Gastrointestinal Helminth Parasites of Wild Ungulates In Hirpora Wildlife Sanctuary, Kashmir, India

Parasitic infection represents an emerging threat to wild ungulates and a challenge to their management. Although a lot of work has been carried out on helminth parasitic infestation of domestic ungulates of Kashmir but the data pertaining to this aspect of wild ungulates has being ignored. The study on gastrointestinal helminth parasitic infestation of wild ungulates was carried out during post livestock grazing period (November to May) of 2018/2019 in Hirpora Wildlife sanctuary (HWLS) to fill the gap in the existing literature. During the study fresh faecal samples of musk deer Moschus sp.(n=44) and markhor Capra falconeri (n=41)were collected and examined qualitatively and quantitatively for gastrointestinal helminth parasites. A total of seven helminth parasites were recorded which are arranged in the descending order of their overall prevalence as Haemonchus spp. (44.70%),Nematodirus spp. (40%), Trichuris spp. (37.64%), Strongyloides spp. (34.11%)Trichostrongylus spp. (28.23%),Monieziaspp. (23.52%) and Fasciola spp. (20%). The mean EPG (eggs per gram) of different parasites showed a considerable variation in both the wild ungulates. The highest mean EPGwas that of Haemonchus spp. and the lowest mean EPG was that of Fasciola spp. in both hosts. A statistically significant difference was observed in the mean EPG of different parasites among two wild hosts (t=3.606, p=0.01).

The Influence of Age and Winter Environment on Protein Block Intake Behavior of Beef Cattle Winter Grazing Mixed-Grass Rangelands

This study evaluated the influence of cow age and temperature adjusted for windchill (Twindchill) on supplement intake behavior of cattle winter grazing rangelands. A mixed-age herd of Angus-based cows (291 and 316 in year 1 and 2, respectively) were classified by age (1-, 2-, 3-, 4-, 5–7-, and ≥8-year-old) and grazed rangeland pasture (329-ha) for two consecutive winters. Cows were provided free-choice access to supplement protein blocks in a SmartFeedPro self-feeder system. An Onset HOBO Weather Station collected data throughout the grazing period. Supplement intake (expressed as kg∙d−1 and g∙kg body weight−1), the coefficient of variation in supplement intake and intake rate (g∙min−1) displayed Twindchill × cow age × year interactions (p ≤ 0.02). In general, cow age displayed a quadratic effect on all supplement intake variables (p ≤ 0.04), where 3- to 4-year-old cattle had the greatest supplement intake with the least variation, while yearling cattle had the least amount of supplement intake and the greatest variation. In conclusion, winter environmental conditions interacted with cow age to affect cattle supplement intake behavior and, consequently, the efficacy of nutrient delivery systems for beef cattle winter grazing on rangelands.

Grazing Sheep in Organic Vineyards: An On-Farm Study about Risk of Chronic Copper Poisoning

Many winegrowers and sheep breeders are interested in wintertime grazing in vineyards, as an agroecological alternative to mowing or herbicide spraying, and additional supply of forage. Still, strong concern is raised by the use of copper-based fungicides, particularly in organic vineyards, since copper is known to induce chronic toxicosis in sheep. We conducted an on-farm study with n = 12 1-year-old Merinos × Mourerous ewes grazing the cover vegetation of vineyard plots during wintertime, in order to check whether this agricultural practice might be harmful to sheep. Our results indicate that most copper found in the cover vegetation originates from fungicide spraying versus plant uptake from the soil, and that rain-induced washing-off and plant growth-triggered dilution of copper are crucial to reach close-to-safe grazing conditions. Furthermore, we found that while sheep remained globally healthy during the 2 months of the experimental period, the plasma activity of Glutamate Dehydrogenase increased by 17.3 ± 3.0 U/L upon vineyard grazing (p < 0.001), reflecting liver storage of copper. We also discovered that the dynamics of molybdenum in sheep plasma are strongly affected by exposure to copper, suggesting a possible adaptation mechanism. Overall, our results suggest that winter grazing of sheep in organic vineyards is reasonably safe, but that care should be taken about grazing period duration. More research should be conducted with respect to long-term copper accumulation, spring and summer grazing, and possible protective mechanisms against copper chronic poisoning.

