This study was designed to assess the access of Nepalese farmers to the training and extension service, gender division on agricultural work, and factors affecting agrobiodiversity management activities. A total of 2,817 respondents were interviewed at different locations throughout Nepal. The information was collected using the mWater surveyor. Descriptive and inferential analyses were done. The respondents having received training in agriculture were significantly higher among elite, educated, and agricultural households. Access to extension facilities was significantly determined by the type of household, ethnicity, occupation, and education of respondents. Male domination in the choice of crops, land preparation, and seed selection were significantly higher in male-headed households, marginalized groups, and agricultural households. However, females were more likely to be involved in seed sowing. The male domination in male-headed households were significantly higher for applying fertilizers, weeding, irrigation, and pest control. Among elite ethnic groups, domination of males was significantly higher for fertilizer application. The role of the male in agricultural households was significantly higher in all aspects. One unit increase in the area increased the likelihood of male involvement in irrigation by 30%. The males are likely to be more involved in harvesting, sales of products, and control of income. Elite and educated respondents coupled with access to training practiced more crop rotation compared to the rest. The likelihood of practicing intercropping and mixed cropping was influenced by extension facilities and training facilities. Elite groups and farmers with extension facilities tended to practice more agroforestry. So, the types of households, education, and ethnicity have a key role in the gender differentiation in agriculture operation. Moreover, training and extension facilities help a lot in the conservation and practice of agrobiodiversity. There is an urgent need in improving the women's role and overall management of the agricultural landscape.