scholarly journals Concentration of some heavy metals in the hair, kidney and liver of cattle and goats in the oil and non–oil producing areas of Ondo State

2021 ◽  
Vol 44 (3) ◽  
pp. 49-55
Author(s):  
G. O. Egigba ◽  
E. I. Odokuma ◽  
U. J. Ikhatua ◽  
M. A. Bamikole
Keyword(s):  
Significant Variation ◽  
Chromium Concentration ◽  
Anthropogenic Sources ◽  
Toxic Substances ◽  
Oil Exploration ◽  
Mean Values ◽  
Randomized Design ◽  
Regulatory Bodies ◽  
Ondo State ◽  
Range Test

Pollutions due to anthropogenic sources have made some animals possible carriers of certain toxic substances which could have detrimental effects on humans. In this study, the concentrations of lead (Pb), cadmium (Cd), mercury (Hg) and chromium (Cr) in the hair, kidney and liver of cattle and goats in the oil producing areas (OPA) and none oil producing areas (NOPA) of Ondo state, Nigeria, were evaluated. Hair, kidney and liver samples were collected from four purposely selected Local Government Areas in Ondo state in replicates, dried and digested using a 1:3 mixture of concentrated perchloric acid (HClO4) and trioxonitrate (v) acid (HNO3). Data collected were analyzed completely randomized design (CRD) and significant means were separated using Duncan multiple range test. Lead concentration (mg/kg) were not significantly different in the organs of cattle (mean = 0.076) and goats (mean = 0.043) in the OPA but varied significantly in the NOPA with highest values in the hair of cattle (0.081) and kidney of goat (0.056). Oil exploration effect was only significant in Pb deposition in the kidney. Cadmium values (mg/kg) ranged from 0.009 to 0.031 in cattle and from 0.010 to 0.020 in goats with no significant variation. However, oil exploration showed significant effect in the Cd deposition in the kidney of cattle. Mean values of mercury concentration (mg/kg) in cattle were between 0.022 to 0.054 and 0.029 to 0.039 in goats from the OPA. In the NOPA, Hg concentration did not vary significantly in the organs of cattle but showed significant variation in the organs of goats with values ranging from 0.016 – 0.046 mg/kg. Chromium concentration (mg/kg) varied between 0.004 to 0.020 in cattle and 0.005 to 0.025 in goats with no significant variation. Conclusively, all the heavy metal concentrations obtained from analysis of the hair, kidney and liver from both the oil producing and the non – oil producing areas were below permissible limits reported by some regulatory bodies.

scholarly journals Cytoprotective effects of garlic on spermatozoa quality and fertilizing ability of extended porcine semen

2020 ◽  
Vol 47 (5) ◽  
pp. 48-57
Author(s):  
O. A. Sokunbi ◽  
O. Alaba ◽  
B. Ogunwumiju ◽  
S. Eboh ◽  
T. Iruo
Keyword(s):  
Design Data ◽  
Mean Values ◽  
Randomized Design ◽  
Insémination Artificielle ◽  
Fertilizing Ability ◽  
Cytoprotective Agent ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Semen Extender ◽  
Range Test