Intake and digestibility of nutrients during the grazing period in sheep on deferred marandu pastures with four initial heights

The objective of this study was to verify the effect of reducing in pasture height at the beginning of the deferment period (PHBD) of Brachiaria brizantha cv. Marandu on nutrient intake and digestibility in sheep during winter. The combinations between average sward heights (15, 25, 35 and 45 cm) at the beginning of the deferment period and the times of sward use during the winter were evaluated; intake and digestibility evaluations were carried out at the beginning, middle and end of the grazing period, which lasted 90 days. The experimental design used was completely randomized, with three replications. The parameters evaluated were: intake and digestibility of dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) and potentially digestible dry matter (pDDM). The pDDM contents were similar in pastures kept with 15 and 25 cm, but higher than those observed in pastures with 35 and 45 cm at the beginning of the deferment period. The NDF contents were lower and the CP and NDF digestibility were higher in 15 and 25 cm deferred pastures than in 35 cm at the beginning of deferment period, except for pastures lowered to 45cm. The improvement in nutritional value of the forage apparently ingested by sheep, promoted by ISP reduction, did not result in a variation in pasture consumption by the animals. DMI, expressed as percentage of body weight (%BW), ranged from 1.10 to 1.63. At the beginning of the grazing period, higher values of pDDM, CP and DM digestibility (DMD) were verified in simulated grazing samples. The same response pattern observed for nutritional value traits also occurred for DMI (g.day-1 and %BW) and CP intake. These were higher at the beginning, than at the middle and end of the grazing period. The use of lower sward heights at the beginning of the deferment period allowed the production of pasture with better nutritional quality, but these changes in the nutritional value of the pasture were insufficient to result in higher nutrient intake and digestibility. Furthermore, there was a reduction in nutritional value of the forage apparently consumed by sheep, in the intake and digestibility of deferred pastures throughout the grazing period, in winter. The lower pastures at the beginning of the deferment period (15 cm) improves the nutritional value of forage and nutrient digestibility at the beginning of the winter grazing period. The nutritional value and intake of deferred forage by sheep are compromised by the grazing period.

PSXI-13 Effects of pasture grazing on growth performance and blood composition of Hanwoo steer during the growing stage

This study investigated the effect of pasture grazing on growth performance and blood characteristics in nine-month-old Hanwoo (native Korean cattle) steers. Thirty one steers were randomly assigned to three groups: (CON) Italian ryegrass and concentrate (1.6% BW) until 14 month of age, (G1) pasture grazing 101 days without concentrate, (G2) pasture grazing 101 days and concentrate (1.6% BW). Initial BW of CON, G1 and G2 were 255 kg, 238 kg and 254kg respectively. Average daily gains were higher in CON (1.02 kg) and G2 (0.92 kg) than G1 (0.62 kg) (p &gt;0.05). Final weight and total weight gain were higher in the feeding concentrate (1.6% BW) groups (Con and G2) compared to the G1. Blood characteristics, such as such as total cholesterol (T-CHO), triglycerides (TG) and non-esterified fatty acid (NEFA) were investigated to test the effects on health and energy metabolism. T-CHO, an index of fat metabolism and liver function, was lower in Con (119.75 md/dL) and G2 (107.13md/dL) than G1 (87.79 mg/dL). TG was higher in Con (21.63 IU/L) than G1(15.14 IU/L) (P&lt; 0.08). NEFA was higher in G2 (384 uEq/L) than other groups (Con 138 uEq/L and G1 225 uEq/L). These results show that concentrates supplementation during grazing period is needed to increase BW of Hanwoo steer in the growing state.

PSXI-18 Nitrogen fertilization management of winter pastures for stocker cattle production

