Evaluation of antioxidant enzymes activity in canola under salt stress

2014 ◽  
Vol 2 (2) ◽  
pp. 88-92 ◽  
Author(s):  
Jafar Abili ◽  
Sajjad Zare

Salinity is one of the major stresses in arid and semi-arid regions causing adverse effects at physiological, biochemical, and molecular levels, limiting crop productivity. In this research, three canola cultivars (Licord, Talayeh, Zarfam) were compared at 5 salinity levels (control, 50, 100, 150 and 200 mM) for their catalase, guaiacol peroxidase, superoxide dismutase activity,proline and yield in a completely randomized design with 3 replications. In our study, we found that NaCl concentrations greater than 150 and 200 mM caused the irreversible disorders. Increased salt concentrations led to significant changes in the levels of antioxidative enzymes and proline in three canola cultivars. Also, yield rates in three varieties decreased in the presence of NaCl concentrations.

2016 ◽  
Vol 7 (1) ◽  
pp. 86 ◽  
Author(s):  
João Batista Dos Santos ◽  
Hans Raj Gheyi ◽  
Geovani Soares De Lima ◽  
Diego Azevedo Xavier ◽  
Lourival Ferreira Cavalcante ◽  
...  

The high salt concentrations found in irrigation formation of water in semi-arid regions lead to changes on crops growth and development and can lead to halomorphicsoils formation. In this context, it was proposed to evaluate the morphophysiology and production of colored herbaceous cotton BRS Topaz fertilized with nitrogen under a saline-sodic soil and submitted to the irrigation with water of different salinities. The experiment was conducted in an eutrophic sandy quartzarênico Neossol in the municipality of Campina Grande, Paraíba, Brazil, adopting a completely randomized design in 5x5 factorial with three repetitions, with five salinity levels of the the irrigation water (0 7; 2.7; 4.7; 6.7 and 8.7 dS m-1 at 25°C) and five levels of nitrogen (40; 60; 80; 100 and 120 mg of N kg-1). Regarding cotton growth, no effect was observed for the interaction between salinity and soil nitrogen doses. The absolute growth rate for height, stem diameter and leaf area decreased with an increase in the irrigation water electrical conductivity, being leaf area the most affected variable.The number of bolls and the cotton seed production per plant decreased with the salinity increase and increased with in increase of nitrogen fertilization.


FLORESTA ◽  
2019 ◽  
Vol 49 (4) ◽  
pp. 725
Author(s):  
Jorge Luís Fabricio Queiroz ◽  
Miguel Ferreira Neto ◽  
Francisco Vanies da Silva Sá ◽  
Francisco De Queiroz Porto Filho ◽  
Yuri Bezerra de Lima ◽  
...  

Species of the genus Spondias, such as ambarella, are propagated primarily by vegetative parts. However, for planting it is necessary to use plant regulators. In addition to propagation related aspects, the availability of good quality water for seedling production in semi-arid regions is a limiting factor. The objective of this work was to evaluate the rooting of semi-herbaceous cuttings of ambarella using different concentrations of IBA conveyed in solution and talc, irrigated with different concentrations of treated domestic effluent. The experiment was carried out in a nursery with 50% shade in a completely randomized design in a 4 x 4 factorial scheme consisting of four doses of treated domestic effluent (EDT) diluted in water supply [E1 = 0% of EDT (100% AA - control); E2 = 33.3% of EDT (66.7% of AA); E3 = 66.7% of EDT (33.3% of AA) and E4 = 100% of EDT (0% AA)] used in daily irrigation and four IBA dilution managements (M1 = control without IBA, M2 = IBA carried in water at 6,000 mg L-1, M3 = IBA delivered in alcohol (70%) at 4,000 mg L-1 and M4 = IBA in Talc at 5000 mg L-1), with four replicates. The treated domestic effluent is viable for irrigation of ambarella seedlings in its diluted or concentrated. The use of 5,000 mg L-1 of IBA delivered to talc is management satisfactory for the propagation of seedlings of ambarella by means of semi-herbaceous cuttings.


