scholarly journals THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Hamidah ◽  
Salni ◽  
Nina Tanzerina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Active Compound ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Active Compounds ◽  
Apple Leaves ◽  
And Staphylococcus Aureus

Escherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 µg/mL for Escherichia coli and 500 µg/mL for  Staphylococcus aureus. The minimum  inhibitory concentration of the active  compound  to  Escherichia  coli  and  Staphylococcus  aureus  in  500  µg/mL.  The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin.

scholarly journals Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals PENGARUH PEMANASAN, TINGKAT KEASAMAN (pH) DAN KONSENTRASI GARAM TERHADAP STABILITAS ANTI BAKTERI EKSTRAK ETANOL KULIT KAYU AKWAY (Drimys piperita Hook f.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba ◽  
Nitia Nilawati ◽  
Eka Syartika
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Heating Time ◽  
Diffusion Method ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus ◽  
Agar Well Diffusion Method

ABSTRACT  Akway (Drimys piperita) is a woody, evergreen and aromatic plant that belongs to family winteraceae. This plant is used by Sougb tribe lived in Sururey village, District of Anggi, to enhance the vitality of body. The objectives of the research were to determine antimicrobial stability of akway bark extracts influenced by heating time of 100OC, levels of acidity (pH) and salt contents.. Antimicrobial assays were done by using agar well diffusion method against four species of bacteria, i.e.  Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. The results showed that ethanolic extracts of akway bark only inhibited growth of B. cereus and S. aureus with minimum inhibitory concentration 0,99% and 0,89% . The levels of concentration and acidity of ethanol extracts  influenced the antimicrobial capacity of extracts.. Whereas heating time on 100OC during 25 minutes and salt contents  up to 5% of extract solution did not influence the antimicrobial stability of  akway bark extracts. Key words : akway, extracts, antimicrobe, pH, Heating, salt ABSTRAK Akway (Drimys piperita) adalah tumbuhan berkayu, aromatik dan hijau sepanjang tahun dan tergolong dalam suku winteraceae. Tumbuhan ini digunakan oleh Suku Sougb yang bermukim di desa Sururey Distrik Anggi, untuk mengobati malaria dan meningkatkan vitalitas tubuh. Tujuan dari penelitian ini adalah untuk menentukan stabilitas antimikroba ekstrak kulit kayu akway pada waktu pemanasan ekstrak pada 100OC, tingkat keasaman (pH) dan kandungan garam. Pengujian antimikroba dilakukan dengan menggunakan metode difusi agar terhadap empat spesies bakteri yaitu Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923. Hasil menunjukkan bahwa ekstrak etanol kulit kayu akway hanya dapat menghambat bakteri Bacillus cereus dan Staphylococcus aureus dengan konsentrasi hambat minimum masing-masing adalah 0,99% dan 0,89%. Tingkat konsentrasi dan keasaman (pH) mempengaruhi kapasitas antimikroba ekstrak etanol kulit kayu akway. Sedangkan perlakuan pemanasan pada suhu 100OC dengan lama pemanasan sampai dengan 25 menit dan penambahan garam NaCl sampai konsentrasi 5%  tidak berpengaruh pada stabilitas antimikroba ekstrak etanol kulit kayu akway.  

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

scholarly journals Antibacterial Activity of Murrayaquinone A and 6-Methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Biswanath Chakraborty ◽  
Suchandra Chakraborty ◽  
Chandan Saha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Naturally Occurring

The antibacterial activity of Murrayaquinone A (10), a naturally occurring carbazoloquinone alkaloid, and 6-methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione (11), a synthetic carbazoloquinone, both obtained during the development of the synthesis of Carbazomycin G, having unique quinone moiety, was studied against Gram-positive (Bacillus subtilisandStaphylococcus aureus) and Gram-negative (Escherichia coliandPseudomonassp.) bacteria. Compound10showed antibacterial activities against both ofEscherichia coliandStaphylococcus aureuswhereas compound11indicated the activity againstStaphylococcus aureusonly. Both compounds10and11exhibited minimum inhibitory concentration (MIC) of 50 μg mL−1againstStaphylococcus aureus.

scholarly journals Antibacterial Activity of Different Extract of Medicinal Plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (2) ◽  
pp. 51-56
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 56-63
Author(s):  
Julia Nanda Puspita ◽  
Rikhsan Kurniatuhadi ◽  
Rahmawati Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Umbi Batang Tumbuhan Sarang Semut (Myrmecodia Pendens Merr. & L. M. Perry) Terhadap Pseudomonas Aeruginosa Dan Staphylococcus Aureus

