scholarly journals Anthelmintic efficacy and safety of selected medicinal plants against mixed gastrointestinal nematodes in artificially infected sheep

2018 ◽  
Vol 7 (4) ◽  
pp. 360-365
Author(s):  
John Kaunga Muthee ◽  
Keyword(s):  
Gastrointestinal Nematodes ◽  
Infected Sheep ◽  
Multi Drug Resistance ◽  
Efficacy And Safety ◽  
Worm Count ◽  
Laboratory Conditions ◽  
Hematological And Biochemical Parameters ◽  
And Control ◽  
Myrsine Africana ◽  
Rapanea Melanophloeos

There has been widespread resistance to anthelmintics by pathogenic helminths to an extent where there is multi-drug resistance against all major classes of conventional anthelmintics. This world-wide phenomenon calls for urgent search for different approaches to the control of helminthosis including novel anthelmintic products. The purpose of the study was to determine the efficacy and safety of selected plants, which are frequently used in the treatment and control of helminthosis, in artificially infected sheep under controlled laboratory conditions. The selected plant species were, Albizia anthelmintica Brongn, Embelia schimperi L., Myrsine africana L. and Rapanea melanophloeos (L.) Mez. Thirty six male Dorper lambs, aged between 6 and 8 months, artificially infected with mixed gastrointestinal nematodes (GIN) under controlled laboratory conditions, were used for the study. Efficacy was determined using percentage fecal egg count reduction test (FECRT %) and percentage total worm count reduction (TWCR %). Safety of the remedies was assessed using health, hematological and biochemical parameters. The FECR % against the mixed gastrointestinal nematodes was -55, 7.6, 34.2, 69.3 and 83.3% for Albizia anthelmintica, Embelia schimperi, Rapanea melanophloeos, albendazole and Myrsine africana respectively. TWCR% of 60.7, 44.6, 66, 69.7 and 35.6 percent were recorded for Albizia anthelmintica, Embelia schimperi, Myrsine Africana, Rapanea melanophloeos, and albendazole groups respectively. It was concluded that some of the remedies like M. africana have good efficacy at safe levels and should further be evaluated to determine the most optimum dosages. The gastrointestinal nematodes used in this study were resistant to albendazole

scholarly journals Anthelmintic efficacy of selected medicinal plants against gastrointestinal nematodes in naturally infected sheep in Kenya

2018 ◽  
Vol 7 (2) ◽  
pp. 111-115
Author(s):  
JK Muthee ◽  
Keyword(s):  
Anthelmintic Resistance ◽  
Gastrointestinal Nematodes ◽  
Herbal Remedies ◽  
Multi Drug Resistance ◽  
Traditional Health ◽  
Health Practitioners ◽  
Faecal Egg Count ◽  
Traditional Practitioners ◽  
And Control ◽  
Myrsine Africana

The use of plants, or their extracts, for the treatment of human and animal ailments, including helminthosis is steeped in antiquity. Anthelmintic resistance in pathogenic helminths has been spreading in prevalence and severity to a point where there is multi-drug resistance against the three major classes of anthelmintics. This globe-wide phenomenon calls for urgent search for different approaches to the control of helminthosis including novel anthelmintic products. The objective of this study was to evaluate the efficacy of plants, which are commonly used in the treatment and control of helminthosis, under the natural grazing conditions in Loitoktok Sub-County of Kenya. The plant species (Albizia anthelmintica, Myrsine africana, and Embelia schimperi were selected based on their availability and ethnopharmacological uses, as anthelmintics, by the traditional health practitioners in the area. Fifty sheep of mixed breeds, belonging to local herders, and naturally infected with mixed gastrointestinal nematodes were recruited for the study. The herbal remedies were prepared and administered by methods prescribed by the traditional practitioners. Their efficacy was determined using faecal egg count reduction test (FECRT). The percentage faecal egg count reduction against the mixed gastrointestinal nematodes was 59, -11, -31 and 87 for Myrsine africana, A. anthelmintica, E. schimperi and albendazole respectively. It was concluded that some of the remedies have some efficacy and further studies are needed to evaluate their potential as anthelmintics. There was also suspicion of development of anthemintic resistance to albendazole and herders needed to be advised accordingly on the proper use of conventional anthelmintic products

scholarly journals Phytochemical screening and cytotoxicity of selected plants used as anthelmintics in Loitoktok Sub-County, Kenya

2016 ◽  
Vol 5 (1) ◽  
pp. 15-19
Author(s):  
J.K Muthee ◽  
◽  
D.W Gakuya ◽  
J.M Mbaria ◽  
C.M Mulei ◽  
...  
Keyword(s):  
Anthelmintic Resistance ◽  
Lethal Dose ◽  
Gastrointestinal Nematodes ◽  
New Drugs ◽  
Farm Animals ◽  
Multi Drug Resistance ◽  
Phytochemical Screening ◽  
Traditional Health ◽  
Organic Extracts ◽  
And Control

