scholarly journals Comparative Hypolipidemic Evaluation of Aframomum Melegueta Seeds and Moringa Oleifera Leaves

2021 ◽  
pp. 166-182
Author(s):  
L. U. Nwankwo ◽  
F. A. Onyegbule ◽  
C. C. Abba ◽  
E. Agbamu
Keyword(s):  
Liver Function ◽  
Moringa Oleifera ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Negative Control ◽  
Lipid Lowering ◽  
Hypolipidemic Effect ◽  
Crude Extracts ◽  
Moringa Oleifera Leaves ◽  
Crude Drugs

Aims: The study was carried out to compare the lipid lowering effects of both crude drugs as well as deducing the extracts with the best lipid lowering property; and the fractions. Study Design: The research was conducted with an experimental design solely based on laboratory trials which involved the use of ninety-six (96) male albino wistar rats to compare the hypolipidemic effects of both crude drugs and respective fractions. Place and Duration of Study: Department of Pharmacognosy Laboratory, Faculty of Pharmacy, Delta State University, Abraka, Nigeria and Department of Pharmacology and Toxicology Laboratory, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Agulu, Anambra State, Nigeria. The research was carried out from March to August, 2021. Methodology: Ethanol extracts of both plants were prepared using soxhlet extraction. Each extract was then subjected to VLC fractionation using four solvents: n-hexane, chloroform, ethyl acetate and methanol. The fractions were bulked together after conducting thin layer chromatographic procedures and each extract was bulked into four fractions. The acute toxicity studies (LD50) of both extracts were determined in the rats using Lorke’s method. The crude extracts were screened for the presence and quantity of phytoconstituents using standard methods. The antilipidemic study was carried out using sixty-eight (68) rats randomized into seventeen (17) groups of four (4) animals each. Lipid profile was determined using spectrophotometer. Liver function tests and histology was also carried out using standard procedures. Results: Administration of various treatments (both crude extracts and fractions) evoked a significant (p<0.05) reduction of TC, TG, and LDL-C as well as significant (p<0.05) elevation of HDL-C when compared with the negative control. With a percentage serum lipid reduction of 45.11%TC, 48.23%TG, 63.39% LDL-C and 174.69% elevation of HDL-C, the group treated with the combination of 500 mg/kg Aframomum melegueta and 500 mg/kg Moringa oleifera produced the best hypolipidemic effect. This is closely followed by fraction MO4. Comparatively, Moringa oleifera extracts exerts a better antilipidemic effect than Aframomum melegueta seed extract. The liver function test showed that both plants has no toxic effect on the liver cells at doses of 250 mg/kg and 500 mg/kg, hence confirming the hepatoprotective effect of both crude drugs at the doses administered. Conclusion: In conclusion, results from this study suggests that ethanol extract of Moringa oleifera leaves is more effective than ethanol extract of Aframomum melegueta seeds as a                   hypolipidemic agent, however, combination of both crude drugs as a lipid lowering agent has proved to be more effective and reliable when compared to each crude drug administered independently.

scholarly journals EFEK EKSTRAK ETHANOL DAUN KELOR (MORINGA OLEIFERA) TERHADAP PROTEKSI FUNGSI HATI DAN HISTOPATOLOGI TIKUS PUTIH (RATTUS NORVEGICUS) YANG DIINDUKSI KARBONTETRAKLORIDA (CCL4)

2021 ◽  
Vol 21 (1) ◽  
pp. 177-185
Author(s):  
Siti Hardiyanti Nawir ◽  
Peter Kabo ◽  
Ilhamjaya Pattelongi
Keyword(s):  
Liver Function ◽  
Rattus Norvegicus ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Blood Chemistry ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Moringa Oleifera Leaves ◽  
One Way Anova

