Phytoremediation of nickel by quinoa: Morphological and physiological response

The amount of soil contaminated with heavy metal increases due to urbanization, industrialization, and anthropogenic activities. Quinoa is considered a useful candidate in the remediation of such soil. In this pot experiment, the phytoextraction capacity of quinoa lines (A1, A2, A7, and A9) against different nickel (Ni) concentrations (0, 50, and 100 mg kg-1) were investigated. Required Ni concentrations were developed in polythene bags filled with sandy loam soil using nickel nitrate salt prior to two months of sowing and kept sealed up to sowing. Results showed that translocation of Ni increased from roots to shoots with an increase in soil Ni concentration in all lines. A2 line accumulated high Ni in leaf compared to the root as depicted by translocation factor 3.09 and 3.21 when grown at soil having 50 and 100 Ni mg kg-1, respectively. While, in the case of root, A7 accumulated high Ni followed by A9, A1, and A2, respectively. There was a 5–7% increased seed yield by 50 mg kg-1 Ni in all except A1 compared to control. However, growth and yield declined with a further increase in Ni level. The maximum reduction in yield was noticed in A9, which was strongly linked with poor physiological performance, e.g., chlorophyll a, b, and phenolic contents. Ni concentrations in the seed of all lines were within the permissible value set (67 ppm) by FAO/WHO. The result of the present study suggests that quinoa is a better accumulator of Ni. This species can provide the scope of decontamination of heavy metal polluted soil. The screened line can be used for future quinoa breeding programs for bioremediation and phytoextraction purpose.

Developing climate-responsive passive strategies for residential envelopes in the warm humid climate of South India

PurposeEnergy codes for residential buildings in India prescribe design guidelines for each climate zone. However, these guidelines are broad and similar for different cities under the same zone overlooking climatic variations due to altitude, location and other geographical factors.Design/methodology/approachTo develop strategies addressing the city-specific requirements, a stepwise simulation approach was used. Integrated Environmental Solutions–Virtual Environment (IES-VE) was used to create a prototype of a singly detached residence. The applicability of strategies is studied during the day and night times. Optimum orientation, the thickness of insulation, Window–Wall Ratio, the impact of cross-ventilation and shading depth are determined for two cities – Tiruchirappalli and Coimbatore under the warm-humid climate zone of India.FindingsResults indicate that optimum insulation thickness and WWR vary between both cities during daytime and night time. In Tiruchirappalli, roof and wall insulation using polyurethane board (100 mm) and foam concrete (25 mm) offers a maximum reduction of 2.2°C indoors. Foam concrete (25 mm) insulation for roof and expanded polystyrene (25 mm) for walls reduce a maximum of 2.6°C during daytime in Coimbatore. Further, night ventilation with 20% WWR allows an average decrease of 0.5–0.6°C in triply exposed spaces facing the South. The use of a 2'0" depth shading device shows a maximum reduction of 0.1–0.3°C.Originality/valueThe contribution of this work lies in developing city-specific inputs presenting the advantage of easy replicability for other cities in the Indian context.

Effect of shelterbelt on the microclimate of the gram (Cicer arietinum) crop

The shelterbelt influence on the microclimate of gram crop in different intercropping systems comprising of four treatment, viz., pure gram, 1 : 1, 2 : 1 and 4 : 1 gram and raya, was quantified. Albedo varied from 14.8 to 22.6 percent in various treatments with highest value fo 18.3 percent in pure gram treatment, whereas, photosynthetically active radiation absorption was highest in 2 : 1 gram treatment (76%). Maximum reduction (69.2 per cent) in wind speed was observed in 1 : 1 intercropping system at 4h distance from the edge of the shelterbelt in comparison to pure gram field. Relative humidity was 8 to 15 percent less in the sheltered gram as compare to the pure gram, whereas, the average air temperature increased by 1 to 2 C in the sheltered crop over that of the pure gram and this increase was in the range of 9.9 to 12.2 per cent at 4h distance from the edge of shelterbelt. The grass minimum temperature was higher by 0.7degree cent grate in 2 : 1 treatment over that of pure gram during the months of January and February.

NI-18 A Quantitative Analysis of Intraoperative 3T-Diffusion Tensor Imaging parameters for Functional Prediction with Motor Eloquent Glioma Patients