2017 ◽  
Author(s):  
Ardianti Febriana
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Phytochemical Screening ◽  
Gram Positive ◽  
Tetracycline Hcl ◽  
And Staphylococcus Aureus

AbstrakTumbuhan sarang semut (Myrmecodia pendens Merr. & L. M. Perry) secara empiris digunakan sebagai obat yang memiliki aktivitas antibakteri. Namun, belum pernah diteliti mengenai aktivitas antibakteri tersebut. Penelitian ini dilakukan untuk mengetahui aktivitas antibakteri umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) terhadap bakteri Gram negatif dan Gram positif yaitu Pseudomonas aeruginosa dan Staphylococcus aureus, menentukan konsentrasi hambat tumbuh minimum (KHTM), dan nilai banding ak- tivitas antibakterinya terhadap tetrasiklin-HCl. Hasil pengujian aktivitas antibakteri umbi batang sarang semut menunjukkan bahwa ekstrak umbi batang sarang semut memiliki ak- tivitas antibakteri terhadap kedua bakteri tersebut. Konsentrasi hambat tumbuh minimum (KHTM) ekstrak umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) terhadap Pseudomonas aeruginosa terletak pada konsentrasi 0,83 % , sedangkan pada Staph- ylococcus aureus terletak pada konsentrasi 0,8 %. Hasil uji banding ekstrak umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) dengan tetrasiklin-HCl terhadap Pseudomonas aeruginosa dan Staphylococcus aureus berturut-turut sebesar 1 : 6,709 x 10-4 dan 1 : 1,038 x 10-5. Penapisan fitokimia menunjukkan bahwa ekstrak umbi batang sarang semut (Myrmecodia pendens Merr. & L. M. Perry) mengandung flavonoid dan polifenol.Kata kunci: Myrmecodia pendens Merr. & L. M. Perry, Aktivitas antibakteri, Pseudomo- nas, StaphylococcusABSTRACTThe ant plant (Myrmecodia pendens Merr. & L. M. Perry) is one of plant applied as tradi- tional medicine. Empirically, the tuber of the ant plant have antibacterial activity. However, the examination of antimicrobial activity from extract of the tuber has never been reported. The research was done to know the activity of antimcrobial againts Gram positive and Gram negatif bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus, to determine Minimum Inhibitory Concentration (MIC) of the tuber, and comparative value antibacterial activity with tetracycline-HCl. The result revealed that the tuber of the ant plant (Myrmeco-dia pendens Merr. & L. M. Perry) has antibacterial activity againts those bacteria. The Min- imum Inhibitory Concentration of extract from the tuber of ant plant to Pseudomonas aeru- ginosa was in range 8,2x103-8,3x103 ppm of concentration, and to Staphylococcus aureus was in range 7,5x103-8x103 ppm of concentration. Comparative value of extract from the tuber of ant plant with tetracycline-HCl againts Pseudomonas aeruginosa and Staphylococ- cus aureus were respective 1:.6.709 x 10-4 and :1.038 x 10-5. Phytochemical screening showed that the extract of the tuber contains flavonoid and polifenol.Keywords: Myrmecodia pendens Merr. & L. M. Perry, Antibacterial activity, Pseudomonas, Staphylococcus

scholarly journals Chemical Composition and Antibacterial Activity of Cupressus dupreziana A. Camus

2007 ◽  
Vol 2 (9) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Messaoud Ramdani ◽  
Oualida Rached ◽  
Hocine Laouer ◽  
Meriem El Koli ◽  
Takia Lograda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Diffusion Method ◽  
Germacrene D ◽  
And Staphylococcus Aureus

The essential oils obtained by hydrodistillation from the green branchlets of Cupressus dupreziana A. Camus growing wild in Tassili n'Ajjer (Algeria) were analyzed by GC and GC/MS. The main constituents of the essential oils were α-pinene (27.2% - 44.2%), germacrene D (16.2% - 27.2%) and Δ3-carene (14.2% - 26.7%). The oils showed insignificant activity on the growth of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923), when investigated by the diffusion method.

scholarly journals POTENSI ANTIBAKTERI BINTANG LAUT DARI PERAIRAN PANTAI KELURAHAN TONGKAINA MANADO

2019 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Delpris Piter ◽  
Esther D Angkouw ◽  
Fitje Losung
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Agalactiae ◽  
Antibacterial Agents ◽  
Sea Water ◽  
Diffusion Method ◽  
E Coli ◽  
Linckia Laevigata ◽  
And Staphylococcus Aureus

