scholarly journals Changes in Biceps femoris Transcriptome along Growth in Iberian Pigs Fed Different Energy Sources and Comparative Analysis with Duroc Breed

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3505
Author(s):  
Rita Benítez ◽  
Yolanda Núñez ◽  
Miriam Ayuso ◽  
Beatriz Isabel ◽  
Miguel A. Fernández-Barroso ◽  
...  
Keyword(s):  
Muscle Development ◽  
Live Weight ◽  
Biceps Femoris ◽  
Energy Sources ◽  
High Oleic Sunflower Oil ◽  
Factors Affecting ◽  
Biceps Femoris Muscle ◽  
High Oleic ◽  
Cytoskeleton Organization ◽  
Genome Wide

This experiment was conducted to investigate the effects of developmental stage, breed, and diet energy source on the genome-wide expression, meat quality traits, and tissue composition of biceps femoris muscle in growing pure Iberian and Duroc pigs. The study comprised 59 Iberian (IB) and 19 Duroc (DU) animals, who started the treatment at an average live weight (LW) of 19.9 kg. The animals were kept under identical management conditions and fed two diets with different energy sources (6% high oleic sunflower oil or carbohydrates). Twenty-nine IB animals were slaughtered after seven days of treatment at an average LW of 24.1 kg, and 30 IB animals plus all the DU animals were slaughtered after 47 days at an average LW of 50.7 kg. The main factors affecting the muscle transcriptome were age, with 1832 differentially expressed genes (DEGs), and breed (1055 DEGs), while the effect of diet on the transcriptome was very small. The results indicated transcriptome changes along time in Iberian animals, being especially related to growth and tissue development, extracellular matrix (ECM) composition, and cytoskeleton organization, with DEGs affecting relevant functions and biological pathways, such as myogenesis. The breed also affected functions related to muscle development and cytoskeleton organization, as well as functions related to solute transport and lipid and carbohydrate metabolism. Taking into account the results of the two main comparisons (age and breed effects), we can postulate that the Iberian breed is more precocious than the Duroc breed, regarding myogenesis and muscle development, in the studied growing stage.

scholarly journals Breed, Diet, and Interaction Effects on Adipose Tissue Transcriptome in Iberian and Duroc Pigs Fed Different Energy Sources

Genes ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 589 ◽  
Author(s):  
Rita Benítez ◽  
Nares Trakooljul ◽  
Yolanda Núñez ◽  
Beatriz Isabel ◽  
Eduard Murani ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Immune Cell ◽  
Live Weight ◽  
Joint Analysis ◽  
Energy Sources ◽  
Cell Trafficking ◽  
Rna Seq ◽  
High Oleic Sunflower Oil ◽  
High Oleic ◽  
Subcutaneous Adipose

In this study, we analyzed the effects of breed, diet energy source, and their interaction on adipose tissue transcriptome in growing Iberian and Duroc pigs. The study comprised 29 Iberian and 19 Duroc males, which were kept under identical management conditions except the nutritional treatment. Two isoenergetic diets were used with 6% high oleic sunflower oil (HO) or carbohydrates (CH) as energy sources. All animals were slaughtered after 47 days of treatment at an average live weight of 51.2 kg. Twelve animals from each breed (six fed each diet) were employed for ham subcutaneous adipose tissue RNA-Seq analysis. The data analysis was performed using two different bioinformatic pipelines. We detected 837 and 1456 differentially expressed genes (DEGs) according to breed, depending on the pipeline. Due to the strong effect of breed on transcriptome, the effect of the diet was separately evaluated in the two breeds. We identified 207 and 57 DEGs depending on diet in Iberian and Duroc pigs, respectively. A joint analysis of both effects allowed the detection of some breed–diet interactions on transcriptome, which were inferred from RNA-Seq and quantitative PCR data. The functional analysis showed the enrichment of functions related to growth and tissue development, inflammatory response, immune cell trafficking, and carbohydrate and lipid metabolism, and allowed the identification of potential regulators. The results indicate different effects of diet on adipose tissue gene expression between breeds, affecting relevant biological pathways.

scholarly journals Genetic parameters for carcass traits and body size of meat sheep