2021 ◽  
Vol 38 (2) ◽  
pp. 75-88
Author(s):  
Mairton Gomes Da Silva ◽  
Ancélio Ricardo De Oliveira Gondim ◽  
Eder Ramon Feitoza Ledo ◽  
Anna Hozana Francilino ◽  
Yasmin Da Silva ◽  
...  

Salinity is one of the striking problems in agricultural production in many parts of the world. Seed germination and seedling growth are two critical stages for the establishment of crops generally most sensitive to salt stress. The present study aimed at evaluating the germination and initial growth of pepper seedlings produced from seeds under different soaking times in NaCl solutions. The experiment was carried out in a completely randomized design, in a 2 × 4 × 5 factorial scheme using two pepper species (Capsicum chinense Jacq. and Capsicum frutescens L.), four levels of electrical conductivity (EC) of solutions (1.5, 3.0, 4.5, and 6.0 dS m-1) and five times of seed soaking in the solutions (2, 4, 6, 8 and 10 h), with three replications. The traits evaluated were the number of germinated seedlings, percentage of germination, seedling height, and root length. The results showed that C. frutescens pepper was more tolerant to different times of soaking in saline solutions prepared with NaCl compared to C. chinense. Thus, the results suggest that depending on the pepper species, it is recommended to use seeds primed in saline solutions with salinity levels compatible with those under field conditions (in saline soils and/or irrigation with saline waters).


Author(s):  
O. I. Horielova ◽  
◽  
N. I. Ryabchun ◽  
M. A. Shkliarevskyi ◽  
A. M. Reznik ◽  
...  

Along with specific adaptive reactions, universal defense reactions, in particular activation of antioxidant system, are of great importance for plant survival under cold conditions. We have studied a relationship among the content of low-molecular-weight protective compounds with antioxidant properties (proline, soluble carbohydrates, flavonoids), the activity of antioxidant enzymes (superoxide dismutase, catalase, and guaiacol peroxidase) in seedlings of winter wheat, rye and triticale, and frost resistance of etiolated seedlings and adult plants at tillering stage. It was found that there was a fairly close correlation between the frost resistance of seedlings and adult cereal plants (r = 0,78). It was shown that a pronounced relationship between individual indicators of antioxidant system functioning in unhardened seedlings and their frost resistance was not found. After 6-day hardening of seedlings at 2-4°C, there was a high correlation between the total indicator of the enzymatic antioxidant system (the sum of normalized indicators of superoxide dismutase, peroxidase, and catalase activity) and their frost resistance (r = 0,86), but the correlation coefficient of this index with frost resistance of plants in tillering phase was significantly lower (r = 0,47). At the same time, a high correlation was found between the content of low-molecular-weight protectors in hardened seedlings and frost resistance of tillering adult plants (r = 0.89). The closest correlation was observed between the integral normalized indicator, comprising the sum of normalized values of antioxidant enzymes activity and the content of low-molecular-weight protectors in hardened seedlings, and frost resistance of seedlings (r = 0,94) and plants in tillering phase (r = 0,89). A presence of specific features in the functioning of antioxidant system during cold adaptation of cereal seedlings was established. Rye is characterized by a high content of low-molecular-weight protective compounds; at the same time, increased activity of antioxidant enzymes - superoxide dismutase and catalase - was noted in wheat seedlings. In triticale, depending on the genotype, the values of both enzymatic antioxidant activity and the content of low-molecular-weight protectors varied.


2017 ◽  
Vol 30 (4) ◽  
pp. 1001-1008
Author(s):  
JOÃO PEDRO ALVES DE AQUINO ◽  
ANTÔNIO AÉCIO DE CARVALHO BEZERRA ◽  
FRANCISCO DE ALCÂNTARA NETO ◽  
CARLOS JOSÉ GONCALVES DE SOUZA LIMA ◽  
RAYLSON RODRIGUES DE SOUSA