Bermudagrass is often over-seeded with a cool-season annual grass for grazing purposes. In the Gulf Coast region, summer perennials will not become dormant until there are low soil and ambient temperatures. Nitrogen fertilizer is an expensive input and its split application for winter pastures is recommended. The objective of this experiment was to evaluate the time of application of N fertilizer or its substitution with red clover as a source of N. Three treatments were evaluated in 3 consecutive years (2 replicates per year) on annual ryegrass (cv. Marshall) no-tilled planted (45 kg/ha) on Tifton-85 bermudagrass: 1) Urea in late November and mid-late January; 2) Red clover planted with annual ryegrass; 3) Urea in January only. Thirty crossbred steers (203±24 kg) were blocked by BW and randomly assigned to 1 of 6 groups. Each group were continuously stocked on 1.34 ha pastures for 90 d. Forage mass, botanical composition, and nutritive value of the pastures were determined from the time of the first N application and every 15 d thereafter. There was treatment x year interaction (P = 0.03) in botanical composition. Greater proportions (P = 0.03) of bermudagrass were present on the 2-application treatment on every sampling date until d 15 of the grazing period in Year 1 and 3. This was a result of N absorbed by bermudagrass which impacted its nutritive value (P = 0.01) although not forage production (P = 0.07). In Year 2, annual ryegrass dominated the swards due to a cold artic front in early November. Steers grazing fertilized treatments had greater gains (P = 0.04), while production per ha was greater (P = 0.03) when N was applied in January than when no N was applied. Hay production (kg DM/ha) was similar (P = 0.06) among treatments. Opportunities for improved economics of production and enhanced sustainability appear to be realistic objectives with changes in fertilization strategy.

PSI-4 Animal and agronomic performance while grazing winter stockpiled novel endophyte tall fescue established with three pasture renovation strategies

Comparing animal and agronomic performance following renovation of toxic-infected tall fescue (TF) to novel endophyte tall fescue (NE) is fundamental to evaluating economic returns and increasing adoption of NE. In 2018, three NE renovation strategies were implemented in a randomized complete block design in Bahama, NC. Strategies included: 1) control (C), 2) renovation to NE after one season of a single specie cover crop (1-SM), 3) renovation to NE after three seasons of a single specie cover crop (3-SM), and 4) renovation to NE after three seasons of a multi-specie cover crop (3-CM). Sixty-four Angus heifers (initial BW 290 kg ± 88) were randomly assigned to treatments and strip-grazed stockpiled fescue for 56-d, two years after NE establishment. Forage yield and nutritive value was measured every 14-d. Data were analyzed using proc GLIMMIX of SAS v9.4. Average daily gain was greatest (P = 0.0024) for cattle grazing 1-SM (0.67 kg/d), and least for 3-CM (0.54 kg/d) and C (0.44 kg/d), which did not differ. 3-SM (0.57 kg/d) did not differ from other treatments. Within the grazing period, forage yield was greatest (P = 0.0001) in 3-CM, 3-SM, and C compared to 1-SM (3053, 2941, 2856 and 2465 kg/ha, respectively). Prior to the initiation of grazing, crude protein concentration was greater (P = 0.0089) for 3-CM (17.5%) compared to C, 1-SM, and 3-SM (15.8%, 15.8% and 15.3%, respectively). Neutral detergent fiber was greater (P = 0.0135) for 3-SM (57%) than C (55.1%) and 3-CM (54.5%), but did not differ from 1-SM (54.6%). Concentration of total digestible nutrients was greater (P = 0.0187) for 3-CM (73.2%) compared to 1-SM (72.1%) and 3-SM (71.9%), but did not differ from C (72.5%). Data suggest animal and agronomic performance for NE was improved compared to TF during winter stockpile grazing in years following renovation.

High biodiversity in a limited mountain area revealed in the traditional production of Historic Rebel cheese by an integrated microbiota–lipidomic approach

Historic Rebel (HR) cheese is an Italian heritage cheese, produced from raw milk during the summer grazing period in the Alps. The aim of this work was (i) to characterize the cheese microbiota, by 16S rRNA gene amplicons sequencing, and the volatile and non-volatile lipophilic fraction, by Gas Chromatography and Dynamic Headspace Extraction-Gas Chromatography-Mass Spectrometry, and (ii) to evaluate their respective associations. HR cheese was dominated by Firmicutes phylum (99% of the entire abundance). The core microbiota was formed by Streptococcus, Lactobacillus, Lactococcus, Leuconostoc and Pediococcus genera together representing 87.2–99.6% of the total abundance. The polyunsaturated fatty acids composition showed a high PUFA n-3, PUFA n-6 and CLA content, two fold higher than typical plain cheeses, positively correlated with pasture altitude. A complex volatilome was detected, dominated in terms of abundance by ketones, fatty acids and alcohols. Total terpene levels increased at higher altitudes, being the main terpenes compounds α-pinene, camphene and β-pinene. The HR cheese showed a great diversity of bacterial taxa and lipophilic fractions among producers, despite belonging to a small alpine area, revealing a scarce cheese standardization and a chemical fingerprint of a typical mountain cheese produced during the grazing period. A deeper knowledge of the variability of HR cheese due to its composition in microbial community and volatile compounds will be appreciated, in particular, by elite consumers looking for niche products, adding economic value to farming in these alpine areas.

