Melissopalynological Analysis of Forest Honey from North Malaysia

Honey is a natural product widely used by humans due to its sweet taste and health benefits produced by bees from nectar and honey dew of various plants. To establish and increase the production of honey one must know the plants that take part in the production of honey. In this study pollen analysis of forest honey samples from northern part of Malaysia was carried out to determine the botanical sources playing role in the production of honey in that region. The pollen samples were acetolyzed and identified microscopically. Out of the three samples studied Baling sample was unifloral having Mimosa scabrella as predominant pollen while Jeli and Gerik samples are multifloral containing Tipo myrcia and Elais guineensis as major secondary pollen. Fabaceae family represented four pollen types and accounted 80% of pollen in Baling sample and 34 % in Gerik sample while completely absent in Jeli sample. These results showed the dominance of plants from Fabaceae family in honey production. All the samples analysed have Albizia falcataria, Eupatorium sp., Sparganium typha, Tilia sp. and Tipo myrcia in common indicating that these plants are present in all the three places and these results also can be used as a tool in geographical identification of North Malaysian honey from others.

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Pollen Resources Stored in Nests of Wild Bees Xylocopa ciliata Burmeister and Megachile pusilla Pérez (Hymenoptera: Anthophila) in a Temperate Grassland-Forest Matrix

Sociobiology ◽

10.13102/sociobiology.v65i4.3470 ◽

2018 ◽

Vol 65 (4) ◽

pp. 784

Author(s):

Favio Gerardo Vossler

Keyword(s):

Pollen Analysis ◽

Diet Composition ◽

New World ◽

Temperate Grassland ◽

Wild Bees ◽

Three Samples ◽

Pollen Types ◽

Pollen Preference ◽

Origin Area ◽

Lotus Glaber

Pollen analysis was employed to study the diet composition of two wild bees in a patch of temperate grassland invaded by exotic plants. Thirty pollen types from 14 families and two unidentified types were present in the seven samples analyzed. The three samples from Megachile pusilla Pérez were composed of 100% Lotus glaber and the four samples from Xylocopa ciliata Burmeister of abundant pollen (˃5%) of Lotus glaber, Galega offi cinalis, Adesmia bicolor and type Senna-Chamaecrista (all Fabaceae). The latter resource likely foraged out of the park, which could be an evidence to support its pollen preference for Fabaceae. However, a larger number of samples is necessary to identify the specialization status of X. ciliata. The preference for Lotus in this site was due to temporal specialization as M. pusilla was identified as polylectic in its origin area, and this could help to explain its eff ective naturalization in the New World and other areas of the Old World.

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Pollen analysis in honey samples from the two main producing regions in the Brazilian northeast

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652007000300003 ◽

2007 ◽

Vol 79 (3) ◽

pp. 381-388 ◽

Cited By ~ 16

Author(s):

Geni da S. Sodré ◽

Luís C. Marchini ◽

Carlos A.L. de Carvalho ◽

Augusta C. de C.C. Moreti

Keyword(s):

Quantitative Analysis ◽

Pollen Analysis ◽

Sao Paulo ◽

The State ◽

São Paulo ◽

Pollen Type ◽

Northeastern Region ◽

Pollen Types ◽

Abundant Supply ◽

Brazilian Northeast

Knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of nectar and pollen for the bees, thus contributing toward improved yield. The present study means to identify the pollen types occurring in 58 samples of honey produced in two states of the northeastern region of Brazil, Piauí (38 samples) and Ceará (20 samples), and to verify the potential of the honey plants during the months of February to August. The samples were obtained directly from beekeepers in each state and analyzed at the Apiculture Laboratory of the Entomology Section of Escola Superior de Agricultura "Luiz de Queiroz", USP, Piracicaba, State of São Paulo, Brazil. The pollen analysis was performed using the acetolysis method. The samples were submitted to both a qualitative and a quantitative analysis. The dominant pollen in the State of Ceará is from Mimosa caesalpiniaefolia, M. verrucosa, Borreria verticillata, Serjania sp., and a Fabaceae pollen type, while in the State of Piauí it is from Piptadenia sp., M. caesalpiniaefolia, M. verrucosa, Croton urucurana and Tibouchina sp.