ABSTRACT Cowpea is broadly cultivated worldwide, especially in semi-arid or arid regions where soil or irrigation water salt contents can negatively influence the species’ productive capacity. The objective of this study was to evaluate the morphophysiological responses of cowpea genotypes to irrigation water salinity. The experiment was conducted in a greenhouse, under a completely randomized design with nine replications and in a 5x3 factorial scheme. Treatments consisted of five levels of irrigation water electrical conductivity - EC (EC0: 0.55; EC1: 1.60; EC2: 3.20; EC3: 4.80 and EC4: 6.40 dS m-1), applied from the 15th day after sowing (DAS), and three cowpea genotypes (G1: BRS Imponente; G2: MNC04-795F-168 and G3: MNC04-795F-159). EC increases at 35 DAS promoted stem diameter reductions of 8.0% (G1), 11.4% (G2), and 7.7% (G3), indicating different resistance to salinity by each genotype. Leaf area reductions at 25 and 38 DAS were 30.9% and 38.8% for EC0 and EC4, respectively. The BRS Imponente cultivar presented a performance superior to those of G2 and G3 in relation to stem diameter and stem dry matter at 25 DAS, and root-shoot and root-leaf ratios at 38 DAS.


Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 256 ◽  
Author(s):  
Patrick Nyambo ◽  
Thembalethu Taeni ◽  
Cornelius Chiduza ◽  
Tesfay Araya

Soil acidification is a serious challenge and a major cause of declining soil and crop productivity in the Eastern parts of South Africa (SA). An incubation experiment investigated effects of different maize residue biochar rates on selected soil properties and soil loss in acidic Hutton soils. Biochar amendment rates were 0%, 2.5%, 5%, 7.5%, and 10% (soil weight) laid as a completely randomized design. Soil sampling was done on a 20-day interval for 140 days to give a 5 × 7 factorial experiment. Rainfall simulation was conducted at 60, 100 and 140 days after incubation to quantify soil loss. Relative to the control biochar amendments significantly improved soil physicochemical properties. After 140 days, biochar increased soil pH by between 0.34 to 1.51 points, soil organic carbon (SOC) by 2.2% to 2.34%, and microbial activity (MBC) by 496 to 1615 mg kg−1 compared to control. Soil aggregation (MWD) changes varied from 0.58 mm to 0.70 mm for the duration of the trial. Soil loss significantly decreased by 27% to 70% under biochar amendment compared to control. This indicates that maize residue biochar application has the potential to improve the soil properties and reduce soil loss in the degraded acidic Hutton soil.


2020 ◽  
Vol 44 ◽  
Author(s):  
Carlos Donato da Silva Souza ◽  
Geronimo Ferreira da Silva ◽  
Sirleide Maria de Menezes ◽  
José Edson Florentino de Morais ◽  
José Amilton Santos Júnior ◽  
...  

ABSTRACT Cultivation using brackish waters can result in nutritional and metabolic imbalances in several plant species, consequently reducing the production of dry matter (DM) and accumulation of toxic ions (Na+ and/or Cl-) in plants. We evaluated the DM production, and nutrient and inorganic solute (Na+ and Cl-) content in green onion plants (cv. Todo Ano Evergreen - Nebuka) under different levels of nutrient solution salinity in combination with circulation frequencies of this solution. Two experiments were conducted in a hydroponic system, using a completely randomized design, in a 6 × 2 factorial scheme, with five replicates: six levels of nutrient solution salinity (1.5, 3.0, 4.5, 6.0, 7.5, and 9.0 dS m-1) and two solution circulation frequencies (twice and thrice a day). In Experiment I, the evapotranspired depth was replaced using brackish water that was used to prepare each of the salinity levels (used exclusively), whereas in Experiment II, brackish water was used only to prepare each of the salinity levels and public water was used (electrical conductivity [ECw] = 0.12 dS m-1) for replacement in all treatments. The increase in the nutrient solution salinity reduced the production of DM and accumulation of nutrients; the reductions were more pronounced when brackish waters were used exclusively (Experiment I). However, the circulation of solutions thrice a day resulted in the harmful effects of the salinity effect. Replacing the evapotranspirated blade with water supply (Experiment II) mitigated the deleterious effects of salinity. Moreover, three circulations of the nutrient solution daily resulted in lower accumulation of inorganic Na+ and Cl- solutes and increased accumulation of nutrients N, P, K+, Ca2+, Mg2+, and S in the culture.


