In present work, multi-metallic nanoparticles were synthesized by chemical method in a controlled
environment by using silver, lead, mercury, egg shell powder (contains 1% calcium phosphate, 1%
magnesium carbonate, 94% calcium carbonate and 4% organic matter), potassium nitrate, potassium
alum and extracts of citrus lemon by following the process defined in Traditional Indian Medicine,
Siddha System of Medicine. The morphology, compositions and structure of the product were
characterized by powder X-ray diffraction (PXRD), Fourier transform infrared (FTIR), scanning electron
microscopy (SEM), energy dispersive X-ray (EDX) and transmission electron microscopy (TEM)
techniques. Highly uniform spherical multi-metallic nanoparticle was subjected for the antibacterial
activities. The particles were agglomerated as observed by SEM micrographs. The particles were
homogeneous, spherical in shape and loosely agglomerated as seen by TEM pictures. The antibacterial
activity of the synthesized multi-metallic nanoparticles against B. cereus, S. aureus, E. coli and P.
aeuroginosa was demonstrated using the zone of inhibition technique. The synthesized multi-metallic
nanoparticle can find plausible biological applications.