Biochemical Characterization, Antifungal Activity, and Relative Gene Expression of Two Mentha Essential Oils Controlling Fusarium oxysporum, the Causal Agent of Lycopersicon esculentum Root Rot

Tomato (Lycopersicon esculentum Mill.) is important food in daily human diets. Root rot disease by Fusarium oxysporum caused huge losses in tomato quality and yield annually. The extensive use of synthetic and chemical fungicides has environmental risks and health problems. Recent studies have pointed out the use of medicinal plant essential oils (EOs) and extracts for controlling fungal diseases. In the current research, Mentha spicata and Mentha longifolia EOs were used in different concentrations to control F. oxysporum. Many active compounds are present in these two EOs such as: thymol, adapic acid, menthol and menthyl acetate. These compounds possess antifungal effect through malformation and degradation of the fungal cell wall. The relative expression levels of distinctly upregulated defense-related WRKY genes (WRKY1, WRKY4, WRKY33 and WRKY53) in seedling root were evaluated as a plant-specific transcription factor (TF) group in different response pathways of abiotic stress. Results showed significant expression levels of WRKY, WRKY53, WRKY33, WRKY1 and WRKY4 genes. An upregulation was observed in defense-related genes such as chitinase and defensin in roots by application EOs under pathogen condition. In conclusion, M. spicata and M. longifolia EOs can be used effectively to control this plant pathogen as sustainable and eco-friendly botanical fungicides.

Design of an Artificial Intelligence of Things Based Indoor Planting Model for Mentha Spicata

In recent years, many large-scale plantings have become refined small-scale or home plantings. The rapid progress of agriculture technologies and information techniques enables people to control the growth of agricultural products well. Hence, this study proposes an Artificial Intelligence of Things (AIoT) based Plant Pot Design for planting edible mint in an office setting, which is called APPD. APPD is composed of intelligent gardens and a cloud-based service platform. An intelligent garden is deployed an Arduino with multiple sensors to monitor and control plant pots of the edible mint, Mentha spicata. The cloud-based service platform provides a Case-Based Reasoning (CBR) inference engine with a database for adjustment influence factors. This study discusses eight growing statuses of Mentha spicata with different illumination, photometric exposure, and moisture content, designed for an office environment. Evaluation results indicate that Mentha spicata with 16 h red–blue lighting and 50% moisture content makes a maximum 5% mint extract of the total weight of the mint leaves. Finally, APPD can be a reference model for researchers and engineers.

An ethno-botanical study of indigenous medicinal plants and their usage in rural valleys of Swabi and Hazara region of Pakistan

An ethnobotanical study was conducted to document indigenous medicinal plants and their usage from knowledgeable and elderly persons in Razzar and Gadoon valley of Swabi and Allai and Tanawal valley of Hazara region of Pakistan during 2016-2019. Several systematic field visits and questionnaire surveys were carried out in selected sites of the study area to gather relevant information from the local community. Rapid assessment method was adopted for data collection by interviewing the local people having enough knowledge of medicinal plants use for treatment of different ailments. UV (UV) formula was applied to calculate the relative importance of medicinal plant species in each site of the study area. In the present study, 221 medicinal plants belonging to 105 families have been reported through 580 respondents (385 males, 138 females and 57 local health healer) from the Swabi and Hazara region. The main sources of herbal medicines were leaves (21%) followed by fruits (21%), seeds (17%), whole plants (14%), roots (9%), bark (9%), flowers (7%) and gum (2%). Mentha spicata L. and Berberis lycium Royle were reported with highest UV (UV) i.e. 0.92 and 0.68 in Razzar tehsil and Gadoon valley of Swabi, whereas Mentha longifolia L and Geranium wallichianum D were reported with highest UV i.e. (0.65) and (0.88) in Allai and Tanawal valley of Hazara region, respectively. It was concluded that Swabi and Hazara region is rich in medicinal plants species and associated traditional knowledge. Moreover, ethno-medicines have played significant role in the indigenous healthcare system of the study area. However, uprooting the entire plant for ethno-medicine is a big threat to conservation of medicinal plants diversity in the study area.

Spearmint (Mentha spicata) Cultivation in Decoupled Aquaponics with Three Hydro-Components (Grow Pipes, Raft, Gravel) and African Catfish (Clarias gariepinus) Production in Northern Germany

Mint (Mentha spicata) was cultivated in different hydroponic components: grow pipes, a raft and an ebb-and-flood gravel substrate system irrigated with aquaculture effluents from intensive African catfish (Clarias gariepinus) production under decoupled aquaponic conditions in northern Germany. The spearmint grew well and plant heights above ground were not significantly different between the gravel (57.7 ± 13.1 cm), raft (58.0 ± 17.7 cm) and grow pipe components (63.6 ± 9.9 cm). Root lengths and root fresh weights were two-fold and four-fold higher in raft (64.3 ± 20.5 cm; 42.8 ± 29.9 g) and grow pipes (59.4 ± 15.2 cm; 41.3 ± 25.7 g) compared with gravel substrate (29.7 ± 7.8 cm; 9.4 ± 9.4 g; raft = grow pipes > gravel). Spearmint leaf number was significantly higher in the grow pipes (770.0 ± 224.4) than in the gravel substrate (499.8 ± 228.4) with intermediate values in the raft. Significantly highest mean fresh biomass was found in the raft (1275.6 ± 33.4 g), followed by grow pipes (1042.0 ± 35.8 g) and gravel substrate (686.3 ± 98.2 g; raft > grow pipes > gravel). M. spicata, under aquaponics, grows best in grow pipe and raft components. An increase in pipe diameter for the grow pipes and a reduction in the channel height for the raft components could optimize aquaponic culture conditions for both industrial production and the hobby sector in the future.