PSI-2 Spices in Free-choice Mineral Offer Gain Advantages to Cattle on Tallgrass Native Range and Promise as Fly Control

This study aims to evaluate effectiveness of two operational management systems for steer gains and fly control. The first strategy evaluated was pasture burn date of March (MAR) or April (APR). The second management strategy was fly control through feeding free-choice mineral with spices (SPICE) or without spices (CON). Eight pastures (n = 281 steers; initial weight 277.7 ± 25.2 kg) were used in a 2 x 2 factorial treatment structure. Steers were weighed individually, randomly assigned to treatment, and grazed for 85 days. Weekly 33% of steers per pasture were photographed and fly numbers counted. ADG was impacted by both burn date and mineral type (P = 0.01). Cattle on the APR-SPICE treatment had a greater ADG than MAR-SPICE and APR-CON with MAR-CON intermediate. Cattle on SPICE gained 4.5 kg (P = 0.12) more than cattle consuming CON mineral. Flies per steer were impacted by burn x mineral x week (P &lt; 0.001). In general, APR-SPICE steers had a greater number of flies weeks 8, 10, and 11 (P &lt; 0.05), corresponding to a time when mineral intake averaged 72% of the formulated intake. The MAR-SPICE steers consumed on average the formulated amount of mineral and through the entire grazing period had lower number of flies than APR-SPICE steers (P &lt; 0.05). The second largest ADG observed with the MAR-CON treatment may be attributed to lower fly numbers as this treatment had one extra week below economic threshold for horn flies. These results are somewhat inconclusive on pasture burn date, but show that the addition of spices to a free-choice complete mineral shows promise as a cost-effective method to increase gains in stocker steers on tallgrass native range. As a fly control method, the spices utilized in this study may be effective if intake is at formulated level.

Tamegrass Forage Options for Growing Heifers During the Summer

Developing methods to provide high quality forage through a majority of the year is important for cattle operations. The purpose of this study was to determine forage management options to off-set the summer “slump” with fescue. Four grass pasture treatments (10 pastures total; 1.67 ha each) were used in a completely randomized design and stocked with growing heifers (initial wt 190 ± 9.1 kg). Pasture treatments consisted of fescue (FES; n = 2 pastures with 4 head/pasture), crabgrass (CRAB; n = 3 pastures with 4 head/pasture), bermudagrass (BERM; n = 3 pastures with 5 head/pasture), and sorghum-sudan interseeded into fescue (SS-FES; n = 2 pastures with 6 head/pasture from April to July, then 4 head/pasture from July to November). Heifers were weighed in April, May, July, September, and November. Heifers assigned to FES/SS-FES pastures grazed from April to November (213 d) and for CRAB and BERM treatments, May through September (131 d). Heifers on FES grazed continuously, while other grass treatments were rotationally grazed. Heifers remained in treatment pasture through the entire grazing period. Data were analyzed using mixed procedures of SAS with pasture as experimental unit. Average daily gain for the entire grazing period was greater for heifers on SS-FES as compared to all other grass treatments (P = 0.001). Between April and May, FES heifers had greater ADG than SS-FES (P = 0.001); yet, heavier stocking rate in SS-FES resulted in similar BW gain/ha (P = 0.16). May to July ADG and BW gain/ha was greatest for BERM, then CRAB, with FES and SS-FES having the lowest gains (P &lt; 0.001). From July through September, ADG was greater for SS-FES and CRAB as compared to FES, with BERM intermediate (P = 0.03); and BW gain/ha tended to be lower for FES compared to other treatments (P = 0.10). Average daily gain and BW gain/ha were greater for SS-FES than FES (P = 0.001) from late September to November. As a summer grazing option, warm season grass alternatives, either as the sole source of pasture or interseeded into fescue, are better options for gain as compared to fescue alone.