Oil Red O based method for Exosome Labelling and Detection

With the realization of the role of exosomes in diseases especially cancer, exosome research is gaining popularity in biomedical sciences. To understand exosome biology, their labelling and tracking studies are important. New and improved methods of exosome labelling for detection and tracking of exosomes need to be developed to harness their therapeutic and diagnostic potential. In this paper, we report a novel, simple and effective method of labelling and detecting exosomes using Oil red O (ORO) which is a dye commonly used for lipid staining. Using ORO is a cost effective and easy approach with intense red colouration of stained exosomes. Further, the issues faced with commonly used lipophilic dyes for exosomes labelling such as long term persistence of dyes, aggregation and micelle formation of dyes, difficulty to distinguish dye particles from labelled exosomes and detection of large aggregates of dye or dye-exosome are not seen with ORO dye. This method shows good labelling efficiency of exosomes with very sensitive detection and real-time tracking of the cellular uptake of exosomes.

Multifunctional Platinum(IV) and Cyanine Dye-based Polyprodrug for Trimodal Imaging-Guided Chemo-Phototherapy

Imaging-guided chemo-phototherapy based on a single nanoplatform has a great significance to improve the efficiency of cancer therapy and diagnosis. However, high drugs content, no burst release and real-time tracking...

Human identification and tracking using ultra-wideband-vision data fusion in unstructured environments

<p class="Abstract">Nowadays, the importance of working in changing and unstructured environments such as logistics warehouses through the cooperation between Automated Guided Vehicles (AGV) and the operator is increasingly demanded. The challenge addressed in this article aims to solve two crucial functions of autonomy: operator identification, and tracking. These tasks are necessary to enable an AGV to follow the selected operator along his path. This paper presents an innovative, accurate, robust, autonomous, and low-cost operator real-time tracking system, leveraging the inherent complementarity of the uncertainty regions (2D ellipses) between ultra-wideband (UWB) transceivers and cameras. The test campaign shows how the UWB system has higher uncertainty in the angular direction. In contrast, in the case of the vision system, the uncertainty is predominant along the radial coordinate. Due to the nature of the data, a sensor fusion demonstrates improvement in the accuracy and goodness of the final tracking.</p>

O-OGC03 Real-time tracking and classification of tumour and non-tumour tissue in upper gastrointestinal cancer specimens using diffuse reflectance spectroscopy

Background Diffuse reflectance spectroscopy (DRS) is a technique that allows discrimination of normal and abnormal tissue based on spectral data. It is a promising technique for cancer margin assessment. However, application in a clinical setting is limited by the inability of DRS to mark the tissue that has been scanned and its lack of continuous real-time spectral measurements. This aim of this study was to develop a real-time tracking system to enable localisation of the tip of a handheld DRS probe to aid classification of tumour and non-tumour tissue. Methods A coloured marker was attached to the DRS fibre probe and was detected using colour segmentation. A Kalman filter was used to estimate the probe’s tip position during scanning of the tissue specimen. In this way, the system was robust to partial occlusion allowing real-time detection and tracking. Supervised classification algorithms were used for the discrimination between tumour and non-tumour tissue, and evaluated in terms of overall accuracy, sensitivity, specificity, and the area under the curve (AUC). A live augmented view with all the tracked and classified optical biopsy sites were presented, providing visual feedback to the surgeons. Results A green coloured marker was successfully used to track the DRS probe. The measured root mean square error of probe tip tracking was 1.18±0.58mm and 1.05±0.28mm for the X and Y directions, respectively, whilst the maximum measured error was 1.76mm. Overall, 47 distinct sets of tumour and non-tumour tissue data were recorded through real-time tracking of ex vivo oesophageal and gastric tissue. The overall diagnostic accuracy of the system to classify tumour and non-tumour tissue in real-time was 94% for stomach and 96% for the oesophagus. Conclusions We have been able to successfully develop a real-time tracking system for a DRS probe when used on stomach and oesophageal tissue for tumour detection, and the accuracy derived demonstrates the strength and clinical value of the technique. The method allows real-time tracking and classification with short data acquisition time to aid margin assessment in cancer resection surgery.