2018 ◽  
Vol 7 (4) ◽  
pp. 2139
Author(s):  
Savita J. ◽  
Somveer Jakhar

Salinity is one of the limiting environmental factors for crop production. Chickpea has special importance among the legumes especially in arid and semi-arid regions and is sensitive to salinity. Therefore, it becomes necessary to make a plan to mitigate the salinity effect on this plant. For this purpose, an experiment was conducted in the net house of Department of Botany, Kurukshetra University, Kurukshetra to investigate the role of sulfosalicylic acid (SSA) at different concentrations (10-4, 10-5 and 10-6 M) in overcoming salinity stress imposed on chickpea plants in natural conditions. Different salinity levels (0, 50 mM, 100 mM and 150 mM) were applied and caused a significant reduction in morphological and yield parameters. Our main findings are as follows: (1) Salt stress has detrimental effects on growth and physiology of plants. (2) Application of SSA at 10-5 M was the most significant concentration in modulating the inhibitory effects of salt stress.


2018 ◽  
Vol 10 (7) ◽  
pp. 455
Author(s):  
Hozano De Souza Lemos Neto ◽  
Marcelo De Almeida Guimarães ◽  
Rosilene Oliveira Mesquita ◽  
Italo Marlone Gomes Sampaio ◽  
Ana Régia Alves de Araújo Hendges ◽  
...  

Silicon (Si) is a mineral element that provides many benefits to plants and may also assist in tolerance to abiotic stresses such as salinity. The aim of the study was to evaluate the use of Si as a possible attenuator of the deleterious effects of salinity on growth and post-harvest of lettuce. A completely randomized design with four replicates was performed in a 3 × 2 factorial, consisting of three salinity levels (1.65, 3.65, 7.65 dS m-1) and two levels of silicon (0.0 and 2.0 mM). Salinity reduced all variables related to plant growth. For shoot fresh and dry mass, it was observed that NaCl caused a reduction of 79.80 and 80%, respectively, in comparison to the control. However, parameters related to post-harvest such as titratable acidity, soluble solids and vitamin C increased with salinity. Although not effective in reducing saline stress on plant growth, Si provided improvements in the variables related to lettuce post-harvest. Salinity significantly reduced plant growth in the presence and absence of Si, but provided better post-harvest quality with Si.


Author(s):  
Álvaro Renan Vieira Nunes ◽  
Eder Ferreira Arriel ◽  
Marcelo Soares Pimentel ◽  
Maria José de Holanda Leite ◽  
Samara Paulo dos Santos Fernandes ◽  
...  

Azadirachta indica A. Juss. of the Meliácea family originates from India. It is considered important in Brazil due to its multiple uses. The plant is usually propagated by sexual reproduction (seeds), however, after harvest the seeds have to be sown as soon as possible because they lose germination viability very quickly. The use of juvenile propagules for A. indica seedlings propagation is a viable option, since there is a high demand in the semi arid regions due to wide use in urban afforestation. The objective of this research was to evaluate the effect of fertilization regimens and environments on obtaining Apical cuttings of juvenile origin and on the quality of cloned seedlings of Azadirachta indica. The research was carried out at the Forest Nursery of UFCG/Patos-PB, Brazil, with vegetative propagules (cuttings) obtained from three environments and two fertilization regimes: Biweekly and monthly, in addition to the control (without fertilization). The experiment was arranged in completely randomized design, factorial 3 x 3 (environments of origin of cuttings x fertilization regimes), with six replications, where each plot consisted of a cutting, totaling 54 experimental plots. Results indicated that propagation of Azadirachta indica through cuttings of juvenile origin is feasible, with an average rooting of 95.2%. It is recommended to use the monthly fertilization regimen, since in general it did not differ significantly from the biweekly regime. Fertilization provided better development and quality of the root system and aerial parts.


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