The practice of traditional medicine is as old as the human race, and plants are an important source of research and development of new drugs. Anthelmintic resistance in human and animal pathogenic helminthes has been spreading in prevalence and severity to a point where there is multi-drug resistance against the three major classes of anthelmintics. It has become a global phenomenon in gastrointestinal nematodes of farm animals, and hence the need for novel anthelmintic products. The objectives of this study were to carry out phytochemical screening and determine bioactivity of plants which are commonly used in the treatment and control of helminthosis in LoitoktokSub-Countyof Kenya. The plant species (Albiziaanthelmintica, Myrsineafricana, Embeliaschimperi and Rapaneamelanophloeos) were selected based on their ethnopharmacological uses, as anthelmintics, by the traditional health practitioners. Phytochemical were screened in aqueous and organic extracts using standard methods and cytotoxicity determined using the Brine shrimp lethality test. Phytochemical detected in the extracts were, anthraquinones, flavonoids, glycosides, saponins, steroids, tannins and triterpenoids. Organic extracts were generally more cytotoxic than the aqueous extracts with median lethal dose (LC50) of 11 to 581 µg/ml. and 149 to 1000 µg/ml respectively. It was concluded that some of the plants used as anthelmintic remedies in Loitoktok contain different types of phytochemical which could be responsible for their cytotoxicity and anthelmintic properties. Further studies may be necessary to assess their potential as anthelmintics for possible drug development.

Thromboembolic complications prevention in the obstetric practice

2017 ◽  
pp. 19-24
Author(s):  
O.V. Grishchenko ◽  
◽  
V.V. Bobrytska ◽  
Keyword(s):  
Pulmonary Embolism ◽  
Comparison Group ◽  
Main Group ◽  
Control Group ◽  
Efficacy And Safety ◽  
Thromboembolic Complications ◽  
Moderate Risk ◽  
Obstetric Practice ◽  
Thrombotic Complications ◽  
And Control

The objective: To evaluate the clinical efficacy and safety of Enoxaparin-Pharmex for the prevention of thrombotic complications (pulmonary embolism) in the postoperative period in patients with moderate risk of these complications. Patients and methods. The study included 50 women after a caesarean section had an average degree of risk of pulmonary embolism. Patients were divided into the main group (n=25) and control group (n=25) in accordance with the treatment: patients of the main group received postoperative Еnoxaparin- Pharmex, group comparisons enoxaparin sodium (brand foreign manufacturer’s). Patients in both groups received the drug at a dose of 20 mg for 5 days, 1 time per day subcutaneously. Results. The research data analysis showed identity results of hemostasiogram of patients in the main group and the comparison group, no side effects after treatment in both groups. Conclusion. The clinical studies suggest the drug Enoxaparin-Pharmex is effective, safe LMWH, which can be used to prevent troboembolic complications, including post-operative treatment in obstetric practice. Spectrum of Enoxaparin-Pharmex can be extended to the prevention and treatment of thromboembolic conditions of varying severity with appropriate doses of the drug. Key words: Enoxaparin-Pharmex, prevention of pulmonary embolism.

Efficacy of anthelmintic Eprimek against gastrointestinal nematodes in sheep

2020 ◽  
Vol 14 (4) ◽  
pp. 99-103
Author(s):  
V. I. Kolesnikov
Keyword(s):  
Live Weight ◽  
Gastrointestinal Nematodes ◽  
Production Environment ◽  
Control Groups ◽  
The North ◽  
Helminth Eggs ◽  
Nematode Eggs ◽  
Flotation Technique ◽  
And Control ◽  
Experimental Group

The purpose of the research is studying the efficacy of Eprimek (Eprinomectin) against gastrointestinal nematodes in sheep.Materials and methods. A commercial experiment to study the antiparasitic efficacy of Eprimek was carried out in June 2020 on 300 lambs of the North Caucasian breed in a private flock of Filimonovskaya Village, Izobilnensky District, the Stavropol Territory, which were divided into two groups. The experimental group of lambs (290 animals) was injected Eprimek subcutaneously at the earset at a dose of 1 ml/50 kg of live weight (10 mg of Eprinomectin in 1 ml), and 10 lambs were not treated; they were used as control. We collected feces from the lambs of the experimental and control groups before administration of the drugs and after 15 and 30 days. Fecal samples were examined by the flotation technique with a saturated solution of ammonium nitrate with counting nematode eggs in 1 g of feces. The results were processed statistically.Results and discussion. Eprimek showed a decrease in the number of excreted helminth eggs from 225.1±28.2 to 4.1±2.3 in production environment at a dose of 1 ml/50 kg of live weight, according to coprological studies on the 15th day after treatment in the experimental group of lambs. The efficacy was 98.2%, and 70% of the animals were free from the infection. The intensity of infection of the control lambs by gastrointestinal nematodes was 131–151 eggs per 1 g of feces at 100% prevalence.