Fertilizing potential of spermatozoa is pivotal for successful artificial insemination. Preservation of boar semen is associated with the production of reactive oxygen species which leads to decline of spermatozoa quality and decrease in fertilizing ability. The study aimed at investigating the effects of Ethanolic Allium sativum Extracts (EASE) as a cytoprotective agent on the quality and fertilizing potential of spermatozoa in extended boar semen. Semen was collected and aliquot portions were divided into 60 sample bottles comprising of five treatments with replicates. T1 served as the control (Beltsville Thawing Solution (BTS) + semen) in ratio 1:3 semen-extender, while other treatments T2 to T5 contained semen-extender at the same ratio, but supplemented with EASE at varying concentrations of 50, 75, 100 and 125 μg/L respectively stored at 17 °C and were evaluated at 0, 24, 48 and 72 hours for pH, normal spermatozoa (NS) (%), liveability (%), acrosome integrity (AI) (%) in a completely randomized design. Data obtained were subjected to descriptive statistics and analysed using one way ANOVA and means were separated using Duncan Multiple Range Test. Treated semen samples (75, 100 and 125 μg/L) gave superior (P<0.05) liveability than control, up to 72 hours. Similar (P>0.05) mean values were observed for NS from all treatments except 50 μg/L at 24 hours. At 72 hours, treated samples (75, 100 and 125 μg/L) gave superior (P<0.05) NS. Significant differences (P<0.05) in mean values for pH were observed among treatments through the extension period. However, all values were within accepted range for quality semen. Acrosome integrity were similar (P>0.05) among treatments from 0 to 72 hours, but reduced (P<0.05). Results suggest that the supplementation of BTS with EASE 125 μg/L, gave better cytoprotection to the spermatozoa compared to the control.     L'ablitédes spermatozoïdes de fertiliserest essentiel pour une insémination artificielle réussie. La conservation de la semence de l'our est associée à la production d'espèces réactives de l'oxygène, ce qui entraîne un déclin de la qualité des spermatozoïdes et une diminution de la capacité de fertilisation. L'étude visait à étudier les effets des extraits éthanoliques d'Allium sativum (EEAS) en tant qu'agent cytoprotecteur sur la qualité et le potentiel fertilisant des spermatozoïdes dans le sperme de sanglier étendu. Le sperme a été collecté et les portions aliquotes ont été divisées en 60 bouteilles d'échantillons comprenant cinq traitements avec des répliques. T1 a servi de contrôle (BeltsvilleThawing Solution (BTS) + sperme) dans un rapport 1: 3 sperme-extenseur, tandis que d'autres traitements T2 à T5 contenaient sperme-extenseur au même rapport, mais complété avec EEAS à des concentrations variables de 50, 75 , 100 et 125 μg / L respectivement conservés à 17 ° C et ont été évalués à 0, 24, 48 et 72 heures pour le pH, les spermatozoïdes normaux (SN) (%), l'habitabilité (%), l'intégrité de l'acrosome (IA) (%) dans une conception complètement aléatoire. Les données obtenues ont été soumises à des statistiques descriptives et analyses en utilisant une méthode ANOVA et les moyennes ont été séparées en utilisant le test Duncan Multiple Range. Les échantillons de sperme traités (75, 100 et 125 μg / L) ont donné une habitabilité supérieure (P <0.05) à celle des témoins, jusqu'à 72 heures. Des valeurs moyennes similaires (P> 0.05) ont été observées pour le SN pour tous les traitements, sauf 50 μg / L à 24 heures. A 72 heures, les échantillons traités (75, 100 et 125 ug / L) ont donné une SN supérieure (P <0.05). Des différences significatives (P <0.05) dans les valeurs moyennes du pH ont été observées entre les traitements pendant la période d'extension. Cependant, toutes les valeurs se situaient dans la plage acceptée pour le sperme de qualité. L'intégrité des acrosomes était similaire (P> 0.05) parmi les traitements de 0 à 72 heures, mais réduite (P <0.05). Les résultats suggèrent que la supplémentation en BTS avec EEAS 125 μg / L, a donné une meilleure cytoprotection des spermatozoïdes par rapport au témoin.

scholarly journals The use of coconut pulp as a feed substrate to methanogenesis inhibitor in in vitro rumen fluid fermentation

2017 ◽  
Vol 42 (3) ◽  
pp. 202
Author(s):  
E.H.B. Sondakh ◽  
J.A.D. Kalele ◽  
F.S. Ratulangi
Keyword(s):  
Microbial Activity ◽  
Rumen Fluid ◽  
Mean Values ◽  
Fatty Acids Profile ◽  
Feed Digestibility ◽  
Randomized Design ◽  
Methane Reduction ◽  
Range Test ◽  
Ruminant Feed

