CYTOCHROME B BASED GENETIC RELATIONSHIP OF WILD BOARS FROM DAK NONG PROVINCE OF VIETNAM

Nghiên cứu nhằm đánh giá mối quan hệ di truyền của heo rừng tỉnh Đắk Nông dựa trên trình tự gene cytochrome b. Kết quả sắp xếp trình tự cho thấy tồn tại 26 điểm đa hình đơn nucleotide (SNP) giữa các haplotype ở heo rừng Đắk Nông với các haplotype khác từ châu Á và châu Âu. Vùng biến đổi mạnh xuất hiện từ vị trí 15029 đến 15045. Haplotype DKN3 và nhóm heo rừng bản địa Việt Nam biểu hiện một vị trí các SNP giống hệt nhau là TATG; trong khi các haplotype DKN1, DKN4-DKN7 và nhóm heo rừng lai Việt Nam cùng biểu hiện hai vị trí SNP CATA và CATG. Các haplotype DKN1, DKN4-DKN7 và nhóm heo rừng lai Việt Nam có mối quan hệ di truyền chặt chẽ với lợn rừng châu Á; trong khi haplotype DKN3 và heo rừng bản địa Việt Nam nằm trong nhánh tách rời của cây phát sinh loài. Kết quả này cho thấy có hai quần thể heo rừng ở Đắk Nông, dẫn đến kết luận rằng heo rừng bản địa Việt Nam và heo rừng lai Việt Nam có sự phân bố trải rộng khắp Tây Nguyên.

Genetic Distance of Four Strains of Guppy Fish (Poecilia reticulata) Using RAPD PCR Method

Guppies are one type of organism that can live well, especially in the tropics. The kinship between species that have genetic similarities can be identified through genotypic mapping, one of which is molecular analysis using. RAPD method with PCR technique. This study aims to determine the genetic relationship of four guppy poecilia reticulata strains, respectively are Albino Full Red (AFP), Brazilian Fan Tail (BFT), Koi Guppy Tuxedo (KGT), and Platinum Red Tail Big Ears (PRTB) with the RAPD-PCR method. The genetic relationship data obtained is used as a guide for mating between the four strains. This research was conducted from September 2020 to November 2020. The process were carried out at the Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University and the Central Laboratory, Padjadjaran University. OPA-03 primer (AGTCAGCCAC) is used as a standard parameter to interpret genetic diversity among the four guppy strains. Based on the results, amplification with primer OPA-03 visualized 21 bands consisting of seven polymorphic bands and 13 monomorphic bands. The results of the phylogenetic tree showed that there were two groups. The first group is AFR and BFT a similarity index of 69.5%. The second group is KGT and PRTB a similarity index of 71.5%.

Modern Stylization of Ukrainian Song Folklore

The purpose of the research is to study contemporary musical culture and reveal the specifics of Ukrainian song folklore stylization in academic and non-academic practice. The research methodology is aimed at using a comparative method, with the help of which the difference between the processing of a folk song and the stylization of folklore is highlighted. The method of analysis is implemented to highlight the essential features of stylized folklore. Using the synthesis method, the results of research from academic and non-academic musical creativity have been extrapolated, in which the introduction of the stylization of folklore has been discovered. The scientific novelty of the research is examining the symbiosis of composer and folk art, as a result of which stylized song folklore appears in the modern musical culture of Ukraine. Conclusions. In the modern musical culture of Ukraine, the principle of stylization of song folklore is widely used. The introductions of adaptations of folk sources, along with stylization, are two options for the interaction of composer and folk art. The stylization is characterized by a deep interpenetration of elements of the author’s and folklore principles, which is accompanied by the formation of an original musical text that has a genetic relationship with folk Melos. The author’s text, which traces the presence of folk songs stylization, often includes typical intonational complexes with small ambitus, repetition of rhythmic formulas, and the prevalence of the variant-variative principle of the musical fabric development. The literary text of works related to the stylization of folklore is characterized by the use of folk texts or dialectics, lexemes that liken a vocal work with folklore. The effectiveness of folk stylization in musical culture is enhanced by the use of an authentic folk singing manner. The stylization of folklore is inherent both in the works of the leading classics of the Ukrainian composing school and in groups working in different directions of non-academic music of our time.