OBJECTIVE: In motor eloquent glioma surgery, the value of intraoperative diffusion tensor (iDT) imaging was not established to preserve motor function. This study aimed to investigate a relationship between postoperative motor function and iDT imaging parameters, including fractional anisotropy (FA), mean diffusivity (MD), and shortest distance (SD) from resected tumor margin to the corticospinal tract (CST) of gliomas in motor eloquent areas. METHODS: This retrospective study enrolled 20 patients with newly diagnosed supratentorial glioma who underwent surgery and intraoperative magnetic resonance imaging at our hospital. Patients were divided into two groups (i.e., worsening and non-worsening groups) based on their manual muscle test scores before and three months after surgery. We obtained the mean FA and MD values bilaterally, along with identification of the CST and determined the ratios (the affected side / the contralateral side). The SD was measured between the CST and the resected margin of the tumor. We evaluated the quantitative analysis of these parameters related to motor functional outcomes. Moreover the correlation was measured between these parameters and the maximum reduction rate of cortical motor evoked potentials (MEPs) during surgery. RESULTS: In the worsening group (n = 5), the mean FA ratio was lower and the mean MD ratio was higher compared with the non-worsening group (n = 15; P < 0.001 and P < 0.01, respectively). Cut-off values were 0.87 for FA and 1.08 for MD. SD was 7.95 mm in the non-worsening group and 0.44 mm in the worsening group (P < 0.01). These iDT based parameters, the mean FA ratio and the SD, were well correlated with the maximum reduction rate in MEP (R = 0.72 and 0.80, respectively). CONCLUSIONS: The mean ratio of FA, MD, and SD using iDT imagings predict postoperative motor function and help in optimal surgical planning in patients with motor eloquent glioma.

Reduced ice number concentrations in contrails from low-aromatic biofuel blends

Sustainable aviation fuels can reduce contrail ice numbers and radiative forcing by contrail cirrus. We measured apparent ice emission indices for fuels with varying aromatic content at altitude ranges of 9.1–9.8 and 11.4–11.6 km. Measurement data were collected during the ECLIF II/NDMAX flight experiment in January 2018. The fuels varied in both aromatic quantity and type. Between a sustainable aviation fuel blend and a reference fuel Jet A-1, a maximum reduction in apparent ice emission indices of 40 % was found. We show vertical ice number and extinction distributions for three different fuels and calculate representative contrail optical depths. Optical depths of contrails (0.5–3 min in age) were reduced by 40 % to 52 % for a sustainable aviation fuel compared to the reference fuel. Our measurements suggest that sustainable aviation fuels result in reduced ice particle numbers, extinction coefficients, optical depth and climate impact from contrails.

CTNI-43. CXCL12 INHIBITION IN MGMT UNMETHYLATED GLIOBLASTOMA – RESULTS OF AN EARLY PROOF-OF-CONCEPT ASSESSMENT IN THE MULTICENTRIC PHASE I/II GLORIA TRIAL (NCT04121455)

BACKGROUND Preclinical studies showed that CXCL12-mediated influx of highly angiogenic monocytes/macrophages is a key driver of tumor re-vascularization and re-growth after radiotherapy (RT) of glioblastoma (GBM). We report findings from a phase I/II proof-of concept (PoC) study on CXCL12 inhibition during and after RT of GBM. METHODS Patients ≥18years with incompletely or unresected GBM without MGMT promoter hypermethylation and ECOG≤2 were eligible to participate. Patients received continuous (24/7) i.v. infusions of 200mg/week (n=3), 400mg/week (n=3) or 600mg/week (n=3) of the CXCL12 inhibitor olaptesed pegol (OLA) for 26 weeks during and after normo- or hypofractionated RT (60Gy/40.05Gy). The primary endpoint was safety as per the incidence of treatment-related adverse events. The study was accompanied by PoC-research including multiparametric MRI biomarkers (relative cerebral blood volume, rCBV; fractional tumor burden with high perfusion, FTBhigh; apparent diffusion coefficient, ADC) and of multiplexed immunofluorescence imaging (CODEX®) of reference and patient samples. Initial results of these analyses are reported for the first six patients enrolled. RESULTS Five of six (83%) patients assessed with advanced MRI showed response under OLA in rCBV/FTBhigh and ADC. Maximum reduction in perfusion (rCBV) from baseline was 55%, maximum reduction of FTBhigh was 55% and maximum increase in ADC was 77%. Furthermore, five of six (83%) patients analyzed showed reduction of enhancing tissue volumes in at least one scan under OLA therapy. In both one patient and two reference samples CXCL12 co-localized with endothelial cells of the microvascular proliferation zone. In a paired sample (before/during OLA) of one patient, endothelial cells stained positive for CXCL12 before but not during treatment and almost all GBM cells were negative in Ki67 staining in the sample obtained under OLA therapy. CONCLUSIONS Advanced MRI and multiplexed immunofluorescence suggest efficacy of combined radiotherapy and CXCL12 inhibition in unmethylated GBM. Funded by NOXXON Pharma AG; ClinicalTrials.gov number, NCT04121455.

