EFFECT OF ADDITION OF TEMULAWAK EXTRACT (Curcuma xanthorriza) ON ARTIFICIAL FEED ON GURAMI FISH GROWTH (Osphronemus goramy)

The problem with gouramy cultivation was slow growth due to the short intestines of gouramy so that the absorption of feed takes longer and less. Several previous studies used temulawak in the form of flour, extract, oil which was applied to carp, tilapia, milkfish, catfish. The addition of extract, temulawak flour to the feed gave an increase in growth both in length and weight in the test fish. The purpose of this study was to determine whether or not the effect of temulawak extract on the growth of gouramy fish. This research was conducted for 45 days. This research was conducted by experimental method using a completely randomized design (CRD) with 5 treatments (P) 3 times replication (U), in order to obtain 15 experimental units. Treatment 0 Control (without temulawak extract), Treatment 1 Provision of temulawak extract with a concentration of 14%/kg feed, Treatment P2 Administration of temulawak extract with a concentration of 16%/kg feed, treatment P3 Administration of temulawak extract with a concentration of 18%/kg feed, and treatment P4 administration of temulawak extract with a concentration of 20%/kg feed. The results showed that in the P0 treatment the absolute weight gain was 0.78 grams, in the P1 treatment the absolute weight was 0.58 grams, the P2 treatment was 0.33 grams, the P3 treatment was 0.37 grams, and in the P4 treatment it was obtained The result of absolute weight gain was 0.39 grams. The results showed the absolute length increase ranged from 0.35-0.57 cm. It can be concluded that the addition of temulawak extract to the commercial feed of carp has no significant effect on absolute weight, absolute length, survival rate and feed efficiency level.

The effect of chitosan levels in feed on the growth and daily feed consumption of giant gourami (Osphronemus goramy)

Chitosan is an ingredient that can be used as a feed additive. In aquaculture, chitosan can be used as an antimicrobial, antifungal, antioxidant growth-promoting and immunostimulant. This study aims to determine the optimum addition of chitosan to feed to increase growth and daily feed consumption of giant gourami. The method used in this research is an experimental method using a completely randomized design (CRD), which consists of five treatments and three replications. The treatments used were (A) without chitosan (control), (B) 2.5 g/kg feed, (C) 5 g/kg feed, (D) 7.5 g/kg feed, and (E) giving chitosan of 10 g/kg feed. The test fish used were giant gourami with a 6 – 8 cm length and a weight of 8 – 10 g. The container used was hapa measuring 1 x 1 x 1 m3 with a density of 15 fish per treatment and 42 days of maintenance. The feeding rate was 3% of the fish biomass. Data from observations of length and weight were analyzed using Analysis of Variance (ANOVA) with a 95% confidence level; if there was a significant difference, Duncan's multiple distance test was performed. The results showed that addition of chitosan 7.5 g/kg feed was effective in improving growth on giant gourami. During maintenance, absolute length growth was 2.90 ± 0.10 cm, absolute weight growth was 15.33 ± 1.19 g, daily growth was 36.51 ± 2.84%, feed conversion ratio was 1.69 ± 0.06, and survival rate was 100 ± 0.00%. This research concluded that the optimum level of addition of chitosan to increase growth and daily feed consumption of giant gourami was 7.5 g/kg feed.Keywords:ChitosanFeed additiveGiant gouramiGrowth

Chemistry analytical, fish micronuclei and onion chromosome damage for assessing treatment effect SBR in the Domžale–Kamnik central WWTP and quality of river Kamniška Bistrica

The aim of this study was to evaluate the cytotoxic and genotoxic potential of the wastewater (WW), the effectiveness of the treatment used by the wastewater treatment plant (WWTP) with sequential batch reactors (SBR) technology, and whether its final treated effluent (FTE) can compromise the water quality of the river at the location where it is discharged. We focused our research on six examples. For analytical chemistry and Allium metaphase (M) test all six samples were collected. Of these, three are so-called biotechnological patterns (WW, WW after mechanical step treatment and FTE), and three are natural riverine environmental patterns. For the micronucleus (MN) test, fish specimens were collected from three sites in the river Kamniška Bistrica. The first two sites locations are up and down the FTE outlet. Results from these areas were compared to the third site (not polluted) reference site, the so-called natural control group. Complementary study with analytical chemistry and biological tests shows that the treatment effect SBR in the Domžale–Kamnik central WWTP carried effectively proved to be efficient for the removal of the cytogenotoxic substances in treated effluent and consequently in aquatic environment.

