Multispectral Imaging Using Fluorescent Properties of Indocyanine Green and Methylene Blue in Colorectal Surgery—Initial Experience

Introduction: Image-guided surgery is becoming a new tool in colorectal surgery. Intraoperative visualisation of different structures using fluorophores helps during various steps of operations. In our report, we used two fluorophores—indocyanine green (ICG), and methylene blue (MB)—during different steps of colorectal surgery, using one camera system for two separate near-infrared wavelengths. Material and methods: Twelve patients who underwent complex open or laparoscopic colorectal surgeries were enrolled. Intravenous injections of MB and ICG at different time points were administered. Visualisation of intraoperative ureter position and fluorescent angiography for optimal anastomosis was performed. A retrospective analysis of patients treated in our departments during 2020 was performed, and data about ureter injury and anastomotic site complications were collected. Results: Intraoperative localisation of ureters with MB under fluorescent light was possible in 11 patients. The mean signal-to-background ratio was 1.58 ± 0.71. Fluorescent angiography before performing anastomosis using ICG was successful in all 12 patients, and none required a change in position of the planned colon resection for anastomosis. The median signal-to-background ratios was 1.25 (IQR: 1.22–1.89). Across both centres, iatrogenic injury of the ureter was found in 0.4% of cases, and complications associated with anastomosis was found in 5.5% of cases. Conclusions: Our study showed a substantial opportunity for using two different fluorophores in colorectal surgery, whereby the visualisation of one will not change the possible quantification analysis of the other. Using two separate dyes during one procedure may help in optimisation of the fluorescent properties of both dyes when using them for different applications. Visualisation of different structures by different fluorophores seems to be the future of image-guided surgery, and shows progress in optical technologies used in image-guided surgery.

Fluorescence-Aided Identification Technique (FIT) Improves Tooth Surface Clean-Up after Debonding of Buccal and Lingual Orthodontic Appliances

The aim of this study was to evaluate the use of fluorescence inducing light to aid the clean-up of tooth surfaces after bracket removal when using buccal or lingual orthodontic appliances. Two full sets of dental arches using extracted human teeth were assembled, with 14 teeth per arch. All teeth were bonded on their buccal and lingual surfaces. After debonding, a single blinded operator performed the tooth surface clean-up, as commonly performed in clinical practice; without the use of fluorescent light (non-FIT) and with two methods using fluorescent light to identify composite remnants on the tooth surfaces (FIT; OPAL and BRACE). Tooth surfaces were scanned before bonding and after clean-up, and the two scans were superimposed using the best-fit method. The results showed that the debonding method, type of tooth and type of tooth surface had a significant effect on the presence of composite remnants, enamel defects, and on debonding time. Contrary to the non-FIT method, there were no composite remnants after clean-up with the use of fluorescence inducing light. Clean-up time was significantly reduced on the buccal surfaces when using the FIT methods. On the lingual surfaces, the FIT methods resulted in larger enamel defects.

Effects of Imunit Insecticide on Biological Characteristics and Life Table Parameters of Spodoptera cilium (Lepidoptera: Noctuidae)

Imunit is a mixture of alpha-cypermethrin + teflubenzuron, and has been launched for controlling caterpillars. In this study, the effects of Imunit at LC50 and LC30 were investigated on parental and offspring generation of S. cilium, according to age-stage, two-sex life table. The experiments were conducted by leaf dipping method at 25 °C and 60 ± 5% relative humidity, under a cycle of 16 h fluorescent light and 8 h darkness. LC30 and LC50 concentrations of Imunit increased the immature developmental time of S. cilium in the offspring generation, while the LC50 of Imunit significantly reduced the developmental time of adults. The adult pre-oviposition period and total pre-oviposition period considerably increased when offspring were treated with LC50 of Imunit. In offspring of S. cilium exposed to LC50 and LC30 concentrations of Imunit, the gross reproductive rate (GRR), net reproduction rate (R0), the intrinsic rate of population increase (r), and the finite rate of population increase (λ) significantly reduced compared to the control. This study showed that the application of Imunit at LC50 could suppress the S. cilium population and can be used in the integrated management program of this pest.