Starfish is one of the species of the asteroidean class, and is grouped into phylum echinoderms. Echinoderms consist of approximately 6000 species and all live in sea water. Starfish is one source bioactive compounds. Active compounds from starfish have been known to have activities antioxidant, antibacterial, anti-inflammatory, antifungal and immunostimulatory, there are also blue starfish that are potential as antitumor and antibacterial agents. This study aims to obtain crude extracts from several types of starfish, and testing the antibacterial activity of crude starfish extracts against bacteria Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus. Test the antibacterial activity of crude starfish extracs Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata against bacteria E. coli, S. aureus, dan S. agalactiae use the agar diffusion method by means of a well.  L. l extract  can inhibit bacteria e. coli at an extract concentration of 1000 mg/ml with a diameter of inhibitory zone 11.0 mm, whereas at a concentration of 750 mg/ml the test bacteria can still grow on the bacteria S. agalactiae and S. aureus can still grow even though the extract has be given  L. l Keywords: Potential Starfish, Antibacterial Activity, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae Bintang laut merupakan salah satu spesies dari kelas Asteroidea, dan dikelompokkan kedalam filum Echinodermata. Filum Echinodermata  terdiri atas kurang lebih 6000 spesies dan semuanya hidup di air laut. Bintang laut  merupakan salah satu sumber penghasil senyawa bioaktif. Senyawa aktif dari bintang laut telah diketahui memiliki aktivitas antioksidan, antibakteri, antiinflamasi, antifungi dan imunostimulator, ada juga bintang laut biru yang potensial sebagai antitumor dan agen antibakteri. Penelitian ini bertujuan untuk mendapatkan ekstrak kasar dari beberapa jenis bintang laut, dan menguji aktivitas antibakteri ekstrak kasar bintang laut terhadap bakteri Streptococcus agalactiae, Escherichia coli dan  Staphylococcus aureus. Uji aktivitas antibakteri dari ekstrak kasar bintang laut Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata terhadap bakteri E. coli, S. aureus, dan S. agalactiae mengggunakan metode difusi agar dengan cara sumur. Ekstrak L. l mampu menghambat bakteri E.coli pada konsentrasi ekstrak 1.000 mg/ml dengan diameter zona hambat 11.0 mm, sedangkan pada konsentrasi 750 mg/ml bakteri uji masih bisa bertumbuh pada bakteri S. agalactiae dan S. aureus masih dapat bertumbuh walaupun telah diberikan ekstrak L. l.Kata Kunci : Potensi Bintang Laut, Aktivitas Antibakteri, Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus

scholarly journals ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS OF Solanum incanum AGAINST Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 4 (2) ◽  
pp. e183
Author(s):  
David Mutisya Musyimi ◽  
Tracy Ann Ashioya ◽  
George Opande ◽  
William Omuketi Emitaro
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Solanum Incanum ◽  
Zones Of Inhibition ◽  
Ethanol Extracts ◽  
And Staphylococcus Aureus

Human pathogenic microorganisms have developed resistance in response to indiscriminative use of commercial drugs. Plants produce many secondary metabolites with microbiocidal activity hence their use in traditional medicine. Herbalists in Kenya use medicinal plants including Solanum incanum in treating microbial infections. Though S. incanum has been used to treat different diseases in humans and animals, there is little information on antimicrobial activities of its extracts against Escherichia coli and Staphylococcus aureus. In this study, phytochemical analysis and antibacterial activity of solanum incanum leaves, roots and seeds extracts were determined. Ethanolic and aqueous extracts of leaf, root and seed of concentrations 25, 50, 75 and 100, and amoxicillin 25 mg/ml (control) with three replications were used for antibacterial analysis by the agar-well diffusion method. The results were subjected to analysis of variance at P < 0.05. Phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, steroids and tannins. Solanum incanum exhibited significant antibacterial effect against the two test bacteria. Ethanol extracts were more active than extracts against the bacteria. Ethanol extracts at 100% inhibited growth of Staphylococcus aureus more than the Escherichia coli. The zones of inhibition for Staphylococcus aureus were 35.0±0.6 mm, 30.94±0.3 mm and 30.14±0.64mm for seed, root and leaves respectively.On the other hand, the zones of inhibition for Escherichia coliat 100% ethanol were 27.20±0.06, 23.14±0.12 and 21.0±0.4 seed, root and leaves respectively.The results validate the use of these plants in ethnomedicine and potential of this plant in treating infections caused by the two bacteria.

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