Study on the splitting by hot-air drying of Camellia oleifera fruit

In order to explore the feasibility of hot air splitting of Camellia oleifera fruit, the effect of hot air temperature on peel splitting, the moisture state and moisture migration in peel, the peel microstructure and the seed color were studied. The results showed that higher hot air temperature could accelerate the splitting rate, the optimum temperature for splitting C. oleifera fruit was 90–110 °C considering the seed quality. Page model was the most suitable for describing the drying kinetic characteristics of C. oleifera fruit. Nuclear magnetic resonance (NMR) revealed the changing of the dehydration rate, the migration rate of bound water, immobilized water and free water in peel during hot air drying. The expansion of micro-channels in peel was conducive to moisture migration in the early splitting stage, but microstructure damaged in the late splitting stage accompanied by loose disorder of micro pores, serious shrinkage and deformation of peel.

Genetic and QTL Analysis for Hilum-Eye Types in Cowpea (Vigna Unguiculata L. Walp)

Cowpea is an important food legume widely grown in the semi-arid tropics and serves as a main source of dietary protein, minerals, and vitamins. However, varieties differ from region to region based on the consumer’s preference for seed types determined by seed size, seed coat texture, seed color, and hilum-eye types. The genetics of seed size, seed color, and seed coat texture have been well documented, but the hilum-eye types have not been studied well because they represent seven different types with complex interactions. We studied the genetic segregation for hilum-eye types and determined the number of genes involved in a recombinant inbred line (RIL) population derived from a cross between a small eye parent ‘GEC’ and a Watson eye parent ‘IT98K-476-8’. The results demonstrated a three-gene model, W (Watson), S (small), and R (large), for cowpea seed hilum-eye type pattern and the interaction of these three genes, W, S, and R, resulted in five phenotypes, viz. self, Watson, small, large, and ring hilum-eye types. Moreover, we also mapped the RILs for hilum-eye types, identified three quantitative trait loci (QTLs), and aligned to the cowpea reference genome as QTL qHilum7.1, qHilum9.1, and qHilum10.1, corresponding to these three genes, Ring type (R), Watson type (W), and Small type (S) hilum-eye type patterns, respectively. Therefore, there was a complete agreement between the genetic analysis and QTL mapping for the number of genes controlling the hilum types in cowpea.

Evaluation and participatory variety selection of improved highland sorghum varieties for smallholder farm at west hararghe zone

Participatory variety selection is the most important breeding program which enhanced the adoption of improved varieties through creating awareness based on smallholder farmers’ selection criteria. Several different improved sorghum varieties are released at different international and national research institutions at different times, however, the technologies were not properly addressed the farmers based on participatory, client oriented and demand driven. The experiment was carried out in West Hararghe Zone; Oromia Regional State, Ethiopia with the objective to identify and recommend the best adapted and performed improved sorghum varieties with farmers’ preference traits through continuous performance evaluation at different stages of the sorghum. A total of six sorghum varieties were evaluated in randomized complete block design in the 2019 main cropping season. Farmers have evaluated the entire experimental units using different statistical tools like direct-matrix and pair-wise ranking algorithms at maturity stage. Farmers set selection criteria to identify the superior improved varieties as compared to the local check by listing different agronomic traits like yield, biomass, seed color, seed size and biotic and abiotic resistance. The analysis of variance showed that there was highly significant difference (p<0.01) among the genotypes for all studied traits. The greatest grain yield was recorded from the variety Dibaba (11325 Kgha-1) and Jiru (10200 Kgha-1) respectively. Likewise, based on the overall farmer’s preference, Dibaba and Jiru were ranked first and second and followed by Adelle, ETS2752 and Chiro respectively. Additionally, the study revealed that participatory varietal selection is playing decisive role in gathering farmers’ perceptions, preferences, merits and shortcomings of sorghum varieties for future improvement. Hence, based on the result of the study, variety Dibaba and Jiru were recommended for multiplication and distribution to farmers through both formal and informal seed systems. Generally, the integration of plant breeders and farmers’ perceptions are used to increase the adoption rate and design a good breeding program for future improvement.

