CLINICAL, FAEMATOLOGICAL AND BIOCHEI‘./ECAL STUDIES ON ANAPLASMOSIS IN LOCAL BUFETALOES IN MOSUL, AREA

Fourty five local buffaloes naturally infected with A. marginal age of 2-5 years. Clinical signs, present parasireamia haematological and biochemical changes were studied. Thin thick blood smears Stained with Gimsas stain were used to identify the parasite. The results indicated that most of the infected were suffering from mild form of infection and the 1I11p01”l11I1E' clinical signs observed were mild rise of body temperature, increase respiratory and plus rate, weal: ruminal contraction and generalized weakness. Palcness and iteration of mucous membrane, loss appetite, nasal and ocular discharge, coughing, salivation, atarcp, constipation, diarrhea, and rccumbancy. Statistical analysis showc increase (P<0.01) in body temperature, respiratory and pulsera wlflc nigrrificant decrease (P<0.01) in ruminal contraction We. observed. Parasiteamia in infected animals ranged from 1.3- 5.2%. Values of red blood cell, haemoglobin and packed cell volume were significantly low (P<0.01). Total leulcocyric and neutrophils count was significantly increase (P<0.0l). and (P<0.05) respectively. Blood indices values showed a significant increase in (MCHC) (P<0.0l) while (MCV) values showed no difference, reticulocytes were also present mildly biochemical changes indicated higher levels of AST. Total bilirubin (P<0.01). Total protein concentration was less (P<0.01) and no difference was observed inALT and blood urea nitrogen levels between infected and control animals.

The Effect of Extreme Cold on Complete Blood Count and Biochemical Indicators: A Case Study

Regular exposure to a cold factor—cold water swimming or ice swimming and cold air—results in an increased tolerance to cold due to numerous adaptive mechanisms in humans. Due to the lack of scientific reports on the effects of extremely low outdoor temperatures on the functioning of the human circulatory system, the aim of this study was to evaluate complete blood count and biochemical blood indices in multiple Guinness world record holder Valerjan Romanovski, who was exposed to extremely cold environment from −5 °C to −37 °C for 50 days in Rovaniemi (a city in northern Finland). Valerjan Romanovski proved that humans can function in extremely cold temperatures. Blood from the subject was collected before and after the expedition. The subject was found to have abnormalities for the following blood indices: testosterone increases by 60.14%, RBC decreases by 4.01%, HGB decreases by 3.47%, WBC decreases by 21.53%, neutrocytes decrease by 17.31%, PDW increases by 5.31%, AspAT increases by 52.81%, AlAT increase by 68.75%, CK increases by 8.61%, total cholesterol decreases by 5.88%, HDL increases by 28.18%. Percentage changes in other complete blood count and biochemical indices were within standard limits. Long-term exposure of the subject (50 days) to extreme cold stress had no noticeable negative effect on daily functioning.

Dynamics of Morphological Indicators of Blood of Piglets under the Influence of Iron Clathrochelate Complex and Cyanocobalamin

Iron deficiency anemia is one of the most common non-contagious diseases of piglets. Veterinary antianemic drugs have several drawbacks, so finding new medicines is an important current task for scientists. Therefore, the present study investigated the antianemic effect of iron (IV) clathrochelate in the organism of piglets. The subsequent studies included the exploration of its antianemic actions, particularly in combination with cyanocobalamin when this combination was administered to sows for prophylaxis in piglets. The experiment was carried out on 30 suckling piglets during the period of their detention with sows. According to the method of analogue groups, two groups of control (I) and experimental (II, each containing 15 animals) were formed and they were studied for 30 days. The piglets from five sows (three from each) were selected for the experimental group. During the pregnancy of these sows, 10 ml of 10% solution of iron (IV) IV clathrochelate and solution of cyanocobalamin were injected intramuscularly twice 7 and 14 days before their expected farrowing. For prevention of iron deficiency anemia, the traditional solution of iron dextran was administered once intramuscularly to piglets of the control group. The investigative material included the blood samples of piglets considering the dynamics of probable changes in the number of erythrocytes, hemoglobin content and hematocrit, and other morphological indicators and blood indices of piglets. The dynamics of changes in erythrocyte count, hemoglobin content, hematocrit, leukocytes and platelets, indices of blood almost did not differ from the dynamics of these values when using only 10% solution of iron (IV) clathrochelate for pregnant sows. The proposed scheme for the prophylaxis of iron deficiency anemia in piglets, involving simultaneous intramuscular injections of IV clathrochelate and cyanocobalamin to pregnant sows, is somewhat inferior to the previous preventive measures, which included only the intramuscular injections of iron (IV) IV clathrochelate, but it can be recommended as highly effective.

