scholarly journals Anti-Inflammatory Effect on Colitis and Modulation of Microbiota by Fermented Plant Extract Supplementation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 55
Author(s):  
Manabu Sugimoto ◽  
Toshiro Watanabe ◽  
Motoko Takaoka ◽  
Kyoko Suzuki ◽  
Tadatoshi Murakami ◽  
...  
Keyword(s):  
Plant Extract ◽  
Fecal Microbiota ◽  
Fermented Food ◽  
Fermented Foods ◽  
Bloody Stool ◽  
Microbiota Composition ◽  
Inflammation Markers ◽  
Positive Group ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Although results of recent studies suggest that fermented foods strongly affect the gut microbiota composition and that they relieve inflammatory bowel disease symptoms, some reports have described that fermented foods increase some inflammation markers based on differences in fermented food materials. This study evaluated the effects of fermented plant extract (FPE) on dextran sulfate sodium (DSS)-induced colitis in mice and the effects on fecal microbiota composition in humans. Mice fed 5% FPE with 3% DSS (FPE group) showed no body weight loss, atrophy of colonic length, or bloody stool, similar to mice fed a basal diet (negative group), whereas mice fed 3% DSS (positive group) exhibited those effects. Concentrations of inflammation markers IL-6 and TNF-α were not significantly different between FPE and negative groups; however, those concentrations became higher in the positive group. 16S ribosomal RNA gene sequencing was used to characterize fecal microbiota in healthy women before and after 3-month FPE supplementation. The FPE supplementation induced increases in Firmicutes phyla and in Clostridiales order, which play a central role in inflammation suppression. These results suggest that FPE enhances Clostridiales growth in the gut and that it has an anti-inflammatory effect.

scholarly journals Anti-Inflammatory Activity of Extract Mixture of Annona senegalensis Pers. and Piliostigma thonningii (Schum.)

2020 ◽  
Vol 10 (6-s) ◽  
pp. 103-108
Author(s):  
Komlatsè Togbenou ◽  
Kokou Idoh ◽  
Kossivi Dosseh ◽  
Tchazou Kpatcha ◽  
Amégnona Agbonon
Keyword(s):  
Nitric Oxide ◽  
Ethanolic Extract ◽  
No Production ◽  
Leukocyte Infiltration ◽  
Inflammation Markers ◽  
Asthma Model ◽  
Piliostigma Thonningii ◽  
Anti Inflammatory ◽  
Inflammatory Component ◽  
Inflammatory Effect

Introduction: Annona senegalensis Pers (Annonaceae) and Piliostigma thonningii (Schum.) (Leguminoseae)  are two medicinal plants used, often in combination, in traditional Togolese medicine for the treatment of diseases with an inflammatory component. Objective: The aim of this study is to evaluate the anti-inflammatory effect of the hydro-ethanolic extract (EHEM) of the combination of A. senegalensis and P. thonningii (1: 1, m: m). Methods: The ovalbumin-induced allergic airway asthma model was used. Animals made asthmatic were treated with EHEM at doses of 250 and 500 mg / kg. Inflammation markers including histamine, nitric oxide (NO), vascular leakage, leukocyte infiltration in the airways, and malondialdehyde (MDA), were measured. Results: Compared to the SNT group, EHEM inhibits the infiltration of the airways by leukocytes (850,00 × 103 ± 50 cells / mL vs 1830 × 103 ± 53,85 cells / mL for ST500 mg / kg, P <0,05). It inhibited vascular permeability to Evans Blue (10,40 ± 0,270 μg / mL vs ST500, p<0,05). It inhibited histamine release (13,95 ± 0,937 μg / mL vs 32,78 ± 1,044 μg / mL, p<0,05) and NO production (0,211 ± 0,008 Μm vs 0,315 ± 0,022 μM, p<0,05). It finally inhibited MDA production (14,66 ± 0,533 nM / mL vs 9,014 ± 0,366 nM / mL and 7,149 ± 0,300 nM / mL, p<0,05) in lung tissue. Conclusion: Our results suggest that EHEM inhibits OVA-induced inflammation. These results justify the use of this combination of plants in traditional Togolese medicine. Keywords: Inflammation, malondialdehyde, Annona senegalensis, Piliostigma thonningii.

scholarly journals Leucas aspera to Treat Pan Oral Disorders - An Experimental Study.

