Profiling of influenza viruses by high-throughput carbohydrate membrane array

2011 ◽  
Vol 3 (3) ◽  
pp. 283-296 ◽  
Author(s):  
Weng-I Lao ◽  
Ya-Fang Wang ◽  
Yu-Dai Kuo ◽  
Chun-Hung Lin ◽  
Tsung-Chain Chang ◽  
...  
Keyword(s):  
High Throughput ◽  
Influenza Viruses ◽  
Membrane Array

scholarly journals Asymmetric synthesis of host-directed inhibitors of myxoviruses

2013 ◽  
Vol 9 ◽  
pp. 197-203 ◽  
Author(s):  
Terry W Moore ◽  
Kasinath Sana ◽  
Dan Yan ◽  
Pahk Thepchatri ◽  
John M Ndungu ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Asymmetric Synthesis ◽  
High Throughput ◽  
High Throughput Screening ◽  
Aqueous Solubility ◽  
Influenza Viruses ◽  
Lead Compound ◽  
Synthetic Strategy ◽  
Aqueous Solubilities ◽  
Nanomolar Range

High-throughput screening (HTS) previously identified benzimidazole 1 (JMN3-003) as a compound with broad antiviral activity against different influenza viruses and paramyxovirus strains. In pursuit of a lead compound from this series for development, we sought to increase both the potency and the aqueous solubility of 1. Lead optimization has achieved compounds with potent antiviral activity against a panel of myxovirus family members (EC50 values in the low nanomolar range) and much improved aqueous solubilities relative to that of 1. Additionally, we have devised a robust synthetic strategy for preparing 1 and congeners in an enantio-enriched fashion, which has allowed us to demonstrate that the (S)-enantiomers are generally 7- to 110-fold more potent than the corresponding (R)-isomers.

scholarly journals Subtyping of Swine Influenza Viruses Using a High-Throughput Real-Time PCR Platform

2018 ◽  
Vol 8 ◽  
Author(s):  
Nicole B. Goecke ◽  
Jesper S. Krog ◽  
Charlotte K. Hjulsager ◽  
Kerstin Skovgaard ◽  
Timm C. Harder ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Swine Influenza ◽  
Influenza Viruses

Crosstalk between adjacent nanopores in a solid-state membrane array for multi-analyte high-throughput biomolecule detection

2016 ◽  
Vol 120 (6) ◽  
pp. 064701 ◽  
Author(s):  
Muhammad Usman Raza ◽  
Sajid Saleem ◽  
Waqas Ali ◽  
Samir M. Iqbal
Keyword(s):  
Solid State ◽  
High Throughput ◽  
Biomolecule Detection ◽  
Membrane Array

scholarly journals Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Emilie Bonin ◽  
Stéphane Quéguiner ◽  
Cédric Woudstra ◽  
Stéphane Gorin ◽  
Nicolas Barbier ◽  
...  
Keyword(s):  
High Throughput ◽  
Swine Influenza ◽  
Influenza Viruses ◽  
High Throughput Analysis ◽  
Molecular Subtyping ◽  
Throughput Analysis

scholarly journals Development of a high-throughput membrane-array method for molecular diagnosis of circulating tumor cells in patients with gastric cancers

2006 ◽  
Vol 119 (2) ◽  
pp. 373-379 ◽  
Author(s):  
Chan-Hang Wu ◽  
Shiu-Ru Lin ◽  
Fang-Jung Yu ◽  
Deng-Chyang Wu ◽  
Yong-Sang Pan ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Molecular Diagnosis ◽  
High Throughput ◽  
Gastric Cancers ◽  
Membrane Array

scholarly journals A multiplex one-step real-time RT-PCR assay for influenza surveillance

2011 ◽  
Vol 16 (7) ◽  
Author(s):  
I Huber ◽  
H Campe ◽  
D Sebah ◽  
C Hartberger ◽  
R Konrad ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Influenza A ◽  
Multiplex Assay ◽  
Influenza Viruses ◽  
Influenza Surveillance ◽  
Influenza B ◽  
Rt Pcr ◽  
Influenza A H1n1 ◽  
One Step

For surveillance purposes real-time PCR assays for influenza viruses had to be adapted to the pandemic influenza A(H1N1)2009 strain. We combined published primers and probes for influenza A, influenza B and an internal amplification control with a detection system for influenza A(H1N1)2009 to set up a rapid, reliable, simple and cost-effective high-throughput multiplex one-step real-time RT-PCR. The workflow also includes automated sample preparation for high-throughput screening. The lower limit of detection of the multiplex assay was 3.5x102 RNA copies per PCR reaction. The diagnostic sensitivity of the multiplex assay was 87.7%, but increased to 99.4% for influenza-positive samples yielding Ct values of less than 34 cycles in the respective diagnostic assay. High specificity was confirmed by sequencing and correct detection of 15 reference samples from two quality assurance studies. The multiplex PCR was introduced for surveillance of samples from a network of general practitioners and paediatricians in Bavaria, Germany during the influenza pandemic of 2009. Comparison with surveillance data from reported cases proved the reliability of the multiplex assay for influenza surveillance programmes.

scholarly journals Modular glycosphere assays for high-throughput functional characterization of influenza viruses

2013 ◽  
Vol 13 (1) ◽  
pp. 34 ◽  
Author(s):  
Sven N Hobbie ◽  
Karthik Viswanathan ◽  
Ido Bachelet ◽  
Udayanath Aich ◽  
Zachary Shriver ◽  
...  
Keyword(s):  
High Throughput ◽  
Functional Characterization ◽  
Influenza Viruses

scholarly journals Use of hemagglutinin and neuraminidase amplicon-based high-throughput sequencing with variant analysis to detect co-infection and resolve identical consensus sequences of seasonal influenza in a university setting

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Temitope O. C. Faleye ◽  
Deborah Adams ◽  
Sangeet Adhikari ◽  
Helen Sandrolini ◽  
Rolf U. Halden ◽  
...  
Keyword(s):  
High Throughput ◽  
Seasonal Influenza ◽  
High Throughput Sequencing ◽  
De Novo ◽  
Influenza Viruses ◽  
H1n1 Vaccine ◽  
Consensus Sequences ◽  
Three Samples ◽  
Variant Analysis ◽  
Local Transmission

Abstract Background Local transmission of seasonal influenza viruses (IVs) can be difficult to resolve. Here, we study if coupling high-throughput sequencing (HTS) of hemagglutinin (HA) and neuraminidase (NA) genes with variant analysis can resolve strains from local transmission that have identical consensus genome. We analyzed 24 samples collected over four days in January 2020 at a large university in the US. We amplified complete hemagglutinin (HA) and neuraminidase (NA) genomic segments followed by Illumina sequencing. We identified consensus complete HA and NA segments using BLASTn and performed variant analysis on strains whose HA and NA segments were 100% similar. Results Twelve of the 24 samples were PCR positive, and we detected complete HA and/or NA segments by de novo assembly in 83.33% (10/12) of them. Similarity and phylogenetic analysis showed that 70% (7/10) of the strains were distinct while the remaining 30% had identical consensus sequences. These three samples also had IAV and IBV co-infection. However, subsequent variant analysis showed that they had distinct variant profiles. While the IAV HA of one sample had no variant, another had a T663C mutation and another had both C1379T and C1589A. Conclusion In this study, we showed that HTS coupled with variant analysis of only HA and NA genes can help resolve variants that are closely related. We also provide evidence that during a short time period in the 2019–2020 season, co-infection of IAV and IBV occurred on the university campus and both 2020/2021 and 2021/2022 WHO recommended H1N1 vaccine strains were co-circulating.

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