scholarly journals Attraction of Astylus variegatus (Germ.) (Coleoptera: Melyridae) by volatile floral attractants

2007 ◽  
Vol 64 (3) ◽  
pp. 305-307 ◽  
Author(s):  
Maurício Ursi Ventura ◽  
Tania Pereira ◽  
Daiane Heloisa Nunes ◽  
Iara Cintra de Arruda
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Beans ◽  
Phaseolus Vulgaris L ◽  
Release Rates ◽  
Plastic Strip ◽  
Feeding Stimulant ◽  
Laboratory Conditions ◽  
Diabrotica Speciosa ◽  
And Control ◽  
Lagenaria Vulgaris

The beetle Astylus variegatus (Germ.) (Coleoptera: Melyridae) is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L.) fields. Traps originally designed to capture Diabrotica speciosa (Germ.) (Coleoptera: Chrysomelidae), consisted of plastic bottles (2 L) with 150 holes (5-mm diameter) yellow gold painted and containing inside a plastic strip (3.5 <FONT FACE=Symbol>´</FONT> 25 cm) with Lagenaria vulgaris (L.) powder (0.28% B cucurbitacin - feeding stimulant and arrestant for diabroticites) sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap), 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days) was approximately 32 mg day-1 per dispenser under laboratory conditions. 1,4-dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.

scholarly journals In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep

2018 ◽  
Vol 6 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Suelen Carolina Silva Soares ◽  
Gilmar Cotrin de Lima ◽  
Antonio Carlos Laurentiz ◽  
Aline Féboli ◽  
Luciano Alves dos Anjos ◽  
...  
Keyword(s):  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Infected Sheep ◽  
Grape Pomace

Voluntary intake of a medicated feed block by grazing sheep is increased by gastrointestinal nematode infection

2012 ◽  
Vol 52 (12) ◽  
pp. 1136
Author(s):  
F. J. Fishpool ◽  
L. P. Kahn ◽  
D. J. Tucker ◽  
J. V. Nolan ◽  
R. A. Leng
Keyword(s):  
Haemonchus Contortus ◽  
Gastrointestinal Nematode ◽  
Gastrointestinal Nematodes ◽  
Infected Sheep ◽  
Self Medication ◽  
Blood Samples ◽  
Voluntary Intake ◽  
Grazing Sheep ◽  
Long Acting ◽  
To Receive

The aim of this study was to determine the rate, variability and repeatability of intake by grazing sheep of a medicated feed block (MFB) containing fenbendazole and investigate if infection with gastrointestinal nematodes altered consumption patterns of the MFB in the same grazing mob. In Experiment 1, 30 Merino wethers were given access to an MFB for two separate 1-week periods, with blood sampling at Days 2, 4 and 6 of each period to determine MFB intake. In Experiment 2, the wethers were selected based on previous MFB intake and allocated to receive an oral dose of 10 000 Trichostrongylus colubriformis and 3000 Haemonchus contortus (anthelmintic susceptible) or a long acting anthelmintic. After 5 weeks, sheep were given access to an MFB (1.5 mg fenbendazole/g) and eight blood samples were taken over 2 weeks to determine intake. In Experiment 1, individual MFB intake in Week 1 and Week 2 was positively correlated (P = 0.002, R2 = 0.287). Mean individual MFB intake in Experiment 1 and Experiment 2 was positively correlated (P = 0.008, R2 = 0.047). In Experiment 2, more infected wethers (95%) consumed the MFB than did uninfected wethers (79%) (P < 0.001) and infected wethers ate significantly more MFB over the first 4 days (P = 0.041) of access. All infected sheep consumed sufficient MFB to receive a therapeutic dose and worm egg counts in infected sheep declined from 2165 epg to 120 epg in the first week of access to MFB. The decline in differences in MFB intake between infected and uninfected sheep corresponded to the decline in worm egg count, suggesting the existence of self-medication with parasitism accounting for intake differences.

scholarly journals Anthelminthic resistance of gastrointestinal nematodes in sheep to monepantel treatment in central region of Rio Grande do Sul, Brazil

2018 ◽  
Vol 38 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Fernanda Ramos ◽  
Luiza P. Portella ◽  
Fernando de S. Rodrigues ◽  
Caroline Z. Reginato ◽  
Alfredo S. Cezar ◽  
...  
Keyword(s):  
Rio Grande ◽  
Gastrointestinal Nematodes ◽  
Infected Sheep ◽  
The Other ◽  
Control Group ◽  
Fecal Culture ◽  
Rio Grande Do Sul ◽  
Anthelminthic Resistance ◽  
On Farm ◽  
Experimental Group