Discrete-Event Simulation to Model the Thrombolysis Process for Acute Ischemic Stroke Patients at Urban and Rural Hospitals

Background: Effective treatment with tissue plasminogen activator (tPA) critically relies on rapid treatment. Door-to-needle time (DNT) is a key measure of hospital efficiency linked to patient outcomes. Numerous changes can reduce DNT, but they are difficult to trial and implement. Discrete-event simulation (DES) provides a way to model and determine the impact of process improvements.Methods: A conceptual framework was developed to illustrate the thrombolysis process; allowing for treatment processes to be replicated using a DES model developed in ARENA. Activity time duration distributions from three sites (one urban and two rural) were used. Five scenarios, three process changes, and two reductions in activity durations, were simulated and tested. Scenarios were tested individually and in combinations. The primary outcome measure is median DNT. The study goal is to determine the largest improvement in DNT at each site.Results: Administration of tPA in the imaging area resulted in the largest median DNT reduction for Site 1 and Site 2 for individual test scenarios (12.6%, 95% CI 12.4–12.8%, and 8.2%, 95% CI 7.5–9.0%, respectively). Ensuring that patients arriving via emergency medical services (EMS) remain on the EMS stretcher to imaging resulted in the largest median DNT improvement for Site 3 (9.2%, 95% CI 7.9–10.5%). Reducing both the treatment decision time and tPA preparation time by 35% resulted in a 11.0% (95% CI 10.0–12.0%) maximum reduction in median DNT. The lowest median and 90th percentile DNTs were achieved by combining all test scenarios, with a maximum reduction of 26.7% (95% CI 24.5–28.9%) and 17.1% (95% CI 12.5–21.7%), respectively.Conclusions: The detailed conceptual framework clarifies the intra-hospital logistics of the thrombolysis process. The most significant median DNT improvement at rural hospitals resulted from ensuring patients arriving via EMS remain on the EMS stretcher to imaging, while urban sites benefit more from administering tPA in the imaging area. Reducing the durations of activities on the critical path will provide further DNT improvements. Significant DNT improvements are achievable in urban and rural settings by combining process changes with reducing activity durations.

Outdoor Thermal Comfort Optimization through Vegetation Parameterization: Species and Tree Layout

The optimization of outdoor thermal comfort has become the keystone to guarantee the healthy and comfortable use of outdoor spaces. This study aims to optimize the outdoor thermal comfort through vegetation parameterization in a boulevard located in Guelma city, Algeria during summertime. However, two main parameters were investigated, species and tree layout, through a numerical simulation. We first collected microclimate data of a sunny summer day. Second, we used real microclimate data in different simulations using the Envi-met atmospheric model. The findings reveal that Ficus Nitida is the most significant species to intercept solar radiation and provide shade over the day in Souidani Boudjemaa Boulevard, with a maximum reduction of Ta = 0.3 °C and UTCI = 2.6 °C at 13:00 p.m. Tree layout is a determining parameter in the creation of shaded paths, based on the quality of the shadows cast by the trees, namely, their size. Thereby, planting the washingtonia palm trees along the center of the boulevard is the best option to maximize the shaded area within the boulevard, with maximum reduction of Ta = 1.8 °C and UTCI = 3.5 °C at 16:00 p.m.

Facile Preparation and Characteristic Analysis of Sulfated Cellulose Nanofibril via the Pretreatment of Sulfamic Acid-Glycerol Based Deep Eutectic Solvents

A deep eutectic solvent (DES) composed of sulfamic acid and glycerol allowed for the sustainable preparation of cellulose nanofibrils (CNF) with simultaneous sulfation. The reaction time and the levels of sulfamic acid demonstrated that fibers could be swelled and sulfated simultaneously by a sulfamic acid-glycerol-based DES and swelling also promoted sulfation with a high degree of substitution (0.12). The DES-pretreated fibers were further nanofibrillated by a grinder producing CNF with diameters from 10 nm to 25 nm. The crystallinity ranged from 53–62%, and CNF maintained the original crystal structure. DES pretreatment facilitated cellulose nano-fibrillation and reduced the energy consumption with a maximum reduction of 35%. The films prepared from polyvinyl alcohol (PVA) and CNF showed good UV resistance ability and mechanical properties. This facile and efficient method provided a more sustainable strategy for the swelling, functionalization and nano-fibrillation of cellulose, expanding its application to UV-blocking materials and related fields.

Dynamics of tax revenues in the budget of Ukraine and their forecast during the crisis period

It is extremely important for the budget process to obtain accurate forecasts of potential tax revenues, especially in periods of disruption and crisis. The paper is devoted to the study of dynamics of tax revenues’ volumes in the budget of Ukraine and the forecast of their values during the crisis.The dynamics of tax revenues in the Consolidated Budget of Ukraine, studied by using randomized R|S-analysis, fractal and probabilistic analyses as well as entropy calculation based on the data on monthly tax revenues for the period 2011–2021, is anti-persistent, fractal-like and unpredictable based on parametric dependencies, simple and complex trends. The topological dimension of the lines of dynamics for tax revenues of all types of taxes is much higher than 1, and the Hirst index indicates either fractal similarity of dynamics or its chaos. The map of dissipation periods of tax revenues in Ukraine, determined on the basis of entropy calculation and periods of negative entropy production according to the dynamics of tax revenues, coincided with the periods of maximum reduction in their volumes. The most crisis periods in the formation of tax revenues are 2019–2020, for certain types of taxes – 2016–2020, but the dissipation of tax revenues is projected for 2021–2022.The comparison of the level of fractal similarity in dynamics of the volume of tax revenues and peculiarities of the dynamics of entropy and entropy production, allowed to substantiate the division of taxes into nine types, of which five were found in Ukraine.