Water stress intensified the relation of seed color with lignan content and seed yield components in flax (Linum usitatissimum L.)

This study aimed to investigate the effect of yellow and brown seed coat color of flax on lignan content, seed yield, and yield components under two contrasting environments of non-stress and water stress conditions. The water stress environment intensified the discrimination between the two seed color groups as the yellow seeded families had lower values for seed yield components under the water stress. Heritability and the genetic advance for seed yield were significantly higher in brown-seeded families than those of yellow-seeded ones at water stress conditions. Secoisolariciresinol diglucoside (SDG) as the chief lignan in flaxseed was more abundant in yellow-seeded families under the non-stress environment but under water stress conditions, it increased in brown seeded families and exceeded from yellow ones. Considering that the brown and yellow seed color families were full sibs and shared a similar genetic background but differed in seed color, it is concluded that a considerable interaction exists between the flax seed color and moisture stress concerning its effect on seed yield and yield components and also the seed SDG content. Brown-seeded genotypes are probably preferred for cultivation under water stress conditions for better exploitation of flax agronomic and nutritional potentials.

High-yielding cultivar of spring turnip rapeseed of “000” type Gratsiya

The spring turnip rapeseed cultivar Gratsiya was developed at the Siberian experimental station – a branch of V.S. Pustovoit All-Russian Research Institute of Oil Crops by multiple individual and family selections from high-yielding, yellowseeded forms of the German cultivar Eldorado. In 2020, we isolated elite plant in the selection nursery by the main agronomic valuable traits: early maturity, high productivity, resistance to lodging, yellow seed color, low content of glucosinolates in the seeds and erucic acid in the oil. According to the results of three years competitive trials (2018–2020), 12 promising numbers with a growing season of 66–70 days were evaluated and a promising high-yielding number 31119 – the cultivar of type “000” Gratsiya was isolated. The cultivar of spring turnip rapeseed Gratsiya exceeded the standard cultivar Pobeda in seed yield by 0.21 t/ha and in oil yield by 0.08 t/ha. By the main agronomic traits, the cultivar Gratsiya differs from the standard one in lower content of glucosinolates in seeds (14.6 µmol/g) and erucic acid in oil (0.43 %). Seedlings are green, without wax coating, the plant is semi-dense, medium-branched, plant height of 120– 135 cm. The fruit is a pod, 5–7 cm long. Seeds are yellow, with an addition of brown – up to 2 %. Thousand-seed weight is 2.5–2.8 g. It is moderately drought-resistant, has uniform ripening, is suitable for mechanical harvesting, and is recommended for cultivation throughout the Russian Federation because of its early maturity.

SOME TREES AND FRUIT CHARACTERISTICS OF IMPORTANT STANDARD AND LOCAL POMEGRANATE (Punica granatum L.) CULTIVARS GROWN IN KOCAKOY (DIYARBAKIR) DISTRICT

In this study, it was aimed to determine certain tree and Fruit characteristics of important Standard and local pomegranate cultivars grown in Kocaköy (Diyarbakir) province. In this context, 2 pomegranate species (Hicaz and Zivzik), 5 local pomegranate species (Hınara Tırş, Hınara Zer, Hınara Meğoş, Hınar Şirin and Hınara Sor) and 2 local pomegranate genotypes (Hınar-1 and Hınar2) were studied. In the study, Fruit weights ranged from 129.90 -314.59 g; Fruit heights ranged from 54.78-74.28 mm; Fruit diameters ranged from 62.92-86.43 mm; Fruit Volume strange from 129.20-293.50 ml; Fruit juice amounts ranged from 34.90-90.90 ml; Fruit densities ranged from 1.0-1.23 gr/ml; 100 seed weights ranged from 24.46-37.690 g; calix heights ranged from 9.65-21.00 mm and calix diameters ranged from 16.40-27.60 mm. In addition, Soluble solid contents (SSC) ranged from 14.60-17.29% brix; total acidity ranged from 4.02-24.51%; pH ranged from 2.55-4.15 and shape indices ranged from 0.69-0.94. Moreover, sub skin color, upper skin color, seed hardness, fruit taste, seed color, upper fruit compartment, sub fruit compartment, compartment number, compartment appearance, easiness in separating arils and Fruit pulp weights of the genotypes and cultivars were also determined.