Boosted Antioxidant Effect Using a Combinatory Approach with Essential Oils from Origanum compactum, Origanum majorana, Thymus serpyllum, Mentha spicata, Myrtus communis, and Artemisia herba-alba: Mixture Design Optimization

Several studies have demonstrated the possible synergistic effect as an effective strategy to boost the bioactivity of essential oils. Using this framework, this study was conducted to effectively establish the ideal combination of six essential oils from different plants (Origanum compactum, Origanum majorana, Thymus serpyllum, Mentha spicata, Myrtus communis, and Artemisia herba-alba) that would express the best antioxidant activity. Each mixture was optimized using a mixture design approach to generate the most effective blend. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging method was used as a reference method to assess the antioxidant activity. Each essential oil’s composition was identified using the GC/MS method. The single essential oil activities demonstrated variable antioxidant effects, and following the mixture design approach, the optimal antioxidant blend was revealed, as two mixtures demonstrated the best antiradical activity with 79.46% obtained with the mixture of O. majorana (28%) and M. spicata (71%) and 78.8% obtained with the mixture O. compactum (64%), O. majorana (13%), and T. serpyllum (21%). This study proposes a practical way to elaborate mixtures in the search for a boosting effect that can be oriented for the food or pharmaceutical industry.

Diploid mint (M. longifolia) can produce spearmint type oil with a high yield potential

Mint oil is a key source of natural flavors with wide industrial applications. Two unbalanced polyploid cultivars named Native (Mentha Spicata L) and Scotch (M. × gracilis Sole) are the main producers of spearmint type oil, which is characterized by high levels of the monoterpenes (−)-carvone and (−)-limonene. These cultivars have been the backbone of spearmint oil production for decades, while breeding and improvement remained largely unexplored, in part, due to sterility in cultivated lines. Here we show that sexual breeding at the diploid level can be leveraged to develop new varieties that produce spearmint type oil, along with the improvement of other important traits. Using field trials and GC-FID oil analysis we characterized plant materials from a public germplasm repository and identified a diploid accession that exhibited 89.5% increase in oil yield, compared to the industry standard, and another that produces spearmint type oil. Spearmint-type oil was present at high frequency in a segregating F2 population (32/160) produced from these two accessions. Field-testing of ten of these F2 lines showed segregation for oil yield and confirmed the production of spearmint-type oil profiles. Two of these lines combined high yield and spearmint-type oil with acceptable analytic and sensory profiles. These results demonstrate that spearmint-type oil can be produced in a diploid background with high yield potential, providing a simpler genetic system for the development of improved spearmint varieties.

A review on the efficacy of Ocimum gratissimum, Mentha spicata, and Moringa oleifera leaf extracts in repelling mosquito

@@@In recent times, repellents and synthetic drugs have been identified as having negative toxicity effects on humans and the environment. Apart from the unfavourable effects on man and livestock caused by these chemicals-based (synthetic) repellents, they are also expensive, non-biodegradable, and no more effective because mosquitoes are getting adapted. With these drawbacks, an eco-friendly plant-based insecticide as a substitute is needed urgently. This paper reviews the extraction and use of essential oil from the leaves of Mentha spicata, Ocimum gratissimum, and Moringa oleifera as mosquito repellent. Carvone, Eugenol, and 9-Octadecenoic acid were discovered to be the most active components in the M. spicata, O. gratissimum, and M. oleifera extracts, respectively, using gas chromatography-mass spectrometry (GC-MS). Highlights In recent times, repellents and synthetic drugs have been identified as having negative toxicity effects on humans and the environment. Apart from the unfavorable effects on man and livestock caused by these chemical-based (synthetic) repellents, they are also expensive, non-biodegradable, and no more effective because mosquitoes are getting adapted. An eco-friendly plant-based insecticide as a substitute is needed urgently. Diseases transmitted by mosquitoes are still a significant reason for the global mortality rate, with over 700 million individuals experiencing such diseases every year. With the proper formulation of other repellent forms using their oils, they can replace non-degradable synthetic mosquito repellents since they are eco-friendly. In general, the mosquitocidal activity and percentage protection of plant extract increase with increasing concentration of the extracts in different formulations. This paper is our original work. We certify that this manuscript has not been published in part or whole elsewhere in any language, and it has not been submitted to any other journal for reviews.