Assessment of free radical scavenging activity and polyphenolic content in leaves of Neurocalyx calycinus (R. Br. ex Benn.) Rob. - a medicinal plant used by Cholanaickan tribe

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Ajayakumar Lalitha Aneeshkumar
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Dried Extract

Neurocalyx calycinus (R. Br. ex Benn.) Rob. is an endemic species of the family Rubiaceae, found in Western Ghats of India and Sri Lanka. The leaf paste of N. calycinus has been used by Cholanaickan tribe as medicine to heal wounds, burns, inflammation and pain. Many of the human diseases are manifested due to the accumulation of free radicals. Plant derived antioxidants have the power to scavenge these free radicals without any internal complications. Aqueous, n-hexane, chloroform, ethyl acetate and ethanol extracts of the leaves of N. calycinus were selected for qualitative phytochemical screening. DPPH, nitric oxide, superoxide and hydroxyl radical scavenging assays were carried out. Total phenolic, flavonoid and antioxidant content of all the extract was measured. Phytochemical screening showed that alkaloids, steroids, phenolic compounds, glycosides and saponins were found in the ethanolic extract. Ethanol extract showed better antioxidant activity than the other solvent extracts. The reducing power of all the extracts was increased dose dependently. Highest amount of phenolic (56.27 mg of gallic acid equivalent /gm of dried extract), flavonoid (36.92 mg of quercetin equivalent/gm of dried extract) and antioxidant (85.68 mg of ascorbic acid equivalent/gm of dried extract) content was found in the ethanolic extract. A positive correlation (R2) was found between the total phenolic content and free radical scavenging activity of the leaf extract of N. calycinus. The results confirmed that the phenolic compounds in the ethanolic extract of the leaves of N. calycinus are potent source of natural antioxidants.

scholarly journals Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 70 ◽  
Author(s):  
Kusum Sai ◽  
Rashmi Thapa ◽  
Hari Prasad Devkota ◽  
Khem Raj Joshi
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Inhibitory Activities

Background: More than 700 plants are reported to be used for medicinal purposes in Nepal; however, many of them are not studied for their scientific evidences. The aims of the present study were the estimation of the total phenolic and flavonoid contents, and the evaluation of the free radical scavenging and α-amylase inhibitory activities of five selected medicinal plants from western Nepal: Aeschynanthus parviflorus Wall. (Gesneriaceae), Buddleja asiatica Lour. (Loganiaceae), Carica papaya L. (Caricaceae), Drepanostachyum falcatum (Nees) Keng f. (Gramineae) and Spondias pinnata (L. f.) Kurz (Anacardiaceae). Methods: The total phenolic content (TPC) and total flavonoid content (TFC) were measured using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. A 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the free radical scavenging activity and an α-amylase inhibitory assay was carried out to determine the in vitro antidiabetic activity. Results: The phytochemical screening of five hydroalcoholic plant extracts revealed the presence of various secondary metabolites, including alkaloids, flavonoids, reducing sugars, saponins, terpenoids and tannins. The amounts of total phenolics and flavonoids were found to be the highest in B. asiatica leaf extract, which also showed the most potent free radical scavenging activity. Extract of C. papaya fruits showed the highest α-amylase inhibitory activity, whereas the extracts of B. asiatica leaves and S. pinnata leaves exhibited moderate activity. Conclusions: Some of the medicinal plants selected in this study showed high TPC and TFC values and potent bioactivities. These results may provide the scientific evidences of the traditional uses of these plants. However, further detailed studies on bioactive compounds isolation and identification and evaluation of in vivo pharmacological activities should be performed in future.

scholarly journals Evaluation of total phenolic content, free radical scavenging activity and phytochemical screening of different extracts of Averrhoa bilimbi (fruits)

2013 ◽  
Vol 2 (4) ◽  
pp. 92-96 ◽  
Author(s):  
Md. Hasanuzzaman ◽  
Md. Ramjan Ali ◽  
Marjan Hossain ◽  
Sourov Kuri ◽  
Mohammad Safiqul Islam
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening

The present study was designed to investigate the phytochemical screening, the free radical scavenging activity and to determine the total phenolic content of methanolic extract and different solvent soluble fractions of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits. The free radical scavenging activity was evaluated by analyzing the bleaching rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total phenolic content was determined by using Folin-Ciocalteau reagent, which results were expressed in gallic acid equivalent (mg of GAE/g of sample). The phytochemical screening revealed the potent source of different phytochemical constituents on different extractives including, phenol, flavonoid, tannin that are responsible for antioxidant action. In the determination of total phenolic content, different extractives showed a significant content of phenolic compounds ranging from 50.23-68.67 mg of GAE/g of extractive. The plant sample displayed significant DPPH free radical scavenging activity with highest IC50 value in crude methanolic extract (30.365 ?g/ml) followed by chloroform, carbon tetrachloride, pet-ether and aqueous soluble fractions having value of 32.852 ?g/ml, 36.708 ?g/ml, 50.35 ?g/ml, and 79.918 ?g/ml, respectively as opposed to that of the scavenging effects of BHT of 19.656 ?g/ml.DOI: http://dx.doi.org/10.3329/icpj.v2i4.14058 International Current Pharmaceutical Journal, March 2013, 2(4): 92-96

scholarly journals Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

2020 ◽  
Vol 10 (3) ◽  
pp. 133-136
Author(s):  
Ahlem Karbab ◽  
Kamel Mokhnache ◽  
Lekhmici Arrar ◽  
Abderahmane Baghiani ◽  
Seddik Khennouf ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Potential Health

The purpose of this study was to prepare three sub-fractions from Pituranthos scoparius roots (PSR), characterize their phytochemicals contents and to investigate their free radical scavenging activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl scavenging activities. Tannins, flavonoids, steroids, and other bioactive compounds were found in the different sub-fractions. The Ethyle acetate extract (EAE) and chloroform extract (ChE) exhibited the highest antioxidant activity using ABTS (17.8 ± 0.87 µg/mL and 18.15 ± 0.68 µg/mL), respectively. Whereas, Crude extract (CrE) have been presented strong hydroxyl scavenging activity (14.9 ± 0.8 µg/mL). This study indicates that PSR extracts has potent free radical scavenging, and may prove to be of potential health benefit as well as additional resources for natural antioxidants. Keywords: Medicinal plant; phytochemical screening; sub-fractions, free radical scavenging.

scholarly journals Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>

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