The importance of aerobic capacity and nutrition in recreational master mountain runners’ performance and race-induced changes in body composition and biochemical blood indices

This study aimed to explore the importance of aerobic capacity and nutrition on 28-km mountain running performance and race-induced changes in body composition and biochemical blood indices, in recreational master athletes of different performance levels. Twenty male master runners (age: 44.6 ± 7.7 years) were divided into two groups, slower and faster runners, according to their race performance. Maximum oxygen uptake [Formula: see text], velocity at [Formula: see text] [Formula: see text], oxygen consumption [Formula: see text], and velocity [Formula: see text] during the second ventilatory threshold were evaluated. Nutrition was assessed for one week before and during the race. Body composition was evaluated, and blood samples were collected before and 3 h after the race. Slower runners exhibited a greater reduction in lean body mass and greater changes in all muscle damage/inflammation/metabolism blood indices than the faster runners ( η2 = 0.201–0.927; p < 0.05). When all the participants were assessed as one group ( n = 20), significant correlations were found between [Formula: see text], vVO2Thr, race time, energy intake, expenditure, carbohydrate, protein intakes, and post-race changes in body composition and blood markers ( r: −0.825–0.824; p < 0.05). The strongest determinants were [Formula: see text] and vVO2Thr, while energy intake, expenditure, carbohydrate, and protein intakes seemed to be the weakest determinants of race performance and race-induced changes in body composition and blood indices. The results suggest that race-induced changes in body composition and blood indices are determined mainly by master runners’ race performance and endurance capacity and to a lesser extent by nutrition. However, it seems that carbohydrate and protein intakes have equal importance.

Blood indices and circulating tumor cells for predicting metastasis of hepatocellular carcinoma after liver transplantation

Objectives Liver transplantation (LT) can benefit the long-term survival of hepatocellular carcinoma (HCC) patients. We hypothesized that circulating tumor cell (CTC) levels and subtypes are intimately associated with metastasis status of HCC patients. This study was designed to test that compositive hematological indices including CTC can provide a prediction of post-LT metastasis. Methods Between 2017 and 2018, 37 HCC patients within Hangzhou criteria receiving LT were included for analysis. The 24-month follow-up was mainly conducted by outpatient and telephone. Blood samples were collected, and hematological indices were examined. The outcomes such as PFS, recurrence, metastasis, location of recurrence/metastasis, and number of metastases were recorded. Results The follow-up analysis showed that microvascular invasion (MVI) classification at the baseline is associated with metastasis. Next, α-fetoprotein (AFP) level was another useful indicator of postoperative metastasis, especially at the third or fourth month; the protein induced by vitamin K absence or antagonist-II (PIVKA-II) level three months after LT was significantly higher for those who had later metastasis. The mesenchymal CTC level at the 45th day was increased for in the metastasis group. Using two-ends Logistic regression, the calculated value MP (metastasis predictor, by above factors). Had an AUC of 0.858 in the ROC curve, with a cutoff value of 0.328. Conclusions In conclusion, microvascular invasion, AFP level at the third or fourth month, PIVKA-II level at the third month, and mesenchymal CTC level at day 45 were associated with post-LT metastasis. Using Logistic regression based on above variables, the two-year metastasis can be predicted with satisfactory sensitivity and accuracy.

