Impact of Soil Chemical Properties on the Growth Promotion Ability of Trichoderma ghanense, T. tomentosum and Their Complex on Rye in Different Land-Use Systems

Microbial-based biostimulants that increase plant performance and ensure sustainable restoration of degraded soils are of great importance. The aim of the present study was to evaluate the growth promotion ability of indigenous Trichoderma ghanense, T. tomentosum and their complex on early rye seedlings in sustained grassland and arable soil. The impact of soil chemical properties on the ability of selected Trichoderma strains and their complex to promote plant growth was determined by the evaluation of the rye (Secale cereale L.) early seedling growth—measuring the length of shoots and roots as well as their dry weight. Trichoderma species were tested for their ability to produce extracellular degradative enzymes on solid media. Furthermore, the soil properties and CM-cellulase activity of soil were estimated. The indigenous Trichoderma strains possess the capacity to produce enzymes such as peroxidase, laccase, tyrosinase, and endoglucanase. The results indicated a significant (p < 0.05) increase in plant growth and the improvement of some soil chemical properties (total N, mobile humic and fulvic acids, exchangeable K2O, soil CM-cellulase activity) in inoculated soils when compared to the control. The growth of the roots of rye seedlings in sustained grassland was enhanced when T. tomentosum was applied (p = 0.005). There was an increase in total weight and shoot weight of rye seedlings when T. ghanense was used in the arable soil (p = 0.014 and p = 0.024). The expected beneficial effect of Trichoderma spp. complex on rye growth promotion was not observed in any tested soil. The results could find application in the development of new and efficient biostimulants, since not only do physiological characteristics of fungi play an important role but also the quality of the soil has an impact.

Effects of Trichoderma asperellum BV10 and Bacillus amyloliquefaciens BV03 in Meloidogyne incognita Control Considering Three Different Management Systems

Crop yield decrease is the main concern when a pathogen or plague is identified in an agriculture field. Thus, part of this issue can be attributed to plant-parasitic nematodes (PPNs), such as Meloidogyne species, due to, most of the time, the hard diagnosis, and non-specific symptoms. Its management is mainly based on chemical pesticides, followed by a few potential biological control agents, and the management system. Therefore, this study aimed to evaluate the effects of biological agents in Meloidogyne incognita control in different soil systems. For that, two biological products were chosen, Trichoderma asperellum BV10 and Bacillus amyloliquefaciens BV03, and soils were sampled from three different managements systems: (i) soybean no-tilled system at Goi&aacute;s state, Brazil; (ii) forest soil at Goi&aacute;s state, Brazil, and (iii) soybean conventional managed system at Mato Grosso do Sul state, Brazil. Biocontrol and growth promotion effects, volatile organic compounds (VOCs) and soil respiration were determined in vegetation house and laboratory, respectively. As a result, both BV10 and BV03 had significant nematode control activity, comparing to control treatment, in all the three soils systems. Plus, the number of immobile nematodes by potential VOCs had significant increase when BV03 was applied, while the application of BV10 agent raised the soil respiration rate. In conclusion, both biocontrol agents presented great efficiency in control M. incognita, with better performance of BV03. Lastly, more studies must be done to elucidate how the resident soil microbiome can influence on biocontrol agent establishment and performance, as well as the consequence of the application of biological products on soil microbiome network.

Perception of Biocontrol Potential of Bacillusinaquosorum KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis

Tomato Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against Fol was investigated through integrated genome mining and chemical analysis. Strain KR2-7 was identified as B. inaquosorum based on phylogenetic analysis. Through the genome mining of strain KR2-7, we identified nine antifungal and antibacterial compound biosynthetic gene clusters (BGCs) including fengycin, surfactin and Bacillomycin F, bacillaene, macrolactin, sporulation killing factor (skf), subtilosin A, bacilysin, and bacillibactin. The corresponding compounds were confirmed through MALDI-TOF-MS chemical analysis. The gene/gene clusters involved in plant colonization, plant growth promotion, and induced systemic resistance were also identified in the KR2-7 genome, and their related secondary metabolites were detected. In light of these results, the biocontrol potential of strain KR2-7 against tomato Fusarium wilt was identified. This study highlights the potential to use strain KR2-7 as a plant-growth promotion agent.