2019 ◽  
Vol 1 (Issue 1) ◽  
pp. 26-33
Author(s):  
S. Karthika Nagarajan ◽  
Vidya Rani ◽  
Aravindhababu Narayanasamy ◽  
Mahaboob Kadar Masthan
Keyword(s):  
Experimental Study ◽  
Plant Extract ◽  
Phytochemical Analysis ◽  
Leucas Aspera ◽  
Raw 264.7 Macrophage Cells ◽  
Consistent Increase ◽  
Anti Inflammatory ◽  
High Level ◽  
Powder Extract ◽  
Inflammatory Effect

This article aimed at discussing the effectiveness of developing a new antioxidant and anti-inflammatory product from the natural source namely the extract of Leucas aspera plant that can be used to treat various oral mucosal conditions. The study was a descriptive experimental study. The dried powder extract of the whole LA plant was subjected to various procedures such as phytochemical analysis, antioxidant assay (DPPH) and assessment of anti-inflammatory effect (Cytokine activity in RAW 264.7 macrophage cells) and the results were tabulated. Significance of anti-inflammatory activity was tested by T-test. Our analysis has confirmed that 100µg of plant extract showed high amount of antioxidant activity (78%). The cytokine inhibitory percentage of our plant extract showed consistent increase when tested from 250 ug/ml to 1000ug/ml to be from 24% to 39% thus demonstrating significantly high level of anti-inflammatory potential (P > 0.01). Our study suggests that LA plant can be safely utilized for testing and developing products based on its extract.

scholarly journals Microbiota and Metabolite Modifications after Dietary Exclusion of Dairy Products and Reduced Consumption of Fermented Food in Young and Older Men

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1905
Author(s):  
Jinyoung Kim ◽  
Kathryn J. Burton-Pimentel ◽  
Charlotte Fleuti ◽  
Carola Blaser ◽  
Valentin Scherz ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dairy Products ◽  
Fermented Food ◽  
Fermented Foods ◽  
Microbiota Composition ◽  
Microbial Composition ◽  
Age Related ◽  
The Impact ◽  
Effect Of Age ◽  
Gut Microbiota Composition

The gut microbiota adapts to age-related changes in host physiology but is also affected by environmental stimuli, like diet. As a source of both pre- and probiotics, dairy and fermented foods modulate the gut microbiota composition, which makes them interesting food groups to use for the investigation of interactions between diet and ageing. Here we present the effects of excluding dairy products and limiting fermented food consumption for 19 days on gut microbiota composition and circulating metabolites of 28 healthy, young (YA) and older (OA) adult men. The intervention affected gut microbial composition in both groups, with significant increases in Akkermansia muciniphila and decreases in bacteria of the Clostridiales order. Lower fasting levels of glucose and insulin, as well as dairy-associated metabolites like lactose and pentadecanoic acid, were observed after the intervention, with no effect of age. The intervention also decreased HDL and LDL cholesterol levels. Dairy fat intake was positively associated with the HDL cholesterol changes but not with the LDL/HDL ratio. In conclusion, restricting the intake of dairy and fermented foods in men modified their gut microbiota and blood metabolites, while the impact of the dietary restrictions on these outcomes was more marked than the effect of age.

scholarly journals Leucas aspera to Treat Pan Oral Disorders - An Experimental Study

2019 ◽  
pp. 26-33
Author(s):  
S. Karthika Nagarajan ◽  
Vidya Rani ◽  
Aravindhababu Narayanasamy ◽  
Mahaboob Kadar Masthan
Keyword(s):  
Experimental Study ◽  
Plant Extract ◽  
Phytochemical Analysis ◽  
Leucas Aspera ◽  
Raw 264.7 Macrophage Cells ◽  
Consistent Increase ◽  
Anti Inflammatory ◽  
High Level ◽  
Powder Extract ◽  
Inflammatory Effect

