Manufacture of Banana-Based Paper

The paper is one of the most used elements in modern society, despite the fact that its invention dates back to ancient times. The paper is used for different domestic, labor and industrial purposes. Its manufacture is made from cellulose pulp and vegetable fibers, which have had a significant environmental impact in recent years. This work proposes the analysis of the production of paper from the use of residues of the banana plant, which could offer a better use of natural resources and a lower impact on nature. In addition, the natural fiber of bananas is resistant and is easily obtained in Latin American countries such as Ecuador, which has a high production of it. The results found show that it is possible to make paper with fiber from banana residues and that it is possible to commercialize and make good use of resources. Keywords: Organic paper, banana waste, environmental protection. References [1]A. Herrera, «ELABORACIÓN DEL PAPEL ORGÁNICO A BASE DE PSEUDOTALLO DE BANANO, CANTÓN SANTA ROSA, PROVINCIA DE EL ORO, ECUADOR,» Guayaquil, 2019. [2]R. d. León, «Fabrican papel con desechos de plátano,» CIENCIAMX, 1 julio 2016. [En línea]. Available: http://www.cienciamx.com/index.php/tecnologia/biotecnologia/8476-generacion-de-celulosa-a-partir-de-desechos-de-la-planta-del-platano. [3]A. Cortéz, «ELABORACIÓN DE PAPEL A BASE DE RESIDUOS DE BANANO,» Guayaquil, 2014. [4]M. Dávalos and S. Zurita, «Organic paper, banana waste, environmental protection,» Universidad Politécnica del Litoral, Chile, 2004. [5]G. Castillo-Tumaille and W. Espinoza- Espinoza, «Análisis de aceptación del papel a base de pinzote de banano como alternativa de,» Ingeniería Industrial. Actualidad y Nuevas Tendencias, vol. V, nº 18, pp. 59-70, 2017. [6]M. Mazzeo, L. León, L. Mejía, L. Guerrero and J. Botero, «APROVECHAMIENTO INDUSTRIAL DE RESIDUOS DE COSECHA Y POSCOSECHA DEL PLÁTANO EN EL DEPARTAMENTO DE CALDAS,» Educación en Ingeniería, vol. junio de 2010, nº 9, pp. 128-139, 2010. [7]M. Arzola and A. Mejías, «Modelo conceptual para gestionar la innovación en las empresas del sector servicios,» Revista Venezolana de Gerencia, vol. 12, nº 37, pp. 80-98, 2007. [8]I. Azman and S. Yusrizal, «Service quality as a predictor of satisfaction and customer loyalty.,» Scientific Journal of Logistics. , vol. 12, nº 4, pp. 269-283, 2016.

Formulasi Sediaan Krim Dari Ekstrak Kulit Pisang Kapas (Musa paradisiaca Linn).