Evaluation of Variations in the Various Blood Indices in COVID-19 Recovered Patients

Introduction: In December 2019, cases of pneumonia with an unknown cause were reported in Wuhan, Hubei Province, China. Novel coronavirus infectious disease (COVID-19) has been spreading worldwide and tracking laboratory indexes during the diagnosis and treatment of patients with severe COVID-19 can provide a reference for patients in other countries and regions. The disease is caused by the Severe Acute Respiratory Syndrome Coronavirus according to studies, and the World Health Organization just dubbed it coronavirus disease 2019. Aim: The aim of this analysis was to evaluate COVID-19 patients' blood parameters changes in comparison with healthy controlled patients. Methods: Blood samples were taken from 10 patients in which 5 are COVID-19 recovered patients and 5 are healthy controls. For these blood samples TBC (Total Blood Count) was taken and the readings of RBC, hemoglobin, WBC, lymphocyte, granulocyte and platelets count was recorded. Independent t-test was done to obtain the results. SPSS software Version 23 was used to give the output comparison as error bar charts. Results: The patients have increased RBC count, increased hemoglobin and reduced WBC count with reduced lymphocytes and Granulocytes counts. Here it can be concluded that COVID-19 recovered patients should take care of themselves by having proper care, doctor consultation and follow up. Conclusion: From this study it can be understood that COVID-19 recovered patients have increased RBC count and hemoglobin percentage. The recovered patients have reduced WBC, lymphocytes and Granulocytes percentage.

INFLUENCE OF DIFFERENT DOSES OF TISSUE BIOSTIMULANT ON BIOCHEMICAL BLOOD INDICES OF YOUNG CATTLE

Изучено влияние введения разных доз тканевого биостимулятора на биохимические показатели сыворотки крови ремонтного молодняка крупного рогатого скота. Опыт проводился в Алтайском крае на 4 группах ремонтных телочек Приобского типа черно-пестрой породы по 10 голов в каждой. При подборе животных учитывались возраст (1 мес) и живая масса (51,3±1,48 кг). Продолжительность опыта составляла 14 дней. Животным контрольной группы подкожно однократно вводили физиологический раствор в дозе 3,0 мл на 1 голову, I опытной — тканевый биостимулятор в дозе 2,0 мл, II — 3,0 мл, III — 4,0 мл на 1 голову. Опытную партию тканевого биостимулятора изготовили из субпродуктов и боенских отходов пантовых оленей по запатентованной технологии. Материалом для приготовления препарата служили мезентеральные лимфоузлы и средостения, селезенка, печень, матки с плодами (2—3 мес), плацента, отобранные в асептических условиях во время убоя здоровых животных. Введение тканевого биостимулятора телочкам в разных дозах способствовало повышению некоторых исследуемых биохимических показателей сыворотки крови. Наиболее оптимальной дозой применения тканевого биостимулятора следует считать 3,0 мл/гол, что способствует повышению содержания общего белка в сыворотке крови на 1,4% (P≤0,05), глюкозы — на 22,6% (P≤0,05) и снижению содержания холестерина на 12,3% (P≤0,05). The effect of the administration of different doses of the tissue biostimulant on the biochemical blood serum indices of replacement young cattle was studied. The experiment was carried out in the Altai Region in 4 groups of 10 Black-Pied replacement heifers of the Priobskiy type. When selecting the animals, their age (1 month) and live weight (51.3±1.48 kg) was taken into account. The experiment lasted 14 days. Saline solution was injected under the skin to the animals of the control group at a dose of 3.0 mL per 1 head; the tissue bio-stimulant was administered in the following doses: in the 1st trial group — 2.0 mL per head; the 2nd trial group 3.0 mL per head; the 3rd trial group — 4.0 mL per 1 head. The trial batch of the tissue bio-stimulant was made from velvet antler deer by-products and slaughterhouse offal by using the patent-protected technology. The tissue biostimulant was made from mesenteric lymph nodes and mediastinums, spleen, liver, uteri with 2—3 month old fetuses, and placentae collected under aseptic conditions from healthy animals at slaughter. The administration of the tissue bio-stimulant to heifers in different doses increased some of the studied biochemical blood serum indices. The tissue biostimulant dose of 3.0 mL per head should be considered the most optimal one; it increased the total protein content in blood serum by 1.4% (P≤0.05), glucose — by 22.6% (P≤0.05), and decreased cholesterol content by 12.3% (P≤0.05).