Abstrak Kerusakan hati menjadi salah satu penyakit dengan prevalensi yang tinggi khususnya di negara berkembang. Penelitian ini bertujuan untuk melihat adanya efek ekstrak ethanol daun kelor (Moringa oleifera) terhadap proteksi fungsi hati dan histopatologi tikus putih (Rattus norvegicus) yang diinduksi karbontetraklorida (ccl4). serta menentukan dosis efektif dari kombinasi tersebut. Penelitian ini dilakukan secara eksperimental laboratorium menggunakan metode eksperimen dengan desain post test only control group pada tikus wistar. Tikus dibagi menjadi 5 kelompok, yaitu kelompok kontrol positif (ekstrak kurkuma), kontrol pembanding (NaCMC 1%) dan 3 kelompok pemberian ekstrak dengan 3 variasi dosis masing-masing 250 mg/kgBB, 500 mg/kgBB dan 750 mg/kgBB. Dilakukan pengamatan terhadap uji profil kimia darah yang meliputi SGOT dan SGPT serta dilanjutkan dengan uji histopatologi. Hasil menunjukkan bahwa adanya perbedaan perubahan hasil pengukuran darah fungsi hati (SGOT dan SGPT) antar kelompok selama 8 hari. Hasil menunjukkan bahwa adanya perbedaan perubahan hasil pengukuran profil kimia darah antar kelompok selama perlakuan. Berdasarkan hasil uji one way ANOVA pada pengukuran SGOT dan SGPT terdapat hasil tes bermakna (P<0,05). Hasil uji histopatologi menunjukkan bahwa terdapat perbaikan fungsi dan struktur hati pada kelompok pemberian ekstrak etanol daun kelor. Dapat disimpulkan bahwa ekstrak etanol daun kelor (Moringa oleifera) memiliki kemampuan sebagai hepatoprotektor terhadap fungsi hati pada dosis 750 mg/kgBB. Abstract Liver damage is one of the diseases with high prevalence especially in developing countries. This study aims to see the effect of ethanol extract of Moringa oleifera leaves on liver function protection and histopathology of white rat (Rattus norvegicus) induced by carbontetrachloride (CCl4). and determine the effective dose of the combination. This research was conducted experimentally using a laboratory experimental method with a post test only control group design in wistar rats. Rats were divided into 5 groups, namely the positive control group (curkuma extract), comparative control (NaCMC 1%) and 3 groups giving extracts with 3 variations of each dose of 250 mg / kgBB, 500 mg / kgBB and 750 mg / kgBB. Observations were made on the blood chemistry profile test which included SGOT and SGPT and continued with histopathological testing. The results showed that there were differences in changes in the results of liver function blood measurements (SGOT and SGPT) between groups for 8 days. The results showed that there were differences in changes in the measurement results of blood chemistry profiles between groups during treatment. Based on the results of the one way ANOVA test on SGOT and SGPT measurements there were significant test results (P <0.05). The results of histopathological tests showed that there was an improvement in liver function and structure in the group given ethanol extract of kelor leaves. It can be concluded that the ethanol extract of Moringa oleifera leaves has the ability as a hepatoprotector of liver function at a dose of 750 mg / kgBB.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

scholarly journals Efek Antidiare Ekstrak Etanol Daun Kelor (Moringa oleifera L.) Pada Mencit Putih Jantan

2020 ◽  
Vol 6 (1) ◽  
pp. 35-39
Author(s):  
Rizal Fauzi ◽  
◽  
Annisa Fatmawati ◽  
Emelda Emelda ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Intestinal Motility ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Extraction Process ◽  
Negative Control ◽  
Control Group ◽  
Average Value ◽  
Normal Individuals

Diarrhea is an increase frequency and decrease consistency of fecal when compared with the bowel condition of normal individuals. The high incidence of acute and chronic diarrhea encourages researchers to keep trying to find new antidiarrheal drugs, especially from plants. The leaves of Moringa (Moringa oleifera Lam) contain tannins which are polyphenolic compounds and has benefits as anantidiarrheal.This study aims to determine the activity of the ethanol extract of moringa leaves as an antidiarrheal by looking at intestinal motility. The extraction process was carried out by maceration method using ethanol 70%..The result research is the average value of the treatment ratio is smaller than the negative control group. The average value of the ratio of the group was given by ethanol extract of moringa leaves at a dose of 9.1 mg / 20 g body weight was 0.664; ethanol extract of moringa leaves at dose of 18.2 mg / 20 g body weight was 0.434 and ethanol extract of moringa leaves at dose of 36.4 mg / 20 g body weight was 0.389. From this value, it is known that the ethanol extract of Moringa leaves has an antidiarrheal effect by inhibiting intestinal motility. The effect of the ethanol extract of Moringa leaves in inhibiting intestinal motility is best at a dose of 36.4 mg / 20 g body weight with an average value ratio of 0.389.

scholarly journals The Haematological Profiles of High Fat Diet Mice Model with Moringa oleifera Leaves Ethanol Extract Treatment

2019 ◽  
Vol 12 (04) ◽  
pp. 2143-2149
Author(s):  
Fenty Alia ◽  
Mas Rizky A. A Syamsunarno ◽  
Vanessa Ayu Sumirat ◽  
Mohammad Ghozali ◽  
Nur Atik
Keyword(s):  
High Fat Diet ◽  
Moringa Oleifera ◽  
Hematological Parameters ◽  
Ethanol Extract ◽  
Haematological Parameters ◽  
Hematological Parameter ◽  
Standard Diet ◽  
Low Grade ◽  
High Fat ◽  
Moringa Oleifera Leaves