Musa paradisiaca Linn, also known as the banana plant in Indonesia, is a herbaceous plant that belongs to the Musaceaa family. Cotton banana peel (Musa paradisiaca Linn.) can be used as a wound medicine. For use, it is still simple, namely by applying the inside of the banana peel to the injured body part, and by scraping the banana peel from the inside to bandage the wound. For this reason, it is necessary to develop a dosage form of this cotton banana peel so that it is easier and provides comfort in its use. Purpose: To find out the skin of cotton banana (Musa paradisiaca Linn) is formulated in cream preparations. The method in this study was experimental, the sample was extracted by maceration using 96% ethanol as solvent. The viscous extract obtained was used at concentrations of 0.08%, 0.10%, and 0.12%. The results showed that cotton banana peel extract (Musa paradisiaca Linn) could be made as a cream and fulfilled the physical evaluation of the preparation. The results of the homogeneity test were that the preparations were made homogeneous, the pH of the cream was obtained at pH 5-7 still fulfilling the skin pH range of 4-7, the adhesion test results obtained more than 5 seconds, the dispersion test results obtained 6-7 cm, the viscosity test at formula I is 3564 – 4253 cPas, Formula II is 4042 – 4746 cPas, Formula III is 4466 – 5254 cPas. The conclusion of this study, the formulation of the ethanol extract cream of cotton banana peel (Musa paradisiaca Linn) met the requirements for physical evaluation of the preparation including homogeneity test, pH test, organoleptic test, dispersibility test, adhesion test, viscosity test and stability test.Keywords: Formulation, banana peel, cream, evaluation. AbstrakMusa paradisiaca Linn atau dikenal dengan nama tumbuhan pisang di Indonesia adalah tumbuhan herba yang termasuk dalam keluarga Musaceaa. Kulit buah pisang kapas (Musa paradisiaca Linn.) dapat digunakan sebagai obat luka. Untuk penggunaan masih sederhana yaitu dengan mengoleskan bagian dalam dari kulit buah pisang pada bagian tubuh yang luka, dan dengan cara mengorek kulit buah pisang dari dalam untuk membalut luka. Untuk itu perlu adanya pengembangan bentuk sediaan dari kulit pisang kapas ini sehingga lebih memudahkan dan memberikan kenyamanan dalam penggunaannya. Tujuan Untuk mengetahui kulit buah pisang kapas (Musa paradisiaca Linn) diformulasikan dalam sediaan krim. Metode pada penelitian ini dilakukan secara eksprimental, sampel di ekstraksi dengan cara maserasi dengan menggunakan pelarut etanol 96%. Ekstrak kental yang diperoleh digunakan pada konsentrasi 0.08%, 0.10%, dan 0.12%. Hasil penelitian menunjukkan bahwa ekstrak kulit pisang kapas (Musa paradisiaca Linn) dapat dibuat sebagai krim dan memenuhi evaluasi fisik sediaan. Hasil uji homogenitas bahwa sediaan yang dibuat homogen, pH krim diperoleh pH 5-7 masih memenuhi kisaran pH kulit 4-7, uji daya lekat hasil yang diperoleh lebih dari 5 detik, uji daya sebar hasil yang diperoleh 6 – 7 cm, uji viskositas pada formula I yaitu 3564 – 4253 cPas, Formula II yaitu 4042 – 4746 cPas, Formula III yaitu 4466 – 5254 cPas. Kesimpulan pada penelitian ini, formulasi sediaan krim ekstrak etanol kulit pisang kapas (Musa paradisiaca Linn) memenuhi syarat evaluasi fisik sediaan meliputi uji homogenitas , uji pH, uji organoleptis, uji daya sebar, uji daya lekat, uji viskositas dan uji stabilias.Kata kunci: Formulasi, kulit pisang, krim, evaluasi.

Comparative Study on Treatment using Water-based Extract of Fresh and Dry Unripe Banana Peels with Conventional Antibiotics on UTI

Almost every part of a banana plant has use in medicine. Increased bacterial resistance to the conventional antibiotics has led to research into the development of alternative treatments to conventional antibiotics. This study focuses on the antimicrobial properties of banana peels against selected isolates from Urinary Tract Infection sample. The isolates from cultures further analysed with agarose gel electrophoresis for the presence of 16SrRNA and Phylogenetic analysis revealed Staphylococcus sciuri strain, a coagulase‐negative species, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis. Fresh unripe and dry unripe were used to determine the antimicrobial activities. A measure of 150grams of both fresh unripe and dry unripe was extracted with 100ml of water solvent (sterile) respectively for about 2days. The solvent extracts were concentrated separately under reduced pressure, 10g of each concentrated solvent extracts were dissolved in 5ml of sterile distilled water and used for antimicrobial assay using agar well diffusion method. The phytochemical analysis of fresh unripe and dry unripe revealed that alkaloid, flavonoid, cardiac glycosids,and phenols were present.. Musa sapientum L peels showed some effect on Escherichia coli, Enterococcusfaecalis, Staphylococcus sciuri, Klebsiellapneumoniae and Proteus mirabilisat 80% concentrations for water. Dry unripe has advantage over fresh unripe banana peels. The peels of Musa sapientum exhibited some inhibitory activity on these selected UTIs isolates, which could be attributed to the presence of certain secondary metabolites. Finally, when compared the peels extracts against the standard antibiotics drugs as the control, the water solvent extract were less effective.