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Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia

Nusantara Bioscience ◽

10.13057/nusbiosci/n120203 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Lalu Zulkan Jayadi ◽

Ratna Susandarini

Keyword(s):

Stingless Bees ◽

Geographical Origin ◽

Botanical Origin ◽

Pollen Type ◽

Pollen Content ◽

Pollen Types ◽

Unifloral Honey ◽

Melissopalynological Analysis ◽

Lombok Island ◽

Number Of Plant Species

Abstract. Jayadi LZ, Susandarini R. 2020. Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia. Nusantara Bioscience 12: 97-108. Honey is a natural product with a variety of benefits that commonly used as food sweeteners, health supplements, and traditional medicine. There has been no comprehensive publication regarding the diversity of pollen contained in honey produced by stingless bee Tetragonula laeviceps and Heterotrogona itama from Lombok. This study aimed to reveal the diversity of pollen content in honey through melissopalynological analysis of honey samples produced by two species of stingless bees T. laeviceps and H. itama in Lombok Island. This melissopalynological study was performed on honey samples obtained from nine villages from nine sub-districts representing the three districts on Lombok Island. Pollen extraction from honey was carried out using standard methods for melissopalynological analysis. The diversity of pollen recovered from honey varied from 15 to 41 pollen types. Total number of plant species whose pollen was recovered from honey samples was 127 which consisted of 61 families. The occurrence of predominant pollen type in particular unifloral honey samples indicated their botanical origin and presumed geographical origin of honey. Results of this study are useful in confirming botanical origin of honey and generating information on plants potential as food source for sustainable beekeeping in Lombok Island.

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Comparative study of honeys and herbhoneys quality paramertes

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/72/1601 ◽

2017 ◽

pp. 117-120

Author(s):

Anna Novák ◽

Béla Kovács ◽

Nikolett Czipa

Keyword(s):

Comparative Study ◽

Positive Impact ◽

Health Benefits ◽

Sweet Taste ◽

Quality Parameters ◽

Nectar Source ◽

Relevant Regulation

Honey is one of the oldest known and consumed food. Besides the pleasant sweet taste it has been consumed because of the favorable health benefits. In addition to large number of honey plants there are many popular herbals which cannot serve as a nectar source. The herbhoney has been developed that consumers can joy the pleasant properties of herbals and honeys positive impact on the same product. In our research we compare the quality parameters of honeys and herbhoneys to each other and with the relevant regulation.

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Palynological Studies in Brigalow (Acacia harpophylla F. Muell.) Communities: Pollen Analysis in Gilgai Soil Under the Primary Forest

Australian Journal of Botany ◽

10.1071/bt9750355 ◽

1975 ◽

Vol 23 (2) ◽

pp. 355 ◽

Cited By ~ 7

Author(s):

WH Litchfield

Keyword(s):

Pollen Analysis ◽

Physical Stability ◽

Pollen Grains ◽

Primary Forest ◽

Eucalypt Forest ◽

Biological Attack ◽

Pollen Types ◽

History Of ◽

Successional Change ◽

Pollen Preservation

A pollen analysis of acid clays under brigalow is presented. The pollen fluxes in a first pair of soil profiles throw light upon the natural history of one locality as regards both its modern vegetation and the physical stability of gilgai. Fluxes in the relative pollen preservation indicate intensity of biological attack on the exine. In a profile representative of the higher level, preservation is good enough, erosion or fill on a micro-scale so slight, and any vertical down-wash so slow as to show up residual flux variations from successional change. But under this natural canopy, even though there is only a very slow wash of litter and soil down to the bottom of the depression, a faster tempo of decay than any in the higher ground, and physical incorporation down deeper cracks, maintain a balance that prevents accumulation on an almost bare surface. This shows up in deeper penetration of pollen grains, in even the residual pollen types being severely eroded, and in irregular fluxes in which the successional trace is lost. Presumably, old Myrtaceae types have washed out of the soil above. The pollen fluxes indicate a natural replacement of eucalypt forest by brigalow some time before clearing. The expanding fluxes at the immediate surface are taxa from outside the forest, particularly the Chenopodiaceae, that would have become more abundant after clearing. Insight into soil stability comes from the marked contrast in the distribution and the preservation of the pollen grains across the microrelief. The higher level is physically more stable than the bottom of the adjacent depression. Depressions are surprisingly dry in natural forest, but these pollen fluxes suggest that they are occas- ionally the wettest microenvironment.