Improved Oxidative Stability and Sensory Quality of Beef Hamburgers Enriched with a Phenolic Extract from Olive Vegetation Water

This study aims at evaluating the effect of a phenol-rich extract obtained from the concentration and purification of olive mill wastewaters (added at a ratio of 87.5 and 175 mg of phenols/kg meat) on the stability and sensory quality of beef hamburgers packed under modified atmosphere and stored under alternating exposure to fluorescent light at 4 ± 2 °C for 9 days. The hamburgers were sampled at different times (0, 6, and 9 days) and grilled at 200 °C. After 9 days, more than 56% of the added phenols in the raw burgers and more than 20% the grilled ones were retained. The results show that both concentrations of phenolic extract proved to effectively reduce primary and secondary lipid oxidation, as well as cholesterol oxidation products (COPs), during the shelf-life of raw hamburgers. Peroxide value, thiobarbituric acid reactive substances, and total COPs were up to 1.4-, 4.5-, and 8.8-fold lower in phenol-enriched raw hamburgers, respectively, than in the control samples; a similar trend was noted also in phenol-enriched cooked hamburgers (1.3-, 5.7-, and 4-fold lower). The sensory analysis also confirmed the effectiveness of the addition of phenolic extract, resulting in a positive effect on the red color intensity (raw product) and thus reducing browning during storage.

AN OVERVIEW OF IMPORTANT FACTORS TO CONSIDER WHEN CALIBRATING LEDS IN PHOTOMETRY WITH DIFFERENT DETECTORS

LED-based lighting products are the fastest developing light sources on the general lighting market. These include not only white light sources, but also coloured light sources. SI-traceable calibrations with a low measurement uncertainty are challenging because their unique spectral and geometric properties must be taken into account. The requirements on the measuring equipment and resulting uncertainty contributions differ significantly from those for the calibration of conventional incandescent or fluorescent light sources. Furthermore, different detector types can be used when calibrating LEDs. These include classic photometers, radiometers, spectroradiometers but also imaging systems such as ILMDs. Each of these measuring devices has its specific advantages and disadvantages. In this paper, traceable calibrations on 9 coloured and 9 white LEDs are discussed. Various array spectroradiometers, photometers, radiometers and a double monochromator were used for this purpose. Each of these measuring instruments is discussed in terms of its suitability for measuring different physical quantities (e.g. array spectrometer for measuring illuminance).

Quantum yield optimization of carbon dots using response surface methodology and its application as control of Fe3+ ion levels in drinking water

Early detection of heavy metals in drinking water is a fundamental step that must be taken to prevent adverse effects on health. This research aims to develop a heavy metal ion detector by utilizing the fluorescence properties of carbon dots. Cdots were synthesized using the microwave irradiation method based on the central composite design: urea mass 0.31-3.68 gr; reactor power 200-1000 W; synthesis time is 13-46 min, and the response is quantum yield. Material characterization includes PL, TEM, UV-VIS, XRD, and FTIR. The selectivity and sensitivity of Cdots as detectors were tested for Ag+, Bi3+, Ni2+, Al3+, Co2+, Pb2+, Fe3+, Zn2+, Zr4+, and Hg2+ ions at concentrations of 0-10 µM. The results showed that Cdots were successfully synthesized by fluorescent light green at 544 nm. An adequate response model is quadratic with the formulation QY= +58.36+10.41X1+14.06X2+13.59X3–5.57X2X3–4.89X12-8.60X22– 5.40X32. The best Cdots were obtained in the formulation of R9 (3 g, 800 W, 40 min), which resulted in a QY of 74.39%. The characteristics of Cdots are spherical, diameter 6.6 nm, the bandgap of 2.53 eV, and having an amorphous structure. The surface of Cdots contains various functional groups such as O-H, C-H, C=O, C N, and C=C. In the heavy metal detection test, Cdots showed specific sensitivity to Fe- 3+ ions. The addition of Fe3+ concentration and the extinction of Cdots fluorescence intensity formed a linear correlation F0/F=0.08894[Fe3+]+0.99391 (R2=0.99276). The detection ability of Cdots for Fe3+ ions reaches a concentration of 0.016 ppm, much lower than the regulatory threshold limit of SNI, WHO, and IBWA. The detection of Fe3+ ions in drinking water uses a fluorescence technique consistent with the SSA and ICP-OES. Based on these results, the fluorescence technique using Cdots can be an instrument for quality control of the final drinking water product.