ABSTRACT: Given the numerous reports of anthelminthic resistance of sheep nematodes to different anthelmintic compounds, this study aimed to evaluate the resistance status of gastrointestinal nematodes from naturally infected sheep to monepantel in the state of Rio Grande do Sul. Four farms that present extensive raising system and absence of anthelmintic treatment for 60 days were selected for the study. Lambs that present counts of eggs per gram of feces (EPG) ≥200 (sensitivity of 50 EPG) one day (D-1) before the treatment were select for the study and randomly separated into two groups, a control group and an experimental group treated with monepantel. Feces were collected 9 days after the treatment (D+9) for EPG counts and fecal culture. The monepantel was 100% effective only on 2. The efficacy found on farm 1, 3, and 4 were 2.82%, 25.8%, and 78.4%, respectably. There were no viable larvae post-treatment at farm 2, but the genera Haemonchus, Trichostrongylus, Cooperia, and Strongyloides were resistant to it at the other farms. This study shows the presence of parasites resistant to the treatment with monepantel, pointing to the importance of monitoring its efficacy in sheep flocks of Rio Grande do Sul, Brazil.

scholarly journals Advances in the Treatment of Prolactinomas

2006 ◽  
Vol 27 (5) ◽  
pp. 485-534 ◽  
Author(s):  
Mary P. Gillam ◽  
Mark E. Molitch ◽  
Gaetano Lombardi ◽  
Annamaria Colao
Keyword(s):  
Medical Therapy ◽  
Critical Analysis ◽  
Dopamine Agonists ◽  
Multimodal Therapy ◽  
Efficacy And Safety ◽  
Treatment Resistant ◽  
Tumor Mass ◽  
Molecular Therapies ◽  
Novel Approaches ◽  
And Control

Prolactinomas account for approximately 40% of all pituitary adenomas and are an important cause of hypogonadism and infertility. The ultimate goal of therapy for prolactinomas is restoration or achievement of eugonadism through the normalization of hyperprolactinemia and control of tumor mass. Medical therapy with dopamine agonists is highly effective in the majority of cases and represents the mainstay of therapy. Recent data indicating successful withdrawal of these agents in a subset of patients challenge the previously held concept that medical therapy is a lifelong requirement. Complicated situations, such as those encountered in resistance to dopamine agonists, pregnancy, and giant or malignant prolactinomas, may require multimodal therapy involving surgery, radiotherapy, or both. Progress in elucidating the mechanisms underlying the pathogenesis of prolactinomas may enable future development of novel molecular therapies for treatment-resistant cases. This review provides a critical analysis of the efficacy and safety of the various modes of therapy available for the treatment of patients with prolactinomas with an emphasis on challenging situations, a discussion of the data regarding withdrawal of medical therapy, and a foreshadowing of novel approaches to therapy that may become available in the future.

scholarly journals The Current Status of Clinical Research Involving Microneedles: A Systematic Review

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1113
Author(s):  
Seung-Yeon Jeong ◽  
Jung-Hwan Park ◽  
Ye-Seul Lee ◽  
Youn-Sub Kim ◽  
Ji-Yeun Park ◽  
...  
Keyword(s):  
Clinical Effect ◽  
Osteoporosis Treatment ◽  
Current Status ◽  
Current Evidence ◽  
Controlled Trials ◽  
Efficacy And Safety ◽  
Inclusion Criteria ◽  
Control Groups ◽  
Randomized Controlled ◽  
And Control

In recent years, a number of clinical trials have been published on the efficacy and safety of drug delivery using microneedles (MNs). This review aims to systematically summarize and analyze the current evidence including the clinical effect and safety of MNs. Three electronic databases, including PubMed, were used to search the literature for randomized controlled trials (RCTs) and clinical controlled trials (CCTs) that evaluated the therapeutic efficacy of MNs from their inception to 28 June 2018. Data were extracted according to the characteristics of study subjects; disorder, types, and details of the intervention (MNs) and control groups; outcome measurements; effectiveness; and incidence of adverse events (AEs). Overall, 31 RCTs and seven CCTs met the inclusion criteria. Although MNs were commonly used in skin-related studies, evaluating the effects of MNs was difficult because many studies did not provide adequate comparison values between groups. For osteoporosis treatment, vaccine, and insulin delivery studies, MNs were comparable to or more effective than the gold standard. Regarding the safety of MNs, most AEs reported in each study were minor (grade 1 or 2). A well-designed RCT is necessary to clearly evaluate the effectiveness of MNs in the future.

Sign in / Sign up

Export Citation Format

Share Document