This experiment was conducted to determine the effect of the adding of coconut pulp in ruminant feed on methane reduction in vitro. Rumen fluid and substrate of forage and concentrate in ratio 60:40 were in this study. This experiment consisted of five treatments of coconut pulp as followed: R1: feed substrates without the coconut pulp; R2: substrate feed with coconut pulp 5%; R3: substrate feed with coconut pulp 10%; R4: Substrate feed with coconut pulp 15% and R5: substrate feed with coconut pulp 20%. Each treatment was conducted to four replications. Fermentation was done using the Hohenheim Gas Test (HGT) incubated at 39oC for 72 hours. At the end of fermentation was determined production of methane, parameters of fermentation, microbial activity, feed digestibility and fatty acids profile of the ruminal fluid. Data obtained were analyzed by using analysis of variance using completely randomized design. The differences of mean values were analyzed by Duncan multiple range test (DMRT). The results showed that methane production has decreased about 13.74%-17.39% when the feed was given coconut dregs 15-20%. Based on the results of the study was concluded that the supplementation of coconut pulp up to 15-20% of 100% dry matter can be used as a substrate of ruminant feed, because it can reduce methane and does not interfere the microbial activity in the fermentation of rumen fluid through in vitro. The supplementation of coconut pulp can be a referencein designing the methanogenic inhibitor feed.

scholarly journals Effect of captive rearing on meat and haemolymph nutritional characteristics of snail (Archachatina marginata swainson)

2021 ◽  
Vol 25 (9) ◽  
pp. 1677-1680
Author(s):  
I.E. Odiaka ◽  
A.S. Kehinde ◽  
O.O. Babatunde ◽  
T.A. Adebisi-Fagbohungbe ◽  
F.A. Abuldulazeez ◽  
...  
Keyword(s):  
Significant Variation ◽  
Mineral Elements ◽  
Land Snails ◽  
Randomized Design ◽  
Standard Procedures ◽  
Nutritional Characteristics ◽  
Nutritional Qualities ◽  
The Impact ◽  
Range Test ◽  
Captive Rearing

An experiment was conducted to investigate and compare the impact of treatment, which is the source of snail (wild and captive reared) on the proximate and mineral composition (Na, K, Ca, Mg, P, Fe and Mn) of the meat and haemolymph of African land snails (Archachatina marginata). Ninety (90) adult snails (250.00 + 0.5g) were randomly distributed into two (2) treatments, each of three replicates, (15 snails per replicate), in a Complete Randomized Design. Samples of meat and haemolymph collected using standard procedures were subsequently analyzed for their nutritional qualities, using standard chemical analytical procedures. All data collected were statistically analyzed using ANOVA, while significant variation were separated with Duncan Multiple Range Test of the same package. The proximate composition of the meat revealed higher (P>0.05) DM (26.00%), Ether extract (2.17%) and NFE (3.57%) in SC, while there was a non-significant variation (P<0.05) in most proximate factors for haemolymph, except NFE (4.27%), which was higher (P>0.05) in SW. The mineral profiling of the meat revealed  elevated (PP>0.05) levels of Fee (22.30mg/kg) and Mn (4.79mg/kg) of SC, the haemolymph recorded improved levels (P>0.05) for all mineral elements in SC. In conclusion, the captive rearing of snail had improved impact on thenutritional qualities of SC.

CONCENTRADO PROTEICO OBTIDO DAS FOLHAS DE MANDIOCA (Manihot esculenta Crantz) DE TRÊS VARIEDADES COMERCIAIS.

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Izabel Aparecida Soares ◽  
Mauro Sérgio Téo ◽  
Carlise DEBASTIANI ◽  
Suzymeire BARONI ◽  
Vanessa Silva RETUCI
Keyword(s):  
Manihot Esculenta ◽  
Crude Protein ◽  
Hydrocyanic Acid ◽  
Protein Concentrate ◽  
Manihot Esculenta Crantz ◽  
White Variety ◽  
Mean Values ◽  
Randomized Design ◽  
Significant Difference ◽  
The Mean

