALT and AST, and the ratio between calcium and phosphorus. Daily allowance and milk chemical composition ( percentage of fat, proteins and dry substance) were determined at the end of the investigation period, that is on the 30th day of lactation, for each cow individually. Partial substitution of magnesium oxide with bentonite influenced milk production increase, but it was statistically insignificant. Besides that, in E group of cows, percentage of fat and dry substance in milk was significantly increased (p<0.05 and 0.01 respectively), while percentage of protein increase was insignificant. The substitution of magnesium oxide with bentonite had no impact on the values of examined parameters of metabolic profile, but it led to statistically significant increase of rumen contents pH values (p<0.05). From the obtained results it can be concluded that substitution of magnesium oxide with bentonite in feed mixtures that are used for feeding cows during summer period, can prevent rumen acidosis, which high yielding cows incline to under the conditions of elevated external temperatures. Besides that, this kind of substitution leads to improvement of milk composition, especially when percentage of fat and dry substance in milk is concerned.


2015 ◽  
Vol 49 (6) ◽  
Author(s):  
B. L. Sharma ◽  
V. K. Bhatt ◽  
S. K. Jain ◽  
S. N. Shukla ◽  
M. K. Shukla

Profile of blood glucose, calcium, phosphorus and magnesium was studied during peri -parturient period <italic>i.e.</italic> on day 10 antepartum/ day of prolapse, day of parturition and day 10 postpartum in 12 normal pregnant buffaloes and 12 buffaloes with antepartum cervico vaginal prolapse during the last month of gestation. The blood glucose, serum calcium, phosphorus and magnesium concentration (mg/dl) in normal pregnant buffaloes were 62.13±1.01, 8.75±0.2, 6.04±0.12 and 1.7±0.10 on day 10 antepartum, 57.39±1.13, 8.30±0.25, 5.59±0.16 and 1.95±0.09 on day of parturition and 59.69±1.22, 6.69±0.23, 4.89±0.07 and 2.08±0.14 at day 10 postpartum respectively, where as, the corresponding values in buffaloes with antepartum cervicovaginal prolapse were 53.66±1.39, 3.99±0.11, 2.88±0.08 and 2.7±0.1 on the day of prolapse; 52.15±1.03, 6.77±0.29, 4.72±0.12 and 2.12±0.08 on the day of parturition; 57.63±1.28, 5.40±0.22, 4.29±0.14 and 1.89±0.14 on day 10 postpartum. The blood glucose, calcium and phosphorus concentration was significantly lower (p< 0.05) in buffaloes with antepartum cervico -vaginal prolapse as compared to normal pregnant buffaloes whereas, on day 10 antepartum/ day of prolapse the serum magnesium concentration was significantly higher in buffaloes with antepartum prolapse as compared to normal pregnant buffaloes.


2021 ◽  
Author(s):  
Marcelo Sebastião Rezende ◽  
BELCHIOLINA BEATRIZ FONSECA ◽  
Paula Fernanda Sousa Braga ◽  
Ednaldo Carvalho Guimarães ◽  
Antonio Vicente Mundim

Abstract This study aimed to evaluate the influence of age and sex on the blood biochemical constituents of broiler breeders during the egg production stage. The analysis was performed in an industrial broiler breeder farm, and blood samples were collected from males and females at five different ages. At most ages, females had higher values of total proteins, albumin, globulins, triglycerides, cholesterol, glucose, calcium, phosphorus, Ca/P (calcium and phosphorus)ratio and gamma-glutamyltransferase (GGT). The values of uric acid, aspartate aminotransferase (AST), creatine kinase (CK), and alkaline phosphatase (PAL) were higher in males. The lowest protein values were found at 28 and 60 weeks old. The mean albumin values were significantly higher at 44 and 52 weeks old in males and females. A trend of increasing globulin values ​​with increasing age up to 52 weeks old was observed. Although calcium and phosphorus did not vary according to age, the Ca/P ratio was lowest at 36 weeks old. Comparing the means of both sexes, the AST and GGT values were significantly higher in 60-week-old birds. The highest serum levels of CK occurred at 28 and 52 weeks old. The physiological levels of serum biomarkers presented in this work are important for evaluating productivity performance, welfare, and disease indication in breeding flocks.


1995 ◽  
Vol 268 (2) ◽  
pp. E312-E317
Author(s):  
D. C. Hermey ◽  
R. A. Ireland ◽  
J. E. Zerwekh ◽  
S. N. Popoff

The osteopetrotic (op) rat is a lethal mutation characterized by severe skeletal sclerosis resulting from reduced bone resorption. Although the skeletal manifestations have been studied extensively, little is known about mineral homeostasis in this mutation. This paucity of data prompted us to undertake this study quantitating circulating levels of calcium, phosphorus, 1,25-dihydroxyvitamin D [1,25(OH)2D] and parathyroid hormone (PTH) in op mutants and normal rats between 2 and 8 wk of age. Calcium and phosphorus levels were significantly lower in op mutants at younger ages; both parameters normalized by 6 wk. Serum levels of 1,25(OH)2D were markedly elevated in op rats at all ages and showed no signs of normalization. Serum PTH levels were also elevated at most ages, with the greatest increase occurring when op mutants were severely hypocalcemic. These results demonstrate that, in op mutants, changes in circulating PTH and calcium levels were interdependent; however, levels of 1,25(OH)2D did not change despite normalization of serum calcium and phosphorus. The latter deserves further investigation and supports the hypothesis of a localized (skeletal) resistance to 1,25(OH)2D.


2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.


2019 ◽  
Vol 15 (01) ◽  
pp. 40-44 ◽  
Author(s):  
P M Joshi ◽  
D Patel ◽  
P D Patel

An on-farm trial for 90 days was conducted at four tribal villages to assess the effect of chelated mineral mixture (CMM) supplementation on blood biochemistry as well as hormonal and mineral status, nutrient intake and reproductive performance of the repeat breeder buffaloes (n = 24). The animals selected were randomly divided into two groups of 12 animals each. The group T1 was control group (farmer’s feeding schedule), and T2 treatment group (T1 + CMM @ 50 g/animal/day). Blood collection was done at 0, 45 and 90 days of the experiment from both the groups for assessment of blood biochemical, hormonal and mineral status of the animals. Average DM, DCP and TDN intake in repeat breeder buffaloes calculated as per information collected from farmers were statistically similar among both the groups and were as per ICAR requirements of animals. Results revealed significant (p less than 0.05) improvement in hemoglobin, serum glucose, total protein, total cholesterol, progesterone, and macro (Ca, P)–micro (Zn, Fe, Cu, Co, Mn) mineral status of chelated mineral supplemented group as compared to control animals. The number of days taken for a successful conception in repeat breeder buffaloes as well as the cost of feeding was also reduced upon supplementation of CMM in T2 as compared to T1 group. The control group recorded a 22.12% higher cost of rearing than the treatment group during the experimental period. The findings revealed the beneficial role of cheated minerals supplementation in improving health, nutritional and reproductive status of repeat breeding buffaloes.


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