A new innovative real-time tracking method for flying insects applicable under natural conditions

Background Sixty percent of all species are insects, yet despite global efforts to monitor animal movement patterns, insects are continuously underrepresented. This striking difference between species richness and the number of species monitored is not due to a lack of interest but rather to the lack of technical solutions. Often the accuracy and speed of established tracking methods is not high enough to record behavior and react to it experimentally in real-time, which applies in particular to small flying animals. Results Our new method of real-time tracking relates to frequencies of solar radiation which are almost completely absorbed by traveling through the atmosphere. For tracking, photoluminescent tags with a peak emission (1400 nm), which lays in such a region of strong absorption through the atmosphere, were attached to the animals. The photoluminescent properties of passivated lead sulphide quantum dots were responsible for the emission of light by the tags and provide a superb signal-to noise ratio. We developed prototype markers with a weight of 12.5 mg and a diameter of 5 mm. Furthermore, we developed a short wave infrared detection system which can record and determine the position of an animal in a heterogeneous environment with a delay smaller than 10 ms. With this method we were able to track tagged bumblebees as well as hawk moths in a flight arena that was placed outside on a natural meadow. Conclusion Our new method eliminates the necessity of a constant or predictable environment for many experimental setups. Furthermore, we postulate that the developed matrix-detector mounted to a multicopter will enable tracking of small flying insects, over medium range distances (>1000m) in the near future because: a) the matrix-detector equipped with an 70 mm interchangeable lens weighs less than 380 g, b) it evaluates the position of an animal in real-time and c) it can directly control and communicate with electronic devices.

Assistive Devices: Technology Development for the Visually Impaired

Technology has been contributing significantly to the development of assistive devices for disabled persons (DPs). Many of these devices aim to assist people who are blind or visually impaired, providing them with friendlier ways to interact with their surroundings (obstacles, objects, and navigation). However, the high cost of these devices makes it difficult for DPs to purchase them. The development of an assistive device kit to be used by the visually impaired in controlled environments (indoor) or urban spaces (outdoor) is presented in this work with a didactic and detailed approach. This low-cost kit (USD 50 per device) consists of a Smart Cane, Smart Cap, and Smart Glove. The methodology addressed aims to support and encourage the reproduction, customization, and manufacture of new assistive devices with low cost and wide applicability. The devices are functional, benefit usability, and, due to the characteristics of the project and the materials used in their construction, do not impact the ergonomics of the visually impaired or disabled person who may use these devices. The devices that integrate the assistive kit can be used independently or combined. In addition to having detection, fall-control, navigation, and real-time tracking functions, they are independent of additional local infrastructure for their use.

Experimental Validation of Real-Time Ski Jumping Tracking System Based on Wearable Sensors

For sports scientists and coaches, its crucial to have reliable tracking systems to improve athletes. Therefore, this study aimed to examine the validity of a wearable real-time tracking system (WRRTS) for the quantification of ski jumping. The tracking system consists of wearable trackers attached to the ski bindings of the athletes and fixed antennas next to the jumping hill. To determine the accuracy and precision of the WRRTS, four athletes of the German A or B National Team performed 35 measured ski jumps. The WRRTS was used to measure the 3D positions and ski angles during the jump. The measurements are compared with camera measurements for the in-flight parameters and the official video distance for the jumping distance to assess their accuracy. We statistically evaluated the different methods using Bland–Altman plots. We thereby find a mean absolute error of 0.46 m for the jumping distance, 0.12 m for the in-flight positions, and 0.8°, and 3.4° for the camera projected pitch and V-style opening angle, respectively. We show the validity of the presented WRRTS to measure the investigated parameters. Thus, the system can be used as a tracking system during training and competitions for coaches and sports scientists. The real-time feature of the tracking system enables usage during live TV broadcasting.