O trabalho teve por objetivo verificar diferenças entre rendimento do concentrado proteico e proteína bruta da folha da mandioca (Manihot esculenta Crantz), obtidos a partir de três variedades comerciais: branca, cascuda e vermelha. As manivas foram plantadas seguindo o delineamento experimental inteiramente casualizado com três repetições. Nas comparações entre as variedades, considerou coletas escalonadas pós-plantio, realizadas aos 12, 14 e 16 meses. O concentrado proteico foi obtido a partir da farinha das folhas inteiras e submetido ao método de termo - coagulação ácido e a proteína bruta pelo método padrão AOAC. Os dados foram submetidos a análise de variância e comparados pelo teste de Tukey - 5% de probabilidade. Os resultados não indicaram diferença significativa entre as médias obtidas para rendimento de concentrado proteico. Para a variável porcentagem de proteína bruta a variedade Branca foi a que apresentou maior valor, com média de 46,25%, seguida pela Cascuda e Vermelha, 44,52% e 37,30%, sucessivamente. Conclui-se que outros estudos devem avaliar condições que possam influenciar no teor de proteína foliar, como clima e solo, e, avaliar os níveis de ácido cianídrico de cada variedade, indicando qual das três é a melhor para a extração do concentrado proteico das folhas. Palavras chaves: Manihot esculenta Crantz, variedades comerciais, concentrado proteico, proteína bruta. ABSTRACT: The study aimed to assess the differences between income protein concentrate, crude protein of cassava leaf (Manihot esculenta Crantz), obtained from three commercial varieties: white, red and cascuda. The cuttings were planted following the completely randomized design with three replications. Comparisons between the varieties considered after planting staggered collections, held on 12, 14 and 16 months. The protein concentrate was obtained from flour of whole sheets and subjected to the term method - acid coagulation and crude protein by AOAC standard method. The data were submitted to ANOVA and Tukey test - 5% probability. The results indicated no significant difference between the mean values obtained for protein concentrate income. For the variable percentage of crude protein White variety showed the highest, with an average of 46.25%, followed by cascuda and Red, 44.52% and 37.30%, successively. We conclude that further studies should evaluate conditions that may affect the leaf protein content, such as climate and soil, and evaluate the hydrocyanic acid levels of each variety, indicating which of the three is the best for the extraction of protein concentrate from leaves Key words: Manihot esculenta Crantz, commercial varieties, protein concentrate, crude protein.

PENAMBAHAN ENZIM CAIRAN RUMEN LIMBAH RUMAH POTONG HEWAN PADA PAKAN BERBASIS WHEAT POLLARD DENGAN PROSES PENGOLAHAN PADA KOMPOSISI KIMIA PAKAN

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Dadik Pantaya., dkk
Keyword(s):  
Moisture Content ◽  
Research Data ◽  
Steam Temperature ◽  
Randomized Design ◽  
Durability Index ◽  
Feed Moisture ◽  
Completely Randomized Design ◽  
Enzyme Supplementation ◽  
Range Test ◽  
Basal Ration

The present study was conducted to clarify the effect of addition of enzymes from rumen liquor on performance broiler of pelleting feed with basal ration of wheat pollard with addition of enzymes and processing broiler diets. Ruminal enzymes were extracted from Australian Commercial Cross (ACC) cattles. Application of enzyme on wheat pollard. Enzyme was reacted with wheatpollard and mixed with another feedstuff before steam pelleting process. The combination nine experimental diets were used as follows three levels enzyme supplementation (0, 620 and 1.240 U/kg) and three levels steam temperature at (60, 80 and 100 oC) Feed particles size, hardness, PDI (Pellet Durability Index), moisture content, protein, GE and hemicellulosa were evaluated parameter devided into nine treatmens were used in this research. Data from Completely Randomized Design factorial 3 x 3 were analyzed with Anova, if differences were continued by Duncan’s new multiple range test. Enzyme supplementation decreased feed hardness and PDI. There were interaction beetwen enzyme and temperature for feed moisture content. Hemicellulosa was significantly reduced by steam temperature at 80 and 100 oC and enzyme supplementation 1.240 U/kgKey Words: enzyme liquor rumen, wheat pollard, steam pelleting, feed Chemical composition

Combination of Carbonated Aloe Vera Drink with Lemon Juice

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Jefrianta Demu Geri ◽  
Dewi Fortuna Ayu ◽  
Noviar Harun
Keyword(s):  
Vitamin C ◽  
Aloe Vera ◽  
Sweet Taste ◽  
Total Dissolved Solids ◽  
Lemon Juice ◽  
Dissolved Solids ◽  
Combination Treatments ◽  
Randomized Design ◽  
Yellow Color ◽  
Range Test