Characteristics of obesity as dysfunction of adipose tissue and chronic low-grade inflammation can have impacts to haematological parameters. The Moringa oleifera leaves have been used empirically to treat metabolic and blood-related diseases. However, it is still unknown whether Moringa oleifera leaves can influence haematological parameters in high fat diet. The study aimed to investigate the hematological parameter of high-fat diet mice in parallel with Moringa oleifera leaves ethanol extract (MOLE). Forty male DDY mice in 5 weeks of age were randomly divided into five groups as follows: The SD group was fed with a standard diet, the HFD group was fed with high fat diet, the HFD+S group was fed with high-fat diet and simvastatin (0,8 mg/20gBW/day). The HFD+MOLE1 and the HFD+MOLE2 groups were fed with high-fat diet and MOLE in a dose of 5,6 mg/20gBW/day and 11,2 mg/20gBW/day, respectively. The experiment was performed for 7 weeks. At the end of the experiment, blood was drawn for haematology analysis of blood parameters. We found that sub-chronic high fat diet might alter the haematological parameters. Two different doses of MOLE might have potencies to prevent the worsening caused by those alterations, by increased of haemoglobin [12,9(10,4-15,3) vs 13,9(12,9-18,2) and 14(10,2-14,8), p>0,05)], slight decreased of WBC [(3,59±1,974) vs (3,433±1,747) and (3,42±1,014), p>0,05]. Additionally, the MOLE might have an effect to decreasing the granulocyte percentage [18,5(9,2-43,6) vs 11,5(7,6-18,2) and 8,05(3,2-25,1), p>0,05], and slight decreased of MPV [4,2(2,6-6,7) vs 3,6(3,2-4,1) and 3,55(2,6-6,8), p>0,05]. In summary, the study showed that MOLE might have potencies to prevent the worsening of hematological parameters in the sub-chronic high fat diet.

Anti-Bacterial from Moringa oleifera Lamk Crude Extracts

2014 ◽  
Vol 1060 ◽  
pp. 223-226
Author(s):  
Perayot Pamonsinlapatham
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Moringa Oleifera ◽  
Soxhlet Extraction ◽  
Stem Bark ◽  
Bacterial Activity ◽  
Diffusion Method ◽  
Crude Extracts ◽  
Agar Diffusion Method

Moringa oleiferaLamk (Moringaceae) is a worldwide use for food and traditional medicine. In this study, plants were separated into seven parts: 1) Leaf, 2) Drum, 3) Branching, 4) Seed, 5) Outer stem, 6) Stem bark, and 7) Root, respectively. Then, they were extracted by two different methods: methanol soxhlet extraction and ethanol maceration. All crude extracts were tested for anti-bacterial activity by agar diffusion method and colorimetric micro-dilution plate technique. The results showed that 7 parts of Moringa crude extracts had low anti-bacterial activity againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawhen compared with standard antibiotic (ampicillin or gentamycin). In conclusion, anti-bacterial effects ofM. oleiferacrude extracts had low anti-bacterial activityin vitrotest.

scholarly journals Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 112
Author(s):  
Theresia H. Tunas ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Moringa Oleifera Leaves ◽  
Inhibitory Power ◽  
Moringa Oleifera Lam

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.

Studies on neuropharmacological profile of ethanol extract of Moringa oleifera leaves in mice

2013 ◽  
Vol 149 (3) ◽  
pp. 783-789 ◽  
Author(s):  
Adewale G. Bakre ◽  
Adegbuyi O. Aderibigbe ◽  
Olusegun G. Ademowo
Keyword(s):  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Moringa Oleifera Leaves

The Effect of Moringa Oleifera Extract on Sperm Quality and Quantity of Diabetes Wistar Rat

2021 ◽  
Vol 2 (1) ◽  
pp. 91-96
Author(s):  
Novita Dwi Febrianty ◽  
Herisa Putri Anggraini ◽  
Meldawati ◽  
Boyke Marthin Simbolon
Keyword(s):  
Moringa Oleifera ◽  
Sperm Quality ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Chronic Disorder ◽  
Moringa Oleifera Leaves

A B S T R A C TDiabetes mellitus is a condition in which blood glucose levels increase in aperson's body which can result in chronic disorder. The effect of diabetes on amale reproductive system can affect sperm quality and disrupt the spermatogenesisprocess. Moringa oleifera leaves are rich in antioxidants such as flavonoids, vitaminA, vitamin E, and vitamin C, and contain selenium which helps lower bloodssugarlevels. This study was conductedtto determine the effect of administering Moringaoleifera leaf extract on the quality andiquantity ofssperms in Wistar rats with diabetesmellitus. This research is an experimental studyiusing pre-andipost-randomized,controlled group design. This study examinediblood sugarllevels, density,motility, andiviability ofssperm cells. This experimental test was dividediinto 4groups: one as a control group andtthree groups as treatment groups inducedwith alloxan and given ethanol extract of Moringa leaves with doses of 250, 500,and,750mg/kgBB. The results showed that the ethanol extract of Moringa leavesshowedssignificant improvements in various doses (P-value ≤ 0.05).

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