epididymal spermatozoa
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Andrologia ◽  
2022 ◽  
Author(s):  
Lizbeth Juárez‐Rojas ◽  
Fahiel Casillas ◽  
Alma López ◽  
Miguel Betancourt ◽  
Mohammad Mehdi Ommati ◽  
...  

2022 ◽  
Vol 34 (2) ◽  
pp. 256
Author(s):  
S. Stoddard ◽  
J. Linn ◽  
A. Lemma ◽  
G. Wirtu

2021 ◽  
Vol 0 (0) ◽  
pp. 161-173
Author(s):  
Eman Fayez ◽  
Ali Salama ◽  
Zaher Rawash ◽  
Mohamed El Sayed

Author(s):  
Faith Chinonye Eke ◽  
Terzungwe Ahemen

Post mortem spermatozoa recovery is an important tool for harvesting germplasm. The study aimed to determine the effect of cold storage time at 4°C for 48 and 72 hours on the quality of sperm of Red Sokoto buck caudal epididymaly spermatozoa in terms of motility and livability recovered postmortem. Fifteen testicles with attached epididymis were collected and processed at 0, 48, and 72 hours of storage. The samples were divided into 3 groups according to the time processing after collection ; 0 hour, 48 hours and 72 hours, with a total of 5 samples per group. Each storage group motility and livability was evaluated, mean and the standard error of their mean was recorded. Mean percent sperm motility declined significantly ( p< 0.05) as postmortem storage time increased ( motility: 79.00+/- 2.92%, 40.00+/- 4.18, 37.00+/- 3.39) .There was no significant (p> 0.05) decrease in livability from the 0 hour storage to the 48 hour and 72 hours storage ( 75.60+/- 4.40, 73.60+/- 3.84, 68.20+/- 2.51). The current study has shown that viable spermatozoa can be recovered after 48 hours of storage at 4˚C of the cauda epididymides Red Sokoto buck.


Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2961
Author(s):  
Hiba Ali Hassan ◽  
Guillaume Domain ◽  
Gaia Cecilia Luvoni ◽  
Rana Chaaya ◽  
Ann Van Soom ◽  
...  

Canine and feline epididymal semen provide an additional source of gametes to preserve the genetics of valuable breeding dogs and tomcats, especially for those that fail to ejaculate, need castration as a therapy or die unexpectedly. Moreover, since it is quite common to perform castration of non-breeding dogs and cats, the development of a gene bank of epididymal semen collected after castration would greatly contribute to increase the genetic diversity in dogs and cats. Collection and cryopreservation of epididymal semen necessitates a full understanding of the function of the epididymis and of the characteristics of epididymal spermatozoa as opposed to ejaculated semen. During collection of epididymal semen, specific factors may have a negative effect on epididymal semen quality and freezability. Accordingly, the elimination of these triggers could enhance epididymal semen freezability and consequently positively influence post-thaw semen quality and outcome for different ARTs.


Toxicology ◽  
2021 ◽  
pp. 152886
Author(s):  
Felipe Couto-Santos ◽  
Arabela Guedes de Azevedo Viana ◽  
Ana Cláudia Ferreira Souza ◽  
Alexandre Augusto de Assis Dutra ◽  
Tiago Antônio de Oliveira Mendes ◽  
...  

Author(s):  
Ruhi Kabakci ◽  
Abdulkadir Kaya ◽  
Ayse Arzu Yigit ◽  
Omer Varisli

AbstractThis study is the first to investigate the effects of tebuconazole (TEB) on the physiological functions of bovine testicular cells and epididymal spermatozoa. Motility and plasma membrane integrity of spermatozoa exposed to TEB (0.001–100 µM) were evaluated at different incubation times (0–6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from the parenchyma of bovine testes, were seeded at 1.0 × 104 and 1.5 × 106 cells/well in 96- and 12-well culture plates and incubated for 48 h in culture media containing TEB (0.001–100 µM) to evaluate cytotoxicity and hormone release, respectively. TEB did not affect the motility and plasma membrane integrity. However, significant spermiotoxicity occurred at higher TEB (1–100 µM) concentrations (P < 0.05) compared to control and lower doses. Although no dose caused cytotoxicity in testicular cells (P > 0.05), 1 and 100 µM TEB caused a significant increase in testosterone secretion (P < 0.05). As a result, high doses of TEB (1–100 µM) had slightly suppressive effects on spermatozoa; however, these doses had stimulatory effects on testosterone secretion by testicular cells. It appears that the disruption of hormonal homeostasis of testicular cells after TEB exposure may result in metabolic and especially reproductive adverse effects in bulls.


2021 ◽  
pp. 1602-1607
Author(s):  
Surya Agus Prihatno ◽  
Yosua Kristian Adi ◽  
Teguh Budipitojo ◽  
Topas Wicaksono Priyo Jr ◽  
Yonathan Alvin Maruli Asi Sihotang

Background and Aim: In the male reproductive system, the aging process can lead to infertility. Recently, placenta and its derivatives have been researched as regenerative agents. This study aimed to describe the basic components of dried bovine placenta powder and its potential effects as a regenerative agent in a rat model of male reproductive aging with D-galactose induction. Materials and Methods: We divided 15 male Wistar rats, 2 months of age, into three groups: A, the health control group; B, the D-galactose induction group, and C, the D-galactose induction and 10% dried bovine placenta supplementation group. We measured epididymal sperm concentration and testicular weight and volume and analyzed these using one-way analysis of variance. Results: Dried bovine placenta was rich in nutrients, with 61.98% protein, 21.25±2.07 carbohydrates, 8.58% water, 4.93% ash, and 3.27% fat. The mean epididymal spermatozoa concentration of the rats in Groups A, B, and C was 3026×106/mL, 1492.8×106/mL, and 2732.5×106/mL, respectively. The average total testicle weights were 2.44 g, 2.72 g, and 2.57 g, respectively. The average total testicle volumes were 2.29 cm3, 2.49 cm3, and 2.33cm3, respectively. Conclusion: Dried bovine placenta powder is rich in nutrients, especially protein. Supplementation with dried bovine placenta can improve epididymal spermatozoa concentration that is important in fertility.


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