The purpose of this study was to determine the optimal ratio of carbonated aloe vera drink and lemon juice. This research used completely randomized design with four treatments and four replications. The treatments performed were L1 (90% carbonated aloe vera and 10% lemon juice), L2 (80% carbonated aloe vera and 20% lemon juice), L3 (70% carbonated aloe vera and 30% lemon juice), and L4 (60% carbonated aloe vera and 40% lemon juice). Data were statistically analyzed by using analysis of variance (ANOVA) and followed by duncan new multiple range test (DNMRT) at level 5%. The combination treatments of carbonated aloe vera drinks and lemon juice had a significant effect on acidity (pH), vitamin C, total dissolved solids, and descriptive and hedonic sensory assessment. The best treatment from the research was L3 (70% carbonated aloe vera and 30% lemon juice) with pH of 4.26, vitamin C of 19.97 mg, and total dissolved solids of 14.34°brix. The overall sensory test result preferred by panelists with descriptions of yellow color, lemon flavor, and had a slightly sweet taste.

PENGARUH SUBSTITUSI TEPUNG BUAH LINDUR (Bruguiera Gymnorrhiza) TERHADAP KARAKTERISTIK ORGANOLEPTIK, NILAI GIZI DAN AKTVITAS ANTIOKSIDAN BROWNIES

2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Wd Wd Sitti Aisyah Nurul Fahlani ◽  
Tamrin Tamrin ◽  
Kobajashi Togo Isamu
Keyword(s):  
Antioxidant Activity ◽  
Nutritional Value ◽  
National Standards ◽  
Bruguiera Gymnorrhiza ◽  
Nutritional Values ◽  
Organoleptic Characteristics ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test ◽  
Color Texture

ABSTRACT                     This study aimed to analyze the effect of lindur fruit flour substitution on organoleptic characteristics, nutritional value, and antioxidant activity of lindur fruit flour brownies. This research was conducted using a completely randomized design (CRD) with various percentages of formulation of substitution of lindur fruit flour:wheat flour, namely M1 = (100%:0%), M2 = (90%:10%), M3 = (80%:20 %), M4 = (70%:30%), and M5 = (60%:40%). The research data were analyzed using analysis of variance, with further testing using Duncan's Multiple Range Test (DMRT) at a 95% confidence level. The results show that the HCN content of lindur fruit flour reached 21.81 mg/kg. The M3 treatment was the most preferred treatment by the panelists with hedonic rating scores of color, texture, aroma, and taste reached 4.14 (like), 4.13 (like), 3.79 (like), and 4.30 (like), respectively. The nutritional values of selected M3 brownies show that it contained 30.57% water, 1.20% ash, 3.70% fat, 5.63% protein, and 58.90% carbohydrate. Analysis of antioxidant activity in selected M3 brownies was 465.58 ppm which is categorized as very weak. It can be concluded that the substitution of lindur fruit flour in making brownies had a very significant effect on color, texture, and taste. However, the effect was not significant on the aroma and the analysis of the nutritional value of the selected treatment. The brownie product substituted with lindur fruit flour met the national standards for water, ash, and fat contents.Keyword: Brownies, Lindur Fruit ABSTRAK    Penelitian ini bertujuan untuk mempelajari pengaruh substitusi tepung buah lindur terhadap karakteristik organoleptik, nilai gizi, dan aktivitas antioksidan brownies tepung buah lindur. Penelitian ini dilakukan dengan menggunakan Rancangan Acak  Lengkap  (RAL) dengan berbagai presentase formulasi, substitusi tepung buah lindur: tepung terigu yaitu M1 =  (100% : 0%), M2 = (90% : 10%), M3 = 80% : 20%), M4 = (70% : 30%), dan M5 = (60% : 40%). Data hasil penelitian dianalisis menggunakan sidik ragam (Analysis of Varian), dengan uji lanjut menggunakan Duncan’s Multiple Range Test (DMRT) pada taraf kepercayaan 95%. Hasil penelitian menunjukan analisis kadar HCN tepung buah lindur yaitu 21,81 mg/kg. Perlakuan M3 merupakan perlakuan yang paling disukai oleh panelis dengan skor penilain hedonik warna 4,14 (suka), tekstur  4,13 (suka), aroma 3,79 (suka) dan rasa 4,30 (suka). Analisis Nilai gizi produk browies tepilih M3 meliputi kadar air (30,57%), kadar abu (1,20), Kadar lemak (3,70%), kadar protein (5,63%) dan kadar karbohidrat (58,90%). Analisis aktivitas antioksidan pada brownies terplih M3 yaitu 465,58 ppm dengan kategori sangat lemah. Dapat disimpulkan bahwa substiusi tepung buah lindur pada pembuatan brownies berpengaruh sangat nyata terhadap warna, tekstur, dan rasa. Tetapi berpengaruh tidak nyata terhadap aroma. dan analisis nilai gizi perlakuan terpilih. Berdasarkan standar mutu SNI brownies, bahwa produk brownies substitusi tepung buah lindur sudah memenuhi standar mutu SNI untuk kadar air dan kadar abu serta kadar lemak.Kata kunci:   Brownies, tepung, Buah Lindur