2016 ◽  
Vol 37 (4Supl1) ◽  
pp. 2477
Author(s):  
Luciano Silva Sena ◽  
Gleyson Vieira dos Santos ◽  
Tatiana Saraiva Torres ◽  
Antônio De Sousa Júnior ◽  
Aurino De Araújo Rego Neto ◽  
...  
Keyword(s):  
Body Size ◽  
Variance Components ◽  
Live Weight ◽  
Biceps Femoris ◽  
Direct Selection ◽  
Biceps Femoris Muscle ◽  
Trait Analysis ◽  
Fat Thickness ◽  
Selection For ◽  
Estimate Variance

The present study aimed to estimate variance components and heritability coefficients (h2) in Santa Inês sheep by single- and two-trait analyses for the traits loin eye area (LEA), length (LEL) and maximum depth (LED), in the longissimus dorsi muscle, subcutaneous fat thickness (SFT), fat thickness over the biceps femoris muscle (FTBF), croup height (CH), thoracic circumference (TC), thoracic depth (TD), body length (BL), and adult live weight (ALW). The restricted maximum likelihood (REML) was used to estimate variance components in an animal model. In both types of analyses, we estimated moderate heritability for all traits, with the exception of SFT, CH, TC, BL, and ALW (all of which yielded low h2 estimates). In two-trait analysis for body size, only TD yielded a lower h2 estimate, when compared to single-trait analysis. On the other hand, CH, TC, and BL yielded higher h2 estimates. Most estimates for variance components and h2 in two-trait analysis were higher than those in single-trait analysis. This finding suggests the possibility of a moderate response to selection for improvement in the carcass of meat sheep using the specific carcass traits evaluated (except SFT), highlighting LEA. The fat thickness over the biceps femoris muscle showed higher potential for response to direct selection for fat deposition than SFT. Thoracic depth showed higher potential for response to direct selection for body size than the other morphometric measurements. In the present study, adult live weight was not an effective criterion for direct selection.

The prediction of voluntary intake of grazing dairy cows

1986 ◽  
Vol 107 (1) ◽  
pp. 43-54 ◽  
Author(s):  
Lindsey Caird ◽  
W. Holmes
Keyword(s):  
Organic Matter ◽  
Measurement Techniques ◽  
Live Weight ◽  
Simple Equation ◽  
Factors Affecting ◽  
Farm Planning ◽  
Sward Height ◽  
The Mean ◽  
Major Factors ◽  
Grazing Dairy Cows

SUMMARYInformation on the total organic matter intake, concentrates supplied (C), live weight (LW), week of lactation (WL), milk yield (MY), herbage organic matter digestibility (HOMD), herbage mass, sward height (SHT) or herbage allowance (HAL) measured individually for 357 cows at one of three sites was assembled. Observed intake was compared with intakes predicted by existing intake equations and new prediction equations based on regression models or regression and least-squares constants were developed. Major factors affecting intake were MY, LW, WL, C and HAL or SHT. Although HOMD was correlated with intake, better predictions were obtained when HOMD was omitted. There were differences between sites possibly associated with differences in measurement techniques.The predictive value of some existing equations and new equations were tested against independent sets of data. A simple equation (A) based on MY and LW (Ministry of Agriculture, Fisheries and Food, 1975) gave satisfactory average predictions but the mean square prediction error (MSPE) was high. The equations of Vadiveloo & Holmes (1979) adjusted for bias gave a relatively low MSPE. The preferred new equations for grazing cattle included MY, LW, WL, C and HAL or SHT, and their MSPE were similar to or lower than for indoor equations.The discussion indicates that a simple equation (A) would give adequate predictions for farm planning. The more detailed equations illustrate the inter-relations of animal with sward conditions and concentrate allowances. Predicted intakes may deviate from actual intakes because of short-term changes in body reserves.

Factors affecting voluntary feed intake in pigs. II. The effect of two levels of crude fibre in the diet on the intake and performance of fattening pigs

1967 ◽  
Vol 9 (2) ◽  
pp. 149-154 ◽  
Author(s):  
D. J. A. Cole ◽  
J. E. Duckworth ◽  
W. Holmes
Keyword(s):  
Feed Intake ◽  
Live Weight ◽  
Carcass Quality ◽  
Intake Rate ◽  
Crude Fibre ◽  
Factors Affecting ◽  
Voluntary Feed Intake ◽  
And Performance ◽  
Isocaloric Diets ◽  
Fattening Pigs