Comparing Light—Emitting—Diodes Light Traps for Catching Anopheles Mosquitoes in a Forest Setting, Western Thailand

Light traps are a common method for attracting and collecting arthropods, including disease vectors such as mosquitoes. Various types of traps have been used to monitor mosquitoes in a forest in Western Thailand. In this study, four Light Emitting Diodes (LED) light sources (UV, blue, green, and red) and two fluorescent lights (white and UV) were used to trap nocturnal adult mosquitoes. These traps were used with light alone and not any additional attractant. The experiment was conducted from 18:00 to 06:00 h. on six consecutive nights, every two months, across dry, wet, and cold seasons. All specimens were first identified by morphological features and subsequently confirmed by using PCR. We collected a total of 873 specimens of 31 species in four genera, Anopheles, Aedes, Culex, and Armigeres. Anopheles harrisoni was the predominant species, followed by Aedes albopictus, Culex brevipalpis, Culex nitropunctatus, and Armigeres (Leicesteria) longipalpis. UV fluorescent light was the most effective light source for capturing forest mosquitoes, followed by UV LED, blue LED, green LED, white fluorescent, and red LED. The optimal times for collection were from 21:00 to 03:00 h in the dry season. Our results demonstrate that appropriate sampling times and light sources should be selected for optimal efficiency in vector surveillance programs.

Perovskite PV Energy Harvesting System for Uninterrupted IoT Device Applications

The performance of perovskite solar cells (PSCs) has been improved throughout the years. These photovoltaic (PV) cells can be used to power Internet of Things (IoT) devices for indoor applications. A perovskite PV energy harvesting system with a stand-by battery that continuously powers an IoT device is developed in this work. The battery is required to complement the PSCs when the latter have difficulties in power delivery during low or no irradiance. The performance of the energy harvesting circuit as well as the battery charge and discharge scenarios are investigated. Voltage matching between the PSC and the battery is achieved by a boost converter. The PSC energy harvesting system uses fractional open-circuit voltage (FOCV) based maximum power point tracking (MPPT), which utilizes a Sample and Hold (S&H) circuit. The FOCV technique is based on a comparison of the perovskite PV open circuit voltages and the maximum power points. For each irradiance level, the maximum power point is unique, and this work uses a light-dependent resistor (LDR) to adjust the scaling constant in MPPT. Case studies include various scenarios under 1000 lux fluorescent light and 1 sun irradiance as well as a consideration of different battery states.

Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings

Varying the spectral composition of light is one of the ways to accelerate the growth of conifers under artificial conditions for the development of technologies and to obtain sustainable seedlings required to preserve the existing areas of forests. We studied the influence of light of different quality on the growth, gas exchange, fluorescence indices of Chl a, and expression of key light-dependent genes of Pinus sylvestris L. seedlings. It was shown that in plants growing under red light (RL), the biomass of needles and root system increased by more than two and three times, respectively, compared with those of the white fluorescent light (WFL) control. At the same time, the rates of photosynthesis and respiration in RL and blue light (BL) plants were lower than those of blue red light (BRL) plants, and the difference between the rates of photosynthesis and respiration, which characterizes the carbon balance, was maximum under RL. RL influenced the number of xylem cells, activated the expression of genes involved in the transduction of cytokinin (Histidine-containing phosphotransfer 1, HPT1, Type-A Response Regulators, RR-A) and auxin (Auxin-induced protein 1, Aux/IAA) signals, and reduced the expression of the gene encoding the transcription factor phytochrome-interacting factor 3 (PIF3). It was suggested that RL-induced activation of key genes of cytokinin and auxin signaling might indicate a phytochrome-dependent change in cytokinins and auxins activity.

Advanced oxidation of high concentrations of formaldehyde in aqueous solution under fluorescent and UV light

Background: Formaldehyde (FA) is a carcinogen compound, which is soluble in water. FA can be removed from aqueous solution using advanced oxidation methods. Methods: In this study, the oxidation of FA was studied under fluorescent and UV light. Hydrogen peroxide (H2 O2 ) was used as an oxidant. The pH value and H2 O2 amount of samples were optimized. The chemical oxygen demand (COD), FA concentration, and H2 O2 consumption were followed. Results: It was observed that the pH value of the sample was more significant under fluorescent light than UV light at oxidation of FA. The highest COD removal and H2 O2 reduction were 18.57 and 30.90%, respectively, at pH 12.00, with a 1:1 ratio of FA:H2 O2 under fluorescent light. 86.41% FA and 62.12% COD removal were achieved at pH 7.00, with a 1:2 ratio of FA:H2 O2 , under UV light. It was observed that H2 O2 was consumed at all pH values under UV light. On the other hand, under fluorescent light, the concentration of H2 O2 decreased only at pH 12. In control samples containing only H2 O2 , the H2 O2 reduction was 92.09% at pH 11.91, while it was 2-6% at other pH levels under fluorescent light. The H2 O2 reduction changed between 33 and 44% at different pH values under UV light. The oxidation of FA was found to be suitable for the pseudo-first-order kinetic model and Langmuir isotherm model. Conclusion: The most effective oxidation was obtained at the original pH value (7.00) and 1:2 ratio of FA:H2 O2 under UV light.