Unstable Relationship Between Braarudosphaera bigelowii (= Chrysochromulina parkeae) and Its Nitrogen-Fixing Endosymbiont

Marine phytoplankton are major primary producers, and their growth is primarily limited by nitrogen in the oligotrophic ocean environment. The haptophyte Braarudosphaera bigelowii possesses a cyanobacterial endosymbiont (UCYN-A), which plays a major role in nitrogen fixation in the ocean. However, host-symbiont interactions are poorly understood because B. bigelowii was unculturable. In this study, we sequenced the complete genome of the B. bigelowii endosymbiont and showed that it was highly reductive and closely related to UCYN-A2 (an ecotype of UCYN-A). We succeeded in establishing B. bigelowii strains and performed microscopic observations. The detailed observations showed that the cyanobacterial endosymbiont was surrounded by a single host derived membrane and divided synchronously with the host cell division. The transcriptome of B. bigelowii revealed that B. bigelowii lacked the expression of many essential genes associated with the uptake of most nitrogen compounds, except ammonia. During cultivation, some of the strains completely lost the endosymbiont. Moreover, we did not find any evidence of endosymbiotic gene transfer from the endosymbiont to the host. These findings illustrate an unstable morphological, metabolic, and genetic relationship between B. bigelowii and its endosymbiont.

KHATTIC AND ABKHAZO-ADYGH TOPONYMS: A COMPARATIVE HISTORICAL INTERPRETATION

On the basis of a comparison of the Hattian and AbkhazAdyghe toponyms, an attempt is made to confirm the ethnic and genetic relationship of the ancient Hattian and modern AbkhazAdyghe languages, which have no written history. Therefore, toponyms, as material for historical and ethnographic research, are of lasting importance. The Hattian stems are given in the Latin alphabet, their Abkhaz-Adyghe counterparts are in the Cyrillic alphabet. Analysis and comparison of the Hattian and Abkhaz-Adyghe components of toponyms made it possible to reveal their coincidence in form and meaning and the same archetype. Thus, despite the long period of time separating these languages, the correspondences in the structure of toponyms allow us to speak about their relationship and habitat of the Abkhaz-lingual and Adyghe-lingual ethnic groups, as well as the ways of their migration. The research results can be used in writing the history of the Hattian and Abkhaz-Adyghe languages and in the reconstruction of languages that do not have a written language.

The phenotypic of durian plant (Durio zibethinus Murr.) in Tapanuli Tengah Regency, North Sumatra, Indonesia

Durian is a plant that is prone to genetic erosion. Exploration and characterization must be carried out to protect the local durian germplasm in Tapanuli Tengah Regency. This study aims to characterize the morphology of durian plants and determine the genetic relationship between the accessions of local durians in Tapanuli Tengah Regency. The research was done at Tukka, Sitahuis, and Kolang districts from December 2018 to February 2019 with a survey method using durian descriptor of IPGRI. Sampling techniques using purposive sampling with location judgment and morphological observation data were processed using NTSYS-pc 2.02 software and genetic relationship using the IBM SPSS (Statistical Package for the Social Sciences) program version 2.02 with cluster analysis. The result of the research showed that in Tukka, Sitahuis and Kolang district there were 75 accessions of durian been identified. The closest relationship is obtained on A61 and G71 originating from Kolang District with a similarity value of 7,345. The furthest relationship was obtained from A1 and A25 from Tukka district with the similarity value of 94,097.

Amplification rate of matK and rbcL genes in three types of durian

Durian is a tropic fruit having numerous variations on its fruits. Its variations are not only in its shape but also in its aril fruit, aril color, flavor, and aril thickness. In addition to its fruit variations, the genus Durio also has many species which quite hard to distinguish morphologically, except during flowering and fruiting times. This study aimed to determine the genetic relationship among Durian, Pelangi Atuturi Variety Durian, Durio graveolent, and Durio zibetinus based on chloroplast genes (RbcL and matK genes). The primers were previously designed for amplifying matK and rbcL genes based on the Durio zibethinus sequence. Both genes were used because of having great competence to describe genetic relationships between plant species. The rbcL primer could amplify all evaluated samples. Meanwhile, matK primer generated a smeared band in Durian Pelangi; thus, we did not obtain any sequence of this plant. Sequence analysis showed no variation of rbcL sequence in these evaluated species. A similar result was also observed on D. zibethinus and D. graveolent. Overall, both genes could not describe the genetic relationship among the evaluated durians, and they were grouped in the same cluster in phylogenetic.

Understanding the plant-pathogen interaction associated with Septoria nodorum blotch of wheat

This chapter discusses understanding the plant-pathogen interaction associated with septorium nodorum blotch of wheat. It begins by reviewing the necrotrophic effector-host sensitivity gene interactions in the wheat-P. nodorum system. It then reviews the genetic relationship between NE-sensitivity gene interactions and the importance of these interactions in the field. Additional QTL associated with susceptibility/resistance to P. nodorum is also discussed, followed by a section on the impact of genome sequencing in characterizing NE-sensitivity gene interactions.