1. Voluntary feed intake, performance and carcass quality were studied with 20 individually housed pigs given isocaloric diets (2,770 kcal/kg. DM) containing either 8·9 % or 12·9 % crude fibre in the dry matter over the period of growth from 45 to 91 kg. live-weight.2. There were no significant differences between diets in voluntary feed intake, rate of gain or carcass quality.3. Apparent digestibility coefficients and rates of passage of the diets were determined in eight castrated male pigs. Rates of passage were not significantly affected by diet or live-weight when determined at 57 and 82 kg. live-weight.

scholarly journals Sequential alterations in the diameters of capillaries in rabbit skeletal muscle following deep transverse friction - a morphometric study

2005 ◽  
Vol 61 (2) ◽  
Author(s):  
M. A. Gregory ◽  
M. N. Deane ◽  
M. Marsh
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Muscle Blood Flow ◽  
Biceps Femoris ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Biceps Femoris Muscle ◽  
Beneficial Effects ◽  
The Cross

Objective: The precise mechanisms by which massage promotes repair in injured soft tissue are unknown. Various authorshave attributed the beneficial effects of massage to vasodilation and increased skin and muscle blood flow. The aim of this study was to determine whether deep transverse friction massage (DTF) causes capillary vasodilation in untraumatised skeletal muscle. Setting: Academic institution.Interventions: Twelve New Zealand white rabbits were anaesthetised and the left biceps femoris muscle received 10 minutes of DTF. Following treatment, wedge biopsies were taken from the musclewithin 10 minutes of treatment (R1 - 4), 24 hours (R5 - 8) and 6 days(R9 - 12) after treatment. To serve as controls, similar biopsies weretaken from the right biceps femoris of animals. The samples were fixed, dehydrated and embedded in epoxy resin.Transverse sections (1µm) of muscle were cut, stained with 1% aqueous alkaline toluidine blue and examined with a light microscope using a 40X objective. Images containing capillaries were captured using an image analyser with SIS software and the cross sectional diameters of at least 60 capillaries were measured from each specimen. Main Outcome Measures: Changes in capillary diameter. Results: The mean capillary diameters in control muscle averaged 4.76 µm. DTF caused a significant immediate increase of 17.3% in cross sectional area (p<0.001), which was not significantly increased by 10.0% after 24 hours (p>0.05). Six days after treatment the cross-sectional area of the treated muscle was 7.6% smaller than the controls. Conclusions: This confirms the contention that DTF stimulates muscle blood flow immediately after treatment and this may account for its beneficial effects in certain conditions. 

scholarly journals NEUROMUSCULAR CHARACTERISTIC OF BICEPS FEMORIS MUSCLE IN THE TOP SERBIAN SOCCER PLAYERS MEASURED BY TENSIOMYOGRAPHY METHOD: QUANTITATIVE MODEL

2019 ◽  
pp. 167
Author(s):  
Miloslav Fabok ◽  
Bojan Leontijević ◽  
Lazar Tomić ◽  
Milivoj Dopsaj
Keyword(s):  
Body Height ◽  
Biceps Femoris ◽  
Football Player ◽  
Quantitative Model ◽  
The Body ◽  
Locomotor System ◽  
Biceps Femoris Muscle ◽  
Significant Difference ◽  
Transition Training ◽  
Femoris Muscle

The main aim of this study was to define the quantitative neuromuscular characteristics of Biceps Femoris muscle (BF) as the knee joint flexor, i.e. the major synergist of caudal body in all specific movement tasks of a football player, measured by tensiomyography (TMG) method . The secondary aim of the study was to compare all TMG-BF characteristics in relation to bilateral dimorphism, as well as to compare dominant and non-dominant legs. The research was conducted on a sample which included 54 professional players of age 23.0 ± 4.4 years; body height: 182.6 ± 15.1 cm; body mass: 81.2 ± 15.1 kg; BMI: 23.3 ± 1.2 kg/cm2. TMG variables were measured on the muscles during a transition training phase (mid-season and end of the season). The results have shown that the average Tc - 32.19 ± 7.64 and 33.21 ± 8.88 ms, Td - 25.56 ± 3.58 and 25.44 ± 3.20 ms, and Dm - 7.39 ± 1.87 and 7.52 ± 2.50 mm, for dominant and non-dominant leg, respectively. The results have indicated that there was no statistically significant difference between the examined TMG variables of dominant and non-dominant leg (Wilks' lambda Value = 0.979, F=0.300, p=0.952, Part. Ƞ2 = 0.021). It may be stated that there are no differences in the manifestation of neuromuscular characteristics in healthy elite football players regardless of their dominant leg. A high level of preparedness in football in addition to a completely healthy status of the body and locomotor system result in a complete neuromuscular contractile symmetry BF of both legs.

