Introduction:
Adult mammals, including humans, experience limited recovery following myocardial infarction (MI). Recently, our group has uncovered a unique ability for enhanced cardiac recovery in adult spiny mice (
Acomys
).
Methods and Results:
Our preliminary studies have established appropriate similarities in heart structure and coronary anatomy between
Acomys
and
Mus
. Following the same permanent left artery descending (LAD) ligation surgery,
Acomys
and
Mus
showed a similar degree of early cardiac injury. Echocardiography and histological analyses showed more robust cardiac recovery and smaller scar area in
Acomys
. Moreover,
Acomys
exhibited a higher survival rate compared to
Mus
with no evidence of cardiac rupture or hypertrophy (measured by increasing heart/body weight) after MI. To investigate the mechanisms underlying this phenomenon, we isolated and quantified the size, nuclei number, and ploidy of adult cardiomyocytes (CMs) in both species (n=5 animals/group) using the Langendorff perfusion technique.
Acomys
CMs are significantly smaller in size compared to
Mus
and
Acomys
hearts have higher percentage of mononucleated cardiomyocytes. Using flow cytometry, we found that
Acomys
possess a higher percentage of diploid CMs compared to
Mus
. A closer examination of cardiomyocytes using electrophysiology revealed the presence of a T-type calcium current in adult
Acomys
CMs; a characteristic which is usually found in highly proliferative embryonic ventricular cardiomyocytes. Lastly,
Acomys
CMs demonstrated higher proliferative rates after injury compared to
Mus
.
Conclusion:
Taken together, our data provide strong evidence for enhanced cardiac repair in an adult mammalian model. Our results strongly suggest that
Acomys
maintain proliferative adult cardiomyocytes as adults and thus provide a cellular mechanism for their enhanced cardiac recovery after AMI.