Validation of the three-chamber strategy for studying mate choice in medaka

The three-chamber experiment, in which one test animal can choose between two animals placed in physically inaccessible compartments, is a widely adopted strategy for studying sexual preference in animals. Medaka, a small freshwater teleost, is an emerging model for dissecting the neurological/physiological mechanisms underlying mate choice for which intriguing findings have been accumulating. The three-chamber strategy has rarely been adopted in this species; therefore, here we investigated its validity using medaka colour variants that mate assortatively. First, a total of 551 movies, in which a test male and two choice females interacted for 30 min under a free-swimming condition, were manually analysed. The sexual preference of the males, calculated as a courtship ratio, was highly consistent between human observers (r > 0.96), supporting the objectivity of this manual-counting strategy. Second, we tested two types of three-chamber apparatuses, in which choice fish were presented in either a face-to-face or side-by-side location. Test fish (regardless of sex) spent most of the time associating with choice fish in the compartments. However, their sexual preference, calculated as an association ratio, was poorly reproduced when the locations of the choice fish were swapped. Third, the sexual preferences of males quantified using the manual-counting and either of the three-chamber strategies did not correlate (r = 0.147 or 0.297). Hence, we concluded that, even for individuals of a species like medaka, which spawn every day, sexual preference could not be reliably evaluated using the three-chamber strategy. Optimization of the protocol may solve this problem; however, the explanation for the observation that animals that are ready for spawning persist with never-accessible mating partners must be reconsidered.

The Used of Natural Anesthetic Materials Nutmeg Seed Oil in the Transportation of Sumatran Fish Fingerlings (Puntius tetrazona)

The low survival rate in Sumatran fish fingerlings transportation is a problem related to metabolic disturbances that cause death. The addition of nutmeg seed oil in the transportation medium is expected to reduce the rate of respiration and metabolism, so that fish mortality can be minimized. The purpose of this study was to determine the effective concentration of nutmeg seed oil to maintain the highest survival rate of Sumatran fish fry transported during treatment and to analyze its effect on induction time and recovery time. This research was carried out from January to March 2021 in the Ciparanje wet laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research was conducted experimentally using a factorial randomized group design (FRGD) consisting of two factors, namely the concentration of four levels (0.03, 0.06, 0.09 ml/L and control) and duration of three levels (3, 5, and 7 hours) which was repeated three times. This research uses a closed transportation system. Parameters observed were induction time and conscious recovery time of test fish, post-transportation and post-maintenance survival for 7 days and water quality consisting of temperature, DO, pH, and ammonia. The results showed that the effective nutmeg seed oil for the anesthesia of Sumatran fish fry transported was 0.03 ml/L with a transportation duration of 3 hours because it had an induction time of 08:07, recovery time of 02:41 and postoperative survival rate. transportation by 98.33% and post-maintenance for 7 days by 91.49%. Temperature parameters are 22.7oC, DO is ±10.3 mg/L, pH is ± 6.83 and ammonia is ±0.0010 mg/L.

Penambahan Asam Amino Triptofan Dalam Pakan Terhadap Tingkat Kanibalisme Dan Pertumbuhan Litopenaeus vannamei

Vannamei shrimp (Litopenaeus vannamei) is one of the leading fishery products of the fisheries sector. The problem that is often found in the failure of vannamei shrimp productions is the high level of mortality due to the nature of cannibalism during molting. One solution to minimize the cannibalism of vannamei shrimp is to provide tryptophan supplements in a feed. Tryptophan is a type of essential amino acid that serves as a precursor for serotonin biosynthesis. This study aims to determine the effect and optimal dose of tryptophan added to feed to reduce cannibalism and growth of vannamei shrimp. The test fish used in this study were vannamei shrimp with an average weight of 0,81 ± 0,26 g/individual. This study used an experimental method, a completely randomized design (CRD) consisting of 4 treatments and three replications. The test feed used in this study was artificial feed with a protein content of 38% plus tryptophan according to treatments A (0%/kg feed), B (0.75%/kg feed), C (1.5%/kg feed), and D (2.25%/kg feed). The results showed that the addition of Tryptophan in the feed had a noticeable effect (P<0,05) on cannibalism levels, survival, and molting rates but had no significant effect (P>0,05) on absolute weight growth, specific growth rates, efficiency ratios protein and efficiency feed utilization of vannamei shrimp. The best dose of tryptophan addition in feed to lower the rate of vannamei shrimp cannibalism in this study was 2,25%, capable of producing a cannibalism rate of 13,33%. Udang vaname (Litopenaeus vannamei) merupakan salah satu produk perikanan unggulan sektor perikanan. Permasalahan yang sering ditemukan dalam kegagalan produksi udang vaname adalah tingginya tingkat mortalitas karena adanya sifat kanibalisme pada saat terjadi molting. Solusi untuk meminimalisir kanibalisme udang vaname adalah dengan memberi suplemasi asam amino pada pakan, salah satunya adalah triptofan. Triptofan merupakan salah satu jenis asam amino esensial yang berfungsi sebagai prekursor untuk biosintesis serotonin. Penelitian ini bertujuan untuk mengkaji pengaruh dan dosis optimal triptofan yang ditambahkan ke dalam pakan untuk menurunkan tingkat kanibalisme dan pertumbuhan udang vaname. Ikan uji yang digunakan pada penelitian ini adalah udang vaname dengan bobot rata-rata 0,81±0,26 g/ekor. Penelitian ini menggunakan metode eksperimen, rancangan acak lengkap (RAL) yang terdiri atas 4 perlakuan dan 3 kali ulangan. Pakan uji yang digunakan dalam penelitian ini adalah pakan buatan dengan kandungan protein 38% ditambah triptofan sesuai perlakuan yaitu : A (0%/kg pakan), B (0,75%/kg pakan, C (1,5%/kg pakan), dan D (2,25%/kg pakan). Hasil penelitian menunjukkan bahwa penambahan triptofan dalam pakan berpengaruh nyata (P<0,05) terhadap tingkat kanibalisme, kelulushidupan dan tingkat molting, namun tidak berpengaruh nyata (P>0,05) terhadap pertumbuhan bobot mutlak, laju pertumbuhan spesifik, protein efisiensi rasio dan efisiensi pemanfaatan pakan. Dosis terbaik dari pemberian triptofan dalam pakan untuk menurunkan tingkat kanibalisme udang vaname dalam penelitian ini adalah 2,25%, mampu menghasilkan tingkat kanibalisme sebesar 13,33%.

Feed efficiency with the addition of probiotics for asang fish fry, Osteochilus vittatus, CV

The purpose of this study was to determine the addition of probiotics in commercial feed to the growth and survival of Asang fish (Osteochilus vittatus CV) fry. This study used an experimental method with a completely randomized design (CRD) with 4 treatments and 3 replications. The test fish used were 120 fish with a size ranging from 5-8 cm. The test fish were kept in a waring cage made of a type of paralon frame with a size of 40 x 40 x 45 cm. The treatment in this study was A (without probiotics), treatment B (addition of probiotic 10 ml/kg of feed), treatment C (addition of probiotic 20 ml/kg of feed), and treatment D (addition of probiotics 30 ml/kg of feed). From the results of this study, the highest specific growth rate was found in treatment D (6.66 ± 1.15 g), while the mean survival was 100% for all treatments. The highest FCR of fish fry was found in treatment A namely 1.43 ± 0.22, and the lowest in treatment D namely 1.12 ± 0.21, and for feed efficiency in treatment D, namely 95.24 ± 1.04 %.Keywords:Asang fishEfficiencyGrowthSurvival

PEFORMA PERTUMBUHAN IKAN KAKAP PUTIH (Lates calcarifer) PADA FASE PEMBESARAN YANG DIBERI PAKAN DENGAN PENAMBAHAN LISIN BERBEDA

This study aims to determine the best dose of the amino acid lysine in formulated feed for the growth of white snapper (Lates calcarifer) in the enlargement phase. The research design used three treatments and three replications with a completely randomized design method (CRD), namely control feed formulations with the addition of 0% lysine, P1 formulated feed with the addition of 0.2% lysine, and P2 feed formulations with 0.4% lysine. The test fish used was white snapper with an average weight of 90 grams. Fish reared in fiber tub volume of 2 m3 with the stocking 6 animals/m3. The results showed that feeding P2 formulation had a significant effect on growth in absolute weight, absolute length, daily growth rate, FCR, protein retention but had no significant effect on the survival rate and amino acid lysine retention. P2 feed (formulated feed with 0.4% lysine) can be used to support the growth of white snapper.

LAMA WAKTU PENCEGAHAN SERANGAN Aeromonas hydrophilla PADA IKAN GABUS (Channa striata) YANG DIBERI PAKAN MENGANDUNG JINTAN HITAM (Nigella sativa)

Black cumin (Nigela sativa) is immunostimulant that is able to improve the mechanism of cellular and humoral immunity response of fish. The aimed of this study was to determine the effect of the different injection times of Aeromonas hydrophilla after feeding with black cumin 3.5% for 14 days on snakehead on the prevalence, total leukocyte, hematocrit, survival rate and absolute growth of snakehead. All the test fish were fed with 3.5% black cumin for 14 days. Fish were fed three times a day at satiation. The treatment P1: injected A. hydrophilla at 10 days after feeding, P2: injected A. hydrophilla at 15 days after feeding and P3: injected with A. hydrophilla 20 days after feeding. The parameters observed were prevalence, total leukocyte, hematocrit, survival rate and absolute growth of snakehead. The results showed the treatment of the difference in injection time significantly effect on the prevalence but did not significantly effect on the survival rate and absolute growth of snakehead. Feeding containing 3.5% black cumin for 14 days can protect snakehead for 20 days after feeding. Prevalence of fish, total leukocyte, hematocrit, survival rate, length growth and weight growth fish in treatment P3, were 26.67%, 3.36 x 104sel.mm-3, 38%, 53%, 1.24 cm and 2.07 g, respectively. Keywords: A. hydrophilla, Black cumin, Snakehead.

Relationship between Upstream Swimming Behaviors of Juvenile Grass Carp and Characteristic Hydraulic Conditions of a Vertical Slot Fishway

The successful fish upstream movement through a dam/gate is closely associated with the hydraulic conditions of a fishway. To improve the passage efficiency, this study investigated the upstream swimming behaviors of juvenile grass carp, a representative fish of four major Chinese carps, under characteristic hydraulic conditions of a designed vertical slot fishway model. The impacts of different discharges and baffle lead angles on the successful movement of test fish were analyzed, and the selection of the movement trajectory was studied through overlay of their upstream swimming trajectories on the water flow field resulting from numerical modeling. We found that under the same discharge, the percentage of successful test fish movement with a lead angle of 45° was higher than 60° and 30°. Within a fixed lead angle, the higher the discharge, the lower the percentage of successful movement. During upstream movement, the test fish had a preferred water velocity of 0.01–0.45 m/s in the pool, and avoided areas where the turbulence kinetic energy (TKE) was greater than 0.012 m2/s2. These results provide a basis for the hydraulic design of vertical slot fishways and a reference for studying swimming behaviors of other fish species.