scholarly journals One day-old chicks transport: Assessment of thermal profile in a tropical region

2015 ◽  
Vol 19 (7) ◽  
pp. 663-667 ◽  
Author(s):  
Aérica C. Nazareno ◽  
Iran J. O. da Silva ◽  
Frederico M. C. Vieira ◽  
Rofson F. S. Santos
Keyword(s):  
Thermal Environment ◽  
Kriging Interpolation ◽  
Specific Enthalpy ◽  
Tropical Region ◽  
Thermal Profile ◽  
Heterogeneous Distribution ◽  
Mean Values ◽  
Stressful Environment ◽  
Randomized Design ◽  
Data Loggers

The aim of this study was to assess the thermal profile of truck with different levels of box placement during one day-old chicks transport. An experiment was conducted through monitoring of 11 transport loads. A acclimatized truck was used in this research, with maximum capacity of 630 one day-old chicks boxes, totalizing 63,000 animals. The assessment of thermal environment was performed in 5 min intervals, through the following variables: temperature, relative humidity and specific enthalpy. The treatments were registered at two levels of the load (first rack and floor) where 17 data loggers were distributed throughout the truck. The experiment used a completely randomized design and geostatistics was used for spatial dependency and Kriging interpolation. The microclimatic conditions of the truck were not as per recommended values, which confirm a heterogeneous distribution of heat and moisture in environment. Regarding the box positioning, the mean values of thermal variables associated with thermal comfort of one day-old chicks was found in the floor area. The most stressful environment for birds inside the truck was located in front and at the center of the truck.

scholarly journals Genetic diversity and phylogenetic behavior of some minor legumes

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
James Oludare Agbolade ◽  
Ronke Justina Komolafe
Keyword(s):  
Genetic Diversity ◽  
Principal Component ◽  
Calyx Lobe ◽  
Descriptive Statistics ◽  
International Institute ◽  
Mean Values ◽  
Phylogenetic Relatedness ◽  
Petal Length ◽  
Morphological Differences ◽  
Range Test

Twenty-four accessions of twelve species minor legumes collected from the germplasm unit of the International Institute of Tropical Agriculture Ibadan, Nigeria were evaluated for their genetic diversities and phylogenetic relatedness. The accessions were planted into plots of 5 ridges of 5 meters long, spaced 1 meter apart and replicated three times at the Federal University Oye-Ekiti Teaching and Research Farm. The diversity and the relative phylogeny of the accessions were assessed through their floral morphological differences and the mean values between two accessions were evaluated by descriptive statistics. Principal component analysis was employed to identify the most discriminatory floral morphological traits and the similarities among the 24 accessions were assessed by cluster analysis (CA). Descriptive statistics through Duncan multiple range test adopted revealed genetic diversity and phylogenetic relatedness among the accessions. The first two principal component axes explained 64.66% of the total floral morphological variation. Standard petal length, calyx lobe length and stipule length contributed most of the variations in the legume accession. CA grouped the 24 accessions into six clusters. The study revealed intra-specific similarities and inter-specific floral morphological differences among the studied accessions.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

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