Improving the quality of automated VIS–grading of Scots pine seeds using fuzzy logic algorithm

The automation of Scots pine seeds grading in the visible wavelength region – VIS-grading – is promising for conducting breeding and genetic experiments. This will reduce time costs and increase the accuracy of seed color classification compared to organoleptic techniques. When controlling VIS-grading, it is necessary to accurately detect and process the optical signal reflected from a single seed. The signal is based on the wavelength and amplitude of the optical beam. Earlier, using a spectrometer for Scots pine seeds from a natural stand of the Pavlovsky district of the Voronezh region, Russia, the boundaries of three spectrometric groups were established. In the real VIS-grading process, it is necessary to take into account the probabilistic deviations of random values of wavelengths and amplitudes. Therefore, on the basis of the Mamdani fuzzy logic theory developed an analyzing algorithm for controlling the VIS-grading quality. The algorithm consists of a sequence of logical terms that clearly define the specified VIS-grading seeds spectrometric parameters by a combination of wavelength and amplitude. The efficiency of Scots pine seeds VIS-grading using the algorithm is 98.9%.

Medicago ABI3 Splicing Isoforms Regulate the Expression of Different Gene Clusters to Orchestrate Seed Maturation

Seed maturation comprises important developmental processes, such as seed filling and the acquisition of seed germination capacity, desiccation tolerance, longevity, and dormancy. The molecular regulation of these processes is tightly controlled by the LAFL transcription factors, among which ABSCISIC ACID INSENSITIVE 3 (ABI3) was shown to be involved in most of these seed maturation processes. Here, we studied the ABI3 gene from Medicago truncatula, a model legume plant for seed studies. With the transcriptomes of two loss-of-function Medicago abi3 mutants, we were able to show that many gene classes were impacted by the abi3 mutation at different stages of early, middle, and late seed maturation. We also discovered three MtABI3 expression isoforms, which present contrasting expression patterns during seed development. Moreover, by ectopically expressing these isoforms in Medicago hairy roots generated from the abi3 mutant line background, we showed that each isoform regulated specific gene clusters, suggesting divergent molecular functions. Furthermore, we complemented the Arabidopsis abi3 mutant with each of the three MtABI3 isoforms and concluded that all isoforms were capable of restoring seed viability and desiccation tolerance phenotypes even if not all isoforms complemented the seed color phenotype. Taken together, our results allow a better understanding of the ABI3 network in Medicago during seed development, as well as the discovery of commonly regulated genes from the three MtABI3 isoforms, which can give us new insights into how desiccation tolerance and seed viability are regulated.

Evaluation of Ethiopian chickpea (Cicer arietinum L.) genotypes for frost tolerance

<p>Frost stress is one of the most significant abiotic factors affecting chickpea (Cicer arietinum L.) production in the Ethiopian highlands. To investigate the frost tolerance of chickpea, 673 genotypes were characterized using an augmented design at Bakelo, Debre Berhan, Ethiopia for two years. A significant (p &lt; 0.01) variability amongst genotypes was recorded for all agronomic traits considered. A considerable number of accessions better performing over the frost susceptible genotypes were identified for agronomic traits. Stem/leaf pigmented genotypes showed a better reaction for frost stress than non-pigmented genotypes. The majority of black seeded chickpea adapted well under frost stress when compared to with brown and white seeded genotypes. According to the freezing tolerance rate (FTR) and plant survival rate (SR), 83 (12.3 %) and 85 (12.6 %) genotypes were identified as frost tolerant. There was a strong correlation (p &lt; 0.01) in grain yield with FTR, SR, seed shriveling score, stem/leaf pigmentation and seed color. Based on our findings, Ethiopian chickpea landraces has a good genetic potential for frost resistance traits for use in breeding programs. </p>