scholarly journals Some Environmental Factors Affecting Birth Weight, Weaning Weight and Daily Live Weight Gain of Holstein Calves

2015 ◽  
Vol 3 (7) ◽  
pp. 617 ◽  
Author(s):  
Erdal Yaylak ◽  
Hikmet Orhan ◽  
Alim Daşkaya
Keyword(s):  
Weight Gain ◽  
Birth Weight ◽  
Environmental Factors ◽  
Live Weight ◽  
Weaning Weight ◽  
Factors Affecting ◽  
Live Weight Gain ◽  
Weaning Age ◽  
Humidity Index ◽  
Holstein Calves

The present study was conducted to determine some environmental factors affecting birth weight, weaning weight and daily live weight gain of Holstein calves of a livestock facility in Izmir, Turkey. The data on 2091 calves born between the years 2005-2010 were used to assess the relevant parameters. Effects of calving year, calving month, calf gender and the interaction between calving year and calving month on calves’ birth weights were highly significant. The overall mean of birth weights was 39.6±0.15 kg. In addition, effects of calving year, calving month, gender, birth weight, weaning age, calving year x calving month, calving year x gender and calving year x calving month x gender interactions on weaning weight (WW) and daily live weight gain (DLWG) were highly significant. The overall means of WW and DLWG were respectively found to be 79.7±0.20 kg and 525±2.5 g. A one kilogram increase in birth weight resulted in an increase of 0.89 kg in weaning weight and a decrease of 1.26 g in daily live weight gain. Prenatal temperature-humidity index (THI) affected birth weight of calves (R2=0.67). Increasing THI from 50 to 80 resulted in 3.8 kg decrease in birth weight.

scholarly journals Comparison of physical and qualitative traits of meat of two Polish conservative flocks of ducks

2004 ◽  
Vol 47 (4) ◽  
pp. 367-375 ◽  
Author(s):  
T. Kisiel ◽  
J. M. Książkiewicz
Keyword(s):  
Subcutaneous Fat ◽  
Biceps Femoris ◽  
Point Of View ◽  
Muscle Fibres ◽  
Mean Values ◽  
Qualitative Traits ◽  
Biceps Femoris Muscle ◽  
Leg Muscles ◽  
L Value ◽  
Femoris Muscle

Abstract. In two Polish conservative flocks of Miniduck (K2) and Pekin (P33), registered by FAO as domestic genetic resources (World Watch List, 2000) the mean values of physical (tissue components) and qualitative traits of meat (pH15, pH24, colour of meat) were determined. The experimental flocks showed significant variations in body weight at 7 weeks of age (1540 vs 2088 in drakes and 1395 vs 1986 g in ducks), in the proportion of breast and leg muscles and skin with subcutaneous fat in eviscerated carcass. K2 ducks are characterized by outstanding musculature of breast and P33 ducks by that of lower leg and by low fatness. pH15 values of breast and leg muscles in K2 and P33 birds were greater than pH24 of these muscles. In both flocks pH15 values of 6.09 to 6.33 and pH24 values of 5.90 to 6.05 in breast muscles were lower than in leg muscles (6.33 to 6.46 and 6.12 to 6.37, respectively). A significantly lower L* value (darker colour) of breast muscle was characteristic of K2 birds (43.7 in males and 40.5 in females) compared to P33 males and females. Furthermore, breast muscles of K2 drakes and ducks had significantly higher redness (a*) and yellowness values (b*) than P33. In pectoralis superficialis muscle of K2 and P33 females, a significantly lower diameter was found for white muscle fibres (αW) (30.0 to 30.5 μm) and for red muscle fibres (βR) (16.9 to 17.6 μm) than in biceps femoris muscle (αW from 48.3 to 54.2; βR from 36.0 to 37.1 μm). Muscle fibres of K2 compared to P33 ducks were characterized by significantly greater diameters of βR muscle fibre in pectoralis superficialis muscle, and by lower αW and βR fibre diameters in biceps femoris muscle. The experiment showed that meat of ducks from the conservative flocks studied is valuable from the consumer’s point of view.

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