Exploiting Beneficial Pseudomonas spp. for Cannabis Production

Among the oldest domesticated crops, cannabis plants (Cannabis sativa L., marijuana and hemp) have been used to produce food, fiber, and drugs for thousands of years. With the ongoing legalization of cannabis in several jurisdictions worldwide, a new high-value market is emerging for the supply of marijuana and hemp products. This creates unprecedented challenges to achieve better yields and environmental sustainability, while lowering production costs. In this review, we discuss the opportunities and challenges pertaining to the use of beneficial Pseudomonas spp. bacteria as crop inoculants to improve productivity. The prevalence and diversity of naturally occurring Pseudomonas strains within the cannabis microbiome is overviewed, followed by their potential mechanisms involved in plant growth promotion and tolerance to abiotic and biotic stresses. Emphasis is placed on specific aspects relevant for hemp and marijuana crops in various production systems. Finally, factors likely to influence inoculant efficacy are provided, along with strategies to identify promising strains, overcome commercialization bottlenecks, and design adapted formulations. This work aims at supporting the development of the cannabis industry in a sustainable way, by exploiting the many beneficial attributes of Pseudomonas spp.

Plant Growth-Promoting Activity of Pseudomonas aeruginosa FG106 and Its Ability to Act as a Biocontrol Agent against Potato, Tomato and Taro Pathogens

P. aeruginosa strain FG106 was isolated from the rhizosphere of tomato plants and identified through morphological analysis, 16S rRNA gene sequencing, and whole-genome sequencing. In vitro and in vivo experiments demonstrated that this strain could control several pathogens on tomato, potato, taro, and strawberry. Volatile and non-volatile metabolites produced by the strain are known to adversely affect the tested pathogens. FG106 showed clear antagonism against Alternaria alternata, Botrytis cinerea, Clavibacter michiganensis subsp. michiganensis, Phytophthora colocasiae, P. infestans, Rhizoctonia solani, and Xanthomonas euvesicatoria pv. perforans. FG106 produced proteases and lipases while also inducing high phosphate solubilization, producing siderophores, ammonia, indole acetic acid (IAA), and hydrogen cyanide (HCN) and forming biofilms that promote plant growth and facilitate biocontrol. Genome mining approaches showed that this strain harbors genes related to biocontrol and growth promotion. These results suggest that this bacterial strain provides good protection against pathogens of several agriculturally important plants via direct and indirect modes of action and could thus be a valuable bio-control agent.

Microbiological services delivered by the pig gut microbiome

The gut microbiome plays a fundamental role in regulating pig health and growth. Understanding the functions performed by the microbiome is vital when considering it as a target to improve pig health and growth, a pursuit driven by the increasing regulation of traditional means of disease control and growth promotion. This chapter explores the structure, diversity and functions of the pig gut microbiome, focusing on the role of the resident bacterial communities. It examines their relationships, interactions, and contributions to the host, ranging from the production of antimicrobial substances and prevention of pathogen colonisation to improvement of nutrient digestibility and the production of volatile fatty acids (VFAs) and vitamins. The chapter also reviews bacterial communication and the antibiotic resistome of the pig gut, outlining how they may be targeted/manipulated to reduce antibiotic resistance and promote improved gut health.

The Role of Genetic Variation in Maize Response To Beneficial Endophytes

Growth-promoting endophytes have great potential to boost crop production and sustainability. There is, however, a lack of research on how differences in the plant host affect an endophyte’s ability to promote growth. We set out to quantify how different maize genotypes respond to specific growth-promoting endophytes. We inoculated genetically diverse maize lines with three different known beneficial endophytes: Herbaspirillum seropedicae (a gram-negative bacteria), Burkholderia WP9 (a gram-negative bacteria), and Serendipita vermifera Subsp. bescii (a Basidiomycota fungus). Maize seedlings were grown for 3 weeks under controlled conditions in the greenhouse and assessed for various growth promotion phenotypes. We found Herbaspirillum seropedicae to increase chlorophyll content, plant height, root length, and root volume significantly in different maize genotypes, while Burkholderia WP9 did not significantly promote growth in any lines under these conditions. Serendipita bescii significantly increased root and shoot mass for 4 maize genotypes, and growth promotion correlated with measured fungal abundance. Although plant genetic variation by itself had a strong effect on phenotype, its interaction with the different endophytes was weak, and the endophytes rarely produced consistent effects across different genotypes. This genome-by-genome interaction indicates that the relationship between a plant host and beneficial endophytes is complex, and it may partly explain why many microbe-based growth stimulants fail to translate from laboratory settings to the field. Detangling these interactions will provide a ripe area for future studies to understand how to best harness beneficial endophytes for agriculture.