Growth and nitrogen accumulation assessment of banana (Musa acuminata) after exposure to hydroquinone in a charcoal-ultisol mix

The present study investigates plant growth and nitrogen accumulation assessment of young banana plant (Musa acuminata) after exposure to hydroquinone (a mutagen) in a charcoal-ultisol mix. Charcoal was obtained from the wood of Pentaclethra macrophylla aerobically, and was crushed into a smooth powdered form. This was mixed with an ultisol obtained from the University of Benin Botanic garden. Different levels of the soil-charcoal mix were prepared as follows; 100% charcoal, 75% charcoal-25% soil, 50% charcoal-50% soil, 25% charcoal- 75% soil, and 100% sand. The charcoal-ultisol mix was then amended with 5ppm hydroquinone one week before propagating young banana suckers. Results showed that sprouting began fifteen days after propagation. There were also different changes in plant height in the various soil treatments; the lowest was reported in the 75-25 charcoal ultisol mix (10.50cm), compared to 45.83cm in plants sown in 100% charcoals. Foliar yield was lowest in the 75-25 charcoal-ultisol mix (8.55 grams per plant), compared to 29.15 grams per plant in the 100% charcoal. The below ground morphological characteristics test plant revealed that there were significant differences between the growth parameters. Nitrate nitrogen, Ammonia Nitrogen and total nitrogen were significantly accumulated on the leaves of plant. However, higher nitrogen accumulation in the leaves was found in treatment with high charcoal percentage.

Characterization and virulence of Fusarium oxysporum f. sp. cubense cause wilt disease in banana plants and its biological control using endophytic fungi Trichoderma spp. at West Nusa Tenggara, Indonesia

One of the obstacles in developing banana plants is the presence of Fusarium wilt disease caused by the fungus Fusarium oxysporum f. sp cubense. This fungus is difficult to control because it has a survival structure in the form of chlamydospores that can survive as a saprophyte. One way to control it is biologically using the antagonist endophytic fungi Trichoderma spp. This study aimed to determine the characteristics and virulence of the fungus F. oxysporum f. sp cubense and its control using the endophytic fungi Trichoderma spp. The study used an exploratory method carried out in an endemic area of Fusarium wilt disease to isolate the fungus F. oxysporum f. sp cubense and the endophytic fungi Trichoderma spp. in the banana plant tissue. The research was continued in the laboratory, in the greenhouse, and in the field to test the effectiveness of the fungi Trichoderma spp. The results showed that seven isolates of the fungus F. oxysporum f sp cubense with different characteristics. Six isolates of the endophytic fungi Trichoderma spp. which is effective in suppressing the growth of the fungus F. oxysporum f. sp cubense, namely T. harzianum, T. koningii, T. aureoviride, T. hamatum, T. viride, and T. piluliferum.

Microbial Retting of Banana Pseudostem

Fibrous wastes correspond to approximately 54.3 wt.-% of a banana plant. The waste weight is mainly of bunches, stems, and leaves. These ligno-cellulosic fibre waste can be designed and tailored to yield value added products which can be exploited by the farmers, exporters and weavers. Pectin (plant cement) and hemicelluloses (22-25%) found in plant cell walls in the plate (middle lamella). These macromolecules have needs to be hydrolyzed faster without affecting the quality of fibre. The chemical and mechanical processes adopted for fibres actually affect color uptake, strength of fibre thus fetching lower price. We report a biological ecofriendly method of retting where pectinases produced by the endophytic microorganisms of banana pseudostem were used to separate the fibre bundles of cellulose.. Endophytes are microorganisms which live in close relationship with living plant tissues in a cooperative relationship delivering a few auxiliary metabolites and proteins with the possibility to hydrolyse a few plant-determined macromolecules of the host. They spend the entire or part of their lifecycle colonizing within sound tissues of the host plants.The endophytic pectinase helps to break down the cell walls and can find commercial application in extraction of fibres.Seven bacterial isolates were screened and isolated in pectinase screening agar medium [PSAM]. Pectinase producing endophytic bacteria is isolated from corm of Amorphophallus paeoniifolius reported by us are Staphylococcus sciuri, Exiguobacterium acetylicum, Exiguobacterium indicum which are good pectinase producers having specific activity of 8.26, 12.61, 6.81 respectively. Treatment of banana pseudostem with these microbes showed 91%, 94%, 96.7% loss in the total weight of stem leaving behind pure cellulose fibres.

