Draft Genome of Tanacetum Coccineum: Genomic Comparison of Closely Related Tanacetum-Family Plants

The plant Tanacetum coccineum (painted daisy) is closely related to Tanacetum cinerariifolium (pyrethrum daisy). However, T. cinerariifolium produces large amounts of pyrethrins, a class of natural insecticides, whereas T. coccineum produces much smaller amounts of these compounds. Thus, comparative genomic analysis is expected to contribute a great deal to investigating the difference in biological defense systems, including pyrethrin biosynthesis. Here, we elucidated the 9.4-Gb draft genome of T. coccineum, consisting of 2,836,647 scaffolds and 103,680 genes. Comparative analyses of the draft genome of T. coccineum and that of T. cinerariifolium, generated in our previous study, revealed distinct features of T. coccineum genes. While the T. coccineum genome contains more numerous ribosome-inactivating protein (RIP)-encoding genes, the number of higher-toxicity type-II RIP-encoding genes is larger in T. cinerariifolium. Furthermore, the number of histidine kinases encoded by the T. coccineum genome is smaller than that of T. cinerariifolium, suggesting a biological correlation with pyrethrin biosynthesis. Moreover, the flanking regions of pyrethrin biosynthesis-related genes are also distinct between these two plants. These results provide clues to elucidation of species-specific biodefense systems, including the regulatory mechanisms underlying pyrethrin production.

Identification of multiple odorant receptors essential for pyrethrum repellency in Drosophila melanogaster

Pyrethrum extract from dry flowers of Tanacetum cinerariifolium (formally Chrysanthemum cinerariifolium) has been used globally as a popular insect repellent against arthropod pests for thousands of years. However, the mechanistic basis of pyrethrum repellency remains unknown. In this study, we found that pyrethrum spatially repels and activates olfactory responses in Drosophila melanogaster, a genetically tractable model insect, and the closely-related D. suzukii which is a serious invasive fruit crop pest. The discovery of spatial pyrethrum repellency and olfactory response to pyrethrum in D. melanogaster facilitated our identification of four odorant receptors, Or7a, Or42b, Or59b and Or98a that are responsive to pyrethrum. Further analysis showed that the first three Ors are activated by pyrethrins, the major insecticidal components in pyrethrum, whereas Or98a is activated by (E)-β-farnesene (EBF), a sesquiterpene and a minor component in pyrethrum. Importantly, knockout of Or7a, Or59b or Or98a individually abolished fly avoidance to pyrethrum, while knockout of Or42b had no effect, demonstrating that simultaneous activation of Or7a, Or59b and Or98a is required for pyrethrum repellency in D. melanogaster. Our study provides insights into the molecular basis of repellency of one of the most ancient and globally used insect repellents. Identification of pyrethrum-responsive Ors opens the door to develop new synthetic insect repellent mixtures that are highly effective and broad-spectrum.

Weed Community in a Conventionally-Grown Olive Orchard Vs. Weed Community in Consociation with Pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.)

On the experimental plots of the Institute of Agriculture and Tourism in Poreč, weed communities were analyzed in a conventionally‐grown olive orchard and in consociation with pyrethrum. Phytocenological surveys were conducted in March, June, and September using the Braun-Blanquet Cover Abundance Scale. Altogether, 54 species of vascular plants were recognized in both orchards. However, in a conventionally‐grown olive orchard, 27 were identified, whereas 42 weed species were identified in consociation with pyrethrum. Only 15 weeds were common to both orchards. Between both olive orchards, significant differences existed regarding the floristic and community diversity metrics. In consociation with pyrethrum as a cover crop, olive orchard had a more stable weed community during the season, while two weed species, Mercurialis annuua L. and Medicago arabica (L.) Huds., dominated in a conventionally‐grown orchard in the fall. The weed communities in both orchards significantly differ considering the appearance season (spring, summer, and fall). An autumnal weed community in a conventionally‐grown olive orchard was more diverse, more abundant, and with a more distinct floristic composition than a weed community in the spring and summer periods. However, an olive‐orchard weed community in consociation with pyrethrum also differed regarding the season, but a Canonical Correspondence Analysis (CCA) demonstrated a clear separation of a more diverse estival weed community than the one observed in the spring and summer.