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Comparative pollen preferences by africanized honeybees Apis mellifera L. of two colonies in Pará de Minas, Minas Gerais, Brazil

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652010000200005 ◽

2010 ◽

Vol 82 (2) ◽

pp. 293-304 ◽

Cited By ~ 15

Author(s):

Cynthia F.P. da Luz ◽

Gabriel L. Bacha Junior ◽

Rafael L.S. e Fonseca ◽

Priscila R. de Sousa

Keyword(s):

Apis Mellifera ◽

Major Part ◽

Minas Gerais ◽

Pollen Production ◽

European Standard ◽

Pollen Trap ◽

Three Samples ◽

Pollen Types ◽

Minas Gerais State ◽

Africanized Honeybees

The aim of this study was to investigate the polliniferous floral sources used by Apis mellifera (L.) (africanized) in an apiary situated in Pará de Minas, Minas Gerais state, and evaluate the pollen prefences among the beehives. Two beehives of Langstroth type with frontal pollen trap collectors were used. The harvest was made from September 2007 to March 2008, with three samples of pollen pellets colected per month per beehive. The subsamples of 2 grams each were prepared according to the European standard melissopalynological method. A total of 56 pollen types were observed, identifying 43 genus and 32 families. The families that showed the major richness of pollen types were: Mimosaceae (8), Asteraceae (6), Fabaceae (3), Arecaceae (3), Euphorbiaceae (3), Rubiaceae (3), Caesalpiniaceae (2), Moraceae (2) and Myrtaceae (2). The most frequent pollen types (> 45%) were Mimosa scabrella, Myrcia and Sorocea. The results demonstrated a similarity regarding the preferences of floral sources during the major part of the time. There was a distinct utilization of floral sources among the pollen types of minor frequency. In spite of the strong antropic influence, the region showed a great polliniferous variety, which was an indicative of the potential for monofloral as well as heterofloral pollen production.

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Pollen morphology of Pacific Northwestern Polemonium species in relation to paleoecology and taxonomy

Canadian Journal of Botany ◽

10.1139/b79-287 ◽

1979 ◽

Vol 57 (21) ◽

pp. 2428-2442 ◽

Cited By ~ 5

Author(s):

Rolf W. Mathewes

Keyword(s):

Light Microscopy ◽

Electron Micrographs ◽

Pollen Analysis ◽

Pollen Morphology ◽

Scanning Electron Micrographs ◽

Taxonomic Treatment ◽

Close Affinity ◽

Pollen Types ◽

Quaternary Paleoecology ◽

Scanning Electron

Six morphological types of Polemonium pollen are described, including P. micranthum which is the only taxon considered to be consistently identifiable to species. A key to the pollen types as seen in light microscopy is presented along with light and scanning electron micrographs of each type. The palynological evidence supports the taxonomic treatment of P. uciitiflorum and P. occidentale as subspecies of P. caeruleum, and supports a close affinity between P. pectinatum and P. foliosissimum. Brief summaries of the present-day ecology and distribution of Pacific Northwestern Polemonium species are presented. The application of indicator pollen analysis in Quaternary paleoecology is discussed, with special references to examples of paleoenvironmen-tal inference using Polemonium pollen types.

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Pollen analysis of Holocene sediments from the Poço das Antas National Biological Reserve, Silva Jardim, Rio de Janeiro, Brazil

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652008000300013 ◽

2008 ◽

Vol 80 (3) ◽

pp. 531-541 ◽

Cited By ~ 6

Author(s):

Luciane G. Coelho ◽

Ortrud M. Barth ◽

Dorothy S.D. de Araujo

Keyword(s):

Pollen Analysis ◽

Rio De Janeiro ◽

Palynological Analysis ◽

Fragmented Forest ◽

Pioneer Plants ◽

Three Samples ◽

Two Samples ◽

Bottom To Top ◽

Janeiro State ◽

Biological Reserve

The Poço das Antas National Biological Reserve is located in Rio de Janeiro State, southeast Brazil. This paper presents information on past environmental characteristics of the area through pollen analysis. Two sedimentary columns were collected and five samples were selected for radiocarbon dating. The following ages of the columns from bottom to top were detected: column 1 - 1.20-1.16 m: 6080 ±40 years BP, 0.775-0.735 m: 4090 ±40 years BP, 0.385-0.345 m: 1880 ±80 years BP; column 2 - 1.22-1.18 m: 3520 ±40 years BP, 0.23-0.19 m: 1810 ±40 years BP. Three samples from column 1 and two samples from column 2 were selected for pollen analysis: 1.20 m, 0.77 m and 0.37 m of column1 and 1.22 m and 0.21 m of column 2. Chemical treatment followed standard methodology. The palynological analysis shows that around 6080 years BP the study area was dominated by a rain forest and from around 4090 years BP the vegetation changed to a fragmented forest, restricted to low hills and surrounded by an open area of grassland and pioneer plants, swamps and peat areas. The pollen assemblage of the samples 3520, 1880 and 1810 years BP suggest the permanence of this kind of vegetation between 4000 years BP and the actual.