Single Stem Rouging of Banana Xanthomonas Wilt Infected Plants for Orchard Rehabilitation

Banana Xanthomonas Wilt (BXW), caused by Xanthomonas campestris pv. musacearum (Xcm), a devastating disease, causes up to 100% loss and affects all banana varieties. The disease is widespread in East and Central Africa region. Study objective was to evaluate the performance of single stem rouging options for rehabilitation of severely affected banana orchard. Five single stem rouging options were implemented in banana orchard with 80% BXW severity in Western Kenya. The options included rouging of infected banana plants; uprooting, cutting at the base, injecting 20 ml of glyphosate herbicide at the soil base of the pseudostem, uprooting the whole stool and control leaving the infected stools. Initial visual assesment before implementation of the orchard was over 80% infection. Later samples were collected for diagnosis of ten plants per treatment from different plant parts and were subjected to ELISA and PCR procedures for confirmion of the presence of Xcm. Rouging options was implemented for one year. During and after experimentation periodic visual assesment of BXW incidence and severity reduced. Results of ELISA assays and PCR results indicated Xanthomonas was present on banana plants. Rouging the infected banana stems by cutting at the base, uprooting and injection of herbicide reduced BXW incidence within six months. Xanthomonas, an inhibiting bacteria cannot surve without host and by killing the banana plant the Xanthomonas dies. Yields were recovered from the rehabilitated orchard within one year. Banana orchard highly infected with BXW was effectively rehabilitated.

Analysis of the Values of Enset (Enseteventricosum) in The Socio-Cultural Life of Thegurage of South-Central Ethiopia

Enset (Enseteventricosum) is a traditional multi-purpose crop mainly used as a staple/co-staple food crop over 20 million people in Ethiopia. The Gurage are sedentary agricultural people of patrilineal persuasion who speak a Semetic language and inhabit in a sparsely fertile semi-mountainous regionin south-central Ethiopia. Enset, their staple food crop, commonly called the “false banana plant”, is produced in abundance by each Gurage homestead. The objective of this study was to document the socio-cultural values of enset plant among the Gurage. In this study, a qualitative methodological approach is employed in extracting information from different sources on the subject in question. The study relied mainly on primary and secondary sources.According to findings, three types of food, viz, Kocho(fermented product from scraped pseudo stem grafted corm), Bulla(dehydrated juice), and Amicho (boiled corm) can be prepared from enset. As a food crop, it has useful attributes such as foods can be stored for long time, grow in wide range of environments, produces high yield per unit area and tolerates drought. It has irreplaceable role as a feed for animals. Enset starch is found to have higher and widely used as a tablet binder and dis-integrant and also in pharmaceutical gelling, drug loading and release processes. Moreover, enset shows high genetic diversity within a population which in turn renders resilience and food security against the ever-changing environmental factors and land use dynamics.Enset is totally involved in every aspects of the daily social and ritual life of the Gurage, who, with other several tribes in southwest Ethiopia, form what has been termed “the EnsetCulture Complex Area”. From birth, when the umbilicus is tied off with a fiber drawn from enset fronds, the life of the Gurage is enmeshed with various uses of enset, not the least of which is nutritional.