This article aimed at discussing the effectiveness of developing a new antioxidant and anti-inflammatory product from the natural source namely the extract of Leucas aspera plant that can be used to treat various oral mucosal conditions. The study was a descriptive experimental study. The dried powder extract of the whole LA plant was subjected to various procedures such as phytochemical analysis, antioxidant assay (DPPH) and assessment of anti-inflammatory effect (Cytokine activity in RAW 264.7 macrophage cells) and the results were tabulated. Significance of anti-inflammatory activity was tested by T-test. Our analysis has confirmed that 100µg of plant extract showed high amount of antioxidant activity (78%). The cytokine inhibitory percentage of our plant extract showed consistent increase when tested from 250 ug/ml to 1000ug/ml to be from 24% to 39% thus demonstrating significantly high level of anti-inflammatory potential (P > 0.01). Our study suggests that LA plant can be safely utilized for testing and developing products based on its extract.

scholarly journals Comparative Study of Anti-Inflammatory Effect on DSS-Induced Ulcerative Colitis Between Novel Glycyrrhiza Variety and Official Compendia

2021 ◽  
Vol 11 (4) ◽  
pp. 1545
Author(s):  
Sa-Haeng Kang ◽  
Young-Jae Song ◽  
Yong-Deok Jeon ◽  
Dong-Keun Kim ◽  
Jeong-Hyang Park ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Activity Index ◽  
Clinical Signs ◽  
Body Weight Loss ◽  
Disease Activity Index ◽  
Fecal Microbiota ◽  
Specific Pattern ◽  
Plant Cultivation ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Glycyrrhizae radix (GR), a plant commonly referred to as licorice, is used as a medicine and food worldwide. However, the utilization of GR from wild areas has caused desertification and a depletion of natural resources. Environmental restrictions and low productivity have limited plant cultivation. For this reason, an improved Glycyrrhiza variety, Wongam (WG), in cultivation and quality has been developed by Korea Rural Development Administration. To evaluate the equivalence of efficacy, several comparative studies between already-registered species and new cultivars have been conducted. This study evaluated the anti-inflammatory effect of WG extracts in a dextran sulfate sodium (DSS)-induced colitis model, in comparison to that of GR extracts. WG extract significantly improved the clinical signs of DSS-induced ulcerative colitis, including disease activity index, body weight loss, and colon length shortening, which was equivalent to the effect of GR. Furthermore, the fecal microbiota was analyzed by terminal restriction fragment length polymorphism. The composition of the fecal microbiota did not show a specific pattern based on experimental groups; however, a tendency toward an increase in the proportion of Lactobacillales was observed. These findings showed an equivalence of efficacy and the possible utilization of WG as a medicinal resource with already-registered species.

scholarly journals Anti-inflammatory Effect of Lactobacillus Plantarum IDCC 3501 and Its Safety Evaluation

2021 ◽  
Author(s):  
Soo-Yeon Yang ◽  
Seung A Chae ◽  
Won Yeong Bang ◽  
O-Hyun Ban ◽  
Soo-Jung Kim ◽  
...  
Keyword(s):  
Lactate Production ◽  
Safety Evaluation ◽  
Fermented Food ◽  
Human Consumption ◽  
No Production ◽  
Raw 264.7 Macrophages ◽  
Minimal Inhibitory Concentrations ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract This study investigated the anti-inflammatory activity of L. plantarum IDCC 3501 isolated from kimchi (Korean fermented food) and its safety. When lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were treated with cell-free supernatant from L. plantarum IDCC 3501, the mRNA expression level of inflammatory markers (i.e., TNF-α, IL-1β, and IL-6) was significantly reduced. The decreased cell viability by LPS was recovered and NO production in LPS-induced cell was also decreased. The genes responsible for antibiotic resistance and virulence were not detected from the genome analysis of this strain. Consistent with this, minimal inhibitory concentrations against various antibiotics, biogenic amines and D-lactate production, as well as enzymatic and hemolysis activities indicated that L. plantarum IDCC 3501 did not produce any harmful compounds during fermentation. Also, no acute toxicity and mortality were observed in a murine mouse model when feeding with L. plantarum IDCC 3501. Based on our findings, L. plantarum IDCC 3501 is safe and beneficial for human consumption.

Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

2019 ◽  
pp. 1-9
Author(s):  
Samira Salem ◽  
Essaid Leghouchi ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Time Course ◽  
Acute Inflammation ◽  
Lichen Species ◽  
Paw Edema ◽  
Sod Activity ◽  
Edema Formation ◽  
Lobaria Pulmonaria ◽  
Anti Inflammatory ◽  
Endogenous Antioxidant ◽  
Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

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