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Liking and Acceptability of Whole Grains Increases with a 6-Week Exposure but Preferences for Foods Varying in Taste and Fat Content Are Not Altered: A Randomized Controlled Trial

Current Developments in Nutrition ◽

10.1093/cdn/nzaa023 ◽

2020 ◽

Vol 4 (3) ◽

Author(s):

Angela De Leon ◽

Dustin J Burnett ◽

Bret M Rust ◽

Shanon L Casperson ◽

William F Horn ◽

...

Keyword(s):

Controlled Trial ◽

Health Benefits ◽

Sweet Taste ◽

Fat Content ◽

Whole Grains ◽

Mere Exposure ◽

Dietary Guidelines ◽

High Fat ◽

Low Fat ◽

Grain Products

ABSTRACT Background Since 2005, the Dietary Guidelines for Americans have recommended consuming at least half of total grains as whole grains (WGs) for optimal health benefits; however, consumption of WGs falls far short of recommended amounts. Objective This study aimed to evaluate the effect of mere exposure to WGs on liking, acceptability, and consumption of WG foods and to determine if exposure to WG would influence liking and wanting for other foods varying in fat content and sweet taste. Methods Healthy, self-identified low WG consumers (n = 45) were randomly assigned to either a 6-wk WG intervention or a refined grain (RG) control condition during which they received a weekly market basket of grain products to incorporate into daily meals and snacks. Consumption of grain products was measured by weekly logs and weigh-backs. A sensory evaluation protocol was conducted at baseline and week 6 to evaluate changes in perception of grain products. Computer tasks designed to measure liking and wanting for other foods varying in high/low-fat content and sweet/savory taste were also completed at baseline and week 6. Results Participants in the WG group significantly increased WG consumption. Exposure to WG products resulted in improved ratings of liking, flavor, texture, and willingness to include WG in the regular diet. No significant changes in liking or wanting for foods representing high-fat sweet (HFSW), low-fat sweet (LFSW), high-fat savory (HFSA), or low-fat savory (LFSA) categories were found in the WG group. In contrast, exposure to RG foods resulted in an increased explicit wanting for HFSW and LFSW and a decreased wanting for HFSA foods. Conclusions Mere exposure to WG foods represents a feasible and easily applied behavioral strategy for increasing consumption of WGs. Encouraging consumers to focus on enjoyment of the taste may be more effective than emphasizing the health benefits of WG consumption. This trial was registered at clinicaltrials.gov as NCT01403857.

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Análisis polinico de mieles de los Parques Naturales Los Alcornocales y Sierra de Grazalema.

Acta Botanica Malacitana ◽

10.24310/abm.v28i0.7268 ◽

2003 ◽

Vol 28 ◽

pp. 79-87

Author(s):

Anass Terrab ◽

Cristina Andrés ◽

María Josefa Díez

Keyword(s):

Light Microscopy ◽

Pollen Analysis ◽

Pollen Grains ◽

Class Ii ◽

Class V ◽

Natural Park ◽

Natural Parks ◽

Pollen Types ◽

Class 1

RESUMEN. Análisis polinico de mieles de los Parques Naturales Los Alcornocales y Sierra de Grazalema. Seha realizado el análisis microscópico de 7 muestras de miel de los Parques Naturales Los Alcornocales y Sierra de Grazalema. Las muestras fueron proporcionadas directamente por los apicultores. Los resultados reflejan que el nectar de las flores es la principal fuente de miel en el territorio y que una muestra pertenece a la Clase I de Maurizio, cinco a la Clase II y una a la Clase V, siendo el número de granos de polen en 10 gr. de miel entre 17.500 y 1.592.700. Se han identificado 41 tipos polínicos, perteneciendo a 17 familias botánicas, resultando dos mieles monoflorales: una de girasol y otra de eucalipto.Palabras clave. Girasol, Eucalipto, Melitopalinología, Parque Natural Los Alcornocales, Parque Natural Sierra de Grazalema.ABSTRACT. Pollen analysis of honeys from the Natural Parks Los Alcornocales and Grazalema Range. Seven honey samples from different localities of The Natural Parks Los Alcornocales and Grazalcma Range have been studied by light microscopy. The results show that the nectar from flowers is the main honey source in the region and that one sample belongs to the Maurizio Class 1, five to the Class II and one to the Class V, with 17.500-1.592.700 pollen grains per 10 honey gram. 41 pollen types were identified belonging to 17 families, and two honey samples are unifloral: one of sunflower and one of eucalyptus.Key words. Sunflower, Eucalyptus, Melissopalynology, Natural Park Los Alcornocales, Natural Park Grazalema Range.

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