Factors influencing environmental sampling recovery of healthcare pathogens from non-porous surfaces with cellulose sponges

Results from sampling healthcare surfaces for pathogens are difficult to interpret without understanding the factors that influence pathogen detection. We investigated the recovery of four healthcare-associated pathogens from three common surface materials, and how a body fluid simulant (artificial test soil, ATS), deposition method, and contamination levels influence the percent of organisms recovered (%R). Known quantities of carbapenemase-producing KPC+ Klebsiella pneumoniae (KPC), Acinetobacter baumannii, vancomycin-resistant Enterococcus faecalis, and Clostridioides difficile spores (CD) were suspended in Butterfield’s buffer or ATS, deposited on 323cm2 steel, plastic, and laminate surfaces, allowed to dry 1h, then sampled with a cellulose sponge wipe. Bacteria were eluted, cultured, CFU counted and %R determined relative to the inoculum. The %R varied by organism, from <1% (KPC) to almost 60% (CD) and was more dependent upon the organism’s characteristics and presence of ATS than on surface type. KPC persistence as determined by culture also declined by >1 log10 within the 60 min drying time. For all organisms, the %R was significantly greater if suspended in ATS than if suspended in Butterfield’s buffer (p<0.05), and for most organisms the %R was not significantly different when sampled from any of the three surfaces. Organisms deposited in multiple droplets were recovered at equal or higher %R than if spread evenly on the surface. This work assists in interpreting data collected while investigating a healthcare infection outbreak or while conducting infection intervention studies.

Testing the accuracy of Soil Testing Kit® Transchem

Soil testing is key to soil fertility management as it serves as a fertilizer application guide to farmers, scientists and consultants. It gives information on soil nutrient status and its supplying capacity. Laboratory (LB) procedures have been the most reliable approach for soil nutrients analyses. However, it is costly and nonpoint. Thus, the use of in–situ testing kit emerges and becomes prominent. Notwithstanding, applicability of soil testing kit must be validated by laboratory test. This work aimed to examine the reliability/suitability of Soil Testing Kit® Transchem (SK) in determining selected soil nutrients in Sahel Savannah, Nigeria. Twentyfive replicate soil samples were collected from 12°47’86’’-12°20’96’’N and 4°38’37’’-4°188’02’’E, Kebbi State Nigeria and used to test soil pH, N, P, K and soil organic carbon (SOC) by SK and LB. The SK uses colour chart and comparator for rating nutrients status qualitatively into; low, medium and high and up to very high for P. The LB results were transformed to qualitative data by corresponding the values with soil rating standardinto low, medium and high. To perform statistics, weighting was done by assigning weight load to each category; low = 1, medium = 2 and high = 3. The two methods were compared using t-test, regression and descriptive analyses. Results showed non-significant difference between the two methods for soil contents of N, P and K. However, SK poorly estimated soil pH and SOC. Correlation and regression coefficients (r = 0.915 and R2 = 0.838, respectively) indicated reliability of the SK. It is concluded that SK can be reliably used for N, P, and K but not soil pH and SOC estimation for soils in Sahel savannah of Nigeria.

Effect of Plasticizers on the Physicochemical properties of Bioplastic Extracted from Banana Peels

Plasticizers are the binding substances used to increase the elasticity of materials. In this research work, bioplastic is extracted from banana peels using various plasticizers such as, glycerol, urea, distilled water and glucose. The prepared bioplastics were characterized by using Fourier-transform infrared spectroscopy (FTIR) spectroscopic analysis which showed that the peak at 3355 cm-1 indicate the H-bonding formation between N-H urea and starch. The physicochemical properties such as water absorption test, soil decomposition and load test of synthesized bioplastics were analyzed at ambient temperature. The water uptake analysis showed that bioplastic absorbs water for up to 4 days without being decay. The load test showed that urea plasticized bioplastic has a high tensile strength of 2.3 KPa. The result revealed that the bioplastic with glucose as a plasticizer showed the effective result in water uptake and soil decomposition test whereas the urea plasticized bioplastic showed relatively good tensile strength.

Ionic Liquid Extraction Behavior of Cr(VI) Absorbed on Humic Acid–Vermiculite

Cr(VI) can be released into soil as a result of mining, electroplating, and smelting operations. Due to the high toxicity of Cr(VI), its removal is necessary in order to protect ecosystems. Vermiculite is applied in situations where there is a high degree of metal pollution, as it is helpful during the remediation process due to its high cation exchange capacity. The Cr(VI) contained in the vermiculite should be extracted in order to recover it and to reduce the impact on the environment. In this work, adsorption equilibrium data for Cr(VI) in a simulated sorbent for soil remediation (a mixture that included both humic acid (HA) and vermiculite) were a good fit with the Langmuir isotherm model. The simulated sorbent for soil remediation was a favorable sorbent for Cr(VI) when it was in the test soil. An ionic liquid, [C4mim]Cl (1-butyl-3-methylimidazolium chloride), was studied to determine its efficiency in extracting Cr(VI) from the Cr- contaminated simulated sorbent in soil remediation. At 298 K and within 30 min, approximately 33.48 ± 0.79% of Cr(VI) in the simulated sorbent in soil remediation was extracted into [C4mim]Cl. Using FTIR spectroscopy, the absorbance intensities of the bands at 1032 and 1010 cm−1, which were attributed to C-O bond stretching in the polysaccharides of HA, were used to detect the changes in HA in the Cr-contaminated simulated sorbent for soil remediation before and after extraction. The results showed that Cr(VI) that has been absorbed on HA can be extracted into [C4mim]Cl. Using 1H NMR, it was observed that the 1-methylimizadole of [C4mim] Cl played an important role in the extraction of Cr(VI), which bonded with HA on vermiculite and was able to be transformed into the [C4mim]Cl phase.

Effects of Plastic Pollution of Soil on the Growth and Survival of Bacteria and Fungi

The study examined the effect of plastic waste on soil bacteria and fungi. The test soil samples were collected from Lokoja international market waste dump site and the control soil sample was collected from non plastic contaminated garden in Salem University, Lokoja. The samples were analysed using Gas chromatography with mass spectrometer. The test soil sample soil sample had high quantity of plastic contaminant which were Methylene chloride 17.45mg/kg, hexane 10.05mg/kg, chloroform 1.56mg/kg, toluene 5.87mg/kg, tetrachloroethylene 1.48mg/kg as compared to the control garden soil sample, which had methylene chloride 0.54mg/kg, hexane 0.26mg/kg, chloroform 0.31mg/kg, toluene 5.87mg/kg and tetrachloroethylene 0.01mg/kg. The result showed the presence of plastic in the soil and it effect on bacteria and fungi. The totals of 11 bacteria were isolated from both soil samples using nutrient agar. The bacteria isolated are; Corynebacterium spp. (12%), Enterobacter spp. (8%), Acinetobacter spp. (6%), Escherichia coli (16%), Epidermis, Bacillus subtilis (15%), Serratia sp. (8%), Proteus spp. (4%), Micrococcus luteus (7%), Flavobacterium spp. (10%), Pseudomonas aeruginosa (15%). Micrococcus luteus, Flavobacterium spp. and Pseudomonas aeruginose were not isolated in plastic composted soil sample due to the presence of plasticizers. The total of 6 fungi were isolated, namely Penicilliun expansion (12%), Sacchromyces sp. (24%), Aspergillus niger (19%), Fusarium spp. (20%), Rhizopus stolonifer (10%) and Mucor piriformis (15%). This study reveals the effect of plastic waste, as it inhibits the growth of microorganism that is important for soil activities, thereby reducing the soil nutrients, fertility and productivity.

The Experimental Investigation of the Repeated-Loading Behaviour of the Sand-Rubber-Mixture (SRM)

Nowadays the waste rubber problems are concerned due to the environmental issues, storage, and recycling difficulty. However, the rubber base equipment has been widely used to protect structures for vibrations - that has been generated by the structure or induced from the vicinity area or the bedrock into the structure - due to the notable capability of absorbing energy. In this study, the repeated-loading behaviour of the Sand Rubber Mixture (SRM) has been investigated and the remarkable energy absorption properties of the mixture have been illustrated. The test soil material that has been used in this study was a well-graded sand (SW) with a mean grain size of 2 mm. The test martial rubber that has been used was grain particles with a uniform size of 4.76 mm. The sand rubber mixture (SRM) was prepared by using 7.5% rubber inclusion because it was found as the optimum rubber content. A series of force control repeated-loading CBR tests have been arranged. The effect of mixing rubber particles with the well-graded sand (SW test material) has been investigated. This shows the remarkable energy absorption capability of Sand Rubber Mixture (SRM) to protect the bed of a machine’s footing that is generating repeated loads. The SRM usage could be extended to be employed as a part of an energy absorption unit and dampers facilities beneath a machine footing or structures that are sensitive to the vibration to prevent destructive deformation and resonance phenomenon.

A CFD Porous Materials Model to Test Soil Enriched with Nanostructured Zeolite Using ANSYS-Fluent(™)

Soil health is a great concern worldwide due to the huge variety of pollutants and human activities that may cause damage. There are different ways to remediate and make a better use of soil and a choice may be using zeolite in activities like gardening, farming, environment amending, among others. In this work is proposed a model to simulate how mixing zeolite with soil may be beneficial in different ways, we are especially interested in interactions of mixed soil-zeolite with water. This model is based in different flow regimes where water interacts with two layers formed by nanostructured zeolite and soil in a vertical arrangement. The analysis is approached as a bi-layer porous material model resolved by using the mathematical model implemented in ANSYS-Fluent. Such model uses a multi-fluid granular model to describe the flow behavior of a fluid–solid mixture where all the available interphase exchange coefficient models are empirically based. Despite the great capabilities of numerical simulation tools, it is known that at present time, the literature lacks a generalized formulation specific to resolve this kind of phenomena where a porous media is analyzed. This model is developed to obtain a systematic methodology to test nanomaterials with porous features produced in our laboratory which is the next step for near future work within our research group.

Pemanfaatan Semen Slag untuk Stabilisasi Tanah dan Pengaruhnya Terhadap Nilai CBR Berdasarkan Variasi Kadar Air Sisi Basah Optimum

Many of the damage in road construction occurred because the soil did not have a good bearing capacity, therefore soil improvement was needed to increase the strength of the soil. One of the damaged roads that occur due to the lack of soil bearing capacity is at Priyayi Mosque Village Road, Kasemen District, Serang City which has a low carrying capacity with a CBR value of 1.99%. In this study, several tests were carried out, soil physical and California Bearing Ratio test. Soil physical tests carried out were water content, soil density, grain size analysis, Atterberg limits, compaction test and, CBR with the addition of slag cement with variations of 0%, 5%, 10%, 15% based on Variation of optimum wet side moisture content. The result of soil physical properties testing obtained the type of soil is an organic clay with high plasticity. The results of the California Bearing Ratio test on the original soil had a CBR value of 2.4% and there was an increase to the optimum at the addition of 15% Slag Cement in 0 and 3 days of curing with a CBR value of 11.2% and 15.25%. Based on these results, it can be concluded that the cement slag content of 15% with 3 days of curing time can increase the bearing capacity of the soil and fulfill the requirements as a good road subgrade

Phylogenetic dependence of plant-soil feedback promotes rare species in a subtropical forest

The widespread observation that rare species have stronger conspecific plant-soil feedback (PSF) than common species raises more questions than answers on how rare species can possibly win the dance with abundant species. Here, we test soil feedback effect of phylogenetically related species on seedlings of contrasting local abundance in a subtropical forest. The results showed that although rare species suffered strong negative PSF in soils of conspecifics or phylogenetically close relatives, no such feedback was found in the soils of distant relatives. In contrast, although common species had weak conspecific PSF, they suffered consistently strong heterospecific soil feedback. These mechanisms ensure that rare species would fare well in the neighborhood of phylogenetically distant heterospecifics but do poorly under their close relatives, while common species perform relatively well in their own neighborhood but poorly in others’. This phylogenetic conservatism